LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(1745..1841) /label=attL2 /note="attL2 (c)" misc_signal complement(1856..1875) /note="T7 Promoter/priming site (c)" primer_bind 1883..1899 /note="M13 Reverse priming site" gene 2012..2821 /note="Kanamycin resistance gene" rep_origin 2942..3615 /note="pUC origin" vector join(1753..3618,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 669..691 /note="TEV site" source 692..1738 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:2007 IMAGE:3357342" /tissue_type="Eye, retinoblastoma" /clone_lib="NIH_MGC_16" /lab_host="DH10B-R" /note="Vector: pOTB7" gene 692..1738 /gene="B4GALT3" /gene_synonym=beta4Gal-T3 /db_xref="GeneID:8703" /db_xref="HGNC:926" /db_xref="MIM:604014" CDS 692..1738 /dnas_title="UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 3" /gene="B4GALT3" /gene_synonym=beta4Gal-T3 /codon_start=1 /product="UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 3" /protein_id="AAH00276.1" /db_xref="GI:12653027" /db_xref="GeneID:8703" /db_xref="HGNC:926" /db_xref="MIM:604014" /translation="MLRRLLERPCTLALLVGSQLAVMMYLSLGGFRSLSALFGRDQGP TFDYSHPRDVYSNLSHLPGAPGGPPAPQGLPYCPERSPLLVGPVSVSFSPVPSLAEIV ERNPRVEPGGRYRPAGCEPRSRTAIIVPHRAREHHLRLLLYHLHPFLQRQQLAYGIYV IHQAGNGTFNRAKLLNVGVREALRDEEWDCLFLHDVDLLPENDHNLYVCDPRGPRHVA VAMNKFGYSLPYPQYFGGVSALTPDQYLKMNGFPNEYWGWGGEDDDIATRVRLAGMKI SRPPTSVGHYKMVKHRGDKGNEENPHRFDLLVRTQNSWTQDGMNSLTYQLLARELGPL YTNITADIGTDPRGPRAPSGPRYPPGSSQAFRQEMLQRRPPARPGPLSTANHTALRGS H" misc_difference 826..826 /gene="B4GALT3" /gene_synonym=beta4Gal-T3 /note="'A' in cDNA is 'G' in the human genome; no amino acid change. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." misc_difference 1087..1087 /gene="B4GALT3" /gene_synonym=beta4Gal-T3 /note="'T' in cDNA is 'C' in the human genome; no amino acid change. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." misc_feature 692..1738 /note="B4GALT3 coding region" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg cacatttgac tattctcacc ctcgtgatgt 721 ctacagtaac ctcagtcacc tgcctggggc cccagggggt cctccagctc ctcaaggtct 781 gccctactgt ccagaacgat ctcctctctt agtgggtcct gtgtcagtgt cctttagccc 841 agtgccatca ctggcagaga ttgtggagcg gaatccccgg gtagaaccag ggggccggta 901 ccgccctgca ggttgtgagc cccgctcccg aacagccatc attgtgcctc atcgtgcccg 961 ggagcaccac ctgcgcctgc tgctctacca cctgcacccc ttcttgcagc gccagcagct 1021 tgcttatggc atctatgtca tccaccaggc tggaaatgga acatttaaca gggcaaaact 1081 gttgaatgtt ggggtgcgag aggccctgcg tgatgaagag tgggactgcc tgttcttgca 1141 cgatgtggac ctcttgccag aaaatgacca caatctgtat gtgtgtgacc cccggggacc 1201 ccgccatgtt gccgttgcta tgaacaagtt tggatacagc ctcccgtacc cccagtactt 1261 cggaggagtc tcagcactta ctcctgacca gtacctgaag atgaatggct tccccaatga 1321 atactggggc tggggtggtg aggatgacga cattgctacc agggtgcgcc tggctgggat 1381 gaagatctct cggcccccca catctgtagg acactataag atggtgaagc accgaggaga 1441 taagggcaat gaggaaaatc cccacagatt tgacctcctg gtccgtaccc agaattcctg 1501 gacgcaagat gggatgaact cactgacata ccagttgctg gctcgagagc tggggcctct 1561 ttataccaac atcacagcag acattgggac tgaccctcgg ggtcctcggg ctccttctgg 1621 gccacgttac ccacctggtt cctcccaagc cttccgtcaa gagatgctgc aacgccggcc 1681 cccagccagg cctgggcctc tatctactgc caaccacaca gccctccgag gttcacacTA 1741 GGACCCAGCT TTCTTGTACA AAGTTGGCAT TATAAGAAAG CATTGCTTAT CAATTTGTTG 1801 CAACGAACAG GTCACTATCA GTCAAAATAA AATCATTATT TGCCATCCAG CTGATATCCC 1861 CTATAGTGAG TCGTATTACA TGGTCATAGC TGTTTCCTGG CAGCTCTGGC CCGTGTCTCA 1921 AAATCTCTGA TGTTACATTG CACAAGATAA AATAATATCA TCATGAACAA TAAAACTGTC 1981 TGCTTACATA AACAGTAATA CAAGGGGTGT TATGAGCCAT ATTCAACGGG AAACGTCGAG 2041 GCCGCGATTA AATTCCAACA TGGATGCTGA TTTATATGGG TATAAATGGG CTCGCGATAA 2101 TGTCGGGCAA TCAGGTGCGA CAATCTATCG CTTGTATGGG AAGCCCGATG CGCCAGAGTT 2161 GTTTCTGAAA CATGGCAAAG GTAGCGTTGC CAATGATGTT ACAGATGAGA TGGTCAGACT 2221 AAACTGGCTG ACGGAATTTA TGCCTCTTCC GACCATCAAG CATTTTATCC GTACTCCTGA 2281 TGATGCATGG TTACTCACCA CTGCGATCCC CGGAAAAACA GCATTCCAGG TATTAGAAGA 2341 ATATCCTGAT TCAGGTGAAA ATATTGTTGA TGCGCTGGCA GTGTTCCTGC GCCGGTTGCA 2401 TTCGATTCCT GTTTGTAATT GTCCTTTTAA CAGCGATCGC GTATTTCGTC TCGCTCAGGC 2461 GCAATCACGA ATGAATAACG GTTTGGTTGA TGCGAGTGAT TTTGATGACG AGCGTAATGG 2521 CTGGCCTGTT GAACAAGTCT GGAAAGAAAT GCATAAACTT TTGCCATTCT CACCGGATTC 2581 AGTCGTCACT CATGGTGATT TCTCACTTGA TAACCTTATT TTTGACGAGG GGAAATTAAT 2641 AGGTTGTATT GATGTTGGAC GAGTCGGAAT CGCAGACCGA TACCAGGATC TTGCCATCCT 2701 ATGGAACTGC CTCGGTGAGT TTTCTCCTTC ATTACAGAAA CGGCTTTTTC AAAAATATGG 2761 TATTGATAAT CCTGATATGA ATAAATTGCA GTTTCATTTG ATGCTCGATG AGTTTTTCTA 2821 ATCAGAATTG GTTAATTGGT TGTAACACTG GCAGAGCATT ACGCTGACTT GACGGGACGG 2881 CGCAAGCTCA TGACCAAAAT CCCTTAACGT GAGTTACGCG TCGTTCCACT GAGCGTCAGA 2941 CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG 3001 CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC 3061 AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTTCTTCT 3121 AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC 3181 TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT 3241 GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG 3301 CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT 3361 ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG 3421 GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG 3481 TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG 3541 GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG 3601 GCCTTTTGCT CACATGTT //