LOCUS LARGE-pGEn1-DEST 6580 bp DNA circular 21-JAN-2013 FEATURES Location/Qualifiers CDS 129..1108 /dnas_title="AmpR" /label=AmpR /vntifkey="4" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_feature 2977..3054 /note="TCM Sig Seq" misc_recomb complement(3114..3142) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_feature 3055..3078 /note="8xHis Tag" misc_feature 3085..3108 /note="StrepTag" misc_feature 5208..5749 /note="WPRE" misc_recomb 5170..5192 /label=attB2 /note="attB2 site" /note="recognized as attB2" misc_feature complement(5756..5970) /note="BGH pA" misc_feature complement(6058..6187) /note="3'-ITR" misc_feature 3143..3165 /note="TEV site" source 3166..5163 /dnas_title="LARGE-pGEn1-DEST" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:151034 IMAGE:40125976" /tissue_type="Lung, PCR rescued clones" /clone_lib="NIH_MGC_299" /note="Vector: pCR4-TOPO with reversed insert; Clone identification sequence tag: ACCCAGTG" gene 3166..5163 /dnas_title="LARGE" /gene="LARGE" /gene_synonym="KIAA0609" /gene_synonym="MDC1D" /db_xref="GeneID:9215" /db_xref="HGNC:6511" /db_xref="MIM:603590" misc_feature 3166..5163 /dnas_title="LARGE" /gene="LARGE" /gene_synonym="KIAA0609" /gene_synonym="MDC1D" /note="; This cDNA sequence aligns to the human reference genome (build 36) without differences" CDS 3166..5163 /dnas_title="like-glycosyltransferase" /gene="LARGE" /gene_synonym="KIAA0609" /gene_synonym="MDC1D" /codon_start=1 /product="like-glycosyltransferase" /protein_id="AAI17426.1" /db_xref="GI:109659262" /db_xref="GeneID:9215" /db_xref="HGNC:6511" /db_xref="MIM:603590" /translation="MLGICRGRRKFLAASLSLLCIPAITWIYLFSGSFEDGKPVSLSP LESQAHSPRYTASSQRERESLEVRMREVEEENRALRRQLSLAQGRAPSHRRGNHSKTY SMEEGTGDSENLRAGIVAGNSSECGQQPVVEKCETIHVAIVCAGYNASRDVVTLVKSV LFHRRNPLHFHLIADSIAEQILATLFQTWMVPAVRVDFYNADELKSEVSWIPNKHYSG IYGLMKLVLTKTLPANLERVIVLDTDITFATDIAELWAVFHKFKGQQVLGLVENQSDW YLGNLWKNHRPWPALGRGYNTGVILLLLDKLRKMKWEQMWRLTAERELMGMLSTSLAD QDIFNAVIKQNPFLVYQLPCFWNVQLSDHTRSEQCYRDVSDLKVIHWNSPKKLRVKNK HVEFFRNLYLTFLEYDGNLLRRELFGCPSEADVNSENLQKQLSELDEDDLCYEFRRER FTVHRTHLYFLHYEYEPAADSTDVTLVAQLSMDRLQMLEAICKHWEGPISLALYLSDA EAQQFLRYAQGSEVLMSRHNVGYHIVYKEGQFYPVNLLRNVAMKHISTPYMFLSDIDF LPMYGLYEYLRKSVIQLDLANTKKAMIVPAFETLRYRLSFPKSKAELLSMLDMGTLFT FRYHVWTKGHAPTNFAKWRTATTPYRVEWEADFEPYVVVRRDCPEYDRRFVGFGWNKV AHIMELDVQEYEFIVLPNAYMIHMPHAPSFDITKFRSNKQYRICLKTLKEEFQQDMSR RYGFAALKYLTAENNS" misc_feature 3166..5163 /note="LARGE coding region" vector join(5178..6580,1..3132) /source="pGEn1-DEST" /type="Destination Vector" clone 3133..5177 /source="LARGE-Nterm-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCGCCATGA GACTGCTGAC AGCCCTGTTC 3001 GCCTACTTCA TCGTGGCCCT GATCCTGGCC TTCAGCGTGT CCGCCAAGAG CATGCACCAC 3061 CACCATCACC ATCATCACAG CGCCTGGTCC CACCCCCAGT TCGAGAAAGA ATTATCAACA 3121 AGTTTGTACA AAAAAGCAGG CTctgaaaac ttgtactttc aaggcccatc ccatcgccga 3181 ggcaaccact ccaagaccta ctccatggag gagggcactg gagacagcga gaaccttcgg 3241 gctggcatcg tggcaggcaa cagctccgag tgtgggcagc agccggtcgt ggagaaatgc 3301 gagacaatcc acgttgctat tgtctgcgcc ggatacaatg ccagccggga tgtcgtcacc 3361 ctggtcaaat ccgtcctgtt ccatagacgg aaccctctgc acttccacct tattgctgac 3421 tccattgcgg agcagatcct ggccacgctc ttccagacct ggatggtgcc cgctgtgcgt 3481 gtggacttct acaatgcaga cgagctcaag tctgaagttt cctggatccc caataaacat 3541 tactctggga tttatggtct gatgaagctt gtcctgacca agactcttcc tgccaacctg 3601 gagagagtca tcgtccttga cacggatatc acctttgcca ctgacattgc agagctgtgg 3661 gctgtgttcc acaagttcaa aggtcagcaa gtcctgggct tggtggagaa ccagagtgac 3721 tggtaccttg gaaacctgtg gaaaaatcac cgcccatggc cagcccttgg aagaggctac 3781 aacacagggg tgatcctgtt acttctggat aagctgcgga agatgaaatg ggagcagatg 3841 tggaggctga ccgcagagag ggagctcatg ggcatgctct ctacatcctt agctgaccag 3901 gatattttca atgccgtcat caaacaaaac cccttccttg tgtaccagct cccctgcttc 3961 tggaatgtgc agctgtcaga ccacacccgc tccgagcagt gctacagaga cgtgtctgat 4021 ctaaaggtca ttcactggaa ctcccccaag aagctccggg tgaagaacaa gcatgtggag 4081 ttttttcgca acctctacct gaccttcctg gagtatgacg gcaatcttct gaggcgggaa 4141 ctgtttggct gccccagtga ggctgatgtc aacagtgaaa acctccagaa gcagctgtct 4201 gagctggacg aggacgacct gtgctatgag ttccggcgag agcgcttcac tgtccaccgc 4261 acccacctgt acttcctgca ctacgagtat gagcctgcag cagacagcac ggacgtcacc 4321 ctggtcgctc agctgtccat ggacaggctc cagatgctgg aggccatctg caagcactgg 4381 gaggggccca tcagcctggc cctctacctg tcagacgccg aggcccagca gttcctccgc 4441 tacgcacagg gctctgaggt gcttatgagc cgccacaacg tgggctacca catcgtgtac 4501 aaggagggcc agttctaccc cgtgaacctg ctgcgcaacg tggccatgaa gcacatcagc 4561 actccctaca tgttcctgtc tgacattgac ttcctgccca tgtatgggct ctatgagtac 4621 ctcaggaagt ctgtcatcca gctcgatctt gccaacacca agaaagcaat gattgtcccc 4681 gcgttcgaga cactgcgcta ccggctgtcc ttccccaagt caaaagcgga gttgctgtca 4741 atgctggaca tggggaccct cttcacattc aggtaccacg tctggacgaa aggccacgca 4801 cccacaaact tcgccaagtg gcggaccgcc accacgcctt accgggttga gtgggaggcc 4861 gattttgagc cgtatgttgt tgtgagacgt gactgcccgg agtacgaccg gaggtttgta 4921 ggctttggct ggaacaaagt ggctcatatc atggagctgg atgtgcagga gtatgagttc 4981 attgtgctgc ccaacgccta catgatccac atgcctcatg cccccagctt cgacattacc 5041 aagttccgtt ccaacaagca ataccgcatc tgtctcaaaa ccctcaagga agagtttcag 5101 caggacatgt cccgccgcta cggctttgct gccctgaaat atctcacagc cgagaacaac 5161 agcTAGGACC CAGCTTTCTT GTACAAAGTG GTTGATAGCT TGGATCCAAT CAACCTCTGG 5221 ATTACAAAAT TTGTGAAAGA TTGACTGGTA TTCTTAACTA TGTTGCTCCT TTTACGCTAT 5281 GTGGATACGC TGCTTTAATG CCTTTGTATC ATGCTATTGC TTCCCGTATG GCTTTCATTT 5341 TCTCCTCCTT GTATAAATCC TGGTTGCTGT CTCTTTATGA GGAGTTGTGG CCCGTTGTCA 5401 GGCAACGTGG CGTGGTGTGC ACTGTGTTTG CTGACGCAAC CCCCACTGGT TGGGGCATTG 5461 CCACCACCTG TCAGCTCCTT TCCGGGACTT TCGCTTTCCC CCTCCCTATT GCCACGGCGG 5521 AACTCATCGC CGCCTGCCTT GCCCGCTGCT GGACAGGGGC TCGGCTGTTG GGCACTGACA 5581 ATTCCGTGGT GTTGTCGGGG AAGCTGACGT CCTTTCCATG GCTGCTCGCC TGTGTTGCCA 5641 CCTGGATTCT GCGCGGGACG TCCTTCTGCT ACGTCCCTTC GGCCCTCAAT CCAGCGGACC 5701 TTCCTTCCCG CGGCCTGCTG CCGGCTCTGC GGCCTCTTCC GCGTCTTCGA GATCTGCCTC 5761 GACTGTGCCT TCTAGTTGCC AGCCATCTGT TGTTTGCCCC TCCCCCGTGC CTTCCTTGAC 5821 CCTGGAAGGT GCCACTCCCA CTGTCCTTTC CTAATAAAAT GAGGAAATTG CATCGCATTG 5881 TCTGAGTAGG TGTCATTCTA TTCTGGGGGG TGGGGTGGGG CAGGACAGCA AGGGGGAGGA 5941 TTGGGAAGAC AATAGCAGGC ATGCTGGGGA CTCGAGTTAA GGGCGAATTG CCGATTAGGA 6001 TCTTCCTAGA GCATGGCTAC GTAGATAAGT AGCATGGCGG GTTAATCATT AACTACAAGG 6061 AACCCCTAGT GATGGAGTTG GCCACTCCCT CTCTGCGCGC TCGCTCGCTC ACTGAGGCCG 6121 GGCGACCAAA GGTCGCCCGA CGCCCGGGCT TTGCCCGGGC GGCCTCAGTG AGCGAGCGAG 6181 CGCGCAGCCT TAATTAAGAG CTCATGGCGC GCCTAGGCCT TGACGGCCTT CCGCCAATTC 6241 GCCCTATAGT GAGTCGTATT ACGTCGCGCT CACTGGCCGT CGTTTTACAA CGTCGTGACT 6301 GGGAAAACCC TGGCGTTACC CAACTTAATC GCCTTGCAGC ACATCCCCCT TTCGCCAGCT 6361 GGCGTAATAG CGAAGAGGCC CGCACCGAAA CGCCCTTCCC AACAGTTGCG CAGCCTGAAT 6421 GGCGAATGGG AGCGCCCTGT AGCGGCCACT CAACCCTATC TCGGTCTATT CTTTTGATTT 6481 ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT 6541 TAACGCGAAT TTTAACAAAA TATTAACGCT TACAATTTAG //