LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2501..2597) /label=attL2 /note="attL2 (c)" misc_signal complement(2612..2631) /note="T7 Promoter/priming site (c)" primer_bind 2639..2655 /note="M13 Reverse priming site" gene 2768..3577 /note="Kanamycin resistance gene" rep_origin 3698..4371 /note="pUC origin" vector join(2509..4374,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 669..691 /note="TEV site" source 692..2494 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:1063 IMAGE:3138971" /tissue_type="Placenta, choriocarcinoma" /clone_lib="NIH_MGC_21" /lab_host="DH10B-R" /note="Vector: pOTB7" gene 692..2494 /gene="POMGNT1" /gene_synonym=FLJ20277 /gene_synonym=GnTI.2 /gene_synonym=MEB /gene_synonym=MGAT1.2 /db_xref="GeneID:55624" /db_xref="HGNC:19139" /db_xref="MIM:606822" CDS 692..2494 /dnas_title="protein O-linked mannose beta1,2-N-acetylglucosaminyltransferase" /gene="POMGNT1" /gene_synonym=FLJ20277 /gene_synonym=GnTI.2 /gene_synonym=MEB /gene_synonym=MGAT1.2 /codon_start=1 /product="protein O-linked mannose beta1,2-N-acetylglucosaminyltransferase" /protein_id="AAH01471.1" /db_xref="GI:12655225" /db_xref="GeneID:55624" /db_xref="HGNC:19139" /db_xref="MIM:606822" /translation="MDDWKPSPLIKPFGARKKRSWYLTWKYKLTNQRALRRFCQTGAV LFLLVTVIVNIKLILDTRRAISEANEDPEPEQDYDEALGRLEPPRRRGSGPRRVLDVE VYSSRSKVYVAVDGTTVLEDEAREQGRGIHVIVLNQATGHVMAKRVFDTYSPHEDEAM VLFLNMVAPGRVLICTVKDEGSFHLKDTAKALLRSLGSQAGPALGWRDTWAFVGRKGG PVFGEKHSKSPALSSWGDPVLLKTDVPLSSAVEAECHWADTELNRRRRRFCSKVEGYG SVCSCKDPTPIEFSPDPLPDNKVLNVPVAVIAGNRPNYLYRMLRSLLSAQGVSPQMIT VFIDGYYEEPMDVVALFGLRGIQHTPISIKNARVSQHYKASLTATFNLFPEAKFAVVL EEDLDIAVDFFSFLSQSIHLLEEDDSLYCISAWNDQGYEHTAEDPALLYRVETMPGLG WVLRRSLYKEELEPKWPTPEKLWDWDMWMRMPEQRRGRECIIPDVSRSYHFGIVGLNM NGYFHEAYFKKHKFNTVPGVQLRNVDSLKKEAYEVEVHRLLSEAEVLDHSKNPCEDSF LPDTEGHTYVAFIRMEKDDDFTTWTQLAKCLHIWDLDVRGNHRGLWRLFRKKNHFLVV GVPASPYSVKKPPSVTPIFLEPPPKEEGAPGAPEQT" misc_feature 692..2494 /note="POMGNT1 coding region" misc_difference 1263..1263 /gene="POMGNT1" /gene_synonym=FLJ20277 /gene_synonym=GnTI.2 /gene_synonym=MEB /gene_synonym=MGAT1.2 /note="'T' in cDNA is 'A' in the human genome; amino acid difference: 'V' in cDNA, 'E' in the human genome. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." misc_difference 2381..2381 /gene="POMGNT1" /gene_synonym=FLJ20277 /gene_synonym=GnTI.2 /gene_synonym=MEB /gene_synonym=MGAT1.2 /note="'G' in cDNA is 'A' in the human genome; amino acid difference: 'V' in cDNA, 'M' in the human genome. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg cgacactcgg cgagccatca gtgaagccaa 721 tgaagaccca gagccagagc aagactatga tgaggcccta ggccgcctgg agcccccacg 781 gcgcagaggc agtggtcccc ggcgggtcct ggacgtagag gtgtattcaa gtcgcagcaa 841 agtatatgtg gcagtggatg gcaccacggt gctggaggat gaggcccggg agcagggccg 901 gggcatccat gtcattgtcc tcaaccaggc cacgggccac gtgatggcaa aacgtgtgtt 961 tgacacgtac tcacctcatg aggatgaggc catggtgcta ttcctcaaca tggtagcgcc 1021 cggccgagtg ctcatctgca ctgtcaagga tgagggctcc ttccacctca aggacacagc 1081 caaggctctg ctgaggagcc tgggcagcca ggctggccct gccctgggct ggagggacac 1141 atgggccttc gtgggacgaa aaggaggtcc tgtcttcggg gagaaacatt ctaaatcacc 1201 tgccctctct tcctgggggg acccagtcct gctgaagaca gatgtgccat tgagctcagc 1261 agtagaggca gagtgccact gggcagacac agagctgaac cgtcgccgcc ggcgcttctg 1321 cagcaaagtt gagggctatg gaagtgtatg cagctgcaag gaccccacac ccatcgagtt 1381 cagccctgac ccactcccag acaacaaggt cctcaatgtg cctgtggctg tcattgcagg 1441 gaaccgaccc aattacctgt acaggatgct gcgctctctg ctttcagccc agggggtgtc 1501 tcctcagatg ataacagttt tcattgacgg ctactatgag gaacccatgg atgtggtggc 1561 actgtttggt ctgaggggca tccagcatac tcccatcagc atcaagaatg cccgcgtgtc 1621 tcagcactac aaggccagcc tcactgccac tttcaacctg tttccggagg ccaagtttgc 1681 tgtggttctg gaagaggacc tggacattgc tgtggatttt ttcagtttcc tgagccaatc 1741 catccaccta ctggaggagg atgacagcct gtactgcatc tctgcctgga atgaccaggg 1801 gtatgaacac acggctgagg acccagcact actgtaccgt gtggagacca tgcctgggct 1861 gggctgggtg ctcaggaggt ccttgtacaa ggaggagctt gagcccaagt ggcctacacc 1921 ggaaaagctc tgggattggg acatgtggat gcggatgcct gaacaacgcc ggggccgaga 1981 gtgcatcatc cctgacgttt cccgatccta ccactttggc atcgtcggcc tcaacatgaa 2041 tggctacttt cacgaggcct acttcaagaa gcacaagttc aacacggttc caggtgtcca 2101 gctcaggaat gtggacagtc tgaagaaaga agcttatgaa gtggaagttc acaggctgct 2161 cagtgaggct gaggttctgg accacagcaa gaacccttgt gaagactctt tcctgccaga 2221 cacagagggc cacacctacg tggcctttat tcgaatggag aaagatgatg acttcaccac 2281 ctggacccag cttgccaagt gcctccatat ctgggacctg gatgtgcgtg gcaaccatcg 2341 gggcctgtgg agattgtttc ggaagaagaa ccacttcctg gtggtggggg tcccggcttc 2401 cccctactca gtgaagaagc caccctcagt caccccaatt ttcctggagc cacccccaaa 2461 ggaggaggga gccccaggag ccccagaaca gacaTAGGAC CCAGCTTTCT TGTACAAAGT 2521 TGGCATTATA AGAAAGCATT GCTTATCAAT TTGTTGCAAC GAACAGGTCA CTATCAGTCA 2581 AAATAAAATC ATTATTTGCC ATCCAGCTGA TATCCCCTAT AGTGAGTCGT ATTACATGGT 2641 CATAGCTGTT TCCTGGCAGC TCTGGCCCGT GTCTCAAAAT CTCTGATGTT ACATTGCACA 2701 AGATAAAATA ATATCATCAT GAACAATAAA ACTGTCTGCT TACATAAACA GTAATACAAG 2761 GGGTGTTATG AGCCATATTC AACGGGAAAC GTCGAGGCCG CGATTAAATT CCAACATGGA 2821 TGCTGATTTA TATGGGTATA AATGGGCTCG CGATAATGTC GGGCAATCAG GTGCGACAAT 2881 CTATCGCTTG TATGGGAAGC CCGATGCGCC AGAGTTGTTT CTGAAACATG GCAAAGGTAG 2941 CGTTGCCAAT GATGTTACAG ATGAGATGGT CAGACTAAAC TGGCTGACGG AATTTATGCC 3001 TCTTCCGACC ATCAAGCATT TTATCCGTAC TCCTGATGAT GCATGGTTAC TCACCACTGC 3061 GATCCCCGGA AAAACAGCAT TCCAGGTATT AGAAGAATAT CCTGATTCAG GTGAAAATAT 3121 TGTTGATGCG CTGGCAGTGT TCCTGCGCCG GTTGCATTCG ATTCCTGTTT GTAATTGTCC 3181 TTTTAACAGC GATCGCGTAT TTCGTCTCGC TCAGGCGCAA TCACGAATGA ATAACGGTTT 3241 GGTTGATGCG AGTGATTTTG ATGACGAGCG TAATGGCTGG CCTGTTGAAC AAGTCTGGAA 3301 AGAAATGCAT AAACTTTTGC CATTCTCACC GGATTCAGTC GTCACTCATG GTGATTTCTC 3361 ACTTGATAAC CTTATTTTTG ACGAGGGGAA ATTAATAGGT TGTATTGATG TTGGACGAGT 3421 CGGAATCGCA GACCGATACC AGGATCTTGC CATCCTATGG AACTGCCTCG GTGAGTTTTC 3481 TCCTTCATTA CAGAAACGGC TTTTTCAAAA ATATGGTATT GATAATCCTG ATATGAATAA 3541 ATTGCAGTTT CATTTGATGC TCGATGAGTT TTTCTAATCA GAATTGGTTA ATTGGTTGTA 3601 ACACTGGCAG AGCATTACGC TGACTTGACG GGACGGCGCA AGCTCATGAC CAAAATCCCT 3661 TAACGTGAGT TACGCGTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC 3721 TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT 3781 ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG 3841 CTTCAGCAGA GCGCAGATAC CAAATACTGT TCTTCTAGTG TAGCCGTAGT TAGGCCACCA 3901 CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC 3961 TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA 4021 TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC 4081 GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA 4141 AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG 4201 GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG 4261 ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG 4321 CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTT //