LOCUS GALNT4-pGEn1-DES 6178 bp DNA circular 22-JAN-2013 FEATURES Location/Qualifiers CDS 129..1108 /dnas_title="AmpR" /label=AmpR /vntifkey="4" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_feature 2977..3054 /note="TCM Sig Seq" misc_recomb complement(3114..3142) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_feature 3055..3078 /note="8xHis Tag" misc_feature 3085..3108 /note="StrepTag" misc_feature 4806..5347 /note="WPRE" misc_recomb 4768..4790 /label=attB2 /note="attB2 site" /note="recognized as attB2" misc_feature complement(5354..5568) /note="BGH pA" misc_feature complement(5656..5785) /note="3'-ITR" misc_feature 3143..3165 /note="TEV site" source 3166..4761 /dnas_title="GALNT4-pGEn1-DEST" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:41784 IMAGE:5261236" /tissue_type="Brain, hippocampus" /clone_lib="NIH_MGC_95" /lab_host="DH10B" /note="Vector: pBluescriptR" gene 3166..4761 /dnas_title="GALNT4" /gene="GALNT4" /gene_synonym="GALNAC-T4" /gene_synonym="GalNAcT4" /db_xref="GeneID:8693" /db_xref="HGNC:4126" /db_xref="MIM:603565" CDS 3166..4761 /dnas_title="UDP-N-acetyl-alpha-D-galactosamine:polypeptid e N-acetylgalactosaminyltransferase 4 (GalNAc-T4)" /gene="GALNT4" /gene_synonym="GALNAC-T4" /gene_synonym="GalNAcT4" /codon_start=1 /product="UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 4 (GalNAc-T4)" /protein_id="AAH36390.1" /db_xref="GI:22137798" /db_xref="GeneID:8693" /db_xref="HGNC:4126" /db_xref="MIM:603565" /translation="MAVRWTWAGKSCLLLAFLTVAYIFVELLVSTFHASAGAGRAREL GSRRLSGLQKNTEDLSRPLYKKPPADSRALGEWGKASKLQLNEDELKQQEELIERYAI NIYLSDRISLHRHIEDKRMYECKSQKFNYRTLPTTSVIIAFYNEAWSTLLRTIHSVLE TSPAVLLKEIILVDDLSDRVYLKTQLETYISNLDRVRLIRTNKREGLVRARLIGATFA TGDVLTFLDCHCECNSGWLEPLLERIGRDETAVVCPVIDTIDWNTFEFYMQIGEPMIG GFDWRLTFQWHSVPKQERDRRISRIDPIRSPTMAGGLFAVSKKYFQYLGTYDTGMEVW GGENLELSFRVWQCGGKLEIHPCSHVGHVFPKRAPYARPNFLQNTARAAEVWMDEYKE HFYNRNPPARKEAYGDISERKLLRERLRCKSFDWYLKNVFPNLHVPEDRPGWHGAIRS RGISSECLDYNSPDNNPTGANLSLFGCHGQGGNQFFEYTSNKEIRFNSVTELCAEVPE QKNYVGMQNCPKDGFPVPANIIWHFKEDGTIFHPHSGLCLSAYRTPEGRPDVQMRTCD ALDKNQIWSFEK" misc_difference 3179..3179 /dnas_title="GALNT4" /gene="GALNT4" /gene_synonym="GALNAC-T4" /gene_synonym="GalNAcT4" /note="'G' in cDNA is 'A' in the human genome; amino acid difference: 'G' in cDNA, 'D' in the human genome. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." misc_feature 3166..4761 /note="GALNT4 coding region" vector join(4776..6178,1..3132) /source="pGEn1-DEST" /type="Destination Vector" clone 3133..4775 /source="GALNT4-Nterm-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCGCCATGA GACTGCTGAC AGCCCTGTTC 3001 GCCTACTTCA TCGTGGCCCT GATCCTGGCC TTCAGCGTGT CCGCCAAGAG CATGCACCAC 3061 CACCATCACC ATCATCACAG CGCCTGGTCC CACCCCCAGT TCGAGAAAGA ATTATCAACA 3121 AGTTTGTACA AAAAAGCAGG CTctgaaaac ttgtactttc aaggcagaag gctctcaggc 3181 ctccagaaaa atacggagga tttgtctcga ccgctttata agaagccccc tgcagattcc 3241 cgtgcacttg gggagtgggg gaaagccagc aaactccagc tcaacgagga tgaactgaag 3301 cagcaagaag aactcattga gagatacgcc atcaatattt acctcagtga caggatttcc 3361 ctgcatcgac acatagagga taaaagaatg tatgagtgta agtcccagaa gttcaactat 3421 aggacacttc ctaccacctc tgttatcatt gctttctata acgaagcctg gtcgactttg 3481 ctccgtacca ttcacagtgt tttagaaact tctcctgcag ttcttttgaa agagatcatc 3541 ttggtggatg acttgagtga cagagtttat ttgaagacac aacttgaaac ttacatcagc 3601 aatcttgata gagtacgctt gattaggacc aataagcgag aggggctggt tagggcccgt 3661 ctgattgggg ccactttcgc cactggggac gtcctcactt tcctggattg tcactgtgag 3721 tgtaattccg gttggctgga accgcttttg gaaaggattg ggagagatga aacagcagtt 3781 gtgtgtcctg ttatagacac aattgattgg aatacttttg aattctatat gcagataggg 3841 gagcccatga ttggtgggtt tgactggcgt ttaacatttc agtggcattc tgtccccaaa 3901 caggaaaggg acaggcggat atcaagaatt gaccccatca gatcacctac catggctgga 3961 ggactgtttg ctgtcagcaa gaaatatttt cagtaccttg gaacgtatga cacaggaatg 4021 gaagtgtggg gaggtgaaaa ccttgagctg tcttttaggg tgtggcagtg tggtggcaaa 4081 ttggagatcc acccgtgttc ccacgtgggc catgtgttcc ccaagcgggc accatatgct 4141 cgccccaatt tcctacagaa tactgctcgg gcagcagaag tttggatgga tgaatacaaa 4201 gagcacttct acaatagaaa ccctccagca agaaaagaag cttatggtga tatttctgaa 4261 agaaaattac tacgagagcg gttgagatgc aagagctttg actggtattt gaaaaacgtt 4321 tttcctaatt tacatgttcc agaggataga ccaggctggc atggggctat tcgcagtaga 4381 gggatctcgt ctgaatgttt agattataat tctcctgaca acaaccccac aggtgctaac 4441 ctttcactgt ttggatgcca tggtcaagga ggcaatcaat tctttgaata tacttcaaac 4501 aaagaaataa ggtttaattc tgtgacagag ttatgtgcag aggtacctga gcaaaaaaat 4561 tatgtgggaa tgcaaaattg tcccaaagat gggttccctg taccagcaaa cattatttgg 4621 cattttaaag aagatggaac tatttttcac ccacactcag gactgtgtct tagtgcttat 4681 cggacaccgg agggccgacc tgatgtacaa atgagaactt gtgatgctct agataaaaat 4741 caaatttgga gttttgagaa aTAGGACCCA GCTTTCTTGT ACAAAGTGGT TGATAGCTTG 4801 GATCCAATCA ACCTCTGGAT TACAAAATTT GTGAAAGATT GACTGGTATT CTTAACTATG 4861 TTGCTCCTTT TACGCTATGT GGATACGCTG CTTTAATGCC TTTGTATCAT GCTATTGCTT 4921 CCCGTATGGC TTTCATTTTC TCCTCCTTGT ATAAATCCTG GTTGCTGTCT CTTTATGAGG 4981 AGTTGTGGCC CGTTGTCAGG CAACGTGGCG TGGTGTGCAC TGTGTTTGCT GACGCAACCC 5041 CCACTGGTTG GGGCATTGCC ACCACCTGTC AGCTCCTTTC CGGGACTTTC GCTTTCCCCC 5101 TCCCTATTGC CACGGCGGAA CTCATCGCCG CCTGCCTTGC CCGCTGCTGG ACAGGGGCTC 5161 GGCTGTTGGG CACTGACAAT TCCGTGGTGT TGTCGGGGAA GCTGACGTCC TTTCCATGGC 5221 TGCTCGCCTG TGTTGCCACC TGGATTCTGC GCGGGACGTC CTTCTGCTAC GTCCCTTCGG 5281 CCCTCAATCC AGCGGACCTT CCTTCCCGCG GCCTGCTGCC GGCTCTGCGG CCTCTTCCGC 5341 GTCTTCGAGA TCTGCCTCGA CTGTGCCTTC TAGTTGCCAG CCATCTGTTG TTTGCCCCTC 5401 CCCCGTGCCT TCCTTGACCC TGGAAGGTGC CACTCCCACT GTCCTTTCCT AATAAAATGA 5461 GGAAATTGCA TCGCATTGTC TGAGTAGGTG TCATTCTATT CTGGGGGGTG GGGTGGGGCA 5521 GGACAGCAAG GGGGAGGATT GGGAAGACAA TAGCAGGCAT GCTGGGGACT CGAGTTAAGG 5581 GCGAATTGCC GATTAGGATC TTCCTAGAGC ATGGCTACGT AGATAAGTAG CATGGCGGGT 5641 TAATCATTAA CTACAAGGAA CCCCTAGTGA TGGAGTTGGC CACTCCCTCT CTGCGCGCTC 5701 GCTCGCTCAC TGAGGCCGGG CGACCAAAGG TCGCCCGACG CCCGGGCTTT GCCCGGGCGG 5761 CCTCAGTGAG CGAGCGAGCG CGCAGCCTTA ATTAAGAGCT CATGGCGCGC CTAGGCCTTG 5821 ACGGCCTTCC GCCAATTCGC CCTATAGTGA GTCGTATTAC GTCGCGCTCA CTGGCCGTCG 5881 TTTTACAACG TCGTGACTGG GAAAACCCTG GCGTTACCCA ACTTAATCGC CTTGCAGCAC 5941 ATCCCCCTTT CGCCAGCTGG CGTAATAGCG AAGAGGCCCG CACCGAAACG CCCTTCCCAA 6001 CAGTTGCGCA GCCTGAATGG CGAATGGGAG CGCCCTGTAG CGGCCACTCA ACCCTATCTC 6061 GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA 6121 GCTGATTTAA CAAAAATTTA ACGCGAATTT TAACAAAATA TTAACGCTTA CAATTTAG //