LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(5225..5321) /label=attL2 /note="attL2 (c)" misc_signal complement(5336..5355) /note="T7 Promoter/priming site (c)" primer_bind 5363..5379 /note="M13 Reverse priming site" gene 5492..6301 /note="Kanamycin resistance gene" rep_origin 6422..7095 /note="pUC origin" vector join(5233..7098,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 669..691 /note="TEV site" source 692..5218 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:46056 IMAGE:5492334" /tissue_type="Eye, retinoblastoma" /clone_lib="NIH_MGC_67" /lab_host="DH10B" /note="Vector: pCMV-SPORT6" gene 692..5218 /gene="UGCGL1" /gene_synonym=HUGT1 /db_xref="GeneID:56886" /db_xref="HGNC:15663" /db_xref="MIM:605897" CDS 692..5218 /dnas_title="UDP-glucose ceramide glucosyltransferase-like 1" /gene="UGCGL1" /gene_synonym=HUGT1 /codon_start=1 /product="UDP-glucose ceramide glucosyltransferase-like 1" /protein_id="AAH41098.1" /db_xref="GI:26996810" /db_xref="GeneID:56886" /db_xref="HGNC:15663" /db_xref="MIM:605897" /translation="MGVLVVLTVLWLFSSVKADSKAITTSLTTKWFSTPLLLEASEFL AEDSQEKFWNFVEASQNIGSSDHDGTDYSYYHAILEAAFQFLSPLQQNLFKFCLSLRS YSATIQAFQQIAADEPPPEGCNSFFSVHGKKTCESDTLEALLLTASERPKPLLFKGDH RYPSSNPESPVVIFYSEIGSEEFSNFHRQLISKSNAGKINYVFRHYIFNPRKEPVYLS GYGVELAIKSTEYKAKDDTQVKGTEVNTTVIGENDPIDEVQGFLFGKLRDLHPDLEGQ LKELRKHLVESTNEMAPLKVWQLQDLSFQTAARILASPVELALVVMKDLSQNFPTKAR AITKTAVSSELRTEVEENQKYFKGTLGLQPGDSALFINGLHMDLDTQDIFSLFDVLRN EARVMEGLHRLGIEGLSLHNVLKLNIQPSEADYAVDIRSPAISWVNNLEVDSRYNSWP SSLQELLRPTFPGVIRQIRKNLHNMVFIVDPAHETTAELMNTAEMFLSNHIPLRIGFI FVVNDSEDVDGMQDAGVAVLRAYNYVAQEVDDYHAFQTLTHIYNKVRTGEKVKVEHVV SVLEKKYPYVEVNSILGIDSAYDRNRKEARGYYEQTGVGPLPVVLFNGMPFEREQLDP DELETITMHKILETTTFFQRAVYLGELPHDQDVVEYIMNQPNVVPRINSRILTAERDY LDLTASNNFFVDDYARFTILDSQGKTAAVANSMNYLTKKGMSSKEIYDDSFIRPVTFW IVGDFDSPSGRQLLYDAIKHQKSSNNVRISMINNPAKEISYENTQISRAIWAALQTQT SNAAKNFITKMAKEGAAEALAAGADIAEFSVGGMDFSLFKEVFESSKMDFILSHAVYC RDVLKLKKGQRAVISNGRIIGPLEDSELFNQDDFHLLENIILKTSGQKIKSHIQQLRV EEDVASDLVMKVDALLSAQPKGDPRIEYQFFEDRHSAIKLRPKEGETYFDVVAVVDPV TREAQRLAPLLLVLAQLINMNLRVFMNCQSKLSDMPLKSFYRYVLEPEISFTSDNSFA KGPIAKFLDMPQSPLFTLNLNTPESWMVESVRTPYDLDNIYLEEVDSVVAAEYELEYL LLEGHCYDITTGQPPRGLQFTLGTSANPVIVDTIVMANLGYFQLKANPGAWILRLRKG RSEDIYRIYSHDGTDSPPDADEVVIVLNNFKSKIIKVKVQKKADMVNEDLLSDGTSEN ESGFWDSFKWGFTGQKTEEVKQDKDDIINIFSVASGHLYERFLRIMMLSVLKNTKTPV KFWFLKNYLSPTFKEFIPYMANEYNFQYELVQYKWPRWLHQQTEKQRIIWGYKILFLD VLFPLVVDKFLFVDADQIVRTDLKELRDFNLDGAPYGYTPFCDSRREMDGYRFWKSGY WASHLAGRKYHISALYVVDLKKFRKIAAGDRLRGQYQGLSQDPNSLSNLDQDLPNNMI HQVPIKSLPQEWLWCETWCDDASKKRAKTIDLCNNPMTKEPKLEAAVRIVPEWQDYDQ EIKQLQIRFQKEKETGALYKEKTKEPSREGPQKREEL" misc_feature 692..5218 /note="UGCGL1 coding region" misc_difference 1237..1237 /gene="UGCGL1" /gene_synonym=HUGT1 /note="'C' in cDNA is 'T' in the human genome; no amino acid change. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." misc_difference 1522..1522 /gene="UGCGL1" /gene_synonym=HUGT1 /note="'A' in cDNA is 'G' in the human genome; no amino acid change." ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg cgactcaaaa gccattacaa cctctcttac 721 aacaaaatgg ttttccactc cattgttgtt agaagccagt gagtttttag cagaagacag 781 tcaagagaaa ttttggaatt ttgtagaagc cagtcaaaat attggatcat cagatcatga 841 cggtaccgat tattcctact atcatgcaat attggaggct gcatttcagt ttctgtcacc 901 cctccagcag aatttgttta aattttgtct gtcccttcgt tcttactcag ctacaatcca 961 agccttccag cagatagcag ctgatgaacc tccaccagaa ggatgtaatt cgtttttttc 1021 agtgcatgga aagaagactt gtgaatctga tacccttgag gctcttctac tgacagcctc 1081 tgaaagaccc aaacctttat tgttcaaagg agatcacaga tatccctcgt ctaatcctga 1141 aagccctgtg gtgattttct actctgagat tggctctgag gaattttcca attttcaccg 1201 ccagcttata tcaaaaagca atgcaggcaa aatcaactat gtattcagac attatatatt 1261 taatcccagg aaggagcctg tttacctctc tggctatggc gtggaattgg ccattaagag 1321 cactgagtac aaggccaagg atgatactca ggtgaaagga actgaggtaa acaccacagt 1381 gattggtgaa aatgatccta ttgatgaggt tcaggggttc ctctttggaa aattaagaga 1441 tctgcacccc gacctggagg gacagttgaa agaactcaga aagcatcttg tagagagcac 1501 caatgaaatg gcacctttaa aagtttggca gttgcaagat ctcagtttcc agactgctgc 1561 tcgaatcttg gcttctcctg ttgagttggc tttggttgtc atgaaggatc ttagtcagaa 1621 ttttcctacc aaagccagag caataacaaa aacagctgtg agctcagaac ttagaaccga 1681 agtggaagag aatcagaagt atttcaaggg aactttagga ttacaacctg gagattcagc 1741 cctcttcatc aatggacttc acatggattt agatacacag gatatattca gtctgtttga 1801 tgtgttgagg aatgaagctc gggtaatgga gggtctgcat agattgggaa tagaaggcct 1861 ttctctgcat aatgttttga agctgaacat ccagccctct gaggcagact atgccgtaga 1921 catccggagt cctgctattt catgggtcaa caacctggag gttgatagca gatataattc 1981 gtggccttct agtttacaag agttgcttcg acccaccttt cctggtgtta ttcggcagat 2041 caggaaaaac ttacataata tggttttcat agttgatcct gcacatgaga ccacagcaga 2101 gttgatgaac acagctgaga tgttccttag taatcatata ccactaagaa ttggttttat 2161 ctttgtggtt aatgactctg aagatgttga tgggatgcaa gatgctggag tggctgttct 2221 tagagcatat aattatgttg cccaagaagt ggatgattat catgccttcc agactctgac 2281 acatatctat aacaaggtga ggactggaga aaaagtgaaa gttgaacatg tggtcagtgt 2341 cctggagaag aaatatccgt atgtagaagt gaatagcatt ttggggattg attctgctta 2401 tgatcggaat cggaaggaag caagaggcta ctatgagcag actggagttg gacctctgcc 2461 cgttgtgctg ttcaatggaa tgccctttga aagggaacag ctagaccctg atgagttaga 2521 aaccatcaca atgcataaaa tcctggagac caccaccttc ttccaaagag cggtgtactt 2581 gggtgaactg ccccatgatc aagatgtggt agagtatatc atgaatcagc caaatgttgt 2641 tccacgaatc aattctagga ttttgacagc tgaacgagac tacctggatt taacagcgag 2701 taataacttc tttgtggatg attatgctag atttactatc ttggattccc aaggcaagac 2761 tgctgctgta gccaatagta tgaactatct gacaaagaaa ggaatgtcct ccaaggaaat 2821 ctatgatgat tcttttatta ggccagtaac tttttggatt gttggggatt ttgatagccc 2881 ttctggacgg cagttactgt atgatgccat caaacatcag aaatccagta acaatgttag 2941 aataagcatg atcaataatc ctgccaaaga gataagctat gagaacactc agatctccag 3001 agcaatctgg gcagctctcc aaactcagac ttccaacgct gctaagaact tcatcaccaa 3061 aatggccaag gagggggctg cagaggccct ggctgcagga gctgacattg cggagttctc 3121 tgttggggga atggatttca gtctttttaa agaggtcttt gagtcttcca aaatggattt 3181 cattttgtct catgccgtgt actgcaggga tgttctgaag ctgaagaagg gacagagggc 3241 agtgatcagc aatggaagga tcattgggcc actggaggat agtgagctct ttaatcaaga 3301 cgatttccac ctcctcgaaa atatcatctt aaaaacctca ggacagaaaa taaaatctca 3361 tattcaacag cttcgggtag aagaagatgt ggcaagcgac ttggtaatga aggtggatgc 3421 tcttctgtca gcgcaaccaa aaggagatcc aagaatcgag taccagtttt ttgaagacag 3481 acacagtgca atcaaactga ggccgaagga aggggagaca tactttgatg ttgtggctgt 3541 cgttgaccct gtcaccagag aagcacagag acttgctcct ttgctcttgg ttttggctca 3601 gctgataaac atgaatctga gagtatttat gaactgccaa tccaaacttt ctgacatgcc 3661 tttaaaaagc ttttaccgtt atgtcttaga accagagatt tctttcactt cagacaatag 3721 ttttgctaag ggtccaatcg caaaattttt ggatatgcct cagtctccac tgttcactct 3781 gaatttgaac acacctgaga gctggatggt agaatctgtc agaacaccat atgatcttga 3841 taatatttat ttagaagagg tggacagtgt agtggctgct gagtatgagc tggaatacct 3901 gttactggaa ggtcattgct acgacatcac cacaggccag cctccacggg gactacagtt 3961 taccttagga acttcagcca acccggtcat tgtggacacc attgttatgg ccaatctggg 4021 ctactttcag ctgaaagcca acccaggagc ttggatcctc agacttagga agggacgctc 4081 tgaagatatt tatagaattt acagccacga tggcactgat tctccccctg atgctgatga 4141 ggtggttatc gtcctcaaca acttcaaaag caaaattatt aaagtgaagg ttcagaagaa 4201 ggcagatatg gtgaacgaag acttgctgag tgatggaacg agtgagaatg aatctggatt 4261 ttgggattcc ttcaaatggg gctttacagg acagaagact gaggaagtga agcaagataa 4321 agatgacata attaatattt tctccgttgc atctggtcat ctctacgaaa gatttcttcg 4381 cataatgatg ctatccgtgc tgaagaatac caagactcct gtgaaattct ggttcttgaa 4441 gaattacttg tcccccacat ttaaggagtt tataccttac atggcaaatg aatacaattt 4501 ccagtatgag cttgttcagt acaaatggcc ccggtggctt catcaacaaa ctgaaaaaca 4561 gcgtatcatc tggggttaca agatcctctt cctggatgta cttttcccac tagttgttga 4621 caagttcctg tttgtggatg ctgatcagat tgtacgaaca gatctgaaag agttaagaga 4681 tttcaatttg gatggtgctc cttatggtta cactcctttc tgtgacagcc gaagagaaat 4741 ggacggctac aggttctgga agtcagggta ctgggccagt catttagccg ggcgaaagta 4801 tcatatcagt gcactatatg ttgtggatct gaagaagttt aggaaaatag ctgctggtga 4861 cagactcagg ggacagtacc aaggtctgag tcaggaccct aacagccttt caaatcttga 4921 tcaagatctg cccaataaca tgattcatca ggtgccaatt aaatccctcc ctcaagaatg 4981 gctttggtgt gaaacgtggt gtgatgacgc ctctaagaaa agggcaaaaa ccattgattt 5041 gtgtaataat ccgatgacca aagagccgaa actggaagca gctgtgcgga ttgtcccgga 5101 gtggcaggac tacgaccaag agatcaaaca gctacagatc cgctttcaga aggagaaaga 5161 aacgggagca ctgtacaaag agaagacaaa agaaccaagc cgagaaggtc ctcagaaaTA 5221 GGACCCAGCT TTCTTGTACA AAGTTGGCAT TATAAGAAAG CATTGCTTAT CAATTTGTTG 5281 CAACGAACAG GTCACTATCA GTCAAAATAA AATCATTATT TGCCATCCAG CTGATATCCC 5341 CTATAGTGAG TCGTATTACA TGGTCATAGC TGTTTCCTGG CAGCTCTGGC CCGTGTCTCA 5401 AAATCTCTGA TGTTACATTG CACAAGATAA AATAATATCA TCATGAACAA TAAAACTGTC 5461 TGCTTACATA AACAGTAATA CAAGGGGTGT TATGAGCCAT ATTCAACGGG AAACGTCGAG 5521 GCCGCGATTA AATTCCAACA TGGATGCTGA TTTATATGGG TATAAATGGG CTCGCGATAA 5581 TGTCGGGCAA TCAGGTGCGA CAATCTATCG CTTGTATGGG AAGCCCGATG CGCCAGAGTT 5641 GTTTCTGAAA CATGGCAAAG GTAGCGTTGC CAATGATGTT ACAGATGAGA TGGTCAGACT 5701 AAACTGGCTG ACGGAATTTA TGCCTCTTCC GACCATCAAG CATTTTATCC GTACTCCTGA 5761 TGATGCATGG TTACTCACCA CTGCGATCCC CGGAAAAACA GCATTCCAGG TATTAGAAGA 5821 ATATCCTGAT TCAGGTGAAA ATATTGTTGA TGCGCTGGCA GTGTTCCTGC GCCGGTTGCA 5881 TTCGATTCCT GTTTGTAATT GTCCTTTTAA CAGCGATCGC GTATTTCGTC TCGCTCAGGC 5941 GCAATCACGA ATGAATAACG GTTTGGTTGA TGCGAGTGAT TTTGATGACG AGCGTAATGG 6001 CTGGCCTGTT GAACAAGTCT GGAAAGAAAT GCATAAACTT TTGCCATTCT CACCGGATTC 6061 AGTCGTCACT CATGGTGATT TCTCACTTGA TAACCTTATT TTTGACGAGG GGAAATTAAT 6121 AGGTTGTATT GATGTTGGAC GAGTCGGAAT CGCAGACCGA TACCAGGATC TTGCCATCCT 6181 ATGGAACTGC CTCGGTGAGT TTTCTCCTTC ATTACAGAAA CGGCTTTTTC AAAAATATGG 6241 TATTGATAAT CCTGATATGA ATAAATTGCA GTTTCATTTG ATGCTCGATG AGTTTTTCTA 6301 ATCAGAATTG GTTAATTGGT TGTAACACTG GCAGAGCATT ACGCTGACTT GACGGGACGG 6361 CGCAAGCTCA TGACCAAAAT CCCTTAACGT GAGTTACGCG TCGTTCCACT GAGCGTCAGA 6421 CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG 6481 CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC 6541 AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTTCTTCT 6601 AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC 6661 TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT 6721 GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG 6781 CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT 6841 ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG 6901 GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG 6961 TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG 7021 GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG 7081 GCCTTTTGCT CACATGTT //