LOCUS ALG9-pGEc2-DEST 7075 bp DNA circular 22-JAN-2013 FEATURES Location/Qualifiers misc_feature complement(6553..6682) /note="3'-ITR" misc_feature complement(6251..6465) /note="BGH pA" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" CDS 129..989 /dnas_title="AmpR" /label=AmpR /vntifkey="4" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_feature 5703..6244 /note="WPRE" misc_recomb complement(2974..3001) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_feature 4882..4904 /note="attB2" misc_feature 5682..5687 /note="BstB1 site" misc_feature 4905..5600 /note="Super GFP" misc_feature 5601..5651 /note="AviTag" misc_feature 5652..5675 /note="His Tag" PCR_frame 3002..4882 /note="Reading Frame" /set="Set 1" source 3006..4859 /dnas_title="ALG9-pGEc2-DEST" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:4563 IMAGE:3029372" /tissue_type="Muscle, rhabdomyosarcoma" /clone_lib="NIH_MGC_17" /lab_host="DH10B-R" /note="Vector: pOTB7" gene 3006..4859 /dnas_title="ALG9" /gene="ALG9" /gene_synonym="LOH11CR1J" /db_xref="GeneID:79796" /db_xref="HGNC:15672" /db_xref="MIM:606941" CDS 3006..4859 /dnas_title="asparagine-linked glycosylation 9, alpha-1,2-mannosyltransferase homolog (S. cerevisiae)" /gene="ALG9" /gene_synonym="LOH11CR1J" /codon_start=1 /product="asparagine-linked glycosylation 9, alpha-1,2-mannosyltransferase homolog (S. cerevisiae)" /protein_id="AAH09255.1" /db_xref="GI:14328092" /db_xref="GeneID:79796" /db_xref="HGNC:15672" /db_xref="MIM:606941" /translation="MASRGARQRLKGSGASSGDTAPAADKLRELLGSREAGGAEHRTE LSGNKAGQVWAPEGSTAFKCLLSARLCAALLSNISDCDETFNYWEPTHYLIYGEGFQT WEYSPAYAIRSYAYLLLHAWPAAFHARILQTNKILVFYFLRCLLAFVSCICELYFYKA VCKKFGLHVSRMMLAFLVLSTGMFCSSSAFLPSSFCMYTTLIAMTGWYMDKTSIAVLG VAAGAILGWPFSAALGLPIAFDLLVMKHRWKSFFHWSLMALILFLVPVVVIDSYYYGK LVIAPLNIVLYNVFTPHGPDLYGTEPWYFYLINGFLNFNVAFALALLVLPLTSLMEYL LQRFHVQNLGHPYWLTLAPMYIWFIIFFIQPHKEERFLFPVYPLICLCGAVALSALQH SFLYFQKCYHFVFQRYRLEHYTVTSNWLALGTVFLFGLLSFSRSVALFRGYHGPLDLY PEFYRIATDPTIHTVPEGRPVNVCVGKEWYRFPSSFLLPDNWQLQFIPSEFRGQLPKP FAEGPLATRIVPTDMNDQNLEEPSRYIDISKCHYLVDLDTMRETPREPKYSSNKEEWI SLAYRPFLDASRSSKLLRAFYVPFLSDQYTVYVNYTILKPRKAKQIRKKSGG" misc_difference 3065..3066 /dnas_title="ALG9" /gene="ALG9" /gene_synonym="LOH11CR1J" /note="1 base in the human genome, C, is not found in cDNA." misc_feature 3006..4859 /note="ALG9 coding region" misc_feature 4860..4880 /note="TEV site" PCR_primer 2992..3001 /dnas_title="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /primer="CAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" PCR_primer complement(3002..4889) /dnas_title="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original="ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG" PCR_product complement(2992..4889) /pair="Pair 1" /set="Set 1" /current=1 /score="1.000" vector join(4890..7075,1..2991) /source="pGEc2-DEST" /type="Destination Vector" clone 2992..4889 /source="ALG9-C-term-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCATCACAA GTTTGTACAA AAAAGCAGGC 3001 TCGCCatggc tagtcgaggg gctcggcagc gcctgaaggg cagcggggcc agcagtgggg 3061 atacggcccc ggctgcggac aagctgcggg agctgctggg cagccgagag gcgggcggcg 3121 cggagcaccg gaccgagtta tctgggaaca aagcaggaca agtctgggca cctgaaggat 3181 ctactgcttt caagtgtctg ctttcagcaa ggttatgtgc tgctctcctg agcaacatct 3241 ctgactgtga tgaaacattc aactactggg agccaacaca ctacctcatc tatggggaag 3301 ggtttcagac ttgggaatat tccccagcat atgccattcg ctcctatgct tacctgttgc 3361 ttcatgcctg gccagctgca tttcatgcaa gaattctaca aactaataag attcttgtgt 3421 tttacttttt gcgatgtctt ctggcttttg tgagctgtat ttgtgaactt tacttttaca 3481 aggctgtgtg caagaagttt gggttgcacg tgagtcgaat gatgctagcc ttcttggttc 3541 tcagcactgg catgttttgc tcatcatcag cattccttcc tagtagcttc tgtatgtaca 3601 ctacgttgat agccatgact ggatggtata tggacaagac ttccattgct gtgctgggag 3661 tagcagctgg ggctatctta ggctggccat tcagtgcagc tcttggttta cccattgcct 3721 ttgatttgct ggtcatgaaa cacaggtgga agagtttctt tcattggtcg ctgatggccc 3781 tcatactatt tctggtgcct gtggtggtca ttgacagcta ctattatggg aagttggtga 3841 ttgcaccact caacattgtt ttgtataatg tctttactcc tcatggacct gatctttatg 3901 gtacagaacc ctggtatttc tatttaatta atggatttct gaatttcaat gtagcctttg 3961 ctttggctct cctagtccta ccactgactt ctcttatgga atacctgctg cagagatttc 4021 atgttcagaa tttaggccac ccgtattggc ttaccttggc tccaatgtat atttggttta 4081 taattttctt catccagcct cacaaagagg agagatttct tttccctgtg tatccactta 4141 tatgtctctg tggcgctgtg gctctctctg cacttcagca cagttttctg tacttccaga 4201 aatgttacca ctttgtgttt caacgatatc gcctggagca ctatactgtg acatcgaatt 4261 ggctggcatt aggaactgtc ttcctgtttg ggctcttgtc attttctcgc tctgtggcac 4321 tgttcagagg atatcacggg ccccttgatt tgtatccaga attttaccga attgctacag 4381 acccaaccat ccacactgtc ccagaaggca gacctgtgaa tgtctgtgtg ggaaaagagt 4441 ggtatcgatt tcccagcagc ttccttcttc ctgacaattg gcagcttcag ttcattccat 4501 cagagttcag aggtcagtta ccaaaacctt ttgcagaagg acctctggcc acccggattg 4561 ttcctactga catgaatgac cagaatctag aagagccatc cagatatatt gatatcagta 4621 aatgccatta tttagtggat ttggacacca tgagagaaac accccgggag ccaaaatatt 4681 catccaataa agaagaatgg atcagcttgg cctatagacc attccttgat gcttctagat 4741 cttcaaagct gctgcgggca ttctatgtcc ccttcctgtc agatcagtat acagtgtacg 4801 taaactacac catcctcaaa ccccggaaag caaagcaaat caggaagaaa agtggaggtg 4861 aaaacttgta ctttcaaggc tCCAGCTTTC TTGTACAAAG TGGTATGAGC AAGGGCGAGG 4921 AACTGTTCAC CGGCGTGGTG CCCATCCTGG TGGAACTGGA TGGCGACGTG AACGGCCACA 4981 AGTTCTCTGT GCGGGGAGAG GGCGAAGGCG ACGCCACAAA TGGCAAGCTG ACCCTGAAGT 5041 TCATCTGCAC CACCGGCAAG CTGCCCGTGC CTTGGCCTAC CCTCGTGACC ACACTGACCT 5101 ACGGCGTGCA GTGCTTCAGC AGATACCCCG ACCATATGAA GCGGCACGAC TTCTTCAAGA 5161 GCGCCATGCC CGAGGGCTAC GTGCAGGAAC GGACCATCAG CTTCAAGGAC GACGGCACCT 5221 ACAAGACCAG AGCCGAAGTG AAGTTCGAGG GCGACACCCT CGTGAACCGG ATCGAGCTGA 5281 AGGGCATCGA TTTCAAAGAG GACGGCAACA TCCTGGGCCA CAAGCTGGAG TACAACTTCA 5341 ACAGCCACAA CGTGTACATC ACCGCCGACA AGCAGAAGAA CGGCATCAAG GCCAACTTCA 5401 AGATCCGGCA CAACGTGGAA GATGGCAGCG TGCAGCTGGC CGACCACTAC CAGCAGAACA 5461 CCCCCATCGG AGATGGCCCC GTGCTGCTGC CCGACAACCA CTACCTGAGC ACCCAGAGCG 5521 TGCTGAGCAA GGACCCCAAC GAGAAGCGGG ACCACATGGT GCTGCTGGAA TTCGTGACCG 5581 CCGCTGGCAT CACCCACGGC ATGAGCGGCC TGAACGACAT CTTCGAGGCC CAGAAAATCG 5641 AGTGGCACGA GCACCACCAT CATCACCATC ACCACTGATG ATTCGAAAAA TAATAAGGAT 5701 CCAATCAACC TCTGGATTAC AAAATTTGTG AAAGATTGAC TGGTATTCTT AACTATGTTG 5761 CTCCTTTTAC GCTATGTGGA TACGCTGCTT TAATGCCTTT GTATCATGCT ATTGCTTCCC 5821 GTATGGCTTT CATTTTCTCC TCCTTGTATA AATCCTGGTT GCTGTCTCTT TATGAGGAGT 5881 TGTGGCCCGT TGTCAGGCAA CGTGGCGTGG TGTGCACTGT GTTTGCTGAC GCAACCCCCA 5941 CTGGTTGGGG CATTGCCACC ACCTGTCAGC TCCTTTCCGG GACTTTCGCT TTCCCCCTCC 6001 CTATTGCCAC GGCGGAACTC ATCGCCGCCT GCCTTGCCCG CTGCTGGACA GGGGCTCGGC 6061 TGTTGGGCAC TGACAATTCC GTGGTGTTGT CGGGGAAGCT GACGTCCTTT CCATGGCTGC 6121 TCGCCTGTGT TGCCACCTGG ATTCTGCGCG GGACGTCCTT CTGCTACGTC CCTTCGGCCC 6181 TCAATCCAGC GGACCTTCCT TCCCGCGGCC TGCTGCCGGC TCTGCGGCCT CTTCCGCGTC 6241 TTCGAGATCT GCCTCGACTG TGCCTTCTAG TTGCCAGCCA TCTGTTGTTT GCCCCTCCCC 6301 CGTGCCTTCC TTGACCCTGG AAGGTGCCAC TCCCACTGTC CTTTCCTAAT AAAATGAGGA 6361 AATTGCATCG CATTGTCTGA GTAGGTGTCA TTCTATTCTG GGGGGTGGGG TGGGGCAGGA 6421 CAGCAAGGGG GAGGATTGGG AAGACAATAG CAGGCATGCT GGGGACTCGA GTTAAGGGCG 6481 AATTGCCGAT TAGGATCTTC CTAGAGCATG GCTACGTAGA TAAGTAGCAT GGCGGGTTAA 6541 TCATTAACTA CAAGGAACCC CTAGTGATGG AGTTGGCCAC TCCCTCTCTG CGCGCTCGCT 6601 CGCTCACTGA GGCCGGGCGA CCAAAGGTCG CCCGACGCCC GGGCTTTGCC CGGGCGGCCT 6661 CAGTGAGCGA GCGAGCGCGC AGCCTTAATT AAGAGCTCAT GGCGCGCCTA GGCCTTGACG 6721 GCCTTCCGCC AATTCGCCCT ATAGTGAGTC GTATTACGTC GCGCTCACTG GCCGTCGTTT 6781 TACAACGTCG TGACTGGGAA AACCCTGGCG TTACCCAACT TAATCGCCTT GCAGCACATC 6841 CCCCTTTCGC CAGCTGGCGT AATAGCGAAG AGGCCCGCAC CGAAACGCCC TTCCCAACAG 6901 TTGCGCAGCC TGAATGGCGA ATGGGAGCGC CCTGTAGCGG CCACTCAACC CTATCTCGGT 6961 CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT 7021 GATTTAACAA AAATTTAACG CGAATTTTAA CAAAATATTA ACGCTTACAA TTTAG //