LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(1946..2042) /label=attL2 /note="attL2 (c)" misc_signal complement(2057..2076) /note="T7 Promoter/priming site (c)" primer_bind 2084..2100 /note="M13 Reverse priming site" gene 2213..3022 /note="Kanamycin resistance gene" rep_origin 3143..3816 /note="pUC origin" vector join(1954..3819,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 669..691 /note="TEV site" source 692..1939 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:2304 IMAGE:3544942" /tissue_type="Kidney, renal cell adenocarcinoma" /clone_lib="NIH_MGC_14" /lab_host="DH10B-R" /note="Vector: pOTB7" gene 692..1939 /gene="MGAT1" /gene_synonym=GLCNAC-TI /gene_synonym=GLCT1 /gene_synonym=GNT-1 /gene_synonym=GNT-I /db_xref="GeneID:4245" /db_xref="MIM:160995" CDS 692..1939 /dnas_title="MGAT1 protein" /gene="MGAT1" /gene_synonym=GLCNAC-TI /gene_synonym=GLCT1 /gene_synonym=GNT-1 /gene_synonym=GNT-I /codon_start=1 /product="MGAT1 protein" /protein_id="AAH03575.1" /db_xref="GI:13097753" /db_xref="GeneID:4245" /db_xref="MIM:160995" /translation="MLKKQSAGLVLWGAILFVAWNALLLLFFWTRPAPGRPPSVSALD GDPASLTREVIRLAQDAEVELERQRGLLQQIGDALSSQRGRVPTAAPPAQPRVPVTPA PAVIPILVIACDRSTVRRCLDKLLHYRPSAELFPIIVSQDCGHEETAQAIASYGSAVT HIRQPDLSSIAVPPDHRKFQGYYKIARHYRWALGQVFRQFRFPAAVVVEDDLEVAPDF FEYFRATYPLLKADPSLWCVSAWNDNGKEQMVDASRPELLYRTDFFPGLGWLLLAELW AELEPKWPKAFWDDWMRRPEQRQGRACIRPEISRTMTFGRKGVSHGQFFDQHLKFIKL NQQFVHFTQLDLSYLQREAYDRDFLARVYGAPQLQVEKVRTNDRKELGEVRVQYTGRD SFKAFAKALGVMDDLKSGVPRAGYRGIVTFQFRGRRVHLAPPPTWEGYDPSWN" misc_feature 692..1939 /note="MGAT1 coding region" misc_difference 1908..1908 /gene="MGAT1" /gene_synonym=GLCNAC-TI /gene_synonym=GLCT1 /gene_synonym=GNT-1 /gene_synonym=GNT-I /note="'C' in cDNA is 'T' in the human genome; amino acid difference: 'P' in cDNA, 'L' in the human genome. The chimpanzee genome agrees with the cDNA sequence, suggesting that this difference is unlikely to be due to an artifact." ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg cacgcgccca gcacctggca ggccaccctc 721 agtcagcgct ctcgatggcg accccgccag cctcacccgg gaagtgattc gcctggccca 781 agacgccgag gtggagctgg agcggcagcg tgggctgctg cagcagatcg gggatgccct 841 gtcgagccag cgggggaggg tgcccaccgc ggcccctccc gcccagccgc gtgtgcctgt 901 gacccccgcg ccggcggtga ttcccatcct ggtcatcgcc tgtgaccgca gcactgttcg 961 gcgctgcctg gacaagctgc tgcattatcg gccctcggct gagctcttcc ccatcatcgt 1021 tagccaggac tgcgggcacg aggagacggc ccaggccatc gcctcctacg gcagcgcggt 1081 cacgcacatc cggcagcccg acctgagcag cattgcggtg ccgccggacc accgcaagtt 1141 ccagggctac tacaagatcg cgcgccacta ccgctgggcg ctgggccagg tcttccggca 1201 gtttcgcttc cccgcggccg tggtggtgga ggatgacctg gaggtggccc cggacttctt 1261 cgagtacttt cgggccacct atccgctgct gaaggccgac ccctccctgt ggtgcgtctc 1321 ggcctggaat gacaacggca aggagcagat ggtggacgcc agcaggcctg agctgctcta 1381 ccgcaccgac tttttccctg gcctgggctg gctgctgttg gccgagctct gggctgagct 1441 ggagcccaag tggccaaagg ccttctggga cgactggatg cggcggccgg agcagcggca 1501 ggggcgggcc tgcatacgcc ctgagatctc aagaacgatg acctttggcc gcaagggtgt 1561 gagccacggg cagttctttg accagcacct caagtttatc aagctgaacc agcagtttgt 1621 gcacttcacc cagctggacc tgtcttacct gcagcgggag gcctatgacc gagatttcct 1681 cgcccgcgtc tacggtgctc cccagctgca ggtggagaaa gtgaggacca atgaccggaa 1741 ggagctgggg gaggtgcggg tgcagtatac gggcagggac agcttcaagg ctttcgccaa 1801 ggctctgggt gtcatggatg accttaagtc gggggttccg agagctggct accggggtat 1861 tgtcaccttc cagttccggg gccgccgtgt ccacctggcg cccccaccga cgtgggaggg 1921 ctatgatcct agctggaatT AGGACCCAGC TTTCTTGTAC AAAGTTGGCA TTATAAGAAA 1981 GCATTGCTTA TCAATTTGTT GCAACGAACA GGTCACTATC AGTCAAAATA AAATCATTAT 2041 TTGCCATCCA GCTGATATCC CCTATAGTGA GTCGTATTAC ATGGTCATAG CTGTTTCCTG 2101 GCAGCTCTGG CCCGTGTCTC AAAATCTCTG ATGTTACATT GCACAAGATA AAATAATATC 2161 ATCATGAACA ATAAAACTGT CTGCTTACAT AAACAGTAAT ACAAGGGGTG TTATGAGCCA 2221 TATTCAACGG GAAACGTCGA GGCCGCGATT AAATTCCAAC ATGGATGCTG ATTTATATGG 2281 GTATAAATGG GCTCGCGATA ATGTCGGGCA ATCAGGTGCG ACAATCTATC GCTTGTATGG 2341 GAAGCCCGAT GCGCCAGAGT TGTTTCTGAA ACATGGCAAA GGTAGCGTTG CCAATGATGT 2401 TACAGATGAG ATGGTCAGAC TAAACTGGCT GACGGAATTT ATGCCTCTTC CGACCATCAA 2461 GCATTTTATC CGTACTCCTG ATGATGCATG GTTACTCACC ACTGCGATCC CCGGAAAAAC 2521 AGCATTCCAG GTATTAGAAG AATATCCTGA TTCAGGTGAA AATATTGTTG ATGCGCTGGC 2581 AGTGTTCCTG CGCCGGTTGC ATTCGATTCC TGTTTGTAAT TGTCCTTTTA ACAGCGATCG 2641 CGTATTTCGT CTCGCTCAGG CGCAATCACG AATGAATAAC GGTTTGGTTG ATGCGAGTGA 2701 TTTTGATGAC GAGCGTAATG GCTGGCCTGT TGAACAAGTC TGGAAAGAAA TGCATAAACT 2761 TTTGCCATTC TCACCGGATT CAGTCGTCAC TCATGGTGAT TTCTCACTTG ATAACCTTAT 2821 TTTTGACGAG GGGAAATTAA TAGGTTGTAT TGATGTTGGA CGAGTCGGAA TCGCAGACCG 2881 ATACCAGGAT CTTGCCATCC TATGGAACTG CCTCGGTGAG TTTTCTCCTT CATTACAGAA 2941 ACGGCTTTTT CAAAAATATG GTATTGATAA TCCTGATATG AATAAATTGC AGTTTCATTT 3001 GATGCTCGAT GAGTTTTTCT AATCAGAATT GGTTAATTGG TTGTAACACT GGCAGAGCAT 3061 TACGCTGACT TGACGGGACG GCGCAAGCTC ATGACCAAAA TCCCTTAACG TGAGTTACGC 3121 GTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT 3181 TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG 3241 TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA 3301 GATACCAAAT ACTGTTCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT 3361 AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA 3421 TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC 3481 GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT 3541 GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA 3601 CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG 3661 AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT 3721 TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT 3781 ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTT //