LOCUS PIGA-pGEc2-DEST 6673 bp DNA circular 21-JAN-2013 FEATURES Location/Qualifiers misc_feature complement(6151..6280) /note="3'-ITR" misc_feature complement(5849..6063) /note="BGH pA" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" CDS 129..989 /dnas_title="AmpR" /label=AmpR /vntifkey="4" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_feature 5301..5842 /note="WPRE" misc_recomb complement(2974..3001) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_feature 4480..4502 /note="attB2" misc_feature 5280..5285 /note="BstB1 site" misc_feature 4503..5198 /note="Super GFP" misc_feature 5199..5249 /note="AviTag" misc_feature 5250..5273 /note="His Tag" PCR_frame 3002..4480 /note="Reading Frame" /set="Set 1" source 3006..4457 /dnas_title="PIGA-pGEc2-DEST" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:46038 IMAGE:5733899" /tissue_type="Brain, hippocampus" /clone_lib="NIH_MGC_124" /lab_host="DH10B" /note="Vector: pCMV-SPORT6" gene 3006..4457 /dnas_title="PIGA" /gene="PIGA" /gene_synonym="GPI3" /gene_synonym="PIG-A" /db_xref="GeneID:5277" /db_xref="HGNC:8957" /db_xref="MIM:311770" CDS 3006..4457 /dnas_title="phosphatidylinositol glycan anchor biosynthesis, class A" /gene="PIGA" /gene_synonym="GPI3" /gene_synonym="PIG-A" /codon_start=1 /product="phosphatidylinositol glycan anchor biosynthesis, class A" /protein_id="AAH38236.1" /db_xref="GI:23398601" /db_xref="GeneID:5277" /db_xref="HGNC:8957" /db_xref="MIM:311770" /translation="MACRGGAGNGHRASATLSRVSPGSLYTCRTRTHNICMVSDFFYP NMGGVESHIYQLSQCLIERGHKVIIVTHAYGNRKGIRYLTSGLKVYYLPLKVMYNQST ATTLFHSLPLLRYIFVRERVTIIHSHSSFSAMAHDALFHAKTMGLQTVFTDHSLFGFA DVSSVLTNKLLTVSLCDTNHIICVSYTSKENTVLRAALNPEIVSVIPNAVDPTDFTPD PFRRHDSITIVVVSRLVYRKGIDLLSGIIPELCQKYPDLNFIIGGEGPKRIILEEVRE RYQLHDRVRLLGALEHKDVRNVLVQGHIFLNTSLTEAFCMAIVEAASCGLQVVSTRVG GIPEVLPENLIILCEPSVKSLCEGLEKAIFQLKSGTLPAPENIHNIVKTFYTWRNVAE RTEKVYDRVSVEAVLPMDKRLDRLISHCGPVTGYIFALLAVFNFLFLIFLRWMTPDSI IDVAIDATGPRGAWTNNYSHSKRGGENNEISETR" misc_feature 3006..4457 /note="PIGA coding region" misc_feature 4458..4478 /note="TEV site" PCR_primer 2992..3001 /dnas_title="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /primer="CAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" PCR_primer complement(3002..4487) /dnas_title="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original="ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG" PCR_product complement(2992..4487) /pair="Pair 1" /set="Set 1" /current=1 /score="1.000" vector join(4488..6673,1..2991) /source="pGEc2-DEST" /type="Destination Vector" clone 2992..4487 /source="PIGA-C-term-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCATCACAA GTTTGTACAA AAAAGCAGGC 3001 TCGCCatggc ctgtagagga ggagctggga atggccaccg tgcctcagct acactctctc 3061 gggttagccc tggaagtctt tacacatgta gaacccgtac ccataatata tgcatggtat 3121 ctgacttttt ctacccaaat atgggaggcg tggaaagcca catttaccag ctctctcagt 3181 gcctgattga aagagggcat aaggttataa ttgtcaccca tgcttatgga aatcgaaaag 3241 gcatccgtta cctcaccagt ggcctcaaag tctattactt gcctctgaaa gtcatgtaca 3301 accagtctac agccacgacc ctctttcaca gtctgccatt gctcaggtac atatttgttc 3361 gggagagagt cacgataatc cattcacata gttctttttc tgctatggcc catgatgctc 3421 tcttccacgc caagacaatg gggcttcaga cagtcttcac ggaccattcc ctttttggat 3481 ttgctgatgt cagctcggtg cttacaaaca agcttctaac cgtgtctctt tgtgatacaa 3541 accacatcat ttgtgtgtct tatactagta aggaaaatac tgtactaaga gcagcactga 3601 atcctgaaat agtgtccgtc attcctaatg ctgtagatcc tactgacttc actccagacc 3661 catttagaag gcatgatagt ataactattg ttgttgtcag cagacttgtt tacagaaaag 3721 ggatcgattt gcttagtggt ataatacctg aactctgtca gaaatatcca gatttaaatt 3781 tcataattgg aggagaggga ccaaagagaa tcattttgga agaagttcgg gaaagatacc 3841 agctgcatga cagggtgcgt cttttgggag ctttagaaca caaggatgtt agaaatgtct 3901 tagttcaagg acatattttt ctgaatacct cccttactga agcattctgc atggcgatcg 3961 tggaagcagc cagttgtggt ttacaggttg taagtaccag agttggtgga attcctgagg 4021 tgcttccaga aaaccttatt attttatgtg agccttcagt aaaatctttg tgtgaaggat 4081 tggaaaaggc tattttccaa ctgaagtcag ggacattgcc agctccagaa aacatccata 4141 acatagtaaa gactttctac acctggagga atgttgcaga aagaactgaa aaggtatatg 4201 accgggtatc agtggaagct gtgttgccaa tggacaaacg actggacaga cttatttctc 4261 actgcggccc agtaacaggc tacatctttg ctttgttggc agttttcaac ttcctcttcc 4321 tcattttctt gagatggatg actccagatt ctatcattga tgttgcaata gatgccactg 4381 ggccacgggg tgcctggact aataactatt ctcacagtaa aagagggggt gagaataatg 4441 agatatctga aaccagggaa aacttgtact ttcaaggctC CAGCTTTCTT GTACAAAGTG 4501 GTATGAGCAA GGGCGAGGAA CTGTTCACCG GCGTGGTGCC CATCCTGGTG GAACTGGATG 4561 GCGACGTGAA CGGCCACAAG TTCTCTGTGC GGGGAGAGGG CGAAGGCGAC GCCACAAATG 4621 GCAAGCTGAC CCTGAAGTTC ATCTGCACCA CCGGCAAGCT GCCCGTGCCT TGGCCTACCC 4681 TCGTGACCAC ACTGACCTAC GGCGTGCAGT GCTTCAGCAG ATACCCCGAC CATATGAAGC 4741 GGCACGACTT CTTCAAGAGC GCCATGCCCG AGGGCTACGT GCAGGAACGG ACCATCAGCT 4801 TCAAGGACGA CGGCACCTAC AAGACCAGAG CCGAAGTGAA GTTCGAGGGC GACACCCTCG 4861 TGAACCGGAT CGAGCTGAAG GGCATCGATT TCAAAGAGGA CGGCAACATC CTGGGCCACA 4921 AGCTGGAGTA CAACTTCAAC AGCCACAACG TGTACATCAC CGCCGACAAG CAGAAGAACG 4981 GCATCAAGGC CAACTTCAAG ATCCGGCACA ACGTGGAAGA TGGCAGCGTG CAGCTGGCCG 5041 ACCACTACCA GCAGAACACC CCCATCGGAG ATGGCCCCGT GCTGCTGCCC GACAACCACT 5101 ACCTGAGCAC CCAGAGCGTG CTGAGCAAGG ACCCCAACGA GAAGCGGGAC CACATGGTGC 5161 TGCTGGAATT CGTGACCGCC GCTGGCATCA CCCACGGCAT GAGCGGCCTG AACGACATCT 5221 TCGAGGCCCA GAAAATCGAG TGGCACGAGC ACCACCATCA TCACCATCAC CACTGATGAT 5281 TCGAAAAATA ATAAGGATCC AATCAACCTC TGGATTACAA AATTTGTGAA AGATTGACTG 5341 GTATTCTTAA CTATGTTGCT CCTTTTACGC TATGTGGATA CGCTGCTTTA ATGCCTTTGT 5401 ATCATGCTAT TGCTTCCCGT ATGGCTTTCA TTTTCTCCTC CTTGTATAAA TCCTGGTTGC 5461 TGTCTCTTTA TGAGGAGTTG TGGCCCGTTG TCAGGCAACG TGGCGTGGTG TGCACTGTGT 5521 TTGCTGACGC AACCCCCACT GGTTGGGGCA TTGCCACCAC CTGTCAGCTC CTTTCCGGGA 5581 CTTTCGCTTT CCCCCTCCCT ATTGCCACGG CGGAACTCAT CGCCGCCTGC CTTGCCCGCT 5641 GCTGGACAGG GGCTCGGCTG TTGGGCACTG ACAATTCCGT GGTGTTGTCG GGGAAGCTGA 5701 CGTCCTTTCC ATGGCTGCTC GCCTGTGTTG CCACCTGGAT TCTGCGCGGG ACGTCCTTCT 5761 GCTACGTCCC TTCGGCCCTC AATCCAGCGG ACCTTCCTTC CCGCGGCCTG CTGCCGGCTC 5821 TGCGGCCTCT TCCGCGTCTT CGAGATCTGC CTCGACTGTG CCTTCTAGTT GCCAGCCATC 5881 TGTTGTTTGC CCCTCCCCCG TGCCTTCCTT GACCCTGGAA GGTGCCACTC CCACTGTCCT 5941 TTCCTAATAA AATGAGGAAA TTGCATCGCA TTGTCTGAGT AGGTGTCATT CTATTCTGGG 6001 GGGTGGGGTG GGGCAGGACA GCAAGGGGGA GGATTGGGAA GACAATAGCA GGCATGCTGG 6061 GGACTCGAGT TAAGGGCGAA TTGCCGATTA GGATCTTCCT AGAGCATGGC TACGTAGATA 6121 AGTAGCATGG CGGGTTAATC ATTAACTACA AGGAACCCCT AGTGATGGAG TTGGCCACTC 6181 CCTCTCTGCG CGCTCGCTCG CTCACTGAGG CCGGGCGACC AAAGGTCGCC CGACGCCCGG 6241 GCTTTGCCCG GGCGGCCTCA GTGAGCGAGC GAGCGCGCAG CCTTAATTAA GAGCTCATGG 6301 CGCGCCTAGG CCTTGACGGC CTTCCGCCAA TTCGCCCTAT AGTGAGTCGT ATTACGTCGC 6361 GCTCACTGGC CGTCGTTTTA CAACGTCGTG ACTGGGAAAA CCCTGGCGTT ACCCAACTTA 6421 ATCGCCTTGC AGCACATCCC CCTTTCGCCA GCTGGCGTAA TAGCGAAGAG GCCCGCACCG 6481 AAACGCCCTT CCCAACAGTT GCGCAGCCTG AATGGCGAAT GGGAGCGCCC TGTAGCGGCC 6541 ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA 6601 TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTTAACA AAATATTAAC 6661 GCTTACAATT TAG //