LOCUS FUT6-pGEn1-DEST 5542 bp DNA circular 22-JAN-2013 FEATURES Location/Qualifiers CDS 129..1108 /dnas_title="AmpR" /label=AmpR /vntifkey="4" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_feature 2977..3054 /note="TCM Sig Seq" misc_recomb complement(3114..3142) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_feature 3055..3078 /note="8xHis Tag" misc_feature 3085..3108 /note="StrepTag" misc_feature 4170..4711 /note="WPRE" misc_recomb 4132..4154 /label=attB2 /note="attB2 site" /note="recognized as attB2" misc_feature complement(4718..4932) /note="BGH pA" misc_feature complement(5020..5149) /note="3'-ITR" misc_feature 3143..3165 /note="TEV site" source 3166..4125 /dnas_title="FUT6-pGEn1-DEST" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:61990 IMAGE:4606746" /tissue_type="Kidney" /clone_lib="NIH_MGC_75" /lab_host="DH10B" /note="Vector: pDNR-LIB" gene 3166..4125 /dnas_title="FUT6" /gene="FUT6" /gene_synonym="FT1A" /db_xref="GeneID:2528" /db_xref="HGNC:4017" /db_xref="MIM:136836" CDS 3166..4125 /dnas_title="fucosyltransferase 6 (alpha (1,3) fucosyltransferase)" /gene="FUT6" /gene_synonym="FT1A" /codon_start=1 /product="fucosyltransferase 6 (alpha (1,3) fucosyltransferase)" /protein_id="AAH61700.1" /db_xref="GI:38512129" /db_xref="GeneID:2528" /db_xref="HGNC:4017" /db_xref="MIM:136836" /translation="MDPLGPAKPQWSWRCCLTTLLFHLLMAVCFFSYLRVSQDDPTVY PNGSRFPDSTGTPAHSIPLILLWTWPFNKPIALPRCSEMVPGTADCNITADRKVYPQA DAVIVHHREVMYNPSAQLPRSSRRQGQRWIWFSMESPSHCWQLKAMDGYFNLTMSYRS DSDIFTPYGWLEPWSGQPAHPPLNLSAKTELVAWAVSNWGPNSARVRYYQSLQAHLKV DVYGRSHKPLPQGTMMETLSRYKFYLAFKNSLHPDYITEKLWRNALEAWAVPVVLGPS RSNYERFLPPDAFIHVDDFQSPKDLARYLQELDKDHARYLSYFRWRETLRPRSFSWAL AFCKACWKLQEESRYQTRGIAAWFT" misc_difference 3418..3418 /dnas_title="FUT6" /gene="FUT6" /gene_synonym="FT1A" /note="'T' in cDNA is 'C' in the human genome; amino acid difference: 'S' in cDNA, 'P' in the human genome. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." misc_feature 3166..4125 /note="FUT6" misc_difference 3787..3787 /dnas_title="FUT6" /gene="FUT6" /gene_synonym="FT1A" /note="'A' in cDNA is 'G' in the human genome; amino acid difference: 'K' in cDNA, 'E' in the human genome. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." misc_difference 3903..3903 /dnas_title="FUT6" /gene="FUT6" /gene_synonym="FT1A" /note="'G' in cDNA is 'A' in the human genome; no amino acid change." vector join(4140..5542,1..3132) /source="pGEn1-DEST" /type="Destination Vector" clone 3133..4139 /source="FUT6-Nterm-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCGCCATGA GACTGCTGAC AGCCCTGTTC 3001 GCCTACTTCA TCGTGGCCCT GATCCTGGCC TTCAGCGTGT CCGCCAAGAG CATGCACCAC 3061 CACCATCACC ATCATCACAG CGCCTGGTCC CACCCCCAGT TCGAGAAAGA ATTATCAACA 3121 AGTTTGTACA AAAAAGCAGG CTctgaaaac ttgtactttc aaggcgatcc cactgtgtac 3181 cctaatgggt cccgcttccc agacagcaca gggacccccg cccactccat ccccctgatc 3241 ctgctgtgga cgtggccttt taacaaaccc atagctctgc cccgctgctc agagatggtg 3301 cctggcacgg ctgactgcaa catcactgcc gaccgcaagg tgtatccaca ggcagacgcg 3361 gtcatcgtgc accaccgaga ggtcatgtac aaccccagtg cccagctccc acgctcctcg 3421 aggcggcagg ggcagcgatg gatctggttc agcatggagt ccccaagcca ctgctggcag 3481 ctgaaagcca tggacggata cttcaatctc accatgtcct accgcagcga ctccgacatc 3541 ttcacgccct acggctggct ggagccgtgg tccggccagc ctgcccaccc accgctcaac 3601 ctctcggcca agaccgagct ggtggcctgg gcagtgtcca actgggggcc aaactccgcc 3661 agggtgcgct actaccagag cctgcaggcc catctcaagg tggacgtgta cggacgctcc 3721 cacaagcccc tgccccaggg aaccatgatg gagacgctgt cccggtacaa gttctatctg 3781 gccttcaaga actccttgca ccccgactac atcaccgaga agctgtggag gaacgccctg 3841 gaggcctggg ccgtgcccgt ggtgctgggc cccagcagaa gcaactacga gaggttcctg 3901 ccgcccgacg ccttcatcca cgtggacgac ttccagagcc ccaaggacct ggcccggtac 3961 ctgcaggagc tggacaagga ccacgcccgc tacctgagct actttcgctg gcgggagacg 4021 ctgcggcctc gctccttcag ctgggcactc gctttctgca aggcctgctg gaaactgcag 4081 gaggaatcca ggtaccagac acgcggcata gcggcttggt tcaccTAGGA CCCAGCTTTC 4141 TTGTACAAAG TGGTTGATAG CTTGGATCCA ATCAACCTCT GGATTACAAA ATTTGTGAAA 4201 GATTGACTGG TATTCTTAAC TATGTTGCTC CTTTTACGCT ATGTGGATAC GCTGCTTTAA 4261 TGCCTTTGTA TCATGCTATT GCTTCCCGTA TGGCTTTCAT TTTCTCCTCC TTGTATAAAT 4321 CCTGGTTGCT GTCTCTTTAT GAGGAGTTGT GGCCCGTTGT CAGGCAACGT GGCGTGGTGT 4381 GCACTGTGTT TGCTGACGCA ACCCCCACTG GTTGGGGCAT TGCCACCACC TGTCAGCTCC 4441 TTTCCGGGAC TTTCGCTTTC CCCCTCCCTA TTGCCACGGC GGAACTCATC GCCGCCTGCC 4501 TTGCCCGCTG CTGGACAGGG GCTCGGCTGT TGGGCACTGA CAATTCCGTG GTGTTGTCGG 4561 GGAAGCTGAC GTCCTTTCCA TGGCTGCTCG CCTGTGTTGC CACCTGGATT CTGCGCGGGA 4621 CGTCCTTCTG CTACGTCCCT TCGGCCCTCA ATCCAGCGGA CCTTCCTTCC CGCGGCCTGC 4681 TGCCGGCTCT GCGGCCTCTT CCGCGTCTTC GAGATCTGCC TCGACTGTGC CTTCTAGTTG 4741 CCAGCCATCT GTTGTTTGCC CCTCCCCCGT GCCTTCCTTG ACCCTGGAAG GTGCCACTCC 4801 CACTGTCCTT TCCTAATAAA ATGAGGAAAT TGCATCGCAT TGTCTGAGTA GGTGTCATTC 4861 TATTCTGGGG GGTGGGGTGG GGCAGGACAG CAAGGGGGAG GATTGGGAAG ACAATAGCAG 4921 GCATGCTGGG GACTCGAGTT AAGGGCGAAT TGCCGATTAG GATCTTCCTA GAGCATGGCT 4981 ACGTAGATAA GTAGCATGGC GGGTTAATCA TTAACTACAA GGAACCCCTA GTGATGGAGT 5041 TGGCCACTCC CTCTCTGCGC GCTCGCTCGC TCACTGAGGC CGGGCGACCA AAGGTCGCCC 5101 GACGCCCGGG CTTTGCCCGG GCGGCCTCAG TGAGCGAGCG AGCGCGCAGC CTTAATTAAG 5161 AGCTCATGGC GCGCCTAGGC CTTGACGGCC TTCCGCCAAT TCGCCCTATA GTGAGTCGTA 5221 TTACGTCGCG CTCACTGGCC GTCGTTTTAC AACGTCGTGA CTGGGAAAAC CCTGGCGTTA 5281 CCCAACTTAA TCGCCTTGCA GCACATCCCC CTTTCGCCAG CTGGCGTAAT AGCGAAGAGG 5341 CCCGCACCGA AACGCCCTTC CCAACAGTTG CGCAGCCTGA ATGGCGAATG GGAGCGCCCT 5401 GTAGCGGCCA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT 5461 TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTTAACAA 5521 AATATTAACG CTTACAATTT AG //