LOCUS FUT9-pGEn1-DEST 5545 bp DNA circular 22-JAN-2013 FEATURES Location/Qualifiers CDS 129..1108 /dnas_title="AmpR" /label=AmpR /vntifkey="4" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_feature 2977..3054 /note="TCM Sig Seq" misc_recomb complement(3114..3142) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_feature 3055..3078 /note="8xHis Tag" misc_feature 3085..3108 /note="StrepTag" misc_feature 4173..4714 /note="WPRE" misc_recomb 4135..4157 /label=attB2 /note="attB2 site" /note="recognized as attB2" misc_feature complement(4721..4935) /note="BGH pA" misc_feature complement(5023..5152) /note="3'-ITR" misc_feature 3143..3165 /note="TEV site" source 3166..4128 /dnas_title="FUT9-pGEn1-DEST" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:33876 IMAGE:5286309" /tissue_type="Brain, hypothalamus" /clone_lib="NIH_MGC_96" /lab_host="DH10B" /note="Vector: pBluescriptR" gene 3166..4128 /dnas_title="FUT9" /gene="FUT9" /gene_synonym="Fuc-TIX" /db_xref="GeneID:10690" /db_xref="HGNC:4020" /db_xref="MIM:606865" CDS 3166..4128 /dnas_title="fucosyltransferase 9 (alpha (1,3) fucosyltransferase)" /gene="FUT9" /gene_synonym="Fuc-TIX" /codon_start=1 /product="fucosyltransferase 9 (alpha (1,3) fucosyltransferase)" /protein_id="AAH36101.1" /db_xref="GI:23273585" /db_xref="GeneID:10690" /db_xref="HGNC:4020" /db_xref="MIM:606865" /translation="MTSTSKGILRPFLIVCIILGCFMACLLIYIKPTNSWIFSPMESA SSVLKMKNFFSTKTDYFNETTILVWVWPFGQTFDLTSCQAMFNIQGCHLTTDRSLYNK SHAVLIHHRDISWDLTNLPQQARPPFQKWIWMNLESPTHTPQKSGIEHLFNLTLTYRR DSDIQVPYGFLTVSTNPFVFEVPSKEKLVCWVVSNWNPEHARVKYYNELSKSIEIHTY GQAFGEYVNDKNLIPTISACKFYLSFENSIHKDYITEKLYNAFLAGSVPVVLGPSREN YENYIPADSFIHVEDYNSPSELAKYLKEVDKNNKLYLSYFNWRKDFTVNLPRFWESHA CLACDHVKRHQEYKSVGNLEKWFWN" misc_feature 3166..4128 /note="FUT9" misc_difference 3760..3760 /dnas_title="FUT9" /gene="FUT9" /gene_synonym="Fuc-TIX" /note="'G' in cDNA is 'A' in the human genome; amino acid difference: 'A' in cDNA, 'T' in the human genome. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." vector join(4143..5545,1..3132) /source="pGEn1-DEST" /type="Destination Vector" clone 3133..4142 /source="FUT9-Nterm-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCGCCATGA GACTGCTGAC AGCCCTGTTC 3001 GCCTACTTCA TCGTGGCCCT GATCCTGGCC TTCAGCGTGT CCGCCAAGAG CATGCACCAC 3061 CACCATCACC ATCATCACAG CGCCTGGTCC CACCCCCAGT TCGAGAAAGA ATTATCAACA 3121 AGTTTGTACA AAAAAGCAGG CTctgaaaac ttgtactttc aaggcagtcc aatggaatca 3181 gccagctctg tgctgaaaat gaaaaacttc ttttccacca aaactgatta ttttaatgaa 3241 actactattc tggtgtgggt gtggccattt gggcagacct ttgaccttac atcctgccaa 3301 gcaatgttca acatccaagg atgccatctc acaacggacc gttcactgta caacaaatcc 3361 catgcagttc tgatccatca ccgagacatc agttgggatc tgacaaattt acctcagcaa 3421 gctaggccac ccttccagaa atggatttgg atgaatttgg aatcaccaac tcacactccc 3481 caaaagagtg gcattgagca cttgtttaac ctgactctga cttaccgccg tgattcagat 3541 atccaagtgc cttatggctt cttgacggta agcacaaatc ccttcgtgtt tgaagtgcca 3601 agcaaagaga aattggtgtg ctgggttgtg agtaactgga accctgagca tgccagagtc 3661 aagtattaca atgagctaag caaaagcatt gaaatccata cctacgggca agcatttgga 3721 gaatatgtca atgataaaaa tttgattcct accatatctg cttgtaaatt ttatctttcc 3781 tttgaaaatt caatccacaa ggattacatc acggaaaagc tatacaatgc ttttctggct 3841 ggctctgtac ctgttgttct gggaccatct agggaaaact atgagaatta tattccagca 3901 gattcattca ttcatgtgga agattataac tctcccagtg agctagcaaa gtatctgaag 3961 gaagtcgaca aaaacaataa gttatacctt agttacttta actggaggaa ggatttcact 4021 gtaaatcttc cacgattttg ggaatcacat gcatgtttgg cttgcgatca tgtgaaaagg 4081 catcaagaat ataagtctgt tggtaattta gagaaatggt tttggaatTA GGACCCAGCT 4141 TTCTTGTACA AAGTGGTTGA TAGCTTGGAT CCAATCAACC TCTGGATTAC AAAATTTGTG 4201 AAAGATTGAC TGGTATTCTT AACTATGTTG CTCCTTTTAC GCTATGTGGA TACGCTGCTT 4261 TAATGCCTTT GTATCATGCT ATTGCTTCCC GTATGGCTTT CATTTTCTCC TCCTTGTATA 4321 AATCCTGGTT GCTGTCTCTT TATGAGGAGT TGTGGCCCGT TGTCAGGCAA CGTGGCGTGG 4381 TGTGCACTGT GTTTGCTGAC GCAACCCCCA CTGGTTGGGG CATTGCCACC ACCTGTCAGC 4441 TCCTTTCCGG GACTTTCGCT TTCCCCCTCC CTATTGCCAC GGCGGAACTC ATCGCCGCCT 4501 GCCTTGCCCG CTGCTGGACA GGGGCTCGGC TGTTGGGCAC TGACAATTCC GTGGTGTTGT 4561 CGGGGAAGCT GACGTCCTTT CCATGGCTGC TCGCCTGTGT TGCCACCTGG ATTCTGCGCG 4621 GGACGTCCTT CTGCTACGTC CCTTCGGCCC TCAATCCAGC GGACCTTCCT TCCCGCGGCC 4681 TGCTGCCGGC TCTGCGGCCT CTTCCGCGTC TTCGAGATCT GCCTCGACTG TGCCTTCTAG 4741 TTGCCAGCCA TCTGTTGTTT GCCCCTCCCC CGTGCCTTCC TTGACCCTGG AAGGTGCCAC 4801 TCCCACTGTC CTTTCCTAAT AAAATGAGGA AATTGCATCG CATTGTCTGA GTAGGTGTCA 4861 TTCTATTCTG GGGGGTGGGG TGGGGCAGGA CAGCAAGGGG GAGGATTGGG AAGACAATAG 4921 CAGGCATGCT GGGGACTCGA GTTAAGGGCG AATTGCCGAT TAGGATCTTC CTAGAGCATG 4981 GCTACGTAGA TAAGTAGCAT GGCGGGTTAA TCATTAACTA CAAGGAACCC CTAGTGATGG 5041 AGTTGGCCAC TCCCTCTCTG CGCGCTCGCT CGCTCACTGA GGCCGGGCGA CCAAAGGTCG 5101 CCCGACGCCC GGGCTTTGCC CGGGCGGCCT CAGTGAGCGA GCGAGCGCGC AGCCTTAATT 5161 AAGAGCTCAT GGCGCGCCTA GGCCTTGACG GCCTTCCGCC AATTCGCCCT ATAGTGAGTC 5221 GTATTACGTC GCGCTCACTG GCCGTCGTTT TACAACGTCG TGACTGGGAA AACCCTGGCG 5281 TTACCCAACT TAATCGCCTT GCAGCACATC CCCCTTTCGC CAGCTGGCGT AATAGCGAAG 5341 AGGCCCGCAC CGAAACGCCC TTCCCAACAG TTGCGCAGCC TGAATGGCGA ATGGGAGCGC 5401 CCTGTAGCGG CCACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC 5461 GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTTAA 5521 CAAAATATTA ACGCTTACAA TTTAG //