LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2147..2243) /label=attL2 /note="attL2 (c)" misc_signal complement(2258..2277) /note="T7 Promoter/priming site (c)" primer_bind 2285..2301 /note="M13 Reverse priming site" gene 2414..3223 /note="Kanamycin resistance gene" rep_origin 3344..4017 /note="pUC origin" PCR_frame 669..2147 /note="Reading Frame" /set="Set 1" PCR_primer 652..668 /primer="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original=ACAAGTTTGTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC PCR_primer complement(669..2167) /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original=ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG PCR_product complement(652..2164) /pair="Pair 1" /set="Set 1" /current=1 /score=1.000 vector join(2155..4020,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 2125..2145 /note="TEV site" source 673..2124 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:46038 IMAGE:5733899" /tissue_type="Brain, hippocampus" /clone_lib="NIH_MGC_124" /lab_host="DH10B" /note="Vector: pCMV-SPORT6" gene 673..2124 /gene="PIGA" /gene_synonym=GPI3 /gene_synonym=PIG-A /db_xref="GeneID:5277" /db_xref="HGNC:8957" /db_xref="MIM:311770" CDS 673..2124 /dnas_title="phosphatidylinositol glycan anchor biosynthesis, class A" /gene="PIGA" /gene_synonym=GPI3 /gene_synonym=PIG-A /codon_start=1 /product="phosphatidylinositol glycan anchor biosynthesis, class A" /protein_id="AAH38236.1" /db_xref="GI:23398601" /db_xref="GeneID:5277" /db_xref="HGNC:8957" /db_xref="MIM:311770" /translation="MACRGGAGNGHRASATLSRVSPGSLYTCRTRTHNICMVSDFFYP NMGGVESHIYQLSQCLIERGHKVIIVTHAYGNRKGIRYLTSGLKVYYLPLKVMYNQST ATTLFHSLPLLRYIFVRERVTIIHSHSSFSAMAHDALFHAKTMGLQTVFTDHSLFGFA DVSSVLTNKLLTVSLCDTNHIICVSYTSKENTVLRAALNPEIVSVIPNAVDPTDFTPD PFRRHDSITIVVVSRLVYRKGIDLLSGIIPELCQKYPDLNFIIGGEGPKRIILEEVRE RYQLHDRVRLLGALEHKDVRNVLVQGHIFLNTSLTEAFCMAIVEAASCGLQVVSTRVG GIPEVLPENLIILCEPSVKSLCEGLEKAIFQLKSGTLPAPENIHNIVKTFYTWRNVAE RTEKVYDRVSVEAVLPMDKRLDRLISHCGPVTGYIFALLAVFNFLFLIFLRWMTPDSI IDVAIDATGPRGAWTNNYSHSKRGGENNEISETR" misc_feature 673..2124 /note="PIGA coding region" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTCG CCatggcctg tagaggagga gctgggaatg gccaccgtgc ctcagctaca 721 ctctctcggg ttagccctgg aagtctttac acatgtagaa cccgtaccca taatatatgc 781 atggtatctg actttttcta cccaaatatg ggaggcgtgg aaagccacat ttaccagctc 841 tctcagtgcc tgattgaaag agggcataag gttataattg tcacccatgc ttatggaaat 901 cgaaaaggca tccgttacct caccagtggc ctcaaagtct attacttgcc tctgaaagtc 961 atgtacaacc agtctacagc cacgaccctc tttcacagtc tgccattgct caggtacata 1021 tttgttcggg agagagtcac gataatccat tcacatagtt ctttttctgc tatggcccat 1081 gatgctctct tccacgccaa gacaatgggg cttcagacag tcttcacgga ccattccctt 1141 tttggatttg ctgatgtcag ctcggtgctt acaaacaagc ttctaaccgt gtctctttgt 1201 gatacaaacc acatcatttg tgtgtcttat actagtaagg aaaatactgt actaagagca 1261 gcactgaatc ctgaaatagt gtccgtcatt cctaatgctg tagatcctac tgacttcact 1321 ccagacccat ttagaaggca tgatagtata actattgttg ttgtcagcag acttgtttac 1381 agaaaaggga tcgatttgct tagtggtata atacctgaac tctgtcagaa atatccagat 1441 ttaaatttca taattggagg agagggacca aagagaatca ttttggaaga agttcgggaa 1501 agataccagc tgcatgacag ggtgcgtctt ttgggagctt tagaacacaa ggatgttaga 1561 aatgtcttag ttcaaggaca tatttttctg aatacctccc ttactgaagc attctgcatg 1621 gcgatcgtgg aagcagccag ttgtggttta caggttgtaa gtaccagagt tggtggaatt 1681 cctgaggtgc ttccagaaaa ccttattatt ttatgtgagc cttcagtaaa atctttgtgt 1741 gaaggattgg aaaaggctat tttccaactg aagtcaggga cattgccagc tccagaaaac 1801 atccataaca tagtaaagac tttctacacc tggaggaatg ttgcagaaag aactgaaaag 1861 gtatatgacc gggtatcagt ggaagctgtg ttgccaatgg acaaacgact ggacagactt 1921 atttctcact gcggcccagt aacaggctac atctttgctt tgttggcagt tttcaacttc 1981 ctcttcctca ttttcttgag atggatgact ccagattcta tcattgatgt tgcaatagat 2041 gccactgggc cacggggtgc ctggactaat aactattctc acagtaaaag agggggtgag 2101 aataatgaga tatctgaaac cagggaaaac ttgtactttc aaggctCCAG CTTTCTTGTA 2161 CAAAGTTGGC ATTATAAGAA AGCATTGCTT ATCAATTTGT TGCAACGAAC AGGTCACTAT 2221 CAGTCAAAAT AAAATCATTA TTTGCCATCC AGCTGATATC CCCTATAGTG AGTCGTATTA 2281 CATGGTCATA GCTGTTTCCT GGCAGCTCTG GCCCGTGTCT CAAAATCTCT GATGTTACAT 2341 TGCACAAGAT AAAATAATAT CATCATGAAC AATAAAACTG TCTGCTTACA TAAACAGTAA 2401 TACAAGGGGT GTTATGAGCC ATATTCAACG GGAAACGTCG AGGCCGCGAT TAAATTCCAA 2461 CATGGATGCT GATTTATATG GGTATAAATG GGCTCGCGAT AATGTCGGGC AATCAGGTGC 2521 GACAATCTAT CGCTTGTATG GGAAGCCCGA TGCGCCAGAG TTGTTTCTGA AACATGGCAA 2581 AGGTAGCGTT GCCAATGATG TTACAGATGA GATGGTCAGA CTAAACTGGC TGACGGAATT 2641 TATGCCTCTT CCGACCATCA AGCATTTTAT CCGTACTCCT GATGATGCAT GGTTACTCAC 2701 CACTGCGATC CCCGGAAAAA CAGCATTCCA GGTATTAGAA GAATATCCTG ATTCAGGTGA 2761 AAATATTGTT GATGCGCTGG CAGTGTTCCT GCGCCGGTTG CATTCGATTC CTGTTTGTAA 2821 TTGTCCTTTT AACAGCGATC GCGTATTTCG TCTCGCTCAG GCGCAATCAC GAATGAATAA 2881 CGGTTTGGTT GATGCGAGTG ATTTTGATGA CGAGCGTAAT GGCTGGCCTG TTGAACAAGT 2941 CTGGAAAGAA ATGCATAAAC TTTTGCCATT CTCACCGGAT TCAGTCGTCA CTCATGGTGA 3001 TTTCTCACTT GATAACCTTA TTTTTGACGA GGGGAAATTA ATAGGTTGTA TTGATGTTGG 3061 ACGAGTCGGA ATCGCAGACC GATACCAGGA TCTTGCCATC CTATGGAACT GCCTCGGTGA 3121 GTTTTCTCCT TCATTACAGA AACGGCTTTT TCAAAAATAT GGTATTGATA ATCCTGATAT 3181 GAATAAATTG CAGTTTCATT TGATGCTCGA TGAGTTTTTC TAATCAGAAT TGGTTAATTG 3241 GTTGTAACAC TGGCAGAGCA TTACGCTGAC TTGACGGGAC GGCGCAAGCT CATGACCAAA 3301 ATCCCTTAAC GTGAGTTACG CGTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA 3361 AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC 3421 ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT 3481 AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC CGTAGTTAGG 3541 CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC 3601 AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT 3661 ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA 3721 GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT 3781 TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG 3841 CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA 3901 CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA 3961 CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT //