LOCUS XYLT2-pGEn2-DEST 7788 bp DNA circular 22-JAN-2013 FEATURES Location/Qualifiers CDS 129..1108 /dnas_title="AmpR" /label=AmpR /vntifkey="4" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_recomb complement(3839..3867) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_feature 6416..6957 /note="WPRE" misc_recomb 6378..6400 /label=attB2 /note="attB2 site" /note="recognized as attB2" misc_feature complement(6964..7178) /note="BGH pA" misc_feature complement(7266..7395) /note="3'-ITR" misc_feature 2982..3056 /note="TCM signal sequence" misc_feature 3060..3083 /note="His tag" misc_feature 3084..3134 /note="AviTag" misc_feature 3135..3830 /note="Superfolder GFP" misc_feature 2976..2981 /note="Kozak seq" misc_feature 3868..3890 /note="TEV site" source 3891..6371 /dnas_title="XYLT2-pGEn2-DEST" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:59736 IMAGE:6380013" /tissue_type="Pancreas, epithelioid carcinoma" /clone_lib="NIH_MGC_42" /lab_host="DH10B-R" /note="Vector: pOTB7" gene 3891..6371 /dnas_title="XYLT2" /gene="XYLT2" /gene_synonym="XT-II" /gene_synonym="XT2" /gene_synonym="xylT-II" /db_xref="GeneID:64132" /db_xref="HGNC:15517" /db_xref="MIM:608125" CDS 3891..6371 /dnas_title="xylosyltransferase II" /gene="XYLT2" /gene_synonym="XT-II" /gene_synonym="XT2" /gene_synonym="xylT-II" /codon_start=1 /product="xylosyltransferase II" /protein_id="AAH52262.2" /db_xref="GI:127798045" /db_xref="GeneID:64132" /db_xref="HGNC:15517" /db_xref="MIM:608125" /translation="MVASARVQKLVRRYKLAIATALAILLLQGLVVWSFSGLEEDEAG EKGRQRKPRPLDPGEGSKDTDSSAGRRGSTGRRHGRWRGRAESPGVPVAKVVRAVTSR QRASRRVPPAPPPEAPGRQNLSGAAAGEALVGAAGFPPHGDTGSVEGAPQPTDNGFTP KCEIVGKDALSALARASTKQCQQEIANVVCLHQAGSLMPKAVPRHCQLTGKMSPGIQW DESQAQQPMDGPPVRIAYMLVVHGRAIRQLKRLLKAVYHEQHFFYIHVDKRSDYLHRE VVELAQGYDNVRVTPWRMVTIWGGASLLTMYLRSMRDLLEVPGWAWDFFINLSATDYP TRTNEELVAFLSKNRDKNFLKSHGRDNSRFIKKQGLDRLFHECDSHMWRLGERQIPAG IVVDGGSDWFVLTRSFVEYVVYTDDPLVAQLRQFYTYTLLPAESFFHTVLENSLACET LVDNNLRVTNWNRKLGCKCQYKHIVDWCGCSPNDFKPQDFLRLQQVSRPTFFARKFES TVNQEVLEILDFHLYGSYPPGTPALKAYWENTYDAADGPSGLSDVMLTAYTAFARLSL HHAATAAPPMGTPLCRFEPRGLPSSVHLYFYDDHFQGYLVTQAVQPSAQGPAETLEMW LMPQGSLKLLGRSDQASRLQSLEVGTDWDPKERLFRNFGGLLGPLDEPVAVQRWARGP NLTATVVWIDPTYVVATSYDITVDTETEVTQYKPPLSRPLRPGPWTVRLLQFWEPLGE TRFLVLPLTFNRKLPLRKDDASWLHAGPPHNEYMEQSFQGLSSILNLPQPELAEEAAQ RHTQLTGPALEAWTDRELSSFWSVAGLCAIGPSPCPSLEPCRLTSWSSLSPDPKSELG PVKADGRLR" misc_difference 3953..3953 /dnas_title="XYLT2" /gene="XYLT2" /gene_synonym="XT-II" /gene_synonym="XT2" /gene_synonym="xylT-II" /note="'A' in cDNA is 'G' in the human genome; no amino acid change. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." misc_difference 4118..4118 /dnas_title="XYLT2" /gene="XYLT2" /gene_synonym="XT-II" /gene_synonym="XT2" /gene_synonym="xylT-II" /note="'T' in cDNA is 'C' in the human genome; no amino acid change. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." misc_feature 3891..6371 /note="XYLT2 coding region" misc_difference 4690..4690 /dnas_title="XYLT2" /gene="XYLT2" /gene_synonym="XT-II" /gene_synonym="XT2" /gene_synonym="xylT-II" /note="'C' in cDNA is 'G' in the human genome; amino acid difference: 'T' in cDNA, 'R' in the human genome." misc_difference 5345..5345 /dnas_title="XYLT2" /gene="XYLT2" /gene_synonym="XT-II" /gene_synonym="XT2" /gene_synonym="xylT-II" /note="'C' in cDNA is 'T' in the human genome; no amino acid change." vector join(6386..7788,1..3857) /source="pGEn2-DEST" /type="Destination Vector" clone 3858..6385 /source="XYLT2-Nterm-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAgcg gccgcGCCAC cATGAGACTG CTGACCGCCC 3001 TGTTCGCCTA CTTCATCGTG GCCCTGATCC TGGCCTTCAG CGTGTCCGCC AAGAGCATGC 3061 ACCACCACCA TCACCATCAT CACATGAGCG GCCTGAACGA CATCTTCGAG GCCCAGAAAA 3121 TCGAGTGGCA CGAGATGAGC AAGGGCGAGG AACTGTTCAC CGGCGTGGTG CCCATCCTGG 3181 TGGAACTGGA CGGCGACGTG AACGGCCACA AGTTCTCTGT GCGGGGCGAG GGCGAGGGGG 3241 ACGCCACAAA TGGCAAGCTG ACCCTGAAGT TCATCTGCAC CACCGGAAAG CTGCCCGTGC 3301 CCTGGCCTAC CCTGGTCACA ACCCTGACCT ACGGCGTGCA GTGCTTCAGC AGATACCCCG 3361 ACCACATGAA GCGGCACGAT TTCTTCAAGA GCGCCATGCC CGAGGGCTAC GTGCAAGAAC 3421 GGACCATCAG CTTCAAGGAC GACGGCACCT ACAAGACCAG AGCCGAAGTG AAGTTCGAGG 3481 GCGACACCCT GGTCAACCGG ATCGAGCTGA AGGGCATCGA CTTCAAAGAG GACGGCAACA 3541 TCCTGGGCCA CAAGCTCGAG TACAACTTCA ACAGCCACAA CGTGTACATC ACCGCCGACA 3601 AGCAGAAGAA CGGCATCAAG GCCAACTTCA AGATCCGGCA CAACGTGGAA GATGGCAGCG 3661 TGCAGCTGGC CGACCACTAC CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC 3721 CCGACAATCA CTACCTGAGC ACCCAGAGCG TGCTGAGCAA GGACCCCAAC GAGAAGCGGG 3781 ACCACATGGT GCTGCTGGAA TTTGTGACCG CCGCTGGCAT CACCCACGGC GAATTCgcAT 3841 CAACAAGTTT GTACAAAAAA GCAGGCTctg aaaacttgta ctttcaaggc gaggaggacg 3901 aggcgggcga gaaaggaagg cagaggaagc cacggccact ggaccctggc gaaggttcca 3961 aggacacaga cagttcagca gggcgacggg gcagcacagg cagaaggcat gggcgctggc 4021 ggggccgtgc tgagagccca ggagtgcccg tggccaaggt ggtacgggca gtaaccagcc 4081 ggcagagagc cagccggcgg gtcccacctg ccccacctcc ggaagcccca ggccgccaga 4141 acctgagtgg ggcagcagct ggggaggcgc tggtaggggc agctggcttc ccaccacacg 4201 gagatacagg gagcgtggag ggcgcccccc agcccacgga caatggcttc acccccaagt 4261 gcgagatcgt gggcaaggac gcactgtctg cactggcccg ggccagcacc aagcagtgcc 4321 agcaggagat cgccaatgtg gtgtgcctgc accaggctgg gagcctcatg cccaaggctg 4381 tgccccggca ctgtcagctg actgggaaga tgagccccgg catccagtgg gatgagagcc 4441 aagcccagca gcccatggat ggccccccgg tgcgaatcgc ctacatgctg gtggttcacg 4501 gccgcgccat ccgccagctg aagcgtctcc tcaaggccgt ttatcacgag cagcacttct 4561 tttacatcca tgtggacaag cgttccgact acctgcaccg ggaggtggtg gagctggccc 4621 agggctatga taacgtgcgg gtgacgccct ggcgcatggt taccatctgg ggcggggcca 4681 gcctcctgac gatgtacctg cggagcatgc gggacctgct agaggtgcct ggctgggcct 4741 gggacttctt catcaacctc agtgccactg actatccaac caggaccaat gaggagctgg 4801 tggcattcct atccaagaac cgggacaaga atttcctcaa gtcacatggc cgggacaact 4861 ccaggttcat caagaaacag ggcctggacc ggctcttcca tgagtgcgac tcacacatgt 4921 ggcgcctggg cgagcggcag atcccagcag gcattgtggt ggatggcggt tctgactggt 4981 tcgtgctgac acgcagcttt gtggagtatg tggtgtacac agatgacccg cttgtggccc 5041 agctgcgcca gttctacaca tacacactgc tcccagccga gtccttcttc cacacggtgc 5101 tggagaacag cctggcctgt gagaccctcg tggacaacaa cctgcgggtc accaactgga 5161 accgcaagct gggctgcaag tgccagtaca agcacattgt ggactggtgt ggctgctccc 5221 ccaacgactt caagccacag gacttcctcc ggctgcagca agtctccaga cccaccttct 5281 tcgcccggaa gttcgagtcg actgtgaacc aggaggtgct ggaaatcctg gacttccacc 5341 tgtacggcag ctaccccccc ggcacgccag ccctcaaggc ctactgggag aacacctacg 5401 acgcggctga tggccccagt gggctcagtg atgtcatgct cactgcttac acagccttcg 5461 cccgcctcag cctgcaccat gccgccactg ctgcaccccc aatgggcacc ccactctgca 5521 ggtttgagcc caggggcttg ccgtccagcg tgcacctgta tttctatgac gaccatttcc 5581 agggctacct ggtgacgcag gcggtgcagc cctcagccca ggggccggca gagacgcttg 5641 agatgtggct gatgccccaa gggtcgctga agctgttggg gcgcagtgac caggccagcc 5701 ggctccagag tctggaggtt ggcactgatt gggaccccaa agagcgtctt ttccggaact 5761 ttggggggtt actggggccg ctggacgagc ctgtggccgt gcagcgctgg gcccggggcc 5821 ccaacctcac agccacagtg gtctggatcg acccaaccta tgtggtggcc acatcttatg 5881 acatcacagt agatacggag actgaggtca cgcaatacaa gcccccactg agccggcccc 5941 tgcggccagg gccctggact gttcgactcc ttcagttctg ggaaccgctg ggtgagaccc 6001 gcttccttgt gctgcccttg accttcaacc gcaaactacc tctcaggaaa gatgatgcca 6061 gctggctgca cgcagggcca ccccacaacg agtacatgga gcagagtttc cagggcctga 6121 gtagcatcct gaacctgcct cagccggagc tcgcggagga ggctgcccag cggcacacac 6181 agctcacagg ccctgcgctc gaggcctgga cagacaggga actgagcagc ttctggtccg 6241 tggctggact gtgtgccata ggcccctctc cctgcccctc cctggagccc tgcagactga 6301 ccagctggag ctctctgtcc cccgacccca aatcagagct ggggcctgtc aaagcagacg 6361 ggcgactcag gTAGGACCCA GCTTTCTTGT ACAAAGTGGT TGATAGCTTG GATCCAATCA 6421 ACCTCTGGAT TACAAAATTT GTGAAAGATT GACTGGTATT CTTAACTATG TTGCTCCTTT 6481 TACGCTATGT GGATACGCTG CTTTAATGCC TTTGTATCAT GCTATTGCTT CCCGTATGGC 6541 TTTCATTTTC TCCTCCTTGT ATAAATCCTG GTTGCTGTCT CTTTATGAGG AGTTGTGGCC 6601 CGTTGTCAGG CAACGTGGCG TGGTGTGCAC TGTGTTTGCT GACGCAACCC CCACTGGTTG 6661 GGGCATTGCC ACCACCTGTC AGCTCCTTTC CGGGACTTTC GCTTTCCCCC TCCCTATTGC 6721 CACGGCGGAA CTCATCGCCG CCTGCCTTGC CCGCTGCTGG ACAGGGGCTC GGCTGTTGGG 6781 CACTGACAAT TCCGTGGTGT TGTCGGGGAA GCTGACGTCC TTTCCATGGC TGCTCGCCTG 6841 TGTTGCCACC TGGATTCTGC GCGGGACGTC CTTCTGCTAC GTCCCTTCGG CCCTCAATCC 6901 AGCGGACCTT CCTTCCCGCG GCCTGCTGCC GGCTCTGCGG CCTCTTCCGC GTCTTCGAGA 6961 TCTGCCTCGA CTGTGCCTTC TAGTTGCCAG CCATCTGTTG TTTGCCCCTC CCCCGTGCCT 7021 TCCTTGACCC TGGAAGGTGC CACTCCCACT GTCCTTTCCT AATAAAATGA GGAAATTGCA 7081 TCGCATTGTC TGAGTAGGTG TCATTCTATT CTGGGGGGTG GGGTGGGGCA GGACAGCAAG 7141 GGGGAGGATT GGGAAGACAA TAGCAGGCAT GCTGGGGACT CGAGTTAAGG GCGAATTGCC 7201 GATTAGGATC TTCCTAGAGC ATGGCTACGT AGATAAGTAG CATGGCGGGT TAATCATTAA 7261 CTACAAGGAA CCCCTAGTGA TGGAGTTGGC CACTCCCTCT CTGCGCGCTC GCTCGCTCAC 7321 TGAGGCCGGG CGACCAAAGG TCGCCCGACG CCCGGGCTTT GCCCGGGCGG CCTCAGTGAG 7381 CGAGCGAGCG CGCAGCCTTA ATTAAGAGCT CATGGCGCGC CTAGGCCTTG ACGGCCTTCC 7441 GCCAATTCGC CCTATAGTGA GTCGTATTAC GTCGCGCTCA CTGGCCGTCG TTTTACAACG 7501 TCGTGACTGG GAAAACCCTG GCGTTACCCA ACTTAATCGC CTTGCAGCAC ATCCCCCTTT 7561 CGCCAGCTGG CGTAATAGCG AAGAGGCCCG CACCGAAACG CCCTTCCCAA CAGTTGCGCA 7621 GCCTGAATGG CGAATGGGAG CGCCCTGTAG CGGCCACTCA ACCCTATCTC GGTCTATTCT 7681 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 7741 CAAAAATTTA ACGCGAATTT TAACAAAATA TTAACGCTTA CAATTTAG //