LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2264..2360) /label=attL2 /note="attL2 (c)" misc_signal complement(2375..2394) /note="T7 Promoter/priming site (c)" primer_bind 2402..2418 /note="M13 Reverse priming site" gene 2531..3340 /note="Kanamycin resistance gene" rep_origin 3461..4134 /note="pUC origin" vector join(2272..4137,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 669..691 /note="TEV site" source 692..2257 /organism="synthetic construct" /mol_type="other DNA" /db_xref="taxon:32630" /clone="MGC:183163 IMAGE:100015747" /clone_lib="NIH_MGC_428" /lab_host="DH10B" /focus /note="Vector: pENTR223.1 with stop codon" source 692..2257 /organism="Homo sapiens" /mol_type="other DNA" /db_xref="taxon:9606" gene 692..2257 /gene="GALNT12" /gene_synonym=GalNAc-T12 /db_xref="GeneID:79695" /db_xref="HGNC:19877" /db_xref="MIM:610290" CDS 692..2257 /dnas_title="UDP-N-acetyl-alpha-D-galactosamine:polypeptid e N-acetylgalactosaminyltransferase 12 (GalNAc-T12)" /gene="GALNT12" /gene_synonym=GalNAc-T12 /codon_start=1 /product="UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 12 (GalNAc-T12)" /protein_id="AAI48740.1" /db_xref="GI:151555601" /db_xref="GeneID:79695" /db_xref="HGNC:19877" /db_xref="MIM:610290" /translation="MWGRTARRRCPRELRRGREALLVLLALLALAGLGSVLRAQRGAG AGAAEPGPPRTPRPGRREPVMPRPPVPANALGARGEAVRLQLQGEELRLQEESVRLHQ INIYLSDRISLHRRLPERWNPLCKEKKYDYDNLPRTSVIIAFYNEAWSTLLRTVYSVL ETSPDILLEEVILVDDYSDREHLKERLANELSGLPKVRLIRANKREGLVRARLLGASA ARGDVLTFLDCHCECHEGWLEPLLQRIHEEESAVVCPVIDVIDWNTFEYLGNSGEPQI GGFDWRLVFTWHTVPERERIRMQSPVDVIRSPTMAGGLFAVSKKYFEYLGSYDTGMEV WGGENLEFSFRIWQCGGVLETHPCSHVGHVFPKQAPYSRNKALANSVRAAEVWMDEFK ELYYHRNPRARLEPFGDVTERKQLRDKLQCKDFKWFLETVYPELHVPEDRPGFFGMLQ NKGLTDYCFDYNPPDENQIVGHQVILYLCHGMGQNQFFEYTSQKEIRYNTHQPEGCIA VEAGMDTLIMHLCEETAPENQKFILQEDGSLFHEQSKKCVQAARKESSDSFVPLLRDC TNSDHQKWFFKERML" misc_feature 692..2257 /note="GALNT12 coding region" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg ccggcgcgag ccggtcatgc cgcggccgcc 721 ggtgccggcg aacgcgctgg gcgcgcgggg cgaggcggtg cggctgcagc tgcagggcga 781 ggagctgcgg ctgcaggagg agagcgtgcg gctgcaccag attaacatct acctcagcga 841 ccgcatctca ctgcaccgcc gcctgcccga gcgctggaac ccgctgtgca aagagaagaa 901 atatgattat gataatttgc ccaggacatc tgttatcata gcattttata atgaagcctg 961 gtcaactctc cttcggacag tttacagtgt ccttgagaca tccccggata tcctgctaga 1021 agaagtgatc cttgtagatg actacagtga tagagagcac ctgaaggagc gcttggccaa 1081 tgagctttcg ggactgccca aggtgcgcct gatccgcgcc aacaagagag agggcctggt 1141 gcgagcccgg ctgctggggg cgtctgcggc gaggggcgat gttctgacct tcctggactg 1201 tcactgtgag tgccacgaag ggtggctgga gccgctgctg cagaggatcc atgaagagga 1261 gtcggcagtg gtgtgcccgg tgattgatgt gatcgactgg aacaccttcg aatacctggg 1321 gaactccggg gagccccaga tcggcggttt cgactggagg ctggtgttca cgtggcacac 1381 agttcctgag agggagagga tacggatgca atcccccgtc gatgtcatca ggtctccaac 1441 aatggctggt gggctgtttg ctgtgagtaa gaaatatttt gaatatctgg ggtcttatga 1501 tacaggaatg gaagtttggg gaggagaaaa cctcgaattt tcctttagga tctggcagtg 1561 tggtggggtt ctggaaacac acccatgttc ccatgttggc catgttttcc ccaagcaagc 1621 tccctactcc cgcaacaagg ctctggccaa cagtgttcgt gcagctgaag tatggatgga 1681 tgaatttaaa gagctctact accatcgcaa cccccgtgcc cgcttggaac cttttgggga 1741 tgtgacagag aggaagcagc tccgggacaa gctccagtgt aaagacttca agtggttctt 1801 ggagactgtg tatccagaac tgcatgtgcc tgaggacagg cctggcttct tcgggatgct 1861 ccagaacaaa ggactaacag actactgctt tgactataac cctcccgatg aaaaccagat 1921 tgtgggacac caggtcattc tgtacctctg tcatgggatg ggccagaatc agtttttcga 1981 gtacacgtcc cagaaagaaa tacgctataa cacccaccag cctgagggct gcattgctgt 2041 ggaagcagga atggataccc ttatcatgca tctctgcgaa gaaactgccc cagagaatca 2101 gaagttcatc ttgcaggagg atggatcttt atttcacgaa cagtccaaga aatgtgtcca 2161 ggctgcgagg aaggagtcga gtgacagttt cgttccactc ttacgagact gcaccaactc 2221 ggatcatcag aaatggttct tcaaagagcg catgttaTAG GACCCAGCTT TCTTGTACAA 2281 AGTTGGCATT ATAAGAAAGC ATTGCTTATC AATTTGTTGC AACGAACAGG TCACTATCAG 2341 TCAAAATAAA ATCATTATTT GCCATCCAGC TGATATCCCC TATAGTGAGT CGTATTACAT 2401 GGTCATAGCT GTTTCCTGGC AGCTCTGGCC CGTGTCTCAA AATCTCTGAT GTTACATTGC 2461 ACAAGATAAA ATAATATCAT CATGAACAAT AAAACTGTCT GCTTACATAA ACAGTAATAC 2521 AAGGGGTGTT ATGAGCCATA TTCAACGGGA AACGTCGAGG CCGCGATTAA ATTCCAACAT 2581 GGATGCTGAT TTATATGGGT ATAAATGGGC TCGCGATAAT GTCGGGCAAT CAGGTGCGAC 2641 AATCTATCGC TTGTATGGGA AGCCCGATGC GCCAGAGTTG TTTCTGAAAC ATGGCAAAGG 2701 TAGCGTTGCC AATGATGTTA CAGATGAGAT GGTCAGACTA AACTGGCTGA CGGAATTTAT 2761 GCCTCTTCCG ACCATCAAGC ATTTTATCCG TACTCCTGAT GATGCATGGT TACTCACCAC 2821 TGCGATCCCC GGAAAAACAG CATTCCAGGT ATTAGAAGAA TATCCTGATT CAGGTGAAAA 2881 TATTGTTGAT GCGCTGGCAG TGTTCCTGCG CCGGTTGCAT TCGATTCCTG TTTGTAATTG 2941 TCCTTTTAAC AGCGATCGCG TATTTCGTCT CGCTCAGGCG CAATCACGAA TGAATAACGG 3001 TTTGGTTGAT GCGAGTGATT TTGATGACGA GCGTAATGGC TGGCCTGTTG AACAAGTCTG 3061 GAAAGAAATG CATAAACTTT TGCCATTCTC ACCGGATTCA GTCGTCACTC ATGGTGATTT 3121 CTCACTTGAT AACCTTATTT TTGACGAGGG GAAATTAATA GGTTGTATTG ATGTTGGACG 3181 AGTCGGAATC GCAGACCGAT ACCAGGATCT TGCCATCCTA TGGAACTGCC TCGGTGAGTT 3241 TTCTCCTTCA TTACAGAAAC GGCTTTTTCA AAAATATGGT ATTGATAATC CTGATATGAA 3301 TAAATTGCAG TTTCATTTGA TGCTCGATGA GTTTTTCTAA TCAGAATTGG TTAATTGGTT 3361 GTAACACTGG CAGAGCATTA CGCTGACTTG ACGGGACGGC GCAAGCTCAT GACCAAAATC 3421 CCTTAACGTG AGTTACGCGT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG 3481 ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC 3541 GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC 3601 TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTTCTTCTA GTGTAGCCGT AGTTAGGCCA 3661 CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT 3721 GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC 3781 GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG 3841 AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC 3901 CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC 3961 GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT 4021 CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC 4081 CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTT //