LOCUS GCNT4-pGEn1-DEST 5839 bp DNA circular 22-JAN-2013 FEATURES Location/Qualifiers CDS 129..1108 /dnas_title="AmpR" /label=AmpR /vntifkey="4" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_feature 2977..3054 /note="TCM Sig Seq" misc_recomb complement(3114..3142) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_feature 3055..3078 /note="8xHis Tag" misc_feature 3085..3108 /note="StrepTag" misc_feature 4467..5008 /note="WPRE" misc_recomb 4429..4451 /label=attB2 /note="attB2 site" /note="recognized as attB2" misc_feature complement(5015..5229) /note="BGH pA" misc_feature complement(5317..5446) /note="3'-ITR" misc_feature 3143..3165 /note="TEV site" source 3166..4422 /dnas_title="GCNT4-pGEn1-DEST" /organism="synthetic construct" /mol_type="other DNA" /db_xref="taxon:32630" /clone="MGC:193185 IMAGE:100064006" /clone_lib="NIH_MGC_435" /lab_host="DH10B" /focus /note="Vector: pENTR223.1 with stop codon" source 3166..4422 /organism="Homo sapiens" /mol_type="other DNA" /db_xref="taxon:9606" gene 3166..4422 /dnas_title="GCNT4" /gene="GCNT4" /gene_synonym="C2GNT3" /db_xref="GeneID:51301" /db_xref="HGNC:17973" CDS 3166..4422 /dnas_title="glucosaminyl (N-acetyl) transferase 4, core 2 (beta-1,6-N-acetylglucosaminyltransferase)" /gene="GCNT4" /gene_synonym="C2GNT3" /codon_start=1 /product="glucosaminyl (N-acetyl) transferase 4, core 2 (beta-1,6-N-acetylglucosaminyltransferase)" /protein_id="AAI60070.1" /db_xref="GI:182888315" /db_xref="GeneID:51301" /db_xref="HGNC:17973" /translation="MKIFKCYFKHTLQQKVFILFLTLWLLSLLKLLNVRRLFPQKDIY LVEYSLSTSPFVRNRYTHVKDEVRYEVNCSGIYEQEPLEIGKSLEIRRRDIIDLEDDD VVAMTSDCDIYQTLRGYAQKLVSKEEKSFPIAYSLVVHKDAIMVERLIHAIYNQHNIY CIHYDRKAPDTFKVAMNNLAKCFSNIFIASKLEAVEYAHISRLQADLNCLSDLLKSSI QWKYVINLCGQDFPLKSNFELVSELKKLNGANMLETVKPPNSKLERFTYHHELRRVPY EYVKLPIRTNISKEAPPHNIQIFVGSAYFVLSQAFVKYIFNNSIVQDFFAWSKDTYSP DEHFWATLIRVPGIPGEISRSAQDVSDLQSKTRLVKWNYYEGFFYPSCTGSHLRSVCI YGAAELRWLIKDGHWFANKFDSKVDPILIKCLAEKLEEQQRDWITLPSEKLFMDRNLT TTS" misc_feature 3166..4422 /note="GCNT4 coding region" vector join(4437..5839,1..3132) /source="pGEn1-DEST" /type="Destination Vector" clone 3133..4436 /source="GCNT4-Nterm-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCGCCATGA GACTGCTGAC AGCCCTGTTC 3001 GCCTACTTCA TCGTGGCCCT GATCCTGGCC TTCAGCGTGT CCGCCAAGAG CATGCACCAC 3061 CACCATCACC ATCATCACAG CGCCTGGTCC CACCCCCAGT TCGAGAAAGA ATTATCAACA 3121 AGTTTGTACA AAAAAGCAGG CTctgaaaac ttgtactttc aaggcagacg actctttccg 3181 caaaaagaca tttacttggt tgagtactcc ctaagtacct cgccttttgt aagaaacaga 3241 tacactcatg ttaaggatga agtcaggtat gaagttaact gttcgggtat ctatgaacag 3301 gagcctttgg aaattggaaa gagtctggaa ataagaagaa gggacatcat tgacttggag 3361 gatgatgatg ttgtggcaat gaccagtgat tgtgacattt atcagactct aagaggttat 3421 gctcaaaagc ttgtctcaaa ggaggagaaa agcttcccaa tagcctattc tttggttgtc 3481 cacaaagatg caattatggt tgaaaggctt atccatgcta tatacaacca gcacaatatt 3541 tactgcatcc attatgatcg taaggcacct gataccttca aagttgccat gaacaattta 3601 gctaagtgct tctccaatat tttcattgct tccaaattag aggctgtgga atatgcccac 3661 atttccagac tccaggctga tttaaattgc ttgtcggacc ttctgaagtc ttcaatccag 3721 tggaaatatg ttatcaactt gtgtgggcaa gattttcccc tgaagtcaaa ttttgaattg 3781 gtgtcagagt tgaaaaaact caatggagca aatatgttgg agacggtgaa acccccaaac 3841 agtaaattgg aaagattcac ttaccatcat gaacttagac gggtgcctta tgaatatgtg 3901 aagctaccaa taaggacaaa catctccaag gaagcacccc cccataacat tcagatattt 3961 gttggcagtg cttattttgt tttaagtcaa gcatttgtta aatatatttt caacaactcc 4021 atcgttcaag acttttttgc ctggtctaaa gacacatact ctcctgatga gcacttttgg 4081 gctaccttga ttcgggttcc aggaatacct ggggagattt ccagatcagc ccaggatgtg 4141 tctgatctgc agagtaagac tcgccttgtc aagtggaatt actatgaagg ctttttctat 4201 cccagttgta ctggatctca ccttcgaagc gtgtgtattt atggagctgc agaattaagg 4261 tggcttatca aagatggaca ttggtttgct aataaatttg attctaaggt ggaccctatc 4321 ttgattaaat gcttggcaga aaagcttgaa gaacagcaga gagactggat cactttgccc 4381 tcagaaaagt tatttatgga tagaaatctc actaccacat caTAGGACCC AGCTTTCTTG 4441 TACAAAGTGG TTGATAGCTT GGATCCAATC AACCTCTGGA TTACAAAATT TGTGAAAGAT 4501 TGACTGGTAT TCTTAACTAT GTTGCTCCTT TTACGCTATG TGGATACGCT GCTTTAATGC 4561 CTTTGTATCA TGCTATTGCT TCCCGTATGG CTTTCATTTT CTCCTCCTTG TATAAATCCT 4621 GGTTGCTGTC TCTTTATGAG GAGTTGTGGC CCGTTGTCAG GCAACGTGGC GTGGTGTGCA 4681 CTGTGTTTGC TGACGCAACC CCCACTGGTT GGGGCATTGC CACCACCTGT CAGCTCCTTT 4741 CCGGGACTTT CGCTTTCCCC CTCCCTATTG CCACGGCGGA ACTCATCGCC GCCTGCCTTG 4801 CCCGCTGCTG GACAGGGGCT CGGCTGTTGG GCACTGACAA TTCCGTGGTG TTGTCGGGGA 4861 AGCTGACGTC CTTTCCATGG CTGCTCGCCT GTGTTGCCAC CTGGATTCTG CGCGGGACGT 4921 CCTTCTGCTA CGTCCCTTCG GCCCTCAATC CAGCGGACCT TCCTTCCCGC GGCCTGCTGC 4981 CGGCTCTGCG GCCTCTTCCG CGTCTTCGAG ATCTGCCTCG ACTGTGCCTT CTAGTTGCCA 5041 GCCATCTGTT GTTTGCCCCT CCCCCGTGCC TTCCTTGACC CTGGAAGGTG CCACTCCCAC 5101 TGTCCTTTCC TAATAAAATG AGGAAATTGC ATCGCATTGT CTGAGTAGGT GTCATTCTAT 5161 TCTGGGGGGT GGGGTGGGGC AGGACAGCAA GGGGGAGGAT TGGGAAGACA ATAGCAGGCA 5221 TGCTGGGGAC TCGAGTTAAG GGCGAATTGC CGATTAGGAT CTTCCTAGAG CATGGCTACG 5281 TAGATAAGTA GCATGGCGGG TTAATCATTA ACTACAAGGA ACCCCTAGTG ATGGAGTTGG 5341 CCACTCCCTC TCTGCGCGCT CGCTCGCTCA CTGAGGCCGG GCGACCAAAG GTCGCCCGAC 5401 GCCCGGGCTT TGCCCGGGCG GCCTCAGTGA GCGAGCGAGC GCGCAGCCTT AATTAAGAGC 5461 TCATGGCGCG CCTAGGCCTT GACGGCCTTC CGCCAATTCG CCCTATAGTG AGTCGTATTA 5521 CGTCGCGCTC ACTGGCCGTC GTTTTACAAC GTCGTGACTG GGAAAACCCT GGCGTTACCC 5581 AACTTAATCG CCTTGCAGCA CATCCCCCTT TCGCCAGCTG GCGTAATAGC GAAGAGGCCC 5641 GCACCGAAAC GCCCTTCCCA ACAGTTGCGC AGCCTGAATG GCGAATGGGA GCGCCCTGTA 5701 GCGGCCACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC 5761 GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT 5821 ATTAACGCTT ACAATTTAG //