LOCUS ALG11-pGEc2-DEST 6697 bp DNA circular 21-JAN-2013 FEATURES Location/Qualifiers misc_feature complement(6175..6304) /note="3'-ITR" misc_feature complement(5873..6087) /note="BGH pA" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" CDS 129..989 /dnas_title="AmpR" /label=AmpR /vntifkey="4" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_feature 5325..5866 /note="WPRE" misc_recomb complement(2974..3001) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_feature 4504..4526 /note="attB2" misc_feature 5304..5309 /note="BstB1 site" misc_feature 4527..5222 /note="Super GFP" misc_feature 5223..5273 /note="AviTag" misc_feature 5274..5297 /note="His Tag" PCR_frame 3002..4504 /note="Reading Frame" /set="Set 1" source 3006..4481 /dnas_title="ALG11-pGEc2-DEST" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:167122 IMAGE:8860455" /tissue_type="Clones donated by RIKEN" /clone_lib="NIH_MGC_424" /lab_host="DH10B" /note="Vector: pFLC1" gene 3006..4481 /dnas_title="ALG11" /gene="ALG11" /gene_synonym="GT8" /gene_synonym="UTP14C" /db_xref="GeneID:440138" /db_xref="HGNC:32456" CDS 3006..4481 /dnas_title="asparagine-linked glycosylation 11, alpha-1,2-mannosyltransferase homolog (yeast)" /gene="ALG11" /gene_synonym="GT8" /gene_synonym="UTP14C" /codon_start=1 /product="asparagine-linked glycosylation 11, alpha-1,2-mannosyltransferase homolog (yeast)" /protein_id="AAI42999.1" /db_xref="GI:148745765" /db_xref="GeneID:440138" /db_xref="HGNC:32456" /translation="MAAGERSWCLCKLLRFFYSLFFPGLIVCGTLCVCLVIVLWGIRL LLQRKKKLVSTSKNGKNQMVIAFFHPYCNAGGGGERVLWCALRALQKKYPEVVYVVYT GDVNVNGQQILEGAFRRFNIRLIHPVQFVFLRKRYLVEDSLYPHFTLLGQSLGSIFLG WEALMQCVPDVYIDSMGYAFTLPLFKYIGGCQVGSYVHYPTISTDMLSVVKNQNIGFN NAAFITRNPFLSKVKLIYYYLFAFIYGLVGSCSDVVMVNSSWTLNHILSLWKVGNCTN IVYPPCDVQTFLDIPLHEKKMTPGHLLVSVGQFRPEKNHPLQIRAFAKLLNKKMVESP PSLKLVLIGGCRNKDDELRVNQLRRLSEDLGVQEYVEFKINIPFDELKNYLSEATIGL HTMWNEHFGIGVVECMAAGTIILAHNSGGPKLDIVVPHEGDITGFLAESEEDYAETIA HILSMSAEKRLQIRKSARASVSRFSDQEFEVTFLSSVEKLFK" misc_difference 3292..3292 /dnas_title="ALG11" /gene="ALG11" /gene_synonym="GT8" /gene_synonym="UTP14C" /note="'T' in cDNA is 'C' in the human genome; amino acid difference: 'V' in cDNA, 'A' in the human genome. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." misc_feature 3006..4481 /note="ALG11 coding region" misc_feature 4482..4502 /note="TEV site" PCR_primer 2992..3001 /dnas_title="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /primer="CAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" PCR_primer complement(3002..4511) /dnas_title="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original="ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG" PCR_product complement(2992..4511) /pair="Pair 1" /set="Set 1" /current=1 /score="1.000" vector join(4512..6697,1..2991) /source="pGEc2-DEST" /type="Destination Vector" clone 2992..4511 /source="ALG11-C-term-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCATCACAA GTTTGTACAA AAAAGCAGGC 3001 TCGCCatggc ggccggcgaa aggagctggt gcctgtgcaa gttgttgagg tttttttatt 3061 cattattctt ccctgggctc attgtatgtg gaactttatg tgtgtgtttg gtcattgtcc 3121 tttggggaat cagactgctg ctacagagaa agaaaaaatt agtgtcaact agcaaaaatg 3181 ggaaaaatca aatggtgatt gcattttttc atccatactg caatgctggt ggaggaggag 3241 aaagagtttt atggtgtgct ttaagagccc tgcagaaaaa gtatcctgaa gtagtttatg 3301 ttgtttatac cggcgatgtt aatgtcaacg gtcaacagat actagaaggt gctttcagaa 3361 gatttaacat cagattaatt cacccagtgc agtttgtttt tttaaggaaa cgctatcttg 3421 tggaagattc actgtatcct cacttcacac tgctgggcca aagtctagga tccatttttc 3481 ttggctggga agctctaatg cagtgtgttc ctgatgttta cattgattca atgggatacg 3541 cttttacgct tcctctgttt aagtatatag ggggttgcca agttggaagc tatgttcatt 3601 atcctactat cagcaccgac atgctctctg tagtgaagaa tcaaaatatt ggatttaata 3661 atgcagcctt cattaccagg aatccttttc tcagcaaagt aaagctcatc tactactatt 3721 tatttgcttt tatttatgga cttgttggtt cttgcagtga tgtagtcatg gtcaattctt 3781 cttggacact aaaccatatt ctctcactat ggaaagttgg gaattgcact aacattgttt 3841 atccaccttg tgatgtgcag acatttctgg acattccctt acatgagaaa aagatgaccc 3901 caggacattt gctggtttct gttggccagt ttaggccgga aaagaatcat ccattgcaga 3961 tcagagcctt tgctaaattg ctgaataaga agatggttga gtcacctcct tcgcttaaac 4021 ttgtcctcat tggaggttgt cgtaacaaag atgatgaact tagggtaaac caactgagaa 4081 ggctgtctga ggatttagga gttcaagaat atgtggaatt taaaataaac attccatttg 4141 atgaattaaa gaattatttg tctgaagcaa caattggtct gcataccatg tggaacgagc 4201 attttgggat tggagttgtg gagtgtatgg cagctggcac aattatcctt gcacacaatt 4261 cggggggccc aaagcttgac attgtggttc ctcacgaagg agatataact ggctttctgg 4321 ctgagagtga agaagactat gctgaaacta tcgctcacat tctttccatg tctgcagaaa 4381 agagactcca aatcagaaaa agtgctcgtg catctgtaag cagattctct gatcaggaat 4441 ttgaagtgac attcctatca tctgtggaaa agttatttaa ggaaaacttg tactttcaag 4501 gctCCAGCTT TCTTGTACAA AGTGGTATGA GCAAGGGCGA GGAACTGTTC ACCGGCGTGG 4561 TGCCCATCCT GGTGGAACTG GATGGCGACG TGAACGGCCA CAAGTTCTCT GTGCGGGGAG 4621 AGGGCGAAGG CGACGCCACA AATGGCAAGC TGACCCTGAA GTTCATCTGC ACCACCGGCA 4681 AGCTGCCCGT GCCTTGGCCT ACCCTCGTGA CCACACTGAC CTACGGCGTG CAGTGCTTCA 4741 GCAGATACCC CGACCATATG AAGCGGCACG ACTTCTTCAA GAGCGCCATG CCCGAGGGCT 4801 ACGTGCAGGA ACGGACCATC AGCTTCAAGG ACGACGGCAC CTACAAGACC AGAGCCGAAG 4861 TGAAGTTCGA GGGCGACACC CTCGTGAACC GGATCGAGCT GAAGGGCATC GATTTCAAAG 4921 AGGACGGCAA CATCCTGGGC CACAAGCTGG AGTACAACTT CAACAGCCAC AACGTGTACA 4981 TCACCGCCGA CAAGCAGAAG AACGGCATCA AGGCCAACTT CAAGATCCGG CACAACGTGG 5041 AAGATGGCAG CGTGCAGCTG GCCGACCACT ACCAGCAGAA CACCCCCATC GGAGATGGCC 5101 CCGTGCTGCT GCCCGACAAC CACTACCTGA GCACCCAGAG CGTGCTGAGC AAGGACCCCA 5161 ACGAGAAGCG GGACCACATG GTGCTGCTGG AATTCGTGAC CGCCGCTGGC ATCACCCACG 5221 GCATGAGCGG CCTGAACGAC ATCTTCGAGG CCCAGAAAAT CGAGTGGCAC GAGCACCACC 5281 ATCATCACCA TCACCACTGA TGATTCGAAA AATAATAAGG ATCCAATCAA CCTCTGGATT 5341 ACAAAATTTG TGAAAGATTG ACTGGTATTC TTAACTATGT TGCTCCTTTT ACGCTATGTG 5401 GATACGCTGC TTTAATGCCT TTGTATCATG CTATTGCTTC CCGTATGGCT TTCATTTTCT 5461 CCTCCTTGTA TAAATCCTGG TTGCTGTCTC TTTATGAGGA GTTGTGGCCC GTTGTCAGGC 5521 AACGTGGCGT GGTGTGCACT GTGTTTGCTG ACGCAACCCC CACTGGTTGG GGCATTGCCA 5581 CCACCTGTCA GCTCCTTTCC GGGACTTTCG CTTTCCCCCT CCCTATTGCC ACGGCGGAAC 5641 TCATCGCCGC CTGCCTTGCC CGCTGCTGGA CAGGGGCTCG GCTGTTGGGC ACTGACAATT 5701 CCGTGGTGTT GTCGGGGAAG CTGACGTCCT TTCCATGGCT GCTCGCCTGT GTTGCCACCT 5761 GGATTCTGCG CGGGACGTCC TTCTGCTACG TCCCTTCGGC CCTCAATCCA GCGGACCTTC 5821 CTTCCCGCGG CCTGCTGCCG GCTCTGCGGC CTCTTCCGCG TCTTCGAGAT CTGCCTCGAC 5881 TGTGCCTTCT AGTTGCCAGC CATCTGTTGT TTGCCCCTCC CCCGTGCCTT CCTTGACCCT 5941 GGAAGGTGCC ACTCCCACTG TCCTTTCCTA ATAAAATGAG GAAATTGCAT CGCATTGTCT 6001 GAGTAGGTGT CATTCTATTC TGGGGGGTGG GGTGGGGCAG GACAGCAAGG GGGAGGATTG 6061 GGAAGACAAT AGCAGGCATG CTGGGGACTC GAGTTAAGGG CGAATTGCCG ATTAGGATCT 6121 TCCTAGAGCA TGGCTACGTA GATAAGTAGC ATGGCGGGTT AATCATTAAC TACAAGGAAC 6181 CCCTAGTGAT GGAGTTGGCC ACTCCCTCTC TGCGCGCTCG CTCGCTCACT GAGGCCGGGC 6241 GACCAAAGGT CGCCCGACGC CCGGGCTTTG CCCGGGCGGC CTCAGTGAGC GAGCGAGCGC 6301 GCAGCCTTAA TTAAGAGCTC ATGGCGCGCC TAGGCCTTGA CGGCCTTCCG CCAATTCGCC 6361 CTATAGTGAG TCGTATTACG TCGCGCTCAC TGGCCGTCGT TTTACAACGT CGTGACTGGG 6421 AAAACCCTGG CGTTACCCAA CTTAATCGCC TTGCAGCACA TCCCCCTTTC GCCAGCTGGC 6481 GTAATAGCGA AGAGGCCCGC ACCGAAACGC CCTTCCCAAC AGTTGCGCAG CCTGAATGGC 6541 GAATGGGAGC GCCCTGTAGC GGCCACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA 6601 AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA 6661 CGCGAATTTT AACAAAATAT TAACGCTTAC AATTTAG //