LOCUS       pDONR™/Zeo
DISCLAIMER  Certain terms are trademarks or registered trademarks of Invitrogen 
            Corporation. See "Intellectual Property" in the Help file for more 
            information.
FEATURES             Location/Qualifiers
     misc_feature    complement(268..295)
                     /note="rrnB T2 transcription termination sequence (c)"
     misc_feature    complement(427..470)
                     /note="rrnB T1 transcription termination sequence (c)"
     primer_bind     537..552
                     /note="M13 Forward (-20) priming site"
     misc_recomb     570..668
                     /label=attL1
                     /note="attL1"
     misc_recomb     complement(1703..1799)
                     /label=attL2
                     /note="attL2 (c)"
     misc_signal     complement(1814..1833)
                     /note="T7 Promoter/priming site (c)"
     primer_bind     1841..1857
                     /note="M13 Reverse priming site"
     gene            1970..2779
                     /note="Kanamycin resistance gene"
     rep_origin      2900..3573
                     /note="pUC origin"
     vector          join(1711..3576,1..651)
                     /source="pDONR%99221"
                     /type="Donor Vector"
     misc_feature    669..691
                     /note="TEV site"
     source          692..1696
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /clone="MGC:176582 IMAGE:9056717"
                     /tissue_type="Pooled, cerebellum, kidney, placenta,
                     testis, lung, colon, liver, heart, thyroid, bladder,
                     uterus, PCR rescued clones"
                     /clone_lib="NIH_MGC_361"
                     /lab_host="DH10B"
                     /note="Vector: pCR4-TOPO; Clone identification sequence
                     tag: TCCTTGTG"
     gene            692..1696
                     /gene="FUT5"
                     /gene_synonym=FUC-TV
                     /db_xref="GeneID:2527"
                     /db_xref="HGNC:4016"
                     /db_xref="MIM:136835"
     CDS             692..1696
                     /dnas_title="FUT5 protein"
                     /gene="FUT5"
                     /gene_synonym=FUC-TV
                     /codon_start=1
                     /product="FUT5 protein"
                     /protein_id="AAI40906.1"
                     /db_xref="GI:223461371"
                     /db_xref="GeneID:2527"
                     /db_xref="HGNC:4016"
                     /db_xref="MIM:136835"
                     /translation="MDPLGPAKPQWLWRRCLAGLLFQLLVAVCFFSYLRVSRDDATGS
                     PRPGLMAVEPVTGAPNGSRCQDSMATPAHPTLLILLWTWPFNTPVALPRCSEMVPGAA
                     DCNITADSSVYPQADAVIVHHWDIMYNPSANLPPPTRPQGQRWIWFSMESPSNCRHLE
                     ALDGYFNLTMSYRSDSDIFTPYGWLEPWSGQPAHPPLNLSAKTELVAWAVSNWKPDSA
                     RVRYYQSLQAHLKVDVYGRSHKPLPKGTMMETLSRYKFYLAFENSLHPDYITEKLWRN
                     ALEAWAVPVVLGPSRSNYERFLPPDAFIHVDDFQSPKDLARYLQELDKDHARYLSYFR
                     WRETLRPRSFSWALAFCKACWKLQQESRYQTVRSIAAWFT"
     misc_feature    692..1696
                     /note="FUT5"
ORIGIN      
          1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
         61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
        121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
        181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC
        241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA
        301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC
        361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA
        421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG
        481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA
        541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC
        601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA
        661 AGCAGGCTct gaaaacttgt actttcaagg cgatgccact ggatccccta ggccagggct
        721 tatggcagtg gaacctgtca ccggggctcc caatgggtcc cgctgccagg acagcatggc
        781 gacccctgcc caccccaccc tactgatcct gctgtggacg tggcctttta acacacccgt
        841 ggctctgccc cgctgctcag agatggtgcc cggcgcggcc gactgcaaca tcactgccga
        901 ctccagtgtg tacccacagg cagacgcggt catcgtgcac cactgggata tcatgtacaa
        961 ccccagtgcc aacctcccgc cccccaccag gccgcagggg cagcgctgga tctggttcag
       1021 catggagtcc cccagcaact gccggcacct ggaagccctg gacggatact tcaatctcac
       1081 catgtcctac cgcagcgact ccgacatctt cacgccctat ggctggctgg agccgtggtc
       1141 cggccagcct gcccacccac cgctcaacct ctcggccaag accgagctgg tggcctgggc
       1201 ggtgtccaac tggaagccgg actcggccag ggtgcgctac taccagagcc tgcaggctca
       1261 tctcaaggtg gacgtgtacg gacgctccca caagcccctg cccaagggga ccatgatgga
       1321 gacgctgtcc cggtacaagt tctatctggc cttcgagaac tccttgcacc ccgactacat
       1381 caccgagaag ctgtggagga acgccctgga ggcctgggcc gtgcccgtgg tgctgggccc
       1441 cagcagaagc aactacgaga ggttcctgcc acccgacgcc ttcatccacg tggatgactt
       1501 ccagagcccc aaggacctgg cccggtacct gcaggagctg gacaaggacc acgcccgcta
       1561 cctgagctac tttcgctggc gggagacgct gcggcctcgc tccttcagct gggcactggc
       1621 tttctgcaag gcctgctgga agctgcagca ggaatccagg taccagacgg tgcgcagcat
       1681 agcggcttgg ttcaccTAGG ACCCAGCTTT CTTGTACAAA GTTGGCATTA TAAGAAAGCA
       1741 TTGCTTATCA ATTTGTTGCA ACGAACAGGT CACTATCAGT CAAAATAAAA TCATTATTTG
       1801 CCATCCAGCT GATATCCCCT ATAGTGAGTC GTATTACATG GTCATAGCTG TTTCCTGGCA
       1861 GCTCTGGCCC GTGTCTCAAA ATCTCTGATG TTACATTGCA CAAGATAAAA TAATATCATC
       1921 ATGAACAATA AAACTGTCTG CTTACATAAA CAGTAATACA AGGGGTGTTA TGAGCCATAT
       1981 TCAACGGGAA ACGTCGAGGC CGCGATTAAA TTCCAACATG GATGCTGATT TATATGGGTA
       2041 TAAATGGGCT CGCGATAATG TCGGGCAATC AGGTGCGACA ATCTATCGCT TGTATGGGAA
       2101 GCCCGATGCG CCAGAGTTGT TTCTGAAACA TGGCAAAGGT AGCGTTGCCA ATGATGTTAC
       2161 AGATGAGATG GTCAGACTAA ACTGGCTGAC GGAATTTATG CCTCTTCCGA CCATCAAGCA
       2221 TTTTATCCGT ACTCCTGATG ATGCATGGTT ACTCACCACT GCGATCCCCG GAAAAACAGC
       2281 ATTCCAGGTA TTAGAAGAAT ATCCTGATTC AGGTGAAAAT ATTGTTGATG CGCTGGCAGT
       2341 GTTCCTGCGC CGGTTGCATT CGATTCCTGT TTGTAATTGT CCTTTTAACA GCGATCGCGT
       2401 ATTTCGTCTC GCTCAGGCGC AATCACGAAT GAATAACGGT TTGGTTGATG CGAGTGATTT
       2461 TGATGACGAG CGTAATGGCT GGCCTGTTGA ACAAGTCTGG AAAGAAATGC ATAAACTTTT
       2521 GCCATTCTCA CCGGATTCAG TCGTCACTCA TGGTGATTTC TCACTTGATA ACCTTATTTT
       2581 TGACGAGGGG AAATTAATAG GTTGTATTGA TGTTGGACGA GTCGGAATCG CAGACCGATA
       2641 CCAGGATCTT GCCATCCTAT GGAACTGCCT CGGTGAGTTT TCTCCTTCAT TACAGAAACG
       2701 GCTTTTTCAA AAATATGGTA TTGATAATCC TGATATGAAT AAATTGCAGT TTCATTTGAT
       2761 GCTCGATGAG TTTTTCTAAT CAGAATTGGT TAATTGGTTG TAACACTGGC AGAGCATTAC
       2821 GCTGACTTGA CGGGACGGCG CAAGCTCATG ACCAAAATCC CTTAACGTGA GTTACGCGTC
       2881 GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT
       2941 TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT
       3001 GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT
       3061 ACCAAATACT GTTCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC
       3121 ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA
       3181 GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG
       3241 CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG
       3301 ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG
       3361 GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA
       3421 CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT
       3481 GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG
       3541 GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTT
//