LOCUS PIGB-pGEc2-DEST 6883 bp DNA circular 22-JAN-2013 FEATURES Location/Qualifiers misc_feature complement(6361..6490) /note="3'-ITR" misc_feature complement(6059..6273) /note="BGH pA" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" CDS 129..989 /dnas_title="AmpR" /label=AmpR /vntifkey="4" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_feature 5511..6052 /note="WPRE" misc_recomb complement(2974..3001) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_feature 4690..4712 /note="attB2" misc_feature 5490..5495 /note="BstB1 site" misc_feature 4713..5408 /note="Super GFP" misc_feature 5409..5459 /note="AviTag" misc_feature 5460..5483 /note="His Tag" PCR_frame 3002..4690 /note="Reading Frame" /set="Set 1" source 3006..4667 /dnas_title="PIGB-pGEc2-DEST" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:21236 IMAGE:4430974" /tissue_type="Prostate, adenocarcinoma." /clone_lib="NIH_MGC_91" /lab_host="DH10B" /note="Vector: pCMV-SPORT6" gene 3006..4667 /dnas_title="PIGB" /gene="PIGB" /gene_synonym="MGC21236" /db_xref="GeneID:9488" /db_xref="HGNC:8959" /db_xref="MIM:604122" CDS 3006..4667 /dnas_title="phosphatidylinositol glycan anchor biosynthesis, class B" /gene="PIGB" /gene_synonym="MGC21236" /codon_start=1 /product="phosphatidylinositol glycan anchor biosynthesis, class B" /protein_id="AAH17711.1" /db_xref="GI:17389331" /db_xref="GeneID:9488" /db_xref="HGNC:8959" /db_xref="MIM:604122" /translation="MRRPLSKCGMEPGGGDASLTLHGLQNRSHGKIKLRKRKSTLYFN TQEKSARRRGDLLGENIYLLLFTLALRILNCFLVQTSFVPDEYWQSLEVSHHMVFNYG YLTWEWTERLRSYTYPLIFASIYKILHLLGKDSVQLLIWIPRLAQALLSAVADVRLYS LMKQLENQEVARWVFFCQLCSWFTWYCCTRTLTNTMETVLTIIALFYYPLEGSKSMNS VKYSSLVALAFIIRPTAVILWTPLLFRHFCQEPRKLDLILHHFLPVGFVTLSLSLMID RIFFGQWTLVQFNFLKFNVLQNLGTFYGSHPWHWYFSQGFPVILGTHLPFFIHGCYLA PKRYRILLVTVLWTLLVYSMLSHKEFRFIYPVLPFCMVFCGYSLTHLKTWKKPALSFL FLSNLFLALYTGLVHQRGTLDVMSHIQKVCYNNPNKSSASIFIMMPCHSTPYYSHVHC PLPMRFLQCPPDLTGKSHYLDEADVFYLNPLNWSHREFHDDASLPTHLITFSILEEEI SAFLISSNYKRTAVFFHTHLPEGRIGSHIYVYERKLKGKFNMKMKF" misc_difference 3207..3207 /dnas_title="PIGB" /gene="PIGB" /gene_synonym="MGC21236" /note="'T' in cDNA is 'A' in the human genome; amino acid difference: 'L' in cDNA, 'I' in the human genome. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." misc_feature 3006..4667 /note="PIGB coding region" misc_difference 3901..3901 /dnas_title="PIGB" /gene="PIGB" /gene_synonym="MGC21236" /note="'T' in cDNA is 'G' in the human genome; amino acid difference: 'L' in cDNA, 'W' in the human genome." misc_feature 4668..4688 /note="TEV site" misc_difference 4456..4456 /dnas_title="PIGB" /gene="PIGB" /gene_synonym="MGC21236" /note="'C' in cDNA is 'T' in the human genome; amino acid difference: 'S' in cDNA, 'L' in the human genome. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." misc_difference 4532..4532 /dnas_title="PIGB" /gene="PIGB" /gene_synonym="MGC21236" /note="'T' in cDNA is 'C' in the human genome; no amino acid change. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." PCR_primer 2992..3001 /dnas_title="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /primer="CAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" PCR_primer complement(3002..4697) /dnas_title="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original="ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG" PCR_product complement(2992..4697) /pair="Pair 1" /set="Set 1" /current=1 /score="1.000" vector join(4698..6883,1..2991) /source="pGEc2-DEST" /type="Destination Vector" clone 2992..4697 /source="PIGB-C-term-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCATCACAA GTTTGTACAA AAAAGCAGGC 3001 TCGCCatgag gaggccccta agcaagtgcg gaatggagcc ggggggcgga gatgccagcc 3061 tcactttgca tggtctccag aaccgctccc acggcaagat aaagctgcga aagagaaagt 3121 ctaccttgta cttcaacacc caggagaaga gcgccaggcg ccgcggggat cttcttggag 3181 aaaatattta tctgctcttg tttaccttag ctttacgaat attaaactgc tttttagtgc 3241 agacaagttt tgttccagat gaatactggc agtctcttga agtttcacat cacatggttt 3301 tcaattatgg ttatttgact tgggaatgga cagagagact gaggagttac acttatccct 3361 taatctttgc aagcatttac aagattcttc atcttttagg gaaagatagt gttcagttgc 3421 tgatttggat tcctagactt gcccaagcac ttctgtctgc tgtagcagat gtgagacttt 3481 actcattaat gaagcaacta gaaaatcagg aagtggcaag atgggtgttt ttttgccagt 3541 tgtgctcctg gttcacatgg tattgctgta ccagaaccct tacaaacacc atggaaactg 3601 ttctcactat aattgctctt ttctactatc ctttggaagg ttcaaagtct atgaacagtg 3661 tcaaatactc atccctggtg gcacttgcct tcataattcg tcccacagct gtcattctgt 3721 ggacaccttt gctcttcaga catttctgtc aagaaccaag aaagcttgat cttattctac 3781 atcatttttt acctgtaggc tttgttactt tgagtttgtc tctgatgatt gatcgtattt 3841 tttttggcca atggactctg gttcaattta attttttgaa atttaacgtg ctgcagaact 3901 tgggaacatt ttatggttct catccatggc actggtactt cagtcaagga tttccagtta 3961 tcttgggtac tcacttaccc ttctttattc atggctgcta tctagcacca aagagatacc 4021 ggatactttt ggtgactgtg ctgtggacac tgcttgttta tagcatgttg agccacaaag 4081 aattcaggtt tatttatcca gttttaccat tctgtatggt gttctgtgga tactcattaa 4141 cccacctgaa aacatggaag aaaccagctc taagtttcct gtttttatca aatttgttcc 4201 tcgcccttta tactggttta gttcatcaac gaggtactct tgatgtcatg agtcatattc 4261 aaaaagtttg ttacaacaat cccaataaat cttcagcttc aatatttata atgatgcctt 4321 gccactctac tccttattac agccatgttc actgcccact tcccatgaga tttctccagt 4381 gcccgccaga cctgactgga aaaagtcatt atcttgatga agcagatgta ttttacctaa 4441 atcccttaaa ctggtcacat agagagtttc atgatgatgc atcattgcct actcacttga 4501 tcaccttcag cattttggaa gaggaaataa gtgctttcct aatttcaagc aattataaaa 4561 gaactgctgt tttcttccac actcacttgc cagagggtcg aattggaagt cacatatatg 4621 tctatgaacg gaagttaaaa gggaaattca acatgaagat gaaattcgaa aacttgtact 4681 ttcaaggctC CAGCTTTCTT GTACAAAGTG GTATGAGCAA GGGCGAGGAA CTGTTCACCG 4741 GCGTGGTGCC CATCCTGGTG GAACTGGATG GCGACGTGAA CGGCCACAAG TTCTCTGTGC 4801 GGGGAGAGGG CGAAGGCGAC GCCACAAATG GCAAGCTGAC CCTGAAGTTC ATCTGCACCA 4861 CCGGCAAGCT GCCCGTGCCT TGGCCTACCC TCGTGACCAC ACTGACCTAC GGCGTGCAGT 4921 GCTTCAGCAG ATACCCCGAC CATATGAAGC GGCACGACTT CTTCAAGAGC GCCATGCCCG 4981 AGGGCTACGT GCAGGAACGG ACCATCAGCT TCAAGGACGA CGGCACCTAC AAGACCAGAG 5041 CCGAAGTGAA GTTCGAGGGC GACACCCTCG TGAACCGGAT CGAGCTGAAG GGCATCGATT 5101 TCAAAGAGGA CGGCAACATC CTGGGCCACA AGCTGGAGTA CAACTTCAAC AGCCACAACG 5161 TGTACATCAC CGCCGACAAG CAGAAGAACG GCATCAAGGC CAACTTCAAG ATCCGGCACA 5221 ACGTGGAAGA TGGCAGCGTG CAGCTGGCCG ACCACTACCA GCAGAACACC CCCATCGGAG 5281 ATGGCCCCGT GCTGCTGCCC GACAACCACT ACCTGAGCAC CCAGAGCGTG CTGAGCAAGG 5341 ACCCCAACGA GAAGCGGGAC CACATGGTGC TGCTGGAATT CGTGACCGCC GCTGGCATCA 5401 CCCACGGCAT GAGCGGCCTG AACGACATCT TCGAGGCCCA GAAAATCGAG TGGCACGAGC 5461 ACCACCATCA TCACCATCAC CACTGATGAT TCGAAAAATA ATAAGGATCC AATCAACCTC 5521 TGGATTACAA AATTTGTGAA AGATTGACTG GTATTCTTAA CTATGTTGCT CCTTTTACGC 5581 TATGTGGATA CGCTGCTTTA ATGCCTTTGT ATCATGCTAT TGCTTCCCGT ATGGCTTTCA 5641 TTTTCTCCTC CTTGTATAAA TCCTGGTTGC TGTCTCTTTA TGAGGAGTTG TGGCCCGTTG 5701 TCAGGCAACG TGGCGTGGTG TGCACTGTGT TTGCTGACGC AACCCCCACT GGTTGGGGCA 5761 TTGCCACCAC CTGTCAGCTC CTTTCCGGGA CTTTCGCTTT CCCCCTCCCT ATTGCCACGG 5821 CGGAACTCAT CGCCGCCTGC CTTGCCCGCT GCTGGACAGG GGCTCGGCTG TTGGGCACTG 5881 ACAATTCCGT GGTGTTGTCG GGGAAGCTGA CGTCCTTTCC ATGGCTGCTC GCCTGTGTTG 5941 CCACCTGGAT TCTGCGCGGG ACGTCCTTCT GCTACGTCCC TTCGGCCCTC AATCCAGCGG 6001 ACCTTCCTTC CCGCGGCCTG CTGCCGGCTC TGCGGCCTCT TCCGCGTCTT CGAGATCTGC 6061 CTCGACTGTG CCTTCTAGTT GCCAGCCATC TGTTGTTTGC CCCTCCCCCG TGCCTTCCTT 6121 GACCCTGGAA GGTGCCACTC CCACTGTCCT TTCCTAATAA AATGAGGAAA TTGCATCGCA 6181 TTGTCTGAGT AGGTGTCATT CTATTCTGGG GGGTGGGGTG GGGCAGGACA GCAAGGGGGA 6241 GGATTGGGAA GACAATAGCA GGCATGCTGG GGACTCGAGT TAAGGGCGAA TTGCCGATTA 6301 GGATCTTCCT AGAGCATGGC TACGTAGATA AGTAGCATGG CGGGTTAATC ATTAACTACA 6361 AGGAACCCCT AGTGATGGAG TTGGCCACTC CCTCTCTGCG CGCTCGCTCG CTCACTGAGG 6421 CCGGGCGACC AAAGGTCGCC CGACGCCCGG GCTTTGCCCG GGCGGCCTCA GTGAGCGAGC 6481 GAGCGCGCAG CCTTAATTAA GAGCTCATGG CGCGCCTAGG CCTTGACGGC CTTCCGCCAA 6541 TTCGCCCTAT AGTGAGTCGT ATTACGTCGC GCTCACTGGC CGTCGTTTTA CAACGTCGTG 6601 ACTGGGAAAA CCCTGGCGTT ACCCAACTTA ATCGCCTTGC AGCACATCCC CCTTTCGCCA 6661 GCTGGCGTAA TAGCGAAGAG GCCCGCACCG AAACGCCCTT CCCAACAGTT GCGCAGCCTG 6721 AATGGCGAAT GGGAGCGCCC TGTAGCGGCC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA 6781 TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA 6841 ATTTAACGCG AATTTTAACA AAATATTAAC GCTTACAATT TAG //