LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(1982..2078) /label=attL2 /note="attL2 (c)" misc_signal complement(2093..2112) /note="T7 Promoter/priming site (c)" primer_bind 2120..2136 /note="M13 Reverse priming site" gene 2249..3058 /note="Kanamycin resistance gene" rep_origin 3179..3852 /note="pUC origin" vector join(1990..3855,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 669..691 /note="TEV site" source 692..1975 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:9153 IMAGE:3923041" /tissue_type="Skin, melanotic melanoma." /clone_lib="NIH_MGC_72" /lab_host="DH10B" /note="Vector: pCMV-SPORT6" gene 692..1975 /gene="GYG2" /gene_synonym=GN-2 /gene_synonym=GN2 /db_xref="GeneID:8908" /db_xref="HGNC:4700" /db_xref="MIM:300198" CDS 692..1975 /dnas_title="glycogenin 2" /gene="GYG2" /gene_synonym=GN-2 /gene_synonym=GN2 /codon_start=1 /product="glycogenin 2" /protein_id="AAH23152.1" /db_xref="GI:18605503" /db_xref="GeneID:8908" /db_xref="HGNC:4700" /db_xref="MIM:300198" /translation="MSVTDQAFVTLATNDIYCQGALVLGQSLRRHRLTRKLVVLITPQ VSSLLRVILSKVFDEVIEVNLIDSADYIHLAFLKRPELGLTLTKLHCWTLTHYSKCVF LDADTLVLSNVDELFDRGEFSAAPDPGWPDCFNSGVFVFQPSLHTHKLLLQHAMEHGS FDGADQGLLNSFFRNWSTTDIHKHLPFIYNLSSNTMYTYSPAFKQFGSSAKVVHFLGS MKPWNYKYNPQSGSVLEQGSVSSSQHQAAFLHLWWTVYQNNVLPLYKSVQAGEARASP GHTLCRSDVGGPCADSASGVGEPCENSTPSAGVPCANSPLGSNQPAQGLPEPTQIVDE TLSLPEGRRSEDMIACPETETPAVITCDPLSQPSPQPADFTETETILQPANKVESVSS EETFEPSQELPAEALRDPSLQDALEVDLAVSVSQISIEEKVKELSPEEERRKWEEGRI DYMGKDAFARIQEKLDRFLQ" misc_feature 692..1975 /note="GYG2 coding region" misc_difference 1281..1281 /gene="GYG2" /gene_synonym=GN-2 /gene_synonym=GN2 /note="'T' in cDNA is 'C' in the human genome; amino acid difference: 'V' in cDNA, 'A' in the human genome. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." misc_difference 1410..1410 /gene="GYG2" /gene_synonym=GN-2 /gene_synonym=GN2 /note="'G' in cDNA is 'A' in the human genome; amino acid difference: 'R' in cDNA, 'H' in the human genome." ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg ccctcaggtg tccagcctgc tcagggtcat 721 cctctcgaag gtgttcgatg aagtcattga agtgaatcta atcgatagtg ccgactacat 781 ccacctggcc tttctgaaga gacctgagct cgggctcacc ctcaccaagc ttcactgttg 841 gactctcact cactacagca agtgtgtctt cctggatgca gacactctgg tgctgtccaa 901 tgtcgatgag ctgtttgaca ggggagagtt ttctgcggcc ccggaccccg gatggccgga 961 ttgcttcaat agcggggtgt ttgtcttcca gccttctctc cacacgcata aactcctgct 1021 acagcacgcc atggaacacg gcagctttga cggggcagac caaggcttac tgaatagttt 1081 cttcaggaac tggtcgacca cagacatcca caagcacctg ccgttcatct ataacttgag 1141 tagtaacacg atgtacactt acagccctgc cttcaagcaa ttcggttcca gtgcaaaggt 1201 cgtccacttt ttggggtcca tgaaaccttg gaactacaag tacaatccac agagtggctc 1261 ggtgttggag caaggctcag tgtccagcag ccagcaccag gcggcattcc ttcatctctg 1321 gtggacggtc taccagaaca acgtgctgcc cctttataaa agcgtccaag cgggggaagc 1381 acgcgcgtct cctggtcaca cactttgccg cagtgatgtg ggggggccgt gtgcggattc 1441 agcctctggt gttggagagc cgtgtgaaaa ttcaacaccc agtgcgggcg tgccgtgtgc 1501 aaattcacca ctgggttcta accagcctgc tcagggcctt ccggagccga cccagatagt 1561 ggatgagacc ctgtccctac ctgaaggacg ccgttcagaa gatatgatag cttgtcctga 1621 aactgagact cctgccgtga taacgtgtga cccactgtcc cagccttccc ctcagcctgc 1681 agacttcaca gagactgaaa ccatcttgca gccagcaaat aaagtcgaaa gtgtctcatc 1741 cgaggaaacc ttcgaaccaa gccaggaact ccctgctgag gctctcaggg accccagtct 1801 gcaggatgca ctggaggtcg acctggccgt ctctgtttcc cagatctcca tcgaagagaa 1861 ggtgaaggaa ttgagccccg aggaagagag gaggaagtgg gaggaaggcc gtatcgacta 1921 catggggaag gacgcgtttg ctcgcatcca ggagaagctg gaccggttcc tgcagTAGGA 1981 CCCAGCTTTC TTGTACAAAG TTGGCATTAT AAGAAAGCAT TGCTTATCAA TTTGTTGCAA 2041 CGAACAGGTC ACTATCAGTC AAAATAAAAT CATTATTTGC CATCCAGCTG ATATCCCCTA 2101 TAGTGAGTCG TATTACATGG TCATAGCTGT TTCCTGGCAG CTCTGGCCCG TGTCTCAAAA 2161 TCTCTGATGT TACATTGCAC AAGATAAAAT AATATCATCA TGAACAATAA AACTGTCTGC 2221 TTACATAAAC AGTAATACAA GGGGTGTTAT GAGCCATATT CAACGGGAAA CGTCGAGGCC 2281 GCGATTAAAT TCCAACATGG ATGCTGATTT ATATGGGTAT AAATGGGCTC GCGATAATGT 2341 CGGGCAATCA GGTGCGACAA TCTATCGCTT GTATGGGAAG CCCGATGCGC CAGAGTTGTT 2401 TCTGAAACAT GGCAAAGGTA GCGTTGCCAA TGATGTTACA GATGAGATGG TCAGACTAAA 2461 CTGGCTGACG GAATTTATGC CTCTTCCGAC CATCAAGCAT TTTATCCGTA CTCCTGATGA 2521 TGCATGGTTA CTCACCACTG CGATCCCCGG AAAAACAGCA TTCCAGGTAT TAGAAGAATA 2581 TCCTGATTCA GGTGAAAATA TTGTTGATGC GCTGGCAGTG TTCCTGCGCC GGTTGCATTC 2641 GATTCCTGTT TGTAATTGTC CTTTTAACAG CGATCGCGTA TTTCGTCTCG CTCAGGCGCA 2701 ATCACGAATG AATAACGGTT TGGTTGATGC GAGTGATTTT GATGACGAGC GTAATGGCTG 2761 GCCTGTTGAA CAAGTCTGGA AAGAAATGCA TAAACTTTTG CCATTCTCAC CGGATTCAGT 2821 CGTCACTCAT GGTGATTTCT CACTTGATAA CCTTATTTTT GACGAGGGGA AATTAATAGG 2881 TTGTATTGAT GTTGGACGAG TCGGAATCGC AGACCGATAC CAGGATCTTG CCATCCTATG 2941 GAACTGCCTC GGTGAGTTTT CTCCTTCATT ACAGAAACGG CTTTTTCAAA AATATGGTAT 3001 TGATAATCCT GATATGAATA AATTGCAGTT TCATTTGATG CTCGATGAGT TTTTCTAATC 3061 AGAATTGGTT AATTGGTTGT AACACTGGCA GAGCATTACG CTGACTTGAC GGGACGGCGC 3121 AAGCTCATGA CCAAAATCCC TTAACGTGAG TTACGCGTCG TTCCACTGAG CGTCAGACCC 3181 CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT 3241 GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC 3301 TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TTCTTCTAGT 3361 GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT 3421 GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA 3481 CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC 3541 ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG 3601 AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT 3661 CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC 3721 TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG 3781 GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC 3841 TTTTGCTCAC ATGTT //