LOCUS XYLT2-pGEn1-DEST 7063 bp DNA circular 22-JAN-2013 FEATURES Location/Qualifiers CDS 129..1108 /dnas_title="AmpR" /label=AmpR /vntifkey="4" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_feature 2977..3054 /note="TCM Sig Seq" misc_recomb complement(3114..3142) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_feature 3055..3078 /note="8xHis Tag" misc_feature 3085..3108 /note="StrepTag" misc_feature 5691..6232 /note="WPRE" misc_recomb 5653..5675 /label=attB2 /note="attB2 site" /note="recognized as attB2" misc_feature complement(6239..6453) /note="BGH pA" misc_feature complement(6541..6670) /note="3'-ITR" misc_feature 3143..3165 /note="TEV site" source 3166..5646 /dnas_title="XYLT2-pGEn1-DEST" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:59736 IMAGE:6380013" /tissue_type="Pancreas, epithelioid carcinoma" /clone_lib="NIH_MGC_42" /lab_host="DH10B-R" /note="Vector: pOTB7" gene 3166..5646 /dnas_title="XYLT2" /gene="XYLT2" /gene_synonym="XT-II" /gene_synonym="XT2" /gene_synonym="xylT-II" /db_xref="GeneID:64132" /db_xref="HGNC:15517" /db_xref="MIM:608125" CDS 3166..5646 /dnas_title="xylosyltransferase II" /gene="XYLT2" /gene_synonym="XT-II" /gene_synonym="XT2" /gene_synonym="xylT-II" /codon_start=1 /product="xylosyltransferase II" /protein_id="AAH52262.2" /db_xref="GI:127798045" /db_xref="GeneID:64132" /db_xref="HGNC:15517" /db_xref="MIM:608125" /translation="MVASARVQKLVRRYKLAIATALAILLLQGLVVWSFSGLEEDEAG EKGRQRKPRPLDPGEGSKDTDSSAGRRGSTGRRHGRWRGRAESPGVPVAKVVRAVTSR QRASRRVPPAPPPEAPGRQNLSGAAAGEALVGAAGFPPHGDTGSVEGAPQPTDNGFTP KCEIVGKDALSALARASTKQCQQEIANVVCLHQAGSLMPKAVPRHCQLTGKMSPGIQW DESQAQQPMDGPPVRIAYMLVVHGRAIRQLKRLLKAVYHEQHFFYIHVDKRSDYLHRE VVELAQGYDNVRVTPWRMVTIWGGASLLTMYLRSMRDLLEVPGWAWDFFINLSATDYP TRTNEELVAFLSKNRDKNFLKSHGRDNSRFIKKQGLDRLFHECDSHMWRLGERQIPAG IVVDGGSDWFVLTRSFVEYVVYTDDPLVAQLRQFYTYTLLPAESFFHTVLENSLACET LVDNNLRVTNWNRKLGCKCQYKHIVDWCGCSPNDFKPQDFLRLQQVSRPTFFARKFES TVNQEVLEILDFHLYGSYPPGTPALKAYWENTYDAADGPSGLSDVMLTAYTAFARLSL HHAATAAPPMGTPLCRFEPRGLPSSVHLYFYDDHFQGYLVTQAVQPSAQGPAETLEMW LMPQGSLKLLGRSDQASRLQSLEVGTDWDPKERLFRNFGGLLGPLDEPVAVQRWARGP NLTATVVWIDPTYVVATSYDITVDTETEVTQYKPPLSRPLRPGPWTVRLLQFWEPLGE TRFLVLPLTFNRKLPLRKDDASWLHAGPPHNEYMEQSFQGLSSILNLPQPELAEEAAQ RHTQLTGPALEAWTDRELSSFWSVAGLCAIGPSPCPSLEPCRLTSWSSLSPDPKSELG PVKADGRLR" misc_difference 3228..3228 /dnas_title="XYLT2" /gene="XYLT2" /gene_synonym="XT-II" /gene_synonym="XT2" /gene_synonym="xylT-II" /note="'A' in cDNA is 'G' in the human genome; no amino acid change. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." misc_difference 3393..3393 /dnas_title="XYLT2" /gene="XYLT2" /gene_synonym="XT-II" /gene_synonym="XT2" /gene_synonym="xylT-II" /note="'T' in cDNA is 'C' in the human genome; no amino acid change. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." misc_feature 3166..5646 /note="XYLT2 coding region" misc_difference 3965..3965 /dnas_title="XYLT2" /gene="XYLT2" /gene_synonym="XT-II" /gene_synonym="XT2" /gene_synonym="xylT-II" /note="'C' in cDNA is 'G' in the human genome; amino acid difference: 'T' in cDNA, 'R' in the human genome." misc_difference 4620..4620 /dnas_title="XYLT2" /gene="XYLT2" /gene_synonym="XT-II" /gene_synonym="XT2" /gene_synonym="xylT-II" /note="'C' in cDNA is 'T' in the human genome; no amino acid change." vector join(5661..7063,1..3132) /source="pGEn1-DEST" /type="Destination Vector" clone 3133..5660 /source="XYLT2-Nterm-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCGCCATGA GACTGCTGAC AGCCCTGTTC 3001 GCCTACTTCA TCGTGGCCCT GATCCTGGCC TTCAGCGTGT CCGCCAAGAG CATGCACCAC 3061 CACCATCACC ATCATCACAG CGCCTGGTCC CACCCCCAGT TCGAGAAAGA ATTATCAACA 3121 AGTTTGTACA AAAAAGCAGG CTctgaaaac ttgtactttc aaggcgagga ggacgaggcg 3181 ggcgagaaag gaaggcagag gaagccacgg ccactggacc ctggcgaagg ttccaaggac 3241 acagacagtt cagcagggcg acggggcagc acaggcagaa ggcatgggcg ctggcggggc 3301 cgtgctgaga gcccaggagt gcccgtggcc aaggtggtac gggcagtaac cagccggcag 3361 agagccagcc ggcgggtccc acctgcccca cctccggaag ccccaggccg ccagaacctg 3421 agtggggcag cagctgggga ggcgctggta ggggcagctg gcttcccacc acacggagat 3481 acagggagcg tggagggcgc cccccagccc acggacaatg gcttcacccc caagtgcgag 3541 atcgtgggca aggacgcact gtctgcactg gcccgggcca gcaccaagca gtgccagcag 3601 gagatcgcca atgtggtgtg cctgcaccag gctgggagcc tcatgcccaa ggctgtgccc 3661 cggcactgtc agctgactgg gaagatgagc cccggcatcc agtgggatga gagccaagcc 3721 cagcagccca tggatggccc cccggtgcga atcgcctaca tgctggtggt tcacggccgc 3781 gccatccgcc agctgaagcg tctcctcaag gccgtttatc acgagcagca cttcttttac 3841 atccatgtgg acaagcgttc cgactacctg caccgggagg tggtggagct ggcccagggc 3901 tatgataacg tgcgggtgac gccctggcgc atggttacca tctggggcgg ggccagcctc 3961 ctgacgatgt acctgcggag catgcgggac ctgctagagg tgcctggctg ggcctgggac 4021 ttcttcatca acctcagtgc cactgactat ccaaccagga ccaatgagga gctggtggca 4081 ttcctatcca agaaccggga caagaatttc ctcaagtcac atggccggga caactccagg 4141 ttcatcaaga aacagggcct ggaccggctc ttccatgagt gcgactcaca catgtggcgc 4201 ctgggcgagc ggcagatccc agcaggcatt gtggtggatg gcggttctga ctggttcgtg 4261 ctgacacgca gctttgtgga gtatgtggtg tacacagatg acccgcttgt ggcccagctg 4321 cgccagttct acacatacac actgctccca gccgagtcct tcttccacac ggtgctggag 4381 aacagcctgg cctgtgagac cctcgtggac aacaacctgc gggtcaccaa ctggaaccgc 4441 aagctgggct gcaagtgcca gtacaagcac attgtggact ggtgtggctg ctcccccaac 4501 gacttcaagc cacaggactt cctccggctg cagcaagtct ccagacccac cttcttcgcc 4561 cggaagttcg agtcgactgt gaaccaggag gtgctggaaa tcctggactt ccacctgtac 4621 ggcagctacc cccccggcac gccagccctc aaggcctact gggagaacac ctacgacgcg 4681 gctgatggcc ccagtgggct cagtgatgtc atgctcactg cttacacagc cttcgcccgc 4741 ctcagcctgc accatgccgc cactgctgca cccccaatgg gcaccccact ctgcaggttt 4801 gagcccaggg gcttgccgtc cagcgtgcac ctgtatttct atgacgacca tttccagggc 4861 tacctggtga cgcaggcggt gcagccctca gcccaggggc cggcagagac gcttgagatg 4921 tggctgatgc cccaagggtc gctgaagctg ttggggcgca gtgaccaggc cagccggctc 4981 cagagtctgg aggttggcac tgattgggac cccaaagagc gtcttttccg gaactttggg 5041 gggttactgg ggccgctgga cgagcctgtg gccgtgcagc gctgggcccg gggccccaac 5101 ctcacagcca cagtggtctg gatcgaccca acctatgtgg tggccacatc ttatgacatc 5161 acagtagata cggagactga ggtcacgcaa tacaagcccc cactgagccg gcccctgcgg 5221 ccagggccct ggactgttcg actccttcag ttctgggaac cgctgggtga gacccgcttc 5281 cttgtgctgc ccttgacctt caaccgcaaa ctacctctca ggaaagatga tgccagctgg 5341 ctgcacgcag ggccacccca caacgagtac atggagcaga gtttccaggg cctgagtagc 5401 atcctgaacc tgcctcagcc ggagctcgcg gaggaggctg cccagcggca cacacagctc 5461 acaggccctg cgctcgaggc ctggacagac agggaactga gcagcttctg gtccgtggct 5521 ggactgtgtg ccataggccc ctctccctgc ccctccctgg agccctgcag actgaccagc 5581 tggagctctc tgtcccccga ccccaaatca gagctggggc ctgtcaaagc agacgggcga 5641 ctcaggTAGG ACCCAGCTTT CTTGTACAAA GTGGTTGATA GCTTGGATCC AATCAACCTC 5701 TGGATTACAA AATTTGTGAA AGATTGACTG GTATTCTTAA CTATGTTGCT CCTTTTACGC 5761 TATGTGGATA CGCTGCTTTA ATGCCTTTGT ATCATGCTAT TGCTTCCCGT ATGGCTTTCA 5821 TTTTCTCCTC CTTGTATAAA TCCTGGTTGC TGTCTCTTTA TGAGGAGTTG TGGCCCGTTG 5881 TCAGGCAACG TGGCGTGGTG TGCACTGTGT TTGCTGACGC AACCCCCACT GGTTGGGGCA 5941 TTGCCACCAC CTGTCAGCTC CTTTCCGGGA CTTTCGCTTT CCCCCTCCCT ATTGCCACGG 6001 CGGAACTCAT CGCCGCCTGC CTTGCCCGCT GCTGGACAGG GGCTCGGCTG TTGGGCACTG 6061 ACAATTCCGT GGTGTTGTCG GGGAAGCTGA CGTCCTTTCC ATGGCTGCTC GCCTGTGTTG 6121 CCACCTGGAT TCTGCGCGGG ACGTCCTTCT GCTACGTCCC TTCGGCCCTC AATCCAGCGG 6181 ACCTTCCTTC CCGCGGCCTG CTGCCGGCTC TGCGGCCTCT TCCGCGTCTT CGAGATCTGC 6241 CTCGACTGTG CCTTCTAGTT GCCAGCCATC TGTTGTTTGC CCCTCCCCCG TGCCTTCCTT 6301 GACCCTGGAA GGTGCCACTC CCACTGTCCT TTCCTAATAA AATGAGGAAA TTGCATCGCA 6361 TTGTCTGAGT AGGTGTCATT CTATTCTGGG GGGTGGGGTG GGGCAGGACA GCAAGGGGGA 6421 GGATTGGGAA GACAATAGCA GGCATGCTGG GGACTCGAGT TAAGGGCGAA TTGCCGATTA 6481 GGATCTTCCT AGAGCATGGC TACGTAGATA AGTAGCATGG CGGGTTAATC ATTAACTACA 6541 AGGAACCCCT AGTGATGGAG TTGGCCACTC CCTCTCTGCG CGCTCGCTCG CTCACTGAGG 6601 CCGGGCGACC AAAGGTCGCC CGACGCCCGG GCTTTGCCCG GGCGGCCTCA GTGAGCGAGC 6661 GAGCGCGCAG CCTTAATTAA GAGCTCATGG CGCGCCTAGG CCTTGACGGC CTTCCGCCAA 6721 TTCGCCCTAT AGTGAGTCGT ATTACGTCGC GCTCACTGGC CGTCGTTTTA CAACGTCGTG 6781 ACTGGGAAAA CCCTGGCGTT ACCCAACTTA ATCGCCTTGC AGCACATCCC CCTTTCGCCA 6841 GCTGGCGTAA TAGCGAAGAG GCCCGCACCG AAACGCCCTT CCCAACAGTT GCGCAGCCTG 6901 AATGGCGAAT GGGAGCGCCC TGTAGCGGCC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA 6961 TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA 7021 ATTTAACGCG AATTTTAACA AAATATTAAC GCTTACAATT TAG //