LOCUS MGAT5-pGEn2 7440 bp DNA circular 21-SEP-2016 FEATURES Location/Qualifiers CDS 129..1108 /dnas_title="AmpR" /label=AmpR /vntifkey="4" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_recomb complement(3839..3867) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_feature 6068..6609 /note="WPRE" misc_recomb 6030..6052 /label=attB2 /note="attB2 site" /note="recognized as attB2" misc_feature complement(6616..6830) /note="BGH pA" misc_feature complement(6918..7047) /note="3'-ITR" misc_feature 2982..3056 /note="TCM signal sequence" misc_feature 3060..3083 /note="His tag" misc_feature 3084..3134 /note="AviTag" misc_feature 3135..3830 /note="Superfolder GFP" misc_feature 2976..2981 /note="Kozak seq" misc_feature 3868..3890 /note="TEV site" misc_feature 3891..6023 /note="OPTIMIZED MGAT5 coding region" vector join(6038..7440,1..3857) /source="pGEn2-DEST" /type="Destination Vector" clone 3858..6037 /source="MGAT5pdonr" /type="Entry Clone" source 1..7440 /dnas_title="MGAT5-pGEn2" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAgcg gccgcGCCAC cATGAGACTG CTGACCGCCC 3001 TGTTCGCCTA CTTCATCGTG GCCCTGATCC TGGCCTTCAG CGTGTCCGCC AAGAGCATGC 3061 ACCACCACCA TCACCATCAT CACATGAGCG GCCTGAACGA CATCTTCGAG GCCCAGAAAA 3121 TCGAGTGGCA CGAGATGAGC AAGGGCGAGG AACTGTTCAC CGGCGTGGTG CCCATCCTGG 3181 TGGAACTGGA CGGCGACGTG AACGGCCACA AGTTCTCTGT GCGGGGCGAG GGCGAGGGGG 3241 ACGCCACAAA TGGCAAGCTG ACCCTGAAGT TCATCTGCAC CACCGGAAAG CTGCCCGTGC 3301 CCTGGCCTAC CCTGGTCACA ACCCTGACCT ACGGCGTGCA GTGCTTCAGC AGATACCCCG 3361 ACCACATGAA GCGGCACGAT TTCTTCAAGA GCGCCATGCC CGAGGGCTAC GTGCAAGAAC 3421 GGACCATCAG CTTCAAGGAC GACGGCACCT ACAAGACCAG AGCCGAAGTG AAGTTCGAGG 3481 GCGACACCCT GGTCAACCGG ATCGAGCTGA AGGGCATCGA CTTCAAAGAG GACGGCAACA 3541 TCCTGGGCCA CAAGCTCGAG TACAACTTCA ACAGCCACAA CGTGTACATC ACCGCCGACA 3601 AGCAGAAGAA CGGCATCAAG GCCAACTTCA AGATCCGGCA CAACGTGGAA GATGGCAGCG 3661 TGCAGCTGGC CGACCACTAC CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC 3721 CCGACAATCA CTACCTGAGC ACCCAGAGCG TGCTGAGCAA GGACCCCAAC GAGAAGCGGG 3781 ACCACATGGT GCTGCTGGAA TTTGTGACCG CCGCTGGCAT CACCCACGGC GAATTCgcAT 3841 CAACAAGTTT GTACAAAAAA GCAGGCTctg aaaacttgta ctttcaaggc CACTTCACCA 3901 TCCAGCAGCG GACCCAGCCT GAGAGCAGCT CCATGCTGAG AGAGCAGATC CTGGACCTGA 3961 GCAAGCGGTA CATCAAGGCC CTGGCCGAAG AGAACCGGAA CGTGGTGGAT GGCCCTTATG 4021 CCGGCGTGAT GACCGCCTAC GACCTGAAGA AAACCCTGGC CGTGCTGCTG GACAACATCC 4081 TGCAGAGAAT CGGCAAGCTG GAAAGCAAGG TGGACAACCT GGTCGTGAAC GGCACCGGCA 4141 CCAACAGCAC CAATTCCACC ACAGCCGTGC CCAGCCTGGT GGCCCTGGAA AAGATCAACG 4201 TGGCCGACAT CATCAACGGC GCCCAGGAAA AATGCGTGCT GCCCCCCATG GACGGCTACC 4261 CTCACTGCGA GGGCAAGATC AAGTGGATGA AGGACATGTG GCGGAGCGAC CCCTGCTACG 4321 CCGATTATGG CGTGGACGGC TCTACCTGCA GCTTCTTCAT CTACCTGAGC GAGGTGGAAA 4381 ACTGGTGCCC CCATCTGCCT TGGCGGGCCA AGAACCCTTA CGAGGAAGCC GACCACAACA 4441 GCCTGGCCGA GATCCGGACC GACTTCAATA TCCTGTACAG CATGATGAAG AAACACGAGG 4501 AATTCCGGTG GATGCGGCTG CGGATCAGAA GAATGGCCGA CGCCTGGATT CAGGCCATCA 4561 AGAGCCTGGC AGAGAAGCAG AATCTGGAAA AGCGCAAGCG GAAGAAAGTG CTGGTGCACC 4621 TGGGCCTGCT GACCAAAGAG AGCGGCTTCA AGATCGCCGA GACAGCCTTC TCTGGCGGCC 4681 CTCTGGGAGA ACTGGTGCAG TGGAGCGACC TGATCACCAG CCTGTACCTG CTGGGCCACG 4741 ACATCAGAAT CAGCGCCAGC CTGGCTGAGC TGAAAGAAAT CATGAAGAAA GTCGTGGGCA 4801 ATCGGAGCGG CTGCCCCACC GTGGGAGACA GAATCGTGGA ACTGATCTAC ATCGACATCG 4861 TGGGCCTGGC CCAGTTCAAA AAGACCCTGG GCCCTAGCTG GGTGCACTAC CAGTGCATGC 4921 TGCGGGTGCT GGACAGCTTC GGCACCGAGC CCGAGTTCAA CCACGCCAAC TACGCCCAGA 4981 GCAAGGGCCA CAAGACCCCC TGGGGCAAGT GGAACCTGAA CCCCCAGCAG TTCTACACCA 5041 TGTTCCCCCA TACCCCCGAC AACAGCTTCC TGGGCTTCGT GGTGGAACAG CACCTGAACA 5101 GCAGCGACAT TCACCACATC AACGAGATCA AGCGGCAGAA CCAGAGCCTG GTGTACGGCA 5161 AAGTGGACTC CTTCTGGAAG AACAAGAAGA TCTACCTGGA CATCATCCAC ACCTACATGG 5221 AAGTGCACGC CACCGTGTAC GGCTCCAGCA CCAAGAACAT CCCCAGCTAC GTGAAGAACC 5281 ACGGCATCCT GAGCGGCAGG GACCTGCAGT TCCTGCTGCG CGAGACAAAG CTGTTCGTGG 5341 GACTGGGCTT CCCCTACGAA GGACCCGCTC CCCTGGAAGC CATTGCCAAC GGCTGCGCCT 5401 TCCTGAACCC TAAGTTCAAC CCCCCCAAGA GCAGCAAGAA CACCGACTTT TTCATCGGAA 5461 AGCCCACCCT GCGCGAGCTG ACCAGCCAGC ACCCTTACGC CGAGGTGTTC ATCGGCAGAC 5521 CCCACGTGTG GACCGTGGAC CTGAACAACC AGGAAGAGGT GGAGGACGCC GTGAAGGCCA 5581 TCCTGAACCA GAAAATCGAG CCCTACATGC CCTACGAGTT TACCTGCGAG GGAATGCTGC 5641 AGCGGATCAA CGCCTTTATC GAGAAGCAGG ACTTCTGCCA CGGACAAGTG ATGTGGCCCC 5701 CTCTGAGCGC CCTGCAAGTG AAGCTGGCTG AACCTGGCCA GAGCTGCAAA CAAGTGTGCC 5761 AGGAAAGCCA GCTGATCTGC GAGCCTAGCT TCTTCCAGCA TCTGAACAAG GACAAGGACA 5821 TGCTGAAGTA CAAAGTGACC TGCCAGAGCA GCGAGCTGGC CAAGGACATC CTGGTGCCCA 5881 GCTTCGACCC CAAGAACAAG CACTGCGTGT TCCAGGGCGA CCTGCTGCTG TTTAGCTGTG 5941 CCGGCGCTCA CCCCAGACAC CAGAGAGTGT GCCCTTGCCG GGACTTCATC AAGGGCCAGG 6001 TGGCACTGTG CAAGGACTGT CTCTAGGACC CAGCTTTCTT GTACAAAGTG GTTGATAGCT 6061 TGGATCCAAT CAACCTCTGG ATTACAAAAT TTGTGAAAGA TTGACTGGTA TTCTTAACTA 6121 TGTTGCTCCT TTTACGCTAT GTGGATACGC TGCTTTAATG CCTTTGTATC ATGCTATTGC 6181 TTCCCGTATG GCTTTCATTT TCTCCTCCTT GTATAAATCC TGGTTGCTGT CTCTTTATGA 6241 GGAGTTGTGG CCCGTTGTCA GGCAACGTGG CGTGGTGTGC ACTGTGTTTG CTGACGCAAC 6301 CCCCACTGGT TGGGGCATTG CCACCACCTG TCAGCTCCTT TCCGGGACTT TCGCTTTCCC 6361 CCTCCCTATT GCCACGGCGG AACTCATCGC CGCCTGCCTT GCCCGCTGCT GGACAGGGGC 6421 TCGGCTGTTG GGCACTGACA ATTCCGTGGT GTTGTCGGGG AAGCTGACGT CCTTTCCATG 6481 GCTGCTCGCC TGTGTTGCCA CCTGGATTCT GCGCGGGACG TCCTTCTGCT ACGTCCCTTC 6541 GGCCCTCAAT CCAGCGGACC TTCCTTCCCG CGGCCTGCTG CCGGCTCTGC GGCCTCTTCC 6601 GCGTCTTCGA GATCTGCCTC GACTGTGCCT TCTAGTTGCC AGCCATCTGT TGTTTGCCCC 6661 TCCCCCGTGC CTTCCTTGAC CCTGGAAGGT GCCACTCCCA CTGTCCTTTC CTAATAAAAT 6721 GAGGAAATTG CATCGCATTG TCTGAGTAGG TGTCATTCTA TTCTGGGGGG TGGGGTGGGG 6781 CAGGACAGCA AGGGGGAGGA TTGGGAAGAC AATAGCAGGC ATGCTGGGGA CTCGAGTTAA 6841 GGGCGAATTG CCGATTAGGA TCTTCCTAGA GCATGGCTAC GTAGATAAGT AGCATGGCGG 6901 GTTAATCATT AACTACAAGG AACCCCTAGT GATGGAGTTG GCCACTCCCT CTCTGCGCGC 6961 TCGCTCGCTC ACTGAGGCCG GGCGACCAAA GGTCGCCCGA CGCCCGGGCT TTGCCCGGGC 7021 GGCCTCAGTG AGCGAGCGAG CGCGCAGCCT TAATTAAGAG CTCATGGCGC GCCTAGGCCT 7081 TGACGGCCTT CCGCCAATTC GCCCTATAGT GAGTCGTATT ACGTCGCGCT CACTGGCCGT 7141 CGTTTTACAA CGTCGTGACT GGGAAAACCC TGGCGTTACC CAACTTAATC GCCTTGCAGC 7201 ACATCCCCCT TTCGCCAGCT GGCGTAATAG CGAAGAGGCC CGCACCGAAA CGCCCTTCCC 7261 AACAGTTGCG CAGCCTGAAT GGCGAATGGG AGCGCCCTGT AGCGGCCACT CAACCCTATC 7321 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 7381 GAGCTGATTT AACAAAAATT TAACGCGAAT TTTAACAAAA TATTAACGCT TACAATTTAG //