LOCUS GALNT10-pGEn3-DE 7653 bp DNA circular 22-JAN-2013 FEATURES Location/Qualifiers CDS 129..1108 /dnas_title="AmpR" /label=AmpR /vntifkey="4" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_recomb complement(4541..4569) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_feature 6281..6822 /note="WPRE" misc_recomb 6243..6265 /label=attB2 /note="attB2 site" /note="recognized as attB2" misc_feature complement(6829..7043) /note="BGH pA" misc_feature complement(7131..7260) /note="3'-ITR" misc_feature 2982..3056 /note="TCM signal sequence" misc_feature 3060..3083 /note="His tag" misc_feature 3084..3134 /note="AviTag" misc_feature 3135..3830 /note="Superfolder GFP" misc_feature 3837..4532 /note="Fc domain" misc_feature 2976..2981 /note="Kozak seq" misc_feature 4570..4592 /note="TEV site" source 4593..6236 /dnas_title="GALNT10-pGEn3-DEST" /organism="synthetic construct" /mol_type="other DNA" /db_xref="taxon:32630" /clone="MGC:184321 IMAGE:100016589" /clone_lib="NIH_MGC_428" /lab_host="DH10B" /focus /note="Vector: pENTR223.1 with stop codon" source 4593..6236 /organism="Homo sapiens" /mol_type="other DNA" /db_xref="taxon:9606" gene 4593..6236 /dnas_title="GALNT10" /gene="GALNT10" /gene_synonym="DKFZp586H0623" /gene_synonym="GalNAcT10" /gene_synonym="pp-GalNAc-T10" /db_xref="GeneID:55568" /db_xref="HGNC:19873" /db_xref="MIM:608043" CDS 4593..6236 /dnas_title="UDP-N-acetyl-alpha-D-galactosamine:polypeptid e N-acetylgalactosaminyltransferase 10 (GalNAc-T10)" /gene="GALNT10" /gene_synonym="DKFZp586H0623" /gene_synonym="GalNAcT10" /gene_synonym="pp-GalNAc-T10" /codon_start=1 /product="UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 10 (GalNAc-T10)" /protein_id="AAI53182.1" /db_xref="GI:157169754" /db_xref="GeneID:55568" /db_xref="HGNC:19873" /db_xref="MIM:608043" /translation="MRRKEKRLLQAVALVLAALVLLPNVGLWALYRERQPDGTPGGSG AAVAPAAGQGSHSRQKKTFFLGDGQKLKDWHDKEAIRRDAQRVGNGEQGRPYPMTDAE RVDQAYRENGFNIYVSDKISLNRSLPDIRHPNCNSKRYLETLPNTSIIIPFHNEGWSS LLRTVHSVLNRSPPELVAEIVLVDDFSDREHLKKPLEDYMALFPSVRILRTKKREGLI RTRMLGASVATGDVITFLDSHCEANVNWLPPLLDRIARNRKTIVCPMIDVIDHDDFRY ETQAGDAMRGAFDWEMYYKRIPIPPELQKADPSDPFESPVMAGGLFAVDRKWFWELGG YDPGLEIWGGEQYEISFKVWMCGGRMEDIPCSRVGHIYRKYVPYKVPAGVSLARNLKR VAEVWMDEYAEYIYQRRPEYRHLSAGDVAVQKKLRSSLNCKSFKWFMTKIAWDLPKFY PPVEPPAAAWGEIRNVGTGLCADTKHGALGSPLRLEGCVRGRGEAAWNNMQVFTFTWR EDIRPGDPQHTKKFCFDAISHTSPVTLYDCHSMKGNQLWKYRKDKTLYHPVSGSCMDC SESDHRIFMNTCNPSSLTQQWLFEHTNSTVLEKFNRN" misc_feature 4593..6236 /note="GALNT10 coding region" vector join(6251..7653,1..4559) /source="pGEn3-DEST" /type="Destination Vector" clone 4560..6250 /source="GALNT10-Nterm-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAgcg gccgcGCCAC cATGAGACTG CTGACCGCCC 3001 TGTTCGCCTA CTTCATCGTG GCCCTGATCC TGGCCTTCAG CGTGTCCGCC AAGAGCATGC 3061 ACCACCACCA TCACCATCAT CACATGAGCG GCCTGAACGA CATCTTCGAG GCCCAGAAAA 3121 TCGAGTGGCA CGAGATGAGC AAGGGCGAGG AACTGTTCAC CGGCGTGGTG CCCATCCTGG 3181 TGGAACTGGA CGGCGACGTG AACGGCCACA AGTTCTCTGT GCGGGGCGAG GGCGAGGGGG 3241 ACGCCACAAA TGGCAAGCTG ACCCTGAAGT TCATCTGCAC CACCGGAAAG CTGCCCGTGC 3301 CCTGGCCTAC CCTGGTCACA ACCCTGACCT ACGGCGTGCA GTGCTTCAGC AGATACCCCG 3361 ACCACATGAA GCGGCACGAT TTCTTCAAGA GCGCCATGCC CGAGGGCTAC GTGCAAGAAC 3421 GGACCATCAG CTTCAAGGAC GACGGCACCT ACAAGACCAG AGCCGAAGTG AAGTTCGAGG 3481 GCGACACCCT GGTCAACCGG ATCGAGCTGA AGGGCATCGA CTTCAAAGAG GACGGCAACA 3541 TCCTGGGCCA CAAGCTCGAG TACAACTTCA ACAGCCACAA CGTGTACATC ACCGCCGACA 3601 AGCAGAAGAA CGGCATCAAG GCCAACTTCA AGATCCGGCA CAACGTGGAA GATGGCAGCG 3661 TGCAGCTGGC CGACCACTAC CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC 3721 CCGACAATCA CTACCTGAGC ACCCAGAGCG TGCTGAGCAA GGACCCCAAC GAGAAGCGGG 3781 ACCACATGGT GCTGCTGGAA TTTGTGACCG CCGCTGGCAT CACCCACGGC GAATTCgagc 3841 ccaaatcttg tgacaaaact cacacatgcc caccgtgccc agcacctgaa ctcctggggg 3901 gaccgtcagt cttcctcttc cccccaaaac ccaaggacac cctcatgatc tcccggaccc 3961 ctgaggtcac atgcgtggtg gtggacgtga gccacgaaga ccctgaggtc aagttcaact 4021 ggtacgtgga cggcgtggag gtgcataatg ccaagacaaa gccgcgggag gagcagtaca 4081 acagcacgta ccgtgtggtc agcgtcctca ccgtcctgca ccaggactgg ctgaatggca 4141 aggagtacaa gtgcaaggtc tccaacaaag ccctcccagc ccccatcgag aaaaccatct 4201 ccaaagccaa agggcagccc cgagaaccac aggtgtacac cctgccccca tcccgggatg 4261 agctgaccaa gaaccaggtc agcctgacct gcctggtcaa aggcttctat cccagcgaca 4321 tcgccgtgga gtgggagagc aatgggcagc cggagaacaa ctacaagacc acgcctcccg 4381 tgctggactc cgacggctcc ttcttcctct acagcaagct caccgtggac aagagcaggt 4441 ggcagcaggg gaacgtcttc tcatgctccg tgatgcatga ggctctgcac aaccactaca 4501 cgcagaagag cctctccctg tctccgggta aagaattcgc ATCAACAAGT TTGTACAAAA 4561 AAGCAGGCTc tgaaaacttg tactttcaag gccacagtcg acaaaagaaa acgtttttct 4621 tgggagatgg gcagaagctg aaggactggc atgacaagga ggccatccgg agggacgctc 4681 agcgcgtagg aaatggagaa caaggaagac cttaccccat gaccgatgct gagagagtgg 4741 atcaggcata ccgagaaaat ggatttaaca tctacgtcag tgataaaatc tccttgaatc 4801 gctctctccc agatatccgg cacccaaact gcaacagcaa gcgctacctg gagacacttc 4861 ccaacacaag catcatcatc cccttccaca acgagggctg gtcctccctc ctccgcaccg 4921 tccacagtgt gctcaatcgc tcgcctccag agctggtcgc cgagattgta ctggtcgacg 4981 acttcagtga tcgagagcac ctgaagaagc ctcttgaaga ctacatggcc cttttcccca 5041 gtgtgaggat tcttcgaacc aagaaacggg aagggctgat aaggacccga atgctggggg 5101 cctcagtggc aactggggat gtcatcacat tcttggattc acactgtgaa gccaatgtca 5161 actggcttcc ccccttgctt gaccgcattg ctcggaaccg caagaccatt gtgtgcccga 5221 tgattgatgt aattgaccat gacgactttc ggtacgagac acaggcaggg gatgccatgc 5281 ggggagcctt tgactgggag atgtactaca agcggatccc gatccctcca gaactgcaga 5341 aagctgaccc cagcgaccca tttgagtctc ccgtgatggc cggtggactg ttcgccgtgg 5401 atcggaagtg gttctgggaa ctcggcgggt atgacccagg cttggagatc tggggagggg 5461 agcagtatga aatctccttc aaggtgtgga tgtgtggggg ccgcatggag gacatcccct 5521 gctccagggt gggccatatc tacaggaagt atgtgcccta caaggtcccg gccggagtca 5581 gcctggcccg gaaccttaag cgggtggccg aagtgtggat ggatgagtac gcagagtaca 5641 tttaccagcg ccggcctgaa taccgccacc tctccgctgg ggatgtcgca gtccagaaaa 5701 agctccgcag ctcccttaac tgcaagagtt tcaagtggtt tatgacgaag atagcctggg 5761 acctgcccaa attctaccca cccgtggagc ccccggctgc agcttggggg gagatccgaa 5821 atgtgggcac agggctgtgt gcagacacaa agcacggggc cttgggctcc ccactaaggc 5881 tagagggctg cgtccgaggc cgtggggagg ctgcctggaa caacatgcag gtattcacct 5941 tcacctggag agaggacatc cggcctggag acccccagca caccaagaag ttctgctttg 6001 atgccatttc ccacaccagc cctgtcacgc tgtacgactg ccacagcatg aagggcaacc 6061 agctgtggaa ataccgcaaa gacaagaccc tgtaccaccc tgtcagtggc agctgcatgg 6121 actgcagtga aagtgaccat aggatcttca tgaacacctg caacccatcc tctctcaccc 6181 agcagtggct gtttgaacac accaactcaa cagtcttgga aaaattcaat aggaacTAGG 6241 ACCCAGCTTT CTTGTACAAA GTGGTTGATA GCTTGGATCC AATCAACCTC TGGATTACAA 6301 AATTTGTGAA AGATTGACTG GTATTCTTAA CTATGTTGCT CCTTTTACGC TATGTGGATA 6361 CGCTGCTTTA ATGCCTTTGT ATCATGCTAT TGCTTCCCGT ATGGCTTTCA TTTTCTCCTC 6421 CTTGTATAAA TCCTGGTTGC TGTCTCTTTA TGAGGAGTTG TGGCCCGTTG TCAGGCAACG 6481 TGGCGTGGTG TGCACTGTGT TTGCTGACGC AACCCCCACT GGTTGGGGCA TTGCCACCAC 6541 CTGTCAGCTC CTTTCCGGGA CTTTCGCTTT CCCCCTCCCT ATTGCCACGG CGGAACTCAT 6601 CGCCGCCTGC CTTGCCCGCT GCTGGACAGG GGCTCGGCTG TTGGGCACTG ACAATTCCGT 6661 GGTGTTGTCG GGGAAGCTGA CGTCCTTTCC ATGGCTGCTC GCCTGTGTTG CCACCTGGAT 6721 TCTGCGCGGG ACGTCCTTCT GCTACGTCCC TTCGGCCCTC AATCCAGCGG ACCTTCCTTC 6781 CCGCGGCCTG CTGCCGGCTC TGCGGCCTCT TCCGCGTCTT CGAGATCTGC CTCGACTGTG 6841 CCTTCTAGTT GCCAGCCATC TGTTGTTTGC CCCTCCCCCG TGCCTTCCTT GACCCTGGAA 6901 GGTGCCACTC CCACTGTCCT TTCCTAATAA AATGAGGAAA TTGCATCGCA TTGTCTGAGT 6961 AGGTGTCATT CTATTCTGGG GGGTGGGGTG GGGCAGGACA GCAAGGGGGA GGATTGGGAA 7021 GACAATAGCA GGCATGCTGG GGACTCGAGT TAAGGGCGAA TTGCCGATTA GGATCTTCCT 7081 AGAGCATGGC TACGTAGATA AGTAGCATGG CGGGTTAATC ATTAACTACA AGGAACCCCT 7141 AGTGATGGAG TTGGCCACTC CCTCTCTGCG CGCTCGCTCG CTCACTGAGG CCGGGCGACC 7201 AAAGGTCGCC CGACGCCCGG GCTTTGCCCG GGCGGCCTCA GTGAGCGAGC GAGCGCGCAG 7261 CCTTAATTAA GAGCTCATGG CGCGCCTAGG CCTTGACGGC CTTCCGCCAA TTCGCCCTAT 7321 AGTGAGTCGT ATTACGTCGC GCTCACTGGC CGTCGTTTTA CAACGTCGTG ACTGGGAAAA 7381 CCCTGGCGTT ACCCAACTTA ATCGCCTTGC AGCACATCCC CCTTTCGCCA GCTGGCGTAA 7441 TAGCGAAGAG GCCCGCACCG AAACGCCCTT CCCAACAGTT GCGCAGCCTG AATGGCGAAT 7501 GGGAGCGCCC TGTAGCGGCC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 7561 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 7621 AATTTTAACA AAATATTAAC GCTTACAATT TAG //