LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(1823..1919) /label=attL2 /note="attL2 (c)" misc_signal complement(1934..1953) /note="T7 Promoter/priming site (c)" primer_bind 1961..1977 /note="M13 Reverse priming site" gene 2090..2899 /note="Kanamycin resistance gene" rep_origin 3020..3693 /note="pUC origin" vector join(1831..3696,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 669..691 /note="TEV site" source 692..1816 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:104075 IMAGE:30915526" /tissue_type="Lung, PCR rescued clones" /clone_lib="NIH_MGC_273" /lab_host="DH10B" /note="Vector: pCR4 Topo TA with reversed insert" gene 692..1816 /gene="GCNT2" /gene_synonym=bA360O19.2 /gene_synonym=bA421M1.1 /gene_synonym=GCNT2C /gene_synonym=IGNT /gene_synonym=NAGCT1 /gene_synonym=ULG3 /db_xref="GeneID:2651" /db_xref="HGNC:4204" /db_xref="MIM:600429" misc_feature 692..1816 /gene="GCNT2" /gene_synonym=bA360O19.2 /gene_synonym=bA421M1.1 /gene_synonym=GCNT2C /gene_synonym=IGNT /gene_synonym=NAGCT1 /gene_synonym=ULG3 /note="; This cDNA sequence aligns to the human reference genome (build 36) without differences" CDS 692..1816 /dnas_title="glucosaminyl (N-acetyl) transferase 2, I-branching enzyme, isoform B" /gene="GCNT2" /gene_synonym=bA360O19.2 /gene_synonym=bA421M1.1 /gene_synonym=GCNT2C /gene_synonym=IGNT /gene_synonym=NAGCT1 /gene_synonym=ULG3 /codon_start=1 /product="glucosaminyl (N-acetyl) transferase 2, I-branching enzyme, isoform B" /protein_id="AAH74801.1" /db_xref="GI:50960071" /db_xref="GeneID:2651" /db_xref="HGNC:4204" /db_xref="MIM:600429" /translation="MPLSMRYLFIISVSSVIIFIVFSVFNFGGDPSFQRLNISDPLRL TQVCTSFINGKTRFLWKNKLMIHEKSSCKEYLTQSHYITAPLSKEEADFPLAYIMVIH HHFDTFARLFRAIYMPQNIYCVHVDEKATTEFKDAVEQLLSCFPNAFLASKMEPVVYG GISRLQADLNCIRDLSAFEVSWKYVINTCGQDFPLKTNKEIVQYLKGFKGKNITPGVL PPAHAIGRTKYVHQEHLGKELSYVIRTTALKPPPPHNLTIYFGSAYVALSREFANFVL HDPRAVDLLQWSKDTFSPDEHFWVTLNRIPGVPGSMPNASWTGNLRAIKWSDMEDRHG GCHGHYVHGICIYGNGDLKWLVNSPSLFANKFELNTYPLTVECLELRHRERTLNQSET AIQPSWYF" misc_feature 692..1816 /note="GCNT2 coding region" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg caattttggg ggagatccaa gcttccaaag 721 gctaaatatc tcagaccctt tgaggctgac tcaagtttgc acatctttta tcaatggaaa 781 aacacgtttc ctgtggaaaa acaaactaat gatccatgag aagtcttctt gcaaggaata 841 cttgacccag agccactaca tcacagcccc tttatctaag gaagaagctg actttccctt 901 ggcatatata atggtcatcc atcatcactt tgacaccttt gcaaggctct tcagggctat 961 ttacatgccc caaaatatct actgtgttca tgtggatgaa aaagcaacaa ctgaatttaa 1021 agatgcggta gagcaactat taagctgctt cccaaacgct tttctggctt ccaagatgga 1081 acccgttgtc tatggaggga tctccaggct ccaggctgac ctgaactgca tcagagatct 1141 ttctgccttc gaggtctcat ggaagtacgt tatcaacacc tgtgggcaag acttccccct 1201 gaaaaccaac aaggaaatag ttcagtatct gaaaggattt aaaggtaaaa atatcacccc 1261 aggggtgctg cccccagctc atgcaattgg acggactaaa tatgtccacc aagagcacct 1321 gggcaaagag ctttcctatg tgataagaac aacagcgttg aaaccgcctc ccccccataa 1381 tctcacaatt tactttggct ctgcctatgt ggctctatca agagagtttg ccaactttgt 1441 tctgcatgac ccacgggctg ttgatttgct ccagtggtcc aaggacactt tcagtcctga 1501 tgagcatttc tgggtgacac tcaataggat tccaggtgtt cctggctcta tgccaaatgc 1561 atcctggact ggaaacctca gagctataaa gtggagtgac atggaagaca gacacggagg 1621 ctgccacggc cactatgtac atggtatttg tatctatgga aacggagact taaagtggct 1681 ggttaattca ccaagcctgt ttgctaacaa gtttgagctt aatacctacc cccttactgt 1741 ggaatgccta gaactgaggc atcgcgaaag aaccctcaat cagagtgaaa ctgcgataca 1801 acccagctgg tattttTAGG ACCCAGCTTT CTTGTACAAA GTTGGCATTA TAAGAAAGCA 1861 TTGCTTATCA ATTTGTTGCA ACGAACAGGT CACTATCAGT CAAAATAAAA TCATTATTTG 1921 CCATCCAGCT GATATCCCCT ATAGTGAGTC GTATTACATG GTCATAGCTG TTTCCTGGCA 1981 GCTCTGGCCC GTGTCTCAAA ATCTCTGATG TTACATTGCA CAAGATAAAA TAATATCATC 2041 ATGAACAATA AAACTGTCTG CTTACATAAA CAGTAATACA AGGGGTGTTA TGAGCCATAT 2101 TCAACGGGAA ACGTCGAGGC CGCGATTAAA TTCCAACATG GATGCTGATT TATATGGGTA 2161 TAAATGGGCT CGCGATAATG TCGGGCAATC AGGTGCGACA ATCTATCGCT TGTATGGGAA 2221 GCCCGATGCG CCAGAGTTGT TTCTGAAACA TGGCAAAGGT AGCGTTGCCA ATGATGTTAC 2281 AGATGAGATG GTCAGACTAA ACTGGCTGAC GGAATTTATG CCTCTTCCGA CCATCAAGCA 2341 TTTTATCCGT ACTCCTGATG ATGCATGGTT ACTCACCACT GCGATCCCCG GAAAAACAGC 2401 ATTCCAGGTA TTAGAAGAAT ATCCTGATTC AGGTGAAAAT ATTGTTGATG CGCTGGCAGT 2461 GTTCCTGCGC CGGTTGCATT CGATTCCTGT TTGTAATTGT CCTTTTAACA GCGATCGCGT 2521 ATTTCGTCTC GCTCAGGCGC AATCACGAAT GAATAACGGT TTGGTTGATG CGAGTGATTT 2581 TGATGACGAG CGTAATGGCT GGCCTGTTGA ACAAGTCTGG AAAGAAATGC ATAAACTTTT 2641 GCCATTCTCA CCGGATTCAG TCGTCACTCA TGGTGATTTC TCACTTGATA ACCTTATTTT 2701 TGACGAGGGG AAATTAATAG GTTGTATTGA TGTTGGACGA GTCGGAATCG CAGACCGATA 2761 CCAGGATCTT GCCATCCTAT GGAACTGCCT CGGTGAGTTT TCTCCTTCAT TACAGAAACG 2821 GCTTTTTCAA AAATATGGTA TTGATAATCC TGATATGAAT AAATTGCAGT TTCATTTGAT 2881 GCTCGATGAG TTTTTCTAAT CAGAATTGGT TAATTGGTTG TAACACTGGC AGAGCATTAC 2941 GCTGACTTGA CGGGACGGCG CAAGCTCATG ACCAAAATCC CTTAACGTGA GTTACGCGTC 3001 GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT 3061 TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT 3121 GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT 3181 ACCAAATACT GTTCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC 3241 ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA 3301 GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG 3361 CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG 3421 ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG 3481 GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA 3541 CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT 3601 GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG 3661 GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTT //