LOCUS GTDC1-pGEc2-DEST 6340 bp DNA circular 21-JAN-2013 FEATURES Location/Qualifiers misc_feature complement(5818..5947) /note="3'-ITR" misc_feature complement(5516..5730) /note="BGH pA" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" CDS 129..989 /dnas_title="AmpR" /label=AmpR /vntifkey="4" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_feature 4968..5509 /note="WPRE" misc_recomb complement(2974..3001) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_feature 4147..4169 /note="attB2" misc_feature 4947..4952 /note="BstB1 site" misc_feature 4170..4865 /note="Super GFP" misc_feature 4866..4916 /note="AviTag" misc_feature 4917..4940 /note="His Tag" PCR_frame 3002..4147 /note="Reading Frame" /set="Set 1" source 3006..4124 /dnas_title="GTDC1-pGEc2-DEST" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:21948 IMAGE:4390876" /tissue_type="Bone, osteosarcoma" /clone_lib="NIH_MGC_86" /lab_host="DH10B" /note="Vector: pCMV-SPORT6" gene 3006..4124 /dnas_title="GTDC1" /gene="GTDC1" /gene_synonym="mat-Xa" /db_xref="GeneID:79712" /db_xref="HGNC:20887" /db_xref="MIM:610165" CDS 3006..4124 /dnas_title="glycosyltransferase-like domain containing 1" /gene="GTDC1" /gene_synonym="mat-Xa" /codon_start=1 /product="glycosyltransferase-like domain containing 1" /protein_id="AAH17741.1" /db_xref="GI:34190921" /db_xref="GeneID:79712" /db_xref="HGNC:20887" /db_xref="MIM:610165" /translation="MSILIIEAFYGGSHKQLVDLLQEELGDCVVYTLPAKKWHWRART SALYFSQTIPISEHYRTLFASSVLNLTELAALRPDLGKLKKILYFHENQLIYPVKKCQ ERDFQYGYNQILSCLVADVVVFNSVFNMESFLTSMGKFMKLIPDHRPKDLESIIRPKC QVIYFPIRFPDVSRFMPKHKTTHLKKMLGLKGNGGAVLSMALPFQPEQRDSEDLLKNF NSECDTHCGLDTARQEYLGNSLRQESDLKKSTSSDNSSSHHGENKQNLTVDPCDILGG VDNQQRLLHIVWPHRWLEAVYCGCYPLCPKDLVYPEIFPAEYLYSTPEQLSKRLQNFC KRPDIIRKHLYKGEIAPFSWAALHGKFRSLLTTEPREDL" misc_feature 3006..4124 /note="GTDC1 (isoform 2) coding region " misc_feature 4125..4145 /note="TEV site" PCR_primer 2992..3001 /dnas_title="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /primer="CAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" PCR_primer complement(3002..4154) /dnas_title="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original="ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG" PCR_product complement(2992..4154) /pair="Pair 1" /set="Set 1" /current=1 /score="1.000" vector join(4155..6340,1..2991) /source="pGEc2-DEST" /type="Destination Vector" clone 2992..4154 /source="GTDC1-C-term-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCATCACAA GTTTGTACAA AAAAGCAGGC 3001 TCGCCatgag tatcctcatc attgaagcat tctatggagg ctcccataaa cagctggtgg 3061 atcttcttca agaagagtta ggagactgtg tcgtttatac ccttcctgca aagaaatggc 3121 attggagagc ccggacatct gctttatatt tctctcagac cattcccatc agtgagcatt 3181 acaggaccct ctttgcaagt tcagtgctta acctgaccga actggctgcc cttcggcctg 3241 accttgggaa actgaaaaag attctgtatt ttcacgagaa ccagttgata tatcctgtca 3301 agaaatgtca ggagagggat ttccaatatg gatacaacca aattctttca tgcctggtgg 3361 ctgatgtggt tgtattcaac tcagttttta atatggaatc atttctcact tccatgggaa 3421 aatttatgaa gctgattcct gatcacagac ccaaggatct ggaaagcatc atcagaccca 3481 agtgccaagt tatttacttt cccatcaggt ttcctgatgt gagcagattc atgcccaagc 3541 acaaaacaac ccatttaaag aagatgctcg gccttaaagg aaatggcggt gcggttctgt 3601 ccatggccct tccttttcag ccagagcaga gagattcaga ggatttattg aagaatttta 3661 attcagagtg tgatacacac tgtggccttg atactgcacg acaagaatat ttgggtaact 3721 cattaagaca ggaatcagac ttgaaaaaat ccacctcgtc agataattca agctctcatc 3781 atggtgaaaa taaacaaaat ctgactgttg atccctgtga cattttgggt ggagttgata 3841 atcagcaaag actgctacac attgtctggc ctcacaggtg gttggaagct gtgtactgtg 3901 ggtgttaccc actttgtcct aaagatttgg tttatcccga aatatttcca gctgaatatc 3961 tgtattctac acctgaacag ctttcaaaaa ggctccagaa tttctgcaag agaccagata 4021 ttataagaaa acatctctat aagggtgaaa tcgctccgtt ttcttgggca gccctacatg 4081 gtaaattcag gtctctgctt acaacagaac ctagggaaga tttggaaaac ttgtactttc 4141 aaggctCCAG CTTTCTTGTA CAAAGTGGTA TGAGCAAGGG CGAGGAACTG TTCACCGGCG 4201 TGGTGCCCAT CCTGGTGGAA CTGGATGGCG ACGTGAACGG CCACAAGTTC TCTGTGCGGG 4261 GAGAGGGCGA AGGCGACGCC ACAAATGGCA AGCTGACCCT GAAGTTCATC TGCACCACCG 4321 GCAAGCTGCC CGTGCCTTGG CCTACCCTCG TGACCACACT GACCTACGGC GTGCAGTGCT 4381 TCAGCAGATA CCCCGACCAT ATGAAGCGGC ACGACTTCTT CAAGAGCGCC ATGCCCGAGG 4441 GCTACGTGCA GGAACGGACC ATCAGCTTCA AGGACGACGG CACCTACAAG ACCAGAGCCG 4501 AAGTGAAGTT CGAGGGCGAC ACCCTCGTGA ACCGGATCGA GCTGAAGGGC ATCGATTTCA 4561 AAGAGGACGG CAACATCCTG GGCCACAAGC TGGAGTACAA CTTCAACAGC CACAACGTGT 4621 ACATCACCGC CGACAAGCAG AAGAACGGCA TCAAGGCCAA CTTCAAGATC CGGCACAACG 4681 TGGAAGATGG CAGCGTGCAG CTGGCCGACC ACTACCAGCA GAACACCCCC ATCGGAGATG 4741 GCCCCGTGCT GCTGCCCGAC AACCACTACC TGAGCACCCA GAGCGTGCTG AGCAAGGACC 4801 CCAACGAGAA GCGGGACCAC ATGGTGCTGC TGGAATTCGT GACCGCCGCT GGCATCACCC 4861 ACGGCATGAG CGGCCTGAAC GACATCTTCG AGGCCCAGAA AATCGAGTGG CACGAGCACC 4921 ACCATCATCA CCATCACCAC TGATGATTCG AAAAATAATA AGGATCCAAT CAACCTCTGG 4981 ATTACAAAAT TTGTGAAAGA TTGACTGGTA TTCTTAACTA TGTTGCTCCT TTTACGCTAT 5041 GTGGATACGC TGCTTTAATG CCTTTGTATC ATGCTATTGC TTCCCGTATG GCTTTCATTT 5101 TCTCCTCCTT GTATAAATCC TGGTTGCTGT CTCTTTATGA GGAGTTGTGG CCCGTTGTCA 5161 GGCAACGTGG CGTGGTGTGC ACTGTGTTTG CTGACGCAAC CCCCACTGGT TGGGGCATTG 5221 CCACCACCTG TCAGCTCCTT TCCGGGACTT TCGCTTTCCC CCTCCCTATT GCCACGGCGG 5281 AACTCATCGC CGCCTGCCTT GCCCGCTGCT GGACAGGGGC TCGGCTGTTG GGCACTGACA 5341 ATTCCGTGGT GTTGTCGGGG AAGCTGACGT CCTTTCCATG GCTGCTCGCC TGTGTTGCCA 5401 CCTGGATTCT GCGCGGGACG TCCTTCTGCT ACGTCCCTTC GGCCCTCAAT CCAGCGGACC 5461 TTCCTTCCCG CGGCCTGCTG CCGGCTCTGC GGCCTCTTCC GCGTCTTCGA GATCTGCCTC 5521 GACTGTGCCT TCTAGTTGCC AGCCATCTGT TGTTTGCCCC TCCCCCGTGC CTTCCTTGAC 5581 CCTGGAAGGT GCCACTCCCA CTGTCCTTTC CTAATAAAAT GAGGAAATTG CATCGCATTG 5641 TCTGAGTAGG TGTCATTCTA TTCTGGGGGG TGGGGTGGGG CAGGACAGCA AGGGGGAGGA 5701 TTGGGAAGAC AATAGCAGGC ATGCTGGGGA CTCGAGTTAA GGGCGAATTG CCGATTAGGA 5761 TCTTCCTAGA GCATGGCTAC GTAGATAAGT AGCATGGCGG GTTAATCATT AACTACAAGG 5821 AACCCCTAGT GATGGAGTTG GCCACTCCCT CTCTGCGCGC TCGCTCGCTC ACTGAGGCCG 5881 GGCGACCAAA GGTCGCCCGA CGCCCGGGCT TTGCCCGGGC GGCCTCAGTG AGCGAGCGAG 5941 CGCGCAGCCT TAATTAAGAG CTCATGGCGC GCCTAGGCCT TGACGGCCTT CCGCCAATTC 6001 GCCCTATAGT GAGTCGTATT ACGTCGCGCT CACTGGCCGT CGTTTTACAA CGTCGTGACT 6061 GGGAAAACCC TGGCGTTACC CAACTTAATC GCCTTGCAGC ACATCCCCCT TTCGCCAGCT 6121 GGCGTAATAG CGAAGAGGCC CGCACCGAAA CGCCCTTCCC AACAGTTGCG CAGCCTGAAT 6181 GGCGAATGGG AGCGCCCTGT AGCGGCCACT CAACCCTATC TCGGTCTATT CTTTTGATTT 6241 ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT 6301 TAACGCGAAT TTTAACAAAA TATTAACGCT TACAATTTAG //