LOCUS FUT8-pGEn1-DEST 6187 bp DNA circular 22-JAN-2013 FEATURES Location/Qualifiers CDS 129..1108 /dnas_title="AmpR" /label=AmpR /vntifkey="4" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_feature 2977..3054 /note="TCM Sig Seq" misc_recomb complement(3114..3142) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_feature 3055..3078 /note="8xHis Tag" misc_feature 3085..3108 /note="StrepTag" misc_feature 4815..5356 /note="WPRE" misc_recomb 4777..4799 /label=attB2 /note="attB2 site" /note="recognized as attB2" misc_feature complement(5363..5577) /note="BGH pA" misc_feature complement(5665..5794) /note="3'-ITR" misc_feature 3143..3165 /note="TEV site" source 3166..4770 /dnas_title="FUT8-pGEn1-DEST" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" CDS 3166..4770 /dnas_title="N-Acetyl-beta-D-glucosaminide" /note="GDP-L-Fuc; alpha 1-6 Fucosyltransferase; alpha1-6 FucT" /codon_start=1 /product="N-Acetyl-beta-D-glucosaminide" /protein_id="BAA19764.1" /db_xref="GI:2055307" /translation="MRPWTGSWRWIMLILFAWGTLLFYIGGHLVRDNDHPDHSSRELS KILAKLERLKQQNEDLRRMAESLRIPEGPIDQGPAIGRVRVLEEQLVKAKEQIENYKK QTRNGLGKDHEILRRRIENGAKELWFFLQSELKKLKNLEGNELQRHADEFLLDLGHHE RSIMTDLYYLSQTDGAGDWREKEAKDLTELVQRRITYLQNPKDCSKAKKLVCNINKGC GYGCQLHHVVYCFMIAYGTQRTLILESQNWRYATGGWETVFRPVSETCTDRSGISTGH WSGEVKDKNVQVVELPIVDSLHPRPPYLPLAVPEDLADRLVRVHGDPAVWWVSQFVKY LIRPQPWLEKEIEEATKKLGFKHPVIGVHVRRTDKVGTEAAFHPIEEYMVHVEEHFQL LARRMQVDKKRVYLATDDPSLLKEAKTKYPNYEFISDNSISWSAGLHNRYTENSLRGV ILDIHFLSQADFLVCTFSSQVCRVAYEIMQTLHPDASANFHSLDDIYYFGGQNAHNQI AIYAHQPRTADEIPMEPGDIIGVAGNHWDGYSKGVNRKLGRTGLYPSYKVREKIETVK YPTYPEAEK" misc_feature 3166..4770 /note="FUT8 coding region" vector join(4785..6187,1..3132) /source="pGEn1-DEST" /type="Destination Vector" clone 3133..4784 /source="FUT8-Nterm-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCGCCATGA GACTGCTGAC AGCCCTGTTC 3001 GCCTACTTCA TCGTGGCCCT GATCCTGGCC TTCAGCGTGT CCGCCAAGAG CATGCACCAC 3061 CACCATCACC ATCATCACAG CGCCTGGTCC CACCCCCAGT TCGAGAAAGA ATTATCAACA 3121 AGTTTGTACA AAAAAGCAGG CTctgaaaac ttgtactttc aaggccgaga actgtccaag 3181 attctggcaa agcttgaacg cttaaaacag cagaatgaag acttgaggcg aatggccgaa 3241 tctctccgga taccagaagg ccctattgat caggggccag ctataggaag agtacgcgtt 3301 ttagaagagc agcttgttaa ggccaaagaa cagattgaaa attacaagaa acagaccaga 3361 aatggtctgg ggaaggatca tgaaatcctg aggaggagga ttgaaaatgg agctaaagag 3421 ctctggtttt tcctacagag tgaattgaag aaattaaaga acttagaagg aaatgaactc 3481 caaagacatg cagatgaatt tcttttggat ttaggacatc atgaaaggtc tataatgacg 3541 gatctatact acctcagtca gacagatgga gcaggtgatt ggcgggaaaa agaggccaaa 3601 gatctgacag aactggttca gcggagaata acatatcttc agaatcccaa ggactgcagc 3661 aaagccaaaa agctggtgtg taatatcaac aaaggctgtg gctatggctg tcagctccat 3721 catgtggtct actgcttcat gattgcatat ggcacccagc gaacactcat cttggaatct 3781 cagaattggc gctatgctac tggtggatgg gagactgtat ttaggcctgt aagtgagaca 3841 tgcacagaca gatctggcat ctccactgga cactggtcag gtgaagtgaa ggacaaaaat 3901 gttcaagtgg tcgagcttcc cattgtagac agtcttcatc cccgtcctcc atatttaccc 3961 ttggctgtac cagaagacct cgcagatcga cttgtacgag tgcatggtga ccctgcagtg 4021 tggtgggtgt ctcagtttgt caaatacttg atccgcccac agccttggct agaaaaagaa 4081 atagaagaag ccaccaagaa gcttggcttc aaacatccag ttattggagt ccatgtcaga 4141 cgcacagaca aagtgggaac agaagctgcc ttccatccca ttgaagagta catggtgcat 4201 gttgaagaac attttcagct tcttgcacgc agaatgcaag tggacaaaaa aagagtgtat 4261 ttggccacag atgacccttc tttattaaag gaggcaaaaa caaagtaccc caattatgaa 4321 tttattagtg ataactctat ttcctggtca gctggactgc acaatcgata cacagaaaat 4381 tcacttcgtg gagtgatcct ggatatacat tttctctctc aggcagactt cctagtgtgt 4441 actttttcat cccaggtctg tcgagttgct tatgaaatta tgcaaacact acatcctgat 4501 gcctctgcaa acttccattc tttagatgac atctactatt ttgggggcca gaatgcccac 4561 aatcaaattg ccatttatgc tcaccaaccc cgaactgcag atgaaattcc catggaacct 4621 ggagatatca ttggtgtggc tggaaatcat tgggatggct attctaaagg tgtcaacagg 4681 aaattgggaa ggacgggcct atatccctcc tacaaagttc gagagaagat agaaacggtc 4741 aagtacccca catatcctga ggctgagaaa TAGGACCCAG CTTTCTTGTA CAAAGTGGTT 4801 GATAGCTTGG ATCCAATCAA CCTCTGGATT ACAAAATTTG TGAAAGATTG ACTGGTATTC 4861 TTAACTATGT TGCTCCTTTT ACGCTATGTG GATACGCTGC TTTAATGCCT TTGTATCATG 4921 CTATTGCTTC CCGTATGGCT TTCATTTTCT CCTCCTTGTA TAAATCCTGG TTGCTGTCTC 4981 TTTATGAGGA GTTGTGGCCC GTTGTCAGGC AACGTGGCGT GGTGTGCACT GTGTTTGCTG 5041 ACGCAACCCC CACTGGTTGG GGCATTGCCA CCACCTGTCA GCTCCTTTCC GGGACTTTCG 5101 CTTTCCCCCT CCCTATTGCC ACGGCGGAAC TCATCGCCGC CTGCCTTGCC CGCTGCTGGA 5161 CAGGGGCTCG GCTGTTGGGC ACTGACAATT CCGTGGTGTT GTCGGGGAAG CTGACGTCCT 5221 TTCCATGGCT GCTCGCCTGT GTTGCCACCT GGATTCTGCG CGGGACGTCC TTCTGCTACG 5281 TCCCTTCGGC CCTCAATCCA GCGGACCTTC CTTCCCGCGG CCTGCTGCCG GCTCTGCGGC 5341 CTCTTCCGCG TCTTCGAGAT CTGCCTCGAC TGTGCCTTCT AGTTGCCAGC CATCTGTTGT 5401 TTGCCCCTCC CCCGTGCCTT CCTTGACCCT GGAAGGTGCC ACTCCCACTG TCCTTTCCTA 5461 ATAAAATGAG GAAATTGCAT CGCATTGTCT GAGTAGGTGT CATTCTATTC TGGGGGGTGG 5521 GGTGGGGCAG GACAGCAAGG GGGAGGATTG GGAAGACAAT AGCAGGCATG CTGGGGACTC 5581 GAGTTAAGGG CGAATTGCCG ATTAGGATCT TCCTAGAGCA TGGCTACGTA GATAAGTAGC 5641 ATGGCGGGTT AATCATTAAC TACAAGGAAC CCCTAGTGAT GGAGTTGGCC ACTCCCTCTC 5701 TGCGCGCTCG CTCGCTCACT GAGGCCGGGC GACCAAAGGT CGCCCGACGC CCGGGCTTTG 5761 CCCGGGCGGC CTCAGTGAGC GAGCGAGCGC GCAGCCTTAA TTAAGAGCTC ATGGCGCGCC 5821 TAGGCCTTGA CGGCCTTCCG CCAATTCGCC CTATAGTGAG TCGTATTACG TCGCGCTCAC 5881 TGGCCGTCGT TTTACAACGT CGTGACTGGG AAAACCCTGG CGTTACCCAA CTTAATCGCC 5941 TTGCAGCACA TCCCCCTTTC GCCAGCTGGC GTAATAGCGA AGAGGCCCGC ACCGAAACGC 6001 CCTTCCCAAC AGTTGCGCAG CCTGAATGGC GAATGGGAGC GCCCTGTAGC GGCCACTCAA 6061 CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT 6121 AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT AACAAAATAT TAACGCTTAC 6181 AATTTAG //