LOCUS POMGNT1-pGEn1-DE 6385 bp DNA circular 22-JAN-2013 FEATURES Location/Qualifiers CDS 129..1108 /dnas_title="AmpR" /label=AmpR /vntifkey="4" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_feature 2977..3054 /note="TCM Sig Seq" misc_recomb complement(3114..3142) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_feature 3055..3078 /note="8xHis Tag" misc_feature 3085..3108 /note="StrepTag" misc_feature 5013..5554 /note="WPRE" misc_recomb 4975..4997 /label=attB2 /note="attB2 site" /note="recognized as attB2" misc_feature complement(5561..5775) /note="BGH pA" misc_feature complement(5863..5992) /note="3'-ITR" misc_feature 3143..3165 /note="TEV site" source 3166..4968 /dnas_title="POMGNT1-pGEn1-DEST" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:1063 IMAGE:3138971" /tissue_type="Placenta, choriocarcinoma" /clone_lib="NIH_MGC_21" /lab_host="DH10B-R" /note="Vector: pOTB7" gene 3166..4968 /dnas_title="POMGNT1" /gene="POMGNT1" /gene_synonym="FLJ20277" /gene_synonym="GnTI.2" /gene_synonym="MEB" /gene_synonym="MGAT1.2" /db_xref="GeneID:55624" /db_xref="HGNC:19139" /db_xref="MIM:606822" CDS 3166..4968 /dnas_title="protein O-linked mannose beta1,2-N-acetylglucosaminyltransferase" /gene="POMGNT1" /gene_synonym="FLJ20277" /gene_synonym="GnTI.2" /gene_synonym="MEB" /gene_synonym="MGAT1.2" /codon_start=1 /product="protein O-linked mannose beta1,2-N-acetylglucosaminyltransferase" /protein_id="AAH01471.1" /db_xref="GI:12655225" /db_xref="GeneID:55624" /db_xref="HGNC:19139" /db_xref="MIM:606822" /translation="MDDWKPSPLIKPFGARKKRSWYLTWKYKLTNQRALRRFCQTGAV LFLLVTVIVNIKLILDTRRAISEANEDPEPEQDYDEALGRLEPPRRRGSGPRRVLDVE VYSSRSKVYVAVDGTTVLEDEAREQGRGIHVIVLNQATGHVMAKRVFDTYSPHEDEAM VLFLNMVAPGRVLICTVKDEGSFHLKDTAKALLRSLGSQAGPALGWRDTWAFVGRKGG PVFGEKHSKSPALSSWGDPVLLKTDVPLSSAVEAECHWADTELNRRRRRFCSKVEGYG SVCSCKDPTPIEFSPDPLPDNKVLNVPVAVIAGNRPNYLYRMLRSLLSAQGVSPQMIT VFIDGYYEEPMDVVALFGLRGIQHTPISIKNARVSQHYKASLTATFNLFPEAKFAVVL EEDLDIAVDFFSFLSQSIHLLEEDDSLYCISAWNDQGYEHTAEDPALLYRVETMPGLG WVLRRSLYKEELEPKWPTPEKLWDWDMWMRMPEQRRGRECIIPDVSRSYHFGIVGLNM NGYFHEAYFKKHKFNTVPGVQLRNVDSLKKEAYEVEVHRLLSEAEVLDHSKNPCEDSF LPDTEGHTYVAFIRMEKDDDFTTWTQLAKCLHIWDLDVRGNHRGLWRLFRKKNHFLVV GVPASPYSVKKPPSVTPIFLEPPPKEEGAPGAPEQT" misc_feature 3166..4968 /note="POMGNT1 coding region" misc_difference 3737..3737 /dnas_title="POMGNT1" /gene="POMGNT1" /gene_synonym="FLJ20277" /gene_synonym="GnTI.2" /gene_synonym="MEB" /gene_synonym="MGAT1.2" /note="'T' in cDNA is 'A' in the human genome; amino acid difference: 'V' in cDNA, 'E' in the human genome. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." misc_difference 4855..4855 /dnas_title="POMGNT1" /gene="POMGNT1" /gene_synonym="FLJ20277" /gene_synonym="GnTI.2" /gene_synonym="MEB" /gene_synonym="MGAT1.2" /note="'G' in cDNA is 'A' in the human genome; amino acid difference: 'V' in cDNA, 'M' in the human genome. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." vector join(4983..6385,1..3132) /source="pGEn1-DEST" /type="Destination Vector" clone 3133..4982 /source="POMGNT1-Nterm-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCGCCATGA GACTGCTGAC AGCCCTGTTC 3001 GCCTACTTCA TCGTGGCCCT GATCCTGGCC TTCAGCGTGT CCGCCAAGAG CATGCACCAC 3061 CACCATCACC ATCATCACAG CGCCTGGTCC CACCCCCAGT TCGAGAAAGA ATTATCAACA 3121 AGTTTGTACA AAAAAGCAGG CTctgaaaac ttgtactttc aaggcgacac tcggcgagcc 3181 atcagtgaag ccaatgaaga cccagagcca gagcaagact atgatgaggc cctaggccgc 3241 ctggagcccc cacggcgcag aggcagtggt ccccggcggg tcctggacgt agaggtgtat 3301 tcaagtcgca gcaaagtata tgtggcagtg gatggcacca cggtgctgga ggatgaggcc 3361 cgggagcagg gccggggcat ccatgtcatt gtcctcaacc aggccacggg ccacgtgatg 3421 gcaaaacgtg tgtttgacac gtactcacct catgaggatg aggccatggt gctattcctc 3481 aacatggtag cgcccggccg agtgctcatc tgcactgtca aggatgaggg ctccttccac 3541 ctcaaggaca cagccaaggc tctgctgagg agcctgggca gccaggctgg ccctgccctg 3601 ggctggaggg acacatgggc cttcgtggga cgaaaaggag gtcctgtctt cggggagaaa 3661 cattctaaat cacctgccct ctcttcctgg ggggacccag tcctgctgaa gacagatgtg 3721 ccattgagct cagcagtaga ggcagagtgc cactgggcag acacagagct gaaccgtcgc 3781 cgccggcgct tctgcagcaa agttgagggc tatggaagtg tatgcagctg caaggacccc 3841 acacccatcg agttcagccc tgacccactc ccagacaaca aggtcctcaa tgtgcctgtg 3901 gctgtcattg cagggaaccg acccaattac ctgtacagga tgctgcgctc tctgctttca 3961 gcccaggggg tgtctcctca gatgataaca gttttcattg acggctacta tgaggaaccc 4021 atggatgtgg tggcactgtt tggtctgagg ggcatccagc atactcccat cagcatcaag 4081 aatgcccgcg tgtctcagca ctacaaggcc agcctcactg ccactttcaa cctgtttccg 4141 gaggccaagt ttgctgtggt tctggaagag gacctggaca ttgctgtgga ttttttcagt 4201 ttcctgagcc aatccatcca cctactggag gaggatgaca gcctgtactg catctctgcc 4261 tggaatgacc aggggtatga acacacggct gaggacccag cactactgta ccgtgtggag 4321 accatgcctg ggctgggctg ggtgctcagg aggtccttgt acaaggagga gcttgagccc 4381 aagtggccta caccggaaaa gctctgggat tgggacatgt ggatgcggat gcctgaacaa 4441 cgccggggcc gagagtgcat catccctgac gtttcccgat cctaccactt tggcatcgtc 4501 ggcctcaaca tgaatggcta ctttcacgag gcctacttca agaagcacaa gttcaacacg 4561 gttccaggtg tccagctcag gaatgtggac agtctgaaga aagaagctta tgaagtggaa 4621 gttcacaggc tgctcagtga ggctgaggtt ctggaccaca gcaagaaccc ttgtgaagac 4681 tctttcctgc cagacacaga gggccacacc tacgtggcct ttattcgaat ggagaaagat 4741 gatgacttca ccacctggac ccagcttgcc aagtgcctcc atatctggga cctggatgtg 4801 cgtggcaacc atcggggcct gtggagattg tttcggaaga agaaccactt cctggtggtg 4861 ggggtcccgg cttcccccta ctcagtgaag aagccaccct cagtcacccc aattttcctg 4921 gagccacccc caaaggagga gggagcccca ggagccccag aacagacaTA GGACCCAGCT 4981 TTCTTGTACA AAGTGGTTGA TAGCTTGGAT CCAATCAACC TCTGGATTAC AAAATTTGTG 5041 AAAGATTGAC TGGTATTCTT AACTATGTTG CTCCTTTTAC GCTATGTGGA TACGCTGCTT 5101 TAATGCCTTT GTATCATGCT ATTGCTTCCC GTATGGCTTT CATTTTCTCC TCCTTGTATA 5161 AATCCTGGTT GCTGTCTCTT TATGAGGAGT TGTGGCCCGT TGTCAGGCAA CGTGGCGTGG 5221 TGTGCACTGT GTTTGCTGAC GCAACCCCCA CTGGTTGGGG CATTGCCACC ACCTGTCAGC 5281 TCCTTTCCGG GACTTTCGCT TTCCCCCTCC CTATTGCCAC GGCGGAACTC ATCGCCGCCT 5341 GCCTTGCCCG CTGCTGGACA GGGGCTCGGC TGTTGGGCAC TGACAATTCC GTGGTGTTGT 5401 CGGGGAAGCT GACGTCCTTT CCATGGCTGC TCGCCTGTGT TGCCACCTGG ATTCTGCGCG 5461 GGACGTCCTT CTGCTACGTC CCTTCGGCCC TCAATCCAGC GGACCTTCCT TCCCGCGGCC 5521 TGCTGCCGGC TCTGCGGCCT CTTCCGCGTC TTCGAGATCT GCCTCGACTG TGCCTTCTAG 5581 TTGCCAGCCA TCTGTTGTTT GCCCCTCCCC CGTGCCTTCC TTGACCCTGG AAGGTGCCAC 5641 TCCCACTGTC CTTTCCTAAT AAAATGAGGA AATTGCATCG CATTGTCTGA GTAGGTGTCA 5701 TTCTATTCTG GGGGGTGGGG TGGGGCAGGA CAGCAAGGGG GAGGATTGGG AAGACAATAG 5761 CAGGCATGCT GGGGACTCGA GTTAAGGGCG AATTGCCGAT TAGGATCTTC CTAGAGCATG 5821 GCTACGTAGA TAAGTAGCAT GGCGGGTTAA TCATTAACTA CAAGGAACCC CTAGTGATGG 5881 AGTTGGCCAC TCCCTCTCTG CGCGCTCGCT CGCTCACTGA GGCCGGGCGA CCAAAGGTCG 5941 CCCGACGCCC GGGCTTTGCC CGGGCGGCCT CAGTGAGCGA GCGAGCGCGC AGCCTTAATT 6001 AAGAGCTCAT GGCGCGCCTA GGCCTTGACG GCCTTCCGCC AATTCGCCCT ATAGTGAGTC 6061 GTATTACGTC GCGCTCACTG GCCGTCGTTT TACAACGTCG TGACTGGGAA AACCCTGGCG 6121 TTACCCAACT TAATCGCCTT GCAGCACATC CCCCTTTCGC CAGCTGGCGT AATAGCGAAG 6181 AGGCCCGCAC CGAAACGCCC TTCCCAACAG TTGCGCAGCC TGAATGGCGA ATGGGAGCGC 6241 CCTGTAGCGG CCACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC 6301 GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTTAA 6361 CAAAATATTA ACGCTTACAA TTTAG //