LOCUS ABO-pGEn1-DEST 5452 bp DNA circular 21-JAN-2013 FEATURES Location/Qualifiers CDS 129..1108 /dnas_title="AmpR" /label=AmpR /vntifkey="4" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_feature 2977..3054 /note="TCM Sig Seq" misc_recomb complement(3114..3142) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_feature 3055..3078 /note="8xHis Tag" misc_feature 3085..3108 /note="StrepTag" misc_feature 4080..4621 /note="WPRE" misc_recomb 4042..4064 /label=attB2 /note="attB2 site" /note="recognized as attB2" misc_feature complement(4628..4842) /note="BGH pA" misc_feature complement(4930..5059) /note="3'-ITR" misc_feature 3143..3165 /note="TEV site" source 3166..4035 /dnas_title="ABO-pGEn1-DEST" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:97185 IMAGE:7262430" /tissue_type="PCR rescued clones" /clone_lib="NIH_MGC_277" /note="Vector: pPCR-Script Amp SK(+) with reversed insert" gene 3166..4035 /dnas_title="ABO" /gene="ABO" /gene_synonym="A3GALNT" /gene_synonym="A3GALT1" /gene_synonym="GTB" /gene_synonym="NAGAT" /db_xref="GeneID:28" /db_xref="MIM:110300" CDS 3166..4035 /dnas_title="ABO blood group (transferase A, alpha 1-3-N-acetylgalactosaminyltransferase; transferase B, alpha 1-3-galactosyltransferase)" /gene="ABO" /gene_synonym="A3GALNT" /gene_synonym="A3GALT1" /gene_synonym="GTB" /gene_synonym="NAGAT" /codon_start=1 /product="ABO blood group (transferase A, alpha 1-3-N-acetylgalactosaminyltransferase; transferase B, alpha 1-3-galactosyltransferase)" /protein_id="AAH69595.1" /db_xref="GI:47479682" /db_xref="GeneID:28" /db_xref="MIM:110300" /translation="MAEVLRTLAGKPKCHALRPMILFLIMLVLVLFGYGVLSPRSLMP GSLERGFCMAVREPDHLQRVSLPRMVYPQPKVLTPCRKDVLVVTPWLAPIVWEGTFNI DILNEQFRLQNTTIGLTVFAIKKYVAFLKLFLETAEKHFMVGHRVHYYVFTDQPAAVP RVTLGTGRQLSVLEVGAYKRWQDVSMRRMEMISDFCERRFLSEVDYLVCVDVDMEFRD HVGVEILTPLFGTLHPSFYGSSREAFTYERRPQSQAYIPKDEGDFYYMGAFFGGSVQE VQRLTRACHQAMMVDQANGIEAVWHDESHLNKYLLRHKPTKVLSPEYLWDQQLLGWPA VLRKLRFTAVPKNHQAVRNP" misc_difference 3193..3193 /dnas_title="ABO" /gene="ABO" /gene_synonym="A3GALNT" /gene_synonym="A3GALT1" /gene_synonym="GTB" /gene_synonym="NAGAT" /note="'C' in cDNA is 'T' in the human genome; amino acid difference: 'P' in cDNA, 'S' in the human genome. The chimpanzee genome agrees with the cDNA sequence, suggesting that this difference is unlikely to be due to an artifact." misc_difference 3234..3234 /dnas_title="ABO" /gene="ABO" /gene_synonym="A3GALNT" /gene_synonym="A3GALT1" /gene_synonym="GTB" /gene_synonym="NAGAT" /note="1 base in cDNA is not found in the human genome. The chimpanzee genome agrees with the cDNA sequence, suggesting that this difference is unlikely to be due to an artifact." misc_difference 3270..3270 /dnas_title="ABO" /gene="ABO" /gene_synonym="A3GALNT" /gene_synonym="A3GALT1" /gene_synonym="GTB" /gene_synonym="NAGAT" /note="'G' in cDNA is 'A' in the human genome; no amino acid change. The chimpanzee genome agrees with the cDNA sequence, suggesting that this difference is unlikely to be due to an artifact." misc_feature 3166..4035 /note="ABO coding region" misc_difference 3499..3499 /dnas_title="ABO" /gene="ABO" /gene_synonym="A3GALNT" /gene_synonym="A3GALT1" /gene_synonym="GTB" /gene_synonym="NAGAT" /note="'G' in cDNA is 'C' in the human genome; amino acid difference: 'G' in cDNA, 'R' in the human genome. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." misc_difference 3630..3630 /dnas_title="ABO" /gene="ABO" /gene_synonym="A3GALNT" /gene_synonym="A3GALT1" /gene_synonym="GTB" /gene_synonym="NAGAT" /note="'T' in cDNA is 'C' in the human genome; no amino acid change. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." misc_difference 3676..3676 /dnas_title="ABO" /gene="ABO" /gene_synonym="A3GALNT" /gene_synonym="A3GALT1" /gene_synonym="GTB" /gene_synonym="NAGAT" /note="'A' in cDNA is 'G' in the human genome; amino acid difference: 'S' in cDNA, 'G' in the human genome." misc_difference 3769..3769 /dnas_title="ABO" /gene="ABO" /gene_synonym="A3GALNT" /gene_synonym="A3GALT1" /gene_synonym="GTB" /gene_synonym="NAGAT" /note="'A' in cDNA is 'C' in the human genome; amino acid difference: 'M' in cDNA, 'L' in the human genome. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." misc_difference 3776..3776 /dnas_title="ABO" /gene="ABO" /gene_synonym="A3GALNT" /gene_synonym="A3GALT1" /gene_synonym="GTB" /gene_synonym="NAGAT" /note="'C' in cDNA is 'G' in the human genome; amino acid difference: 'A' in cDNA, 'G' in the human genome. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." misc_difference 3903..3903 /dnas_title="ABO" /gene="ABO" /gene_synonym="A3GALNT" /gene_synonym="A3GALT1" /gene_synonym="GTB" /gene_synonym="NAGAT" /note="'A' in cDNA is 'G' in the human genome; no amino acid change. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." misc_difference 3954..3954 /dnas_title="ABO" /gene="ABO" /gene_synonym="A3GALNT" /gene_synonym="A3GALT1" /gene_synonym="GTB" /gene_synonym="NAGAT" /note="'A' in cDNA is 'G' in the human genome; no amino acid change. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." vector join(4050..5452,1..3132) /source="pGEn1-DEST" /type="Destination Vector" clone 3133..4049 /source="ABO-Nterm-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCGCCATGA GACTGCTGAC AGCCCTGTTC 3001 GCCTACTTCA TCGTGGCCCT GATCCTGGCC TTCAGCGTGT CCGCCAAGAG CATGCACCAC 3061 CACCATCACC ATCATCACAG CGCCTGGTCC CACCCCCAGT TCGAGAAAGA ATTATCAACA 3121 AGTTTGTACA AAAAAGCAGG CTctgaaaac ttgtactttc aaggctcgtt gccaaggatg 3181 gtctaccccc agccaaaggt gctgacaccg tgtaggaagg atgtcctcgt ggtgacccct 3241 tggctggctc ccattgtctg ggagggcacg ttcaacatcg acatcctcaa cgagcagttc 3301 aggctccaga acaccaccat tgggttaact gtgtttgcca tcaagaaata cgtggctttc 3361 ctgaagctgt tcctggagac ggcggagaag cacttcatgg tgggccaccg tgtccactac 3421 tatgtcttca ccgaccagcc ggccgcggtg ccccgcgtga cgctggggac cggtcggcag 3481 ctgtcagtgc tggaggtggg cgcctacaag cgctggcagg acgtgtccat gcgccgcatg 3541 gagatgatca gtgacttctg cgagcggcgc ttcctcagcg aggtggatta cctggtgtgc 3601 gtggacgtgg acatggagtt ccgcgaccat gtgggcgtgg agatcctgac tccgctgttc 3661 ggcaccctgc accccagctt ctacggaagc agccgggagg ccttcaccta cgagcgccgg 3721 ccccagtccc aggcctacat ccccaaggac gagggcgatt tctactacat gggggcgttc 3781 ttcggggggt cggtgcaaga ggtgcagcgg ctcaccaggg cctgccacca ggccatgatg 3841 gtcgaccagg ccaacggcat cgaggccgtg tggcacgacg agagccacct gaacaagtac 3901 ctactgcgcc acaaacccac caaggtgctc tcccccgagt acttgtggga ccaacagctg 3961 ctgggctggc ccgccgtcct gaggaagctg aggttcactg cggtgcccaa gaaccaccag 4021 gcggtccgga acccgTAGGA CCCAGCTTTC TTGTACAAAG TGGTTGATAG CTTGGATCCA 4081 ATCAACCTCT GGATTACAAA ATTTGTGAAA GATTGACTGG TATTCTTAAC TATGTTGCTC 4141 CTTTTACGCT ATGTGGATAC GCTGCTTTAA TGCCTTTGTA TCATGCTATT GCTTCCCGTA 4201 TGGCTTTCAT TTTCTCCTCC TTGTATAAAT CCTGGTTGCT GTCTCTTTAT GAGGAGTTGT 4261 GGCCCGTTGT CAGGCAACGT GGCGTGGTGT GCACTGTGTT TGCTGACGCA ACCCCCACTG 4321 GTTGGGGCAT TGCCACCACC TGTCAGCTCC TTTCCGGGAC TTTCGCTTTC CCCCTCCCTA 4381 TTGCCACGGC GGAACTCATC GCCGCCTGCC TTGCCCGCTG CTGGACAGGG GCTCGGCTGT 4441 TGGGCACTGA CAATTCCGTG GTGTTGTCGG GGAAGCTGAC GTCCTTTCCA TGGCTGCTCG 4501 CCTGTGTTGC CACCTGGATT CTGCGCGGGA CGTCCTTCTG CTACGTCCCT TCGGCCCTCA 4561 ATCCAGCGGA CCTTCCTTCC CGCGGCCTGC TGCCGGCTCT GCGGCCTCTT CCGCGTCTTC 4621 GAGATCTGCC TCGACTGTGC CTTCTAGTTG CCAGCCATCT GTTGTTTGCC CCTCCCCCGT 4681 GCCTTCCTTG ACCCTGGAAG GTGCCACTCC CACTGTCCTT TCCTAATAAA ATGAGGAAAT 4741 TGCATCGCAT TGTCTGAGTA GGTGTCATTC TATTCTGGGG GGTGGGGTGG GGCAGGACAG 4801 CAAGGGGGAG GATTGGGAAG ACAATAGCAG GCATGCTGGG GACTCGAGTT AAGGGCGAAT 4861 TGCCGATTAG GATCTTCCTA GAGCATGGCT ACGTAGATAA GTAGCATGGC GGGTTAATCA 4921 TTAACTACAA GGAACCCCTA GTGATGGAGT TGGCCACTCC CTCTCTGCGC GCTCGCTCGC 4981 TCACTGAGGC CGGGCGACCA AAGGTCGCCC GACGCCCGGG CTTTGCCCGG GCGGCCTCAG 5041 TGAGCGAGCG AGCGCGCAGC CTTAATTAAG AGCTCATGGC GCGCCTAGGC CTTGACGGCC 5101 TTCCGCCAAT TCGCCCTATA GTGAGTCGTA TTACGTCGCG CTCACTGGCC GTCGTTTTAC 5161 AACGTCGTGA CTGGGAAAAC CCTGGCGTTA CCCAACTTAA TCGCCTTGCA GCACATCCCC 5221 CTTTCGCCAG CTGGCGTAAT AGCGAAGAGG CCCGCACCGA AACGCCCTTC CCAACAGTTG 5281 CGCAGCCTGA ATGGCGAATG GGAGCGCCCT GTAGCGGCCA CTCAACCCTA TCTCGGTCTA 5341 TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT 5401 TTAACAAAAA TTTAACGCGA ATTTTAACAA AATATTAACG CTTACAATTT AG //