LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(1661..1757) /label=attL2 /note="attL2 (c)" misc_signal complement(1772..1791) /note="T7 Promoter/priming site (c)" primer_bind 1799..1815 /note="M13 Reverse priming site" gene 1928..2737 /note="Kanamycin resistance gene" rep_origin 2858..3531 /note="pUC origin" vector join(1669..3534,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 669..691 /note="TEV site" source 692..1654 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:33876 IMAGE:5286309" /tissue_type="Brain, hypothalamus" /clone_lib="NIH_MGC_96" /lab_host="DH10B" /note="Vector: pBluescriptR" gene 692..1654 /gene="FUT9" /gene_synonym=Fuc-TIX /db_xref="GeneID:10690" /db_xref="HGNC:4020" /db_xref="MIM:606865" CDS 692..1654 /dnas_title="fucosyltransferase 9 (alpha (1,3) fucosyltransferase)" /gene="FUT9" /gene_synonym=Fuc-TIX /codon_start=1 /product="fucosyltransferase 9 (alpha (1,3) fucosyltransferase)" /protein_id="AAH36101.1" /db_xref="GI:23273585" /db_xref="GeneID:10690" /db_xref="HGNC:4020" /db_xref="MIM:606865" /translation="MTSTSKGILRPFLIVCIILGCFMACLLIYIKPTNSWIFSPMESA SSVLKMKNFFSTKTDYFNETTILVWVWPFGQTFDLTSCQAMFNIQGCHLTTDRSLYNK SHAVLIHHRDISWDLTNLPQQARPPFQKWIWMNLESPTHTPQKSGIEHLFNLTLTYRR DSDIQVPYGFLTVSTNPFVFEVPSKEKLVCWVVSNWNPEHARVKYYNELSKSIEIHTY GQAFGEYVNDKNLIPTISACKFYLSFENSIHKDYITEKLYNAFLAGSVPVVLGPSREN YENYIPADSFIHVEDYNSPSELAKYLKEVDKNNKLYLSYFNWRKDFTVNLPRFWESHA CLACDHVKRHQEYKSVGNLEKWFWN" misc_feature 692..1654 /note="FUT9" misc_difference 1286..1286 /gene="FUT9" /gene_synonym=Fuc-TIX /note="'G' in cDNA is 'A' in the human genome; amino acid difference: 'A' in cDNA, 'T' in the human genome. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg cagtccaatg gaatcagcca gctctgtgct 721 gaaaatgaaa aacttctttt ccaccaaaac tgattatttt aatgaaacta ctattctggt 781 gtgggtgtgg ccatttgggc agacctttga ccttacatcc tgccaagcaa tgttcaacat 841 ccaaggatgc catctcacaa cggaccgttc actgtacaac aaatcccatg cagttctgat 901 ccatcaccga gacatcagtt gggatctgac aaatttacct cagcaagcta ggccaccctt 961 ccagaaatgg atttggatga atttggaatc accaactcac actccccaaa agagtggcat 1021 tgagcacttg tttaacctga ctctgactta ccgccgtgat tcagatatcc aagtgcctta 1081 tggcttcttg acggtaagca caaatccctt cgtgtttgaa gtgccaagca aagagaaatt 1141 ggtgtgctgg gttgtgagta actggaaccc tgagcatgcc agagtcaagt attacaatga 1201 gctaagcaaa agcattgaaa tccataccta cgggcaagca tttggagaat atgtcaatga 1261 taaaaatttg attcctacca tatctgcttg taaattttat ctttcctttg aaaattcaat 1321 ccacaaggat tacatcacgg aaaagctata caatgctttt ctggctggct ctgtacctgt 1381 tgttctggga ccatctaggg aaaactatga gaattatatt ccagcagatt cattcattca 1441 tgtggaagat tataactctc ccagtgagct agcaaagtat ctgaaggaag tcgacaaaaa 1501 caataagtta taccttagtt actttaactg gaggaaggat ttcactgtaa atcttccacg 1561 attttgggaa tcacatgcat gtttggcttg cgatcatgtg aaaaggcatc aagaatataa 1621 gtctgttggt aatttagaga aatggttttg gaatTAGGAC CCAGCTTTCT TGTACAAAGT 1681 TGGCATTATA AGAAAGCATT GCTTATCAAT TTGTTGCAAC GAACAGGTCA CTATCAGTCA 1741 AAATAAAATC ATTATTTGCC ATCCAGCTGA TATCCCCTAT AGTGAGTCGT ATTACATGGT 1801 CATAGCTGTT TCCTGGCAGC TCTGGCCCGT GTCTCAAAAT CTCTGATGTT ACATTGCACA 1861 AGATAAAATA ATATCATCAT GAACAATAAA ACTGTCTGCT TACATAAACA GTAATACAAG 1921 GGGTGTTATG AGCCATATTC AACGGGAAAC GTCGAGGCCG CGATTAAATT CCAACATGGA 1981 TGCTGATTTA TATGGGTATA AATGGGCTCG CGATAATGTC GGGCAATCAG GTGCGACAAT 2041 CTATCGCTTG TATGGGAAGC CCGATGCGCC AGAGTTGTTT CTGAAACATG GCAAAGGTAG 2101 CGTTGCCAAT GATGTTACAG ATGAGATGGT CAGACTAAAC TGGCTGACGG AATTTATGCC 2161 TCTTCCGACC ATCAAGCATT TTATCCGTAC TCCTGATGAT GCATGGTTAC TCACCACTGC 2221 GATCCCCGGA AAAACAGCAT TCCAGGTATT AGAAGAATAT CCTGATTCAG GTGAAAATAT 2281 TGTTGATGCG CTGGCAGTGT TCCTGCGCCG GTTGCATTCG ATTCCTGTTT GTAATTGTCC 2341 TTTTAACAGC GATCGCGTAT TTCGTCTCGC TCAGGCGCAA TCACGAATGA ATAACGGTTT 2401 GGTTGATGCG AGTGATTTTG ATGACGAGCG TAATGGCTGG CCTGTTGAAC AAGTCTGGAA 2461 AGAAATGCAT AAACTTTTGC CATTCTCACC GGATTCAGTC GTCACTCATG GTGATTTCTC 2521 ACTTGATAAC CTTATTTTTG ACGAGGGGAA ATTAATAGGT TGTATTGATG TTGGACGAGT 2581 CGGAATCGCA GACCGATACC AGGATCTTGC CATCCTATGG AACTGCCTCG GTGAGTTTTC 2641 TCCTTCATTA CAGAAACGGC TTTTTCAAAA ATATGGTATT GATAATCCTG ATATGAATAA 2701 ATTGCAGTTT CATTTGATGC TCGATGAGTT TTTCTAATCA GAATTGGTTA ATTGGTTGTA 2761 ACACTGGCAG AGCATTACGC TGACTTGACG GGACGGCGCA AGCTCATGAC CAAAATCCCT 2821 TAACGTGAGT TACGCGTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC 2881 TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT 2941 ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG 3001 CTTCAGCAGA GCGCAGATAC CAAATACTGT TCTTCTAGTG TAGCCGTAGT TAGGCCACCA 3061 CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC 3121 TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA 3181 TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC 3241 GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA 3301 AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG 3361 GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG 3421 ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG 3481 CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTT //