LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(1568..1664) /label=attL2 /note="attL2 (c)" misc_signal complement(1679..1698) /note="T7 Promoter/priming site (c)" primer_bind 1706..1722 /note="M13 Reverse priming site" gene 1835..2644 /note="Kanamycin resistance gene" rep_origin 2765..3438 /note="pUC origin" vector join(1576..3441,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 669..691 /note="TEV site" source 692..1561 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:97185 IMAGE:7262430" /tissue_type="PCR rescued clones" /clone_lib="NIH_MGC_277" /note="Vector: pPCR-Script Amp SK(+) with reversed insert" gene 692..1561 /gene="ABO" /gene_synonym=A3GALNT /gene_synonym=A3GALT1 /gene_synonym=GTB /gene_synonym=NAGAT /db_xref="GeneID:28" /db_xref="MIM:110300" CDS 692..1561 /dnas_title="ABO blood group (transferase A, alpha 1-3-N-acetylgalactosaminyltransferase; transferase B, alpha 1-3-galactosyltransferase)" /gene="ABO" /gene_synonym=A3GALNT /gene_synonym=A3GALT1 /gene_synonym=GTB /gene_synonym=NAGAT /codon_start=1 /product="ABO blood group (transferase A, alpha 1-3-N-acetylgalactosaminyltransferase; transferase B, alpha 1-3-galactosyltransferase)" /protein_id="AAH69595.1" /db_xref="GI:47479682" /db_xref="GeneID:28" /db_xref="MIM:110300" /translation="MAEVLRTLAGKPKCHALRPMILFLIMLVLVLFGYGVLSPRSLMP GSLERGFCMAVREPDHLQRVSLPRMVYPQPKVLTPCRKDVLVVTPWLAPIVWEGTFNI DILNEQFRLQNTTIGLTVFAIKKYVAFLKLFLETAEKHFMVGHRVHYYVFTDQPAAVP RVTLGTGRQLSVLEVGAYKRWQDVSMRRMEMISDFCERRFLSEVDYLVCVDVDMEFRD HVGVEILTPLFGTLHPSFYGSSREAFTYERRPQSQAYIPKDEGDFYYMGAFFGGSVQE VQRLTRACHQAMMVDQANGIEAVWHDESHLNKYLLRHKPTKVLSPEYLWDQQLLGWPA VLRKLRFTAVPKNHQAVRNP" misc_difference 719..719 /gene="ABO" /gene_synonym=A3GALNT /gene_synonym=A3GALT1 /gene_synonym=GTB /gene_synonym=NAGAT /note="'C' in cDNA is 'T' in the human genome; amino acid difference: 'P' in cDNA, 'S' in the human genome. The chimpanzee genome agrees with the cDNA sequence, suggesting that this difference is unlikely to be due to an artifact." misc_difference 760..760 /gene="ABO" /gene_synonym=A3GALNT /gene_synonym=A3GALT1 /gene_synonym=GTB /gene_synonym=NAGAT /note="1 base in cDNA is not found in the human genome. The chimpanzee genome agrees with the cDNA sequence, suggesting that this difference is unlikely to be due to an artifact." misc_difference 796..796 /gene="ABO" /gene_synonym=A3GALNT /gene_synonym=A3GALT1 /gene_synonym=GTB /gene_synonym=NAGAT /note="'G' in cDNA is 'A' in the human genome; no amino acid change. The chimpanzee genome agrees with the cDNA sequence, suggesting that this difference is unlikely to be due to an artifact." misc_difference 1025..1025 /gene="ABO" /gene_synonym=A3GALNT /gene_synonym=A3GALT1 /gene_synonym=GTB /gene_synonym=NAGAT /note="'G' in cDNA is 'C' in the human genome; amino acid difference: 'G' in cDNA, 'R' in the human genome. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." misc_difference 1156..1156 /gene="ABO" /gene_synonym=A3GALNT /gene_synonym=A3GALT1 /gene_synonym=GTB /gene_synonym=NAGAT /note="'T' in cDNA is 'C' in the human genome; no amino acid change. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." misc_feature 692..1561 /note="ABO coding region" misc_difference 1202..1202 /gene="ABO" /gene_synonym=A3GALNT /gene_synonym=A3GALT1 /gene_synonym=GTB /gene_synonym=NAGAT /note="'A' in cDNA is 'G' in the human genome; amino acid difference: 'S' in cDNA, 'G' in the human genome." misc_difference 1295..1295 /gene="ABO" /gene_synonym=A3GALNT /gene_synonym=A3GALT1 /gene_synonym=GTB /gene_synonym=NAGAT /note="'A' in cDNA is 'C' in the human genome; amino acid difference: 'M' in cDNA, 'L' in the human genome. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." misc_difference 1302..1302 /gene="ABO" /gene_synonym=A3GALNT /gene_synonym=A3GALT1 /gene_synonym=GTB /gene_synonym=NAGAT /note="'C' in cDNA is 'G' in the human genome; amino acid difference: 'A' in cDNA, 'G' in the human genome. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." misc_difference 1429..1429 /gene="ABO" /gene_synonym=A3GALNT /gene_synonym=A3GALT1 /gene_synonym=GTB /gene_synonym=NAGAT /note="'A' in cDNA is 'G' in the human genome; no amino acid change. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." misc_difference 1480..1480 /gene="ABO" /gene_synonym=A3GALNT /gene_synonym=A3GALT1 /gene_synonym=GTB /gene_synonym=NAGAT /note="'A' in cDNA is 'G' in the human genome; no amino acid change. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg ctcgttgcca aggatggtct acccccagcc 721 aaaggtgctg acaccgtgta ggaaggatgt cctcgtggtg accccttggc tggctcccat 781 tgtctgggag ggcacgttca acatcgacat cctcaacgag cagttcaggc tccagaacac 841 caccattggg ttaactgtgt ttgccatcaa gaaatacgtg gctttcctga agctgttcct 901 ggagacggcg gagaagcact tcatggtggg ccaccgtgtc cactactatg tcttcaccga 961 ccagccggcc gcggtgcccc gcgtgacgct ggggaccggt cggcagctgt cagtgctgga 1021 ggtgggcgcc tacaagcgct ggcaggacgt gtccatgcgc cgcatggaga tgatcagtga 1081 cttctgcgag cggcgcttcc tcagcgaggt ggattacctg gtgtgcgtgg acgtggacat 1141 ggagttccgc gaccatgtgg gcgtggagat cctgactccg ctgttcggca ccctgcaccc 1201 cagcttctac ggaagcagcc gggaggcctt cacctacgag cgccggcccc agtcccaggc 1261 ctacatcccc aaggacgagg gcgatttcta ctacatgggg gcgttcttcg gggggtcggt 1321 gcaagaggtg cagcggctca ccagggcctg ccaccaggcc atgatggtcg accaggccaa 1381 cggcatcgag gccgtgtggc acgacgagag ccacctgaac aagtacctac tgcgccacaa 1441 acccaccaag gtgctctccc ccgagtactt gtgggaccaa cagctgctgg gctggcccgc 1501 cgtcctgagg aagctgaggt tcactgcggt gcccaagaac caccaggcgg tccggaaccc 1561 gTAGGACCCA GCTTTCTTGT ACAAAGTTGG CATTATAAGA AAGCATTGCT TATCAATTTG 1621 TTGCAACGAA CAGGTCACTA TCAGTCAAAA TAAAATCATT ATTTGCCATC CAGCTGATAT 1681 CCCCTATAGT GAGTCGTATT ACATGGTCAT AGCTGTTTCC TGGCAGCTCT GGCCCGTGTC 1741 TCAAAATCTC TGATGTTACA TTGCACAAGA TAAAATAATA TCATCATGAA CAATAAAACT 1801 GTCTGCTTAC ATAAACAGTA ATACAAGGGG TGTTATGAGC CATATTCAAC GGGAAACGTC 1861 GAGGCCGCGA TTAAATTCCA ACATGGATGC TGATTTATAT GGGTATAAAT GGGCTCGCGA 1921 TAATGTCGGG CAATCAGGTG CGACAATCTA TCGCTTGTAT GGGAAGCCCG ATGCGCCAGA 1981 GTTGTTTCTG AAACATGGCA AAGGTAGCGT TGCCAATGAT GTTACAGATG AGATGGTCAG 2041 ACTAAACTGG CTGACGGAAT TTATGCCTCT TCCGACCATC AAGCATTTTA TCCGTACTCC 2101 TGATGATGCA TGGTTACTCA CCACTGCGAT CCCCGGAAAA ACAGCATTCC AGGTATTAGA 2161 AGAATATCCT GATTCAGGTG AAAATATTGT TGATGCGCTG GCAGTGTTCC TGCGCCGGTT 2221 GCATTCGATT CCTGTTTGTA ATTGTCCTTT TAACAGCGAT CGCGTATTTC GTCTCGCTCA 2281 GGCGCAATCA CGAATGAATA ACGGTTTGGT TGATGCGAGT GATTTTGATG ACGAGCGTAA 2341 TGGCTGGCCT GTTGAACAAG TCTGGAAAGA AATGCATAAA CTTTTGCCAT TCTCACCGGA 2401 TTCAGTCGTC ACTCATGGTG ATTTCTCACT TGATAACCTT ATTTTTGACG AGGGGAAATT 2461 AATAGGTTGT ATTGATGTTG GACGAGTCGG AATCGCAGAC CGATACCAGG ATCTTGCCAT 2521 CCTATGGAAC TGCCTCGGTG AGTTTTCTCC TTCATTACAG AAACGGCTTT TTCAAAAATA 2581 TGGTATTGAT AATCCTGATA TGAATAAATT GCAGTTTCAT TTGATGCTCG ATGAGTTTTT 2641 CTAATCAGAA TTGGTTAATT GGTTGTAACA CTGGCAGAGC ATTACGCTGA CTTGACGGGA 2701 CGGCGCAAGC TCATGACCAA AATCCCTTAA CGTGAGTTAC GCGTCGTTCC ACTGAGCGTC 2761 AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG 2821 CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT 2881 ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTTCT 2941 TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT 3001 CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG 3061 GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC 3121 GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA 3181 GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG 3241 CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA 3301 TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG 3361 GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG 3421 CTGGCCTTTT GCTCACATGT T //