LOCUS PIGZ-pGEc1-DEST 6232 bp DNA circular 22-JAN-2013 FEATURES Location/Qualifiers misc_feature complement(5710..5839) /note="3'-ITR" misc_feature complement(5408..5622) /note="BGH pA" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" CDS 129..989 /dnas_title="AmpR" /label=AmpR /vntifkey="4" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_feature 4860..5401 /note="WPRE" misc_recomb complement(2974..3001) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_recomb 4765..4787 /label=attB2 /note="attB2 site" misc_feature 4797..4820 /note="8xHis Tag" misc_feature 4824..4847 /note="StrepTag" PCR_frame 3002..4765 /note="Reading Frame" /set="Set 1" source 3006..4742 /dnas_title="PIGZ-pGEc1-DEST" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:52163 IMAGE:4871581" /tissue_type="Skin, melanotic melanoma, high MDR." /clone_lib="NIH_MGC_49" /lab_host="DH10B-R" /note="Vector: pOTB7" gene 3006..4742 /dnas_title="PIGZ" /gene="PIGZ" /gene_synonym="FLJ12768" /gene_synonym="MGC52163" /gene_synonym="SMP3" /db_xref="GeneID:80235" /db_xref="HGNC:30596" CDS 3006..4742 /dnas_title="phosphatidylinositol glycan anchor biosynthesis, class Z" /gene="PIGZ" /gene_synonym="FLJ12768" /gene_synonym="MGC52163" /gene_synonym="SMP3" /codon_start=1 /product="phosphatidylinositol glycan anchor biosynthesis, class Z" /protein_id="AAH44640.1" /db_xref="GI:27881689" /db_xref="GeneID:80235" /db_xref="HGNC:30596" /translation="MQICGSSVASVAAGTSFQVLGPVCWQQLDLKMAVRVLWGGLSLL RVLWCLLPQTGYVHPDEFFQSPEVMAEDILGVQAARPWEFYPSSSCRSVLFPLLISGS TFWLLRLWEELGPWPGLVSGYALLVGPRLLLTALSFALDGAVYHLAPPMGADRWNALA LLSGSYVTLVFYTRTFSNTIEGLLFTWLLVLVSSHVTWGPTRKEPAPGPRWRSWLLGG IVAAGFFNRPTFLAFAVVPLYLWGTRGATNPGLKSLTREALVLLPGATLTAAVFVATD SWYFSSPATSRNLVLTPVNFLHYNLNPQNLARHGTHARLTHLAVNGFLLFGVLHAQAL QAAWQQLQVGLQASAQMGLLRALGARSLLSSPRSYLLLLYFMPLALLSAFSHQEARFL IPLLVPLVLLCSPQTQPVPWKGTVVLFNALGALLFGCLHQGGLVPGLEYLEQVVHAPV LPSTPTHYTLLFTHTYMPPRHLLHLPGLGAPVEVVDIGGTEDWALCQTLKSFTRQPAC QVAGGPWLCRLFVVTPGTTRRAVEKCSFPFKNETLLFPHLTLEDPPALSSLLSGAWRD HLSLHIVELGEET" misc_difference 3305..3305 /dnas_title="PIGZ" /gene="PIGZ" /gene_synonym="FLJ12768" /gene_synonym="MGC52163" /gene_synonym="SMP3" /note="'C' in cDNA is 'T' in the human genome; no amino acid change." misc_feature 3006..4742 /note="PIGZ coding region" misc_difference 3800..3801 /dnas_title="PIGZ" /gene="PIGZ" /gene_synonym="FLJ12768" /gene_synonym="MGC52163" /gene_synonym="SMP3" /note="in the human genome : AG." misc_difference 3894..3894 /dnas_title="PIGZ" /gene="PIGZ" /gene_synonym="FLJ12768" /gene_synonym="MGC52163" /gene_synonym="SMP3" /note="'C' in cDNA is 'T' in the human genome; no amino acid change. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." misc_difference 4024..4024 /dnas_title="PIGZ" /gene="PIGZ" /gene_synonym="FLJ12768" /gene_synonym="MGC52163" /gene_synonym="SMP3" /note="'A' in cDNA is 'G' in the human genome; amino acid difference: 'Q' in cDNA, 'R' in the human genome." misc_feature 4743..4763 /note="TEV site" misc_difference 4466..4466 /dnas_title="PIGZ" /gene="PIGZ" /gene_synonym="FLJ12768" /gene_synonym="MGC52163" /gene_synonym="SMP3" /note="'A' in cDNA is 'G' in the human genome; amino acid difference: 'I' in cDNA, 'M' in the human genome. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." PCR_primer 2992..3001 /dnas_title="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /primer="CAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" PCR_primer complement(3002..4772) /dnas_title="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original="ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG" PCR_product complement(2992..4772) /pair="Pair 1" /set="Set 1" /current=1 /score="1.000" vector join(4773..6232,1..2991) /source="pGEc1-DEST" /type="Destination Vector" clone 2992..4772 /source="PIGZ-C-term-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCATCACAA GTTTGTACAA AAAAGCAGGC 3001 TCGCCatgca gatctgtgga tccagcgtag catctgtagc agctgggaca tcattccagg 3061 ttttgggccc ggtgtgttgg caacaactgg atctgaagat ggcagtcagg gtgctttggg 3121 gtggtctcag cctgctccga gtgctgtggt gtctccttcc gcagacgggc tatgtgcacc 3181 cagatgagtt cttccagtcc cctgaggtga tggcagagga catcctgggc gttcaggccg 3241 cgcggccctg ggagttttac cccagcagct cctgccgctc ggtgctcttc cccctgctga 3301 tctccggttc caccttctgg ctgctcaggc tctgggagga gctggggccg tggcctggcc 3361 tggtgagcgg ctatgcgctg ctggtggggc ctcgactcct cctcactgcc ctttcctttg 3421 ctctggacgg ggccgtgtac cacctggccc cgccgatggg ggcggatcgc tggaacgccc 3481 tggccctgct gtctggttcc tacgtcaccc tggtcttcta cacaaggacc ttctccaaca 3541 ccattgaggg actcctcttc acgtggctgc tggtgctggt atcctcccat gtaacgtggg 3601 gccctacacg caaggagccg gcgccgggtc cacggtggcg cagctggctt cttggaggca 3661 ttgtggctgc tggcttcttc aaccggccca cctttctggc ctttgctgtg gtccccctct 3721 acctctgggg cactcgtgga gccacaaacc ctggtttgaa gtctctgacc cgggaggccc 3781 tggtgctgct ccctggggcg accctcacag cagcggtgtt tgtggccacg gacagctggt 3841 atttctccag ccccgctaca tccaggaacc ttgtcctgac acctgtcaac ttcctgcact 3901 acaacctgaa tccccaaaac ctggcgagac atggcacgca cgcgcggctc actcacctgg 3961 cagtcaacgg cttcctgctc ttcggggtgc tgcatgccca ggccctgcag gctgcgtggc 4021 aacagctgca agtcggcctc caggcctctg cacaaatggg cctcctgagg gcactgggtg 4081 cccggagcct gctgtccagc cccaggtcct atctccttct cctctacttc atgcctctgg 4141 ccctgctatc tgcctttagc caccaggagg ctcggttcct gattcccctc ctggtccccc 4201 tggtcctgct ttgtagtcca cagacgcagc ctgtgccttg gaagggcact gtggtcctct 4261 tcaacgccct cggtgccctc ctcttcggct gcctgcatca ggggggcctg gtgcctggcc 4321 tggagtacct ggagcaggtg gtccatgccc ctgtgctccc aagcacaccc acccactaca 4381 cactcctctt cactcacacc tacatgcccc cccggcacct cctacacctc ccaggcctgg 4441 gggcaccagt ggaggtggtg gacatagggg ggactgagga ctgggccctg tgccaaaccc 4501 tgaaaagctt caccagacaa ccagcctgcc aagtggctgg tgggccatgg ctctgccgcc 4561 tctttgtggt aacccctggc accaccaggc gtgccgtgga gaagtgcagc ttccccttca 4621 agaatgaaac acttttattt ccccatctga ccctggagga tccaccagcc ctgtcctcct 4681 tgctgagtgg ggcttggagg gaccacctca gtcttcacat tgtggagctg ggggaagaaa 4741 ccgaaaactt gtactttcaa ggctCCAGCT TTCTTGTACA AAGTGGTGAT GGCCGCCATC 4801 ATCATCATCA TCATCATCAT GCGTGGAGCC ACCCGCAGTT CGAAAAATAA TAAGGATCCA 4861 ATCAACCTCT GGATTACAAA ATTTGTGAAA GATTGACTGG TATTCTTAAC TATGTTGCTC 4921 CTTTTACGCT ATGTGGATAC GCTGCTTTAA TGCCTTTGTA TCATGCTATT GCTTCCCGTA 4981 TGGCTTTCAT TTTCTCCTCC TTGTATAAAT CCTGGTTGCT GTCTCTTTAT GAGGAGTTGT 5041 GGCCCGTTGT CAGGCAACGT GGCGTGGTGT GCACTGTGTT TGCTGACGCA ACCCCCACTG 5101 GTTGGGGCAT TGCCACCACC TGTCAGCTCC TTTCCGGGAC TTTCGCTTTC CCCCTCCCTA 5161 TTGCCACGGC GGAACTCATC GCCGCCTGCC TTGCCCGCTG CTGGACAGGG GCTCGGCTGT 5221 TGGGCACTGA CAATTCCGTG GTGTTGTCGG GGAAGCTGAC GTCCTTTCCA TGGCTGCTCG 5281 CCTGTGTTGC CACCTGGATT CTGCGCGGGA CGTCCTTCTG CTACGTCCCT TCGGCCCTCA 5341 ATCCAGCGGA CCTTCCTTCC CGCGGCCTGC TGCCGGCTCT GCGGCCTCTT CCGCGTCTTC 5401 GAGATCTGCC TCGACTGTGC CTTCTAGTTG CCAGCCATCT GTTGTTTGCC CCTCCCCCGT 5461 GCCTTCCTTG ACCCTGGAAG GTGCCACTCC CACTGTCCTT TCCTAATAAA ATGAGGAAAT 5521 TGCATCGCAT TGTCTGAGTA GGTGTCATTC TATTCTGGGG GGTGGGGTGG GGCAGGACAG 5581 CAAGGGGGAG GATTGGGAAG ACAATAGCAG GCATGCTGGG GACTCGAGTT AAGGGCGAAT 5641 TGCCGATTAG GATCTTCCTA GAGCATGGCT ACGTAGATAA GTAGCATGGC GGGTTAATCA 5701 TTAACTACAA GGAACCCCTA GTGATGGAGT TGGCCACTCC CTCTCTGCGC GCTCGCTCGC 5761 TCACTGAGGC CGGGCGACCA AAGGTCGCCC GACGCCCGGG CTTTGCCCGG GCGGCCTCAG 5821 TGAGCGAGCG AGCGCGCAGC CTTAATTAAG AGCTCATGGC GCGCCTAGGC CTTGACGGCC 5881 TTCCGCCAAT TCGCCCTATA GTGAGTCGTA TTACGTCGCG CTCACTGGCC GTCGTTTTAC 5941 AACGTCGTGA CTGGGAAAAC CCTGGCGTTA CCCAACTTAA TCGCCTTGCA GCACATCCCC 6001 CTTTCGCCAG CTGGCGTAAT AGCGAAGAGG CCCGCACCGA AACGCCCTTC CCAACAGTTG 6061 CGCAGCCTGA ATGGCGAATG GGAGCGCCCT GTAGCGGCCA CTCAACCCTA TCTCGGTCTA 6121 TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT 6181 TTAACAAAAA TTTAACGCGA ATTTTAACAA AATATTAACG CTTACAATTT AG //