LOCUS GTDC1-pGEc1-DEST 5614 bp DNA circular 21-JAN-2013 FEATURES Location/Qualifiers misc_feature complement(5092..5221) /note="3'-ITR" misc_feature complement(4790..5004) /note="BGH pA" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" CDS 129..989 /dnas_title="AmpR" /label=AmpR /vntifkey="4" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_feature 4242..4783 /note="WPRE" misc_recomb complement(2974..3001) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_recomb 4147..4169 /label=attB2 /note="attB2 site" misc_feature 4179..4202 /note="8xHis Tag" misc_feature 4206..4229 /note="StrepTag" PCR_frame 3002..4147 /note="Reading Frame" /set="Set 1" source 3006..4124 /dnas_title="GTDC1-pGEc1-DEST" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:21948 IMAGE:4390876" /tissue_type="Bone, osteosarcoma" /clone_lib="NIH_MGC_86" /lab_host="DH10B" /note="Vector: pCMV-SPORT6" gene 3006..4124 /dnas_title="GTDC1" /gene="GTDC1" /gene_synonym="mat-Xa" /db_xref="GeneID:79712" /db_xref="HGNC:20887" /db_xref="MIM:610165" CDS 3006..4124 /dnas_title="glycosyltransferase-like domain containing 1" /gene="GTDC1" /gene_synonym="mat-Xa" /codon_start=1 /product="glycosyltransferase-like domain containing 1" /protein_id="AAH17741.1" /db_xref="GI:34190921" /db_xref="GeneID:79712" /db_xref="HGNC:20887" /db_xref="MIM:610165" /translation="MSILIIEAFYGGSHKQLVDLLQEELGDCVVYTLPAKKWHWRART SALYFSQTIPISEHYRTLFASSVLNLTELAALRPDLGKLKKILYFHENQLIYPVKKCQ ERDFQYGYNQILSCLVADVVVFNSVFNMESFLTSMGKFMKLIPDHRPKDLESIIRPKC QVIYFPIRFPDVSRFMPKHKTTHLKKMLGLKGNGGAVLSMALPFQPEQRDSEDLLKNF NSECDTHCGLDTARQEYLGNSLRQESDLKKSTSSDNSSSHHGENKQNLTVDPCDILGG VDNQQRLLHIVWPHRWLEAVYCGCYPLCPKDLVYPEIFPAEYLYSTPEQLSKRLQNFC KRPDIIRKHLYKGEIAPFSWAALHGKFRSLLTTEPREDL" misc_feature 3006..4124 /note="GTDC1 (isoform 2) coding region " misc_feature 4125..4145 /note="TEV site" PCR_primer 2992..3001 /dnas_title="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /primer="CAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" PCR_primer complement(3002..4154) /dnas_title="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original="ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG" PCR_product complement(2992..4154) /pair="Pair 1" /set="Set 1" /current=1 /score="1.000" vector join(4155..5614,1..2991) /source="pGEc1-DEST" /type="Destination Vector" clone 2992..4154 /source="GTDC1-C-term-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCATCACAA GTTTGTACAA AAAAGCAGGC 3001 TCGCCatgag tatcctcatc attgaagcat tctatggagg ctcccataaa cagctggtgg 3061 atcttcttca agaagagtta ggagactgtg tcgtttatac ccttcctgca aagaaatggc 3121 attggagagc ccggacatct gctttatatt tctctcagac cattcccatc agtgagcatt 3181 acaggaccct ctttgcaagt tcagtgctta acctgaccga actggctgcc cttcggcctg 3241 accttgggaa actgaaaaag attctgtatt ttcacgagaa ccagttgata tatcctgtca 3301 agaaatgtca ggagagggat ttccaatatg gatacaacca aattctttca tgcctggtgg 3361 ctgatgtggt tgtattcaac tcagttttta atatggaatc atttctcact tccatgggaa 3421 aatttatgaa gctgattcct gatcacagac ccaaggatct ggaaagcatc atcagaccca 3481 agtgccaagt tatttacttt cccatcaggt ttcctgatgt gagcagattc atgcccaagc 3541 acaaaacaac ccatttaaag aagatgctcg gccttaaagg aaatggcggt gcggttctgt 3601 ccatggccct tccttttcag ccagagcaga gagattcaga ggatttattg aagaatttta 3661 attcagagtg tgatacacac tgtggccttg atactgcacg acaagaatat ttgggtaact 3721 cattaagaca ggaatcagac ttgaaaaaat ccacctcgtc agataattca agctctcatc 3781 atggtgaaaa taaacaaaat ctgactgttg atccctgtga cattttgggt ggagttgata 3841 atcagcaaag actgctacac attgtctggc ctcacaggtg gttggaagct gtgtactgtg 3901 ggtgttaccc actttgtcct aaagatttgg tttatcccga aatatttcca gctgaatatc 3961 tgtattctac acctgaacag ctttcaaaaa ggctccagaa tttctgcaag agaccagata 4021 ttataagaaa acatctctat aagggtgaaa tcgctccgtt ttcttgggca gccctacatg 4081 gtaaattcag gtctctgctt acaacagaac ctagggaaga tttggaaaac ttgtactttc 4141 aaggctCCAG CTTTCTTGTA CAAAGTGGTG ATGGCCGCCA TCATCATCAT CATCATCATC 4201 ATGCGTGGAG CCACCCGCAG TTCGAAAAAT AATAAGGATC CAATCAACCT CTGGATTACA 4261 AAATTTGTGA AAGATTGACT GGTATTCTTA ACTATGTTGC TCCTTTTACG CTATGTGGAT 4321 ACGCTGCTTT AATGCCTTTG TATCATGCTA TTGCTTCCCG TATGGCTTTC ATTTTCTCCT 4381 CCTTGTATAA ATCCTGGTTG CTGTCTCTTT ATGAGGAGTT GTGGCCCGTT GTCAGGCAAC 4441 GTGGCGTGGT GTGCACTGTG TTTGCTGACG CAACCCCCAC TGGTTGGGGC ATTGCCACCA 4501 CCTGTCAGCT CCTTTCCGGG ACTTTCGCTT TCCCCCTCCC TATTGCCACG GCGGAACTCA 4561 TCGCCGCCTG CCTTGCCCGC TGCTGGACAG GGGCTCGGCT GTTGGGCACT GACAATTCCG 4621 TGGTGTTGTC GGGGAAGCTG ACGTCCTTTC CATGGCTGCT CGCCTGTGTT GCCACCTGGA 4681 TTCTGCGCGG GACGTCCTTC TGCTACGTCC CTTCGGCCCT CAATCCAGCG GACCTTCCTT 4741 CCCGCGGCCT GCTGCCGGCT CTGCGGCCTC TTCCGCGTCT TCGAGATCTG CCTCGACTGT 4801 GCCTTCTAGT TGCCAGCCAT CTGTTGTTTG CCCCTCCCCC GTGCCTTCCT TGACCCTGGA 4861 AGGTGCCACT CCCACTGTCC TTTCCTAATA AAATGAGGAA ATTGCATCGC ATTGTCTGAG 4921 TAGGTGTCAT TCTATTCTGG GGGGTGGGGT GGGGCAGGAC AGCAAGGGGG AGGATTGGGA 4981 AGACAATAGC AGGCATGCTG GGGACTCGAG TTAAGGGCGA ATTGCCGATT AGGATCTTCC 5041 TAGAGCATGG CTACGTAGAT AAGTAGCATG GCGGGTTAAT CATTAACTAC AAGGAACCCC 5101 TAGTGATGGA GTTGGCCACT CCCTCTCTGC GCGCTCGCTC GCTCACTGAG GCCGGGCGAC 5161 CAAAGGTCGC CCGACGCCCG GGCTTTGCCC GGGCGGCCTC AGTGAGCGAG CGAGCGCGCA 5221 GCCTTAATTA AGAGCTCATG GCGCGCCTAG GCCTTGACGG CCTTCCGCCA ATTCGCCCTA 5281 TAGTGAGTCG TATTACGTCG CGCTCACTGG CCGTCGTTTT ACAACGTCGT GACTGGGAAA 5341 ACCCTGGCGT TACCCAACTT AATCGCCTTG CAGCACATCC CCCTTTCGCC AGCTGGCGTA 5401 ATAGCGAAGA GGCCCGCACC GAAACGCCCT TCCCAACAGT TGCGCAGCCT GAATGGCGAA 5461 TGGGAGCGCC CTGTAGCGGC CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG 5521 GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC 5581 GAATTTTAAC AAAATATTAA CGCTTACAAT TTAG //