LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2357..2453) /label=attL2 /note="attL2 (c)" misc_signal complement(2468..2487) /note="T7 Promoter/priming site (c)" primer_bind 2495..2511 /note="M13 Reverse priming site" gene 2624..3433 /note="Kanamycin resistance gene" rep_origin 3554..4227 /note="pUC origin" PCR_frame 669..2357 /note="Reading Frame" /set="Set 1" PCR_primer 652..668 /primer="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original=ACAAGTTTGTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC PCR_primer complement(669..2377) /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original=ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG PCR_product complement(652..2374) /pair="Pair 1" /set="Set 1" /current=1 /score=1.000 vector join(2365..4230,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 2335..2355 /note="TEV site" source 673..2334 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:21236 IMAGE:4430974" /tissue_type="Prostate, adenocarcinoma." /clone_lib="NIH_MGC_91" /lab_host="DH10B" /note="Vector: pCMV-SPORT6" gene 673..2334 /gene="PIGB" /gene_synonym=MGC21236 /db_xref="GeneID:9488" /db_xref="HGNC:8959" /db_xref="MIM:604122" CDS 673..2334 /dnas_title="phosphatidylinositol glycan anchor biosynthesis, class B" /gene="PIGB" /gene_synonym=MGC21236 /codon_start=1 /product="phosphatidylinositol glycan anchor biosynthesis, class B" /protein_id="AAH17711.1" /db_xref="GI:17389331" /db_xref="GeneID:9488" /db_xref="HGNC:8959" /db_xref="MIM:604122" /translation="MRRPLSKCGMEPGGGDASLTLHGLQNRSHGKIKLRKRKSTLYFN TQEKSARRRGDLLGENIYLLLFTLALRILNCFLVQTSFVPDEYWQSLEVSHHMVFNYG YLTWEWTERLRSYTYPLIFASIYKILHLLGKDSVQLLIWIPRLAQALLSAVADVRLYS LMKQLENQEVARWVFFCQLCSWFTWYCCTRTLTNTMETVLTIIALFYYPLEGSKSMNS VKYSSLVALAFIIRPTAVILWTPLLFRHFCQEPRKLDLILHHFLPVGFVTLSLSLMID RIFFGQWTLVQFNFLKFNVLQNLGTFYGSHPWHWYFSQGFPVILGTHLPFFIHGCYLA PKRYRILLVTVLWTLLVYSMLSHKEFRFIYPVLPFCMVFCGYSLTHLKTWKKPALSFL FLSNLFLALYTGLVHQRGTLDVMSHIQKVCYNNPNKSSASIFIMMPCHSTPYYSHVHC PLPMRFLQCPPDLTGKSHYLDEADVFYLNPLNWSHREFHDDASLPTHLITFSILEEEI SAFLISSNYKRTAVFFHTHLPEGRIGSHIYVYERKLKGKFNMKMKF" misc_difference 874..874 /gene="PIGB" /gene_synonym=MGC21236 /note="'T' in cDNA is 'A' in the human genome; amino acid difference: 'L' in cDNA, 'I' in the human genome. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." misc_feature 673..2334 /note="PIGB coding region" misc_difference 1568..1568 /gene="PIGB" /gene_synonym=MGC21236 /note="'T' in cDNA is 'G' in the human genome; amino acid difference: 'L' in cDNA, 'W' in the human genome." misc_difference 2123..2123 /gene="PIGB" /gene_synonym=MGC21236 /note="'C' in cDNA is 'T' in the human genome; amino acid difference: 'S' in cDNA, 'L' in the human genome. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." misc_difference 2199..2199 /gene="PIGB" /gene_synonym=MGC21236 /note="'T' in cDNA is 'C' in the human genome; no amino acid change. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTCG CCatgaggag gcccctaagc aagtgcggaa tggagccggg gggcggagat 721 gccagcctca ctttgcatgg tctccagaac cgctcccacg gcaagataaa gctgcgaaag 781 agaaagtcta ccttgtactt caacacccag gagaagagcg ccaggcgccg cggggatctt 841 cttggagaaa atatttatct gctcttgttt accttagctt tacgaatatt aaactgcttt 901 ttagtgcaga caagttttgt tccagatgaa tactggcagt ctcttgaagt ttcacatcac 961 atggttttca attatggtta tttgacttgg gaatggacag agagactgag gagttacact 1021 tatcccttaa tctttgcaag catttacaag attcttcatc ttttagggaa agatagtgtt 1081 cagttgctga tttggattcc tagacttgcc caagcacttc tgtctgctgt agcagatgtg 1141 agactttact cattaatgaa gcaactagaa aatcaggaag tggcaagatg ggtgtttttt 1201 tgccagttgt gctcctggtt cacatggtat tgctgtacca gaacccttac aaacaccatg 1261 gaaactgttc tcactataat tgctcttttc tactatcctt tggaaggttc aaagtctatg 1321 aacagtgtca aatactcatc cctggtggca cttgccttca taattcgtcc cacagctgtc 1381 attctgtgga cacctttgct cttcagacat ttctgtcaag aaccaagaaa gcttgatctt 1441 attctacatc attttttacc tgtaggcttt gttactttga gtttgtctct gatgattgat 1501 cgtatttttt ttggccaatg gactctggtt caatttaatt ttttgaaatt taacgtgctg 1561 cagaacttgg gaacatttta tggttctcat ccatggcact ggtacttcag tcaaggattt 1621 ccagttatct tgggtactca cttacccttc tttattcatg gctgctatct agcaccaaag 1681 agataccgga tacttttggt gactgtgctg tggacactgc ttgtttatag catgttgagc 1741 cacaaagaat tcaggtttat ttatccagtt ttaccattct gtatggtgtt ctgtggatac 1801 tcattaaccc acctgaaaac atggaagaaa ccagctctaa gtttcctgtt tttatcaaat 1861 ttgttcctcg ccctttatac tggtttagtt catcaacgag gtactcttga tgtcatgagt 1921 catattcaaa aagtttgtta caacaatccc aataaatctt cagcttcaat atttataatg 1981 atgccttgcc actctactcc ttattacagc catgttcact gcccacttcc catgagattt 2041 ctccagtgcc cgccagacct gactggaaaa agtcattatc ttgatgaagc agatgtattt 2101 tacctaaatc ccttaaactg gtcacataga gagtttcatg atgatgcatc attgcctact 2161 cacttgatca ccttcagcat tttggaagag gaaataagtg ctttcctaat ttcaagcaat 2221 tataaaagaa ctgctgtttt cttccacact cacttgccag agggtcgaat tggaagtcac 2281 atatatgtct atgaacggaa gttaaaaggg aaattcaaca tgaagatgaa attcgaaaac 2341 ttgtactttc aaggctCCAG CTTTCTTGTA CAAAGTTGGC ATTATAAGAA AGCATTGCTT 2401 ATCAATTTGT TGCAACGAAC AGGTCACTAT CAGTCAAAAT AAAATCATTA TTTGCCATCC 2461 AGCTGATATC CCCTATAGTG AGTCGTATTA CATGGTCATA GCTGTTTCCT GGCAGCTCTG 2521 GCCCGTGTCT CAAAATCTCT GATGTTACAT TGCACAAGAT AAAATAATAT CATCATGAAC 2581 AATAAAACTG TCTGCTTACA TAAACAGTAA TACAAGGGGT GTTATGAGCC ATATTCAACG 2641 GGAAACGTCG AGGCCGCGAT TAAATTCCAA CATGGATGCT GATTTATATG GGTATAAATG 2701 GGCTCGCGAT AATGTCGGGC AATCAGGTGC GACAATCTAT CGCTTGTATG GGAAGCCCGA 2761 TGCGCCAGAG TTGTTTCTGA AACATGGCAA AGGTAGCGTT GCCAATGATG TTACAGATGA 2821 GATGGTCAGA CTAAACTGGC TGACGGAATT TATGCCTCTT CCGACCATCA AGCATTTTAT 2881 CCGTACTCCT GATGATGCAT GGTTACTCAC CACTGCGATC CCCGGAAAAA CAGCATTCCA 2941 GGTATTAGAA GAATATCCTG ATTCAGGTGA AAATATTGTT GATGCGCTGG CAGTGTTCCT 3001 GCGCCGGTTG CATTCGATTC CTGTTTGTAA TTGTCCTTTT AACAGCGATC GCGTATTTCG 3061 TCTCGCTCAG GCGCAATCAC GAATGAATAA CGGTTTGGTT GATGCGAGTG ATTTTGATGA 3121 CGAGCGTAAT GGCTGGCCTG TTGAACAAGT CTGGAAAGAA ATGCATAAAC TTTTGCCATT 3181 CTCACCGGAT TCAGTCGTCA CTCATGGTGA TTTCTCACTT GATAACCTTA TTTTTGACGA 3241 GGGGAAATTA ATAGGTTGTA TTGATGTTGG ACGAGTCGGA ATCGCAGACC GATACCAGGA 3301 TCTTGCCATC CTATGGAACT GCCTCGGTGA GTTTTCTCCT TCATTACAGA AACGGCTTTT 3361 TCAAAAATAT GGTATTGATA ATCCTGATAT GAATAAATTG CAGTTTCATT TGATGCTCGA 3421 TGAGTTTTTC TAATCAGAAT TGGTTAATTG GTTGTAACAC TGGCAGAGCA TTACGCTGAC 3481 TTGACGGGAC GGCGCAAGCT CATGACCAAA ATCCCTTAAC GTGAGTTACG CGTCGTTCCA 3541 CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG 3601 CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA 3661 TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA 3721 TACTGTTCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC 3781 TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG 3841 TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC 3901 GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT 3961 ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC 4021 GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG 4081 GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG 4141 CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT 4201 GGCCTTTTGC TGGCCTTTTG CTCACATGTT //