LOCUS GALNT5-pGEn1-DES 7252 bp DNA circular 22-JAN-2013 FEATURES Location/Qualifiers CDS 129..1108 /dnas_title="AmpR" /label=AmpR /vntifkey="4" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_feature 2977..3054 /note="TCM Sig Seq" misc_recomb complement(3114..3142) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_feature 3055..3078 /note="8xHis Tag" misc_feature 3085..3108 /note="StrepTag" misc_feature 5880..6421 /note="WPRE" misc_recomb 5842..5864 /label=attB2 /note="attB2 site" /note="recognized as attB2" misc_feature complement(6428..6642) /note="BGH pA" misc_feature complement(6730..6859) /note="3'-ITR" misc_feature 3143..3165 /note="TEV site" source 3166..5835 /dnas_title="GALNT5-pGEn1-DEST" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:164948 IMAGE:40148207" /tissue_type="Donated clones,Novartis FGA collection" /clone_lib="NIH_MGC_417" /lab_host="DH5a" /note="Vector: pCMV-SPORT6" gene 3166..5835 /dnas_title="GALNT5" /gene="GALNT5" /gene_synonym="GALNAC-T5" /db_xref="GeneID:11227" /db_xref="HGNC:4127" CDS 3166..5835 /dnas_title="UDP-N-acetyl-alpha-D-galactosamine:polypeptid e N-acetylgalactosaminyltransferase 5 (GalNAc-T5)" /gene="GALNT5" /gene_synonym="GALNAC-T5" /codon_start=1 /product="UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 5 (GalNAc-T5)" /protein_id="AAI42677.1" /db_xref="GI:148745655" /db_xref="GeneID:11227" /db_xref="HGNC:4127" /translation="MNRIRKFFRGSGRVLAFIFVASVIWLLFDMAALRLSFSEINTRV IKEDIVRRERIGFRVQPDQGKIFYSSIKEMKPPLRGHGKGAWGKENVRKTEESVLKVE VDLDQTQRERKMQNALGRGKVVPLWHPAHLQTLPVTPNKQKTDGRGTKPEASSHQGTP KQTTAQGAPKTSFIAAKGTQVVKISVHMGRVSLKQEPRKSHSPSSDTSKLAAERDLNV TISLSTDRPKQRSQAVANERAHPASTAVPKSGEAMALNKTKTQSKEVNANKHKANTSL PFPKFTVNSNRLRKQSINETPLGSLSKDDGARGAHGKKLNFSESHLVIITKEEEQKAD PKEVSNSKTKTIFPKVLGKSQSKHISRNRSEMSSSSLAPHRVPLSQTNHALTGGLEPA KINITAKAPSTEYNQSHIKALLPEDSGTHQVLRIDVTLSPRDPKAPGQFGRPVVVPHG KEKEAERRWKEGNFNVYLSDLIPVDRAIEDTRPAGCAEQLVHNNLPTTSVIMCFVDEV WSTLLRSVHSVINRSPPHLIKEILLVDDFSTKDYLKDNLDKYMSQFPKVRILRLKERH GLIRARLAGAQNATGDVLTFLDSHVECNVGWLEPLLERVYLSRKKVACPVIEVINDKD MSYMTVDNFQRGIFVWPMNFGWRTIPPDVIAKNRIKETDTIRCPVMAGGLFSIDKSYF FELGTYDPGLDVWGGENMELSFKVWMCGGEIEIIPCSRVGHIFRNDNPYSFPKDRMKT VERNLVRVAEVWLDEYKELFYGHGDHLIDQGLDVGNLTQQRELRKKLKCKSFKWYLEN VFPDLRAPIVRASGVLINVALGKCISIENTTVILEDCDGSKELQQFNYTWLRLIKCGE WCIAPIPDKGAVRLHPCDNRNKGLKWLHKSTSVFHPELVNHIVFENNQQLLCLEGNFS QKILKVAACDPVKPYQKWKFEKYYEA" misc_feature 3166..5835 /note="GALNT5 coding region" misc_difference 5304..5304 /dnas_title="GALNT5" /gene="GALNT5" /gene_synonym="GALNAC-T5" /note="'T' in cDNA is 'C' in the human genome; no amino acid change. The chimpanzee genome agrees with the cDNA sequence, suggesting that this difference is unlikely to be due to an artifact." vector join(5850..7252,1..3132) /source="pGEn1-DEST" /type="Destination Vector" clone 3133..5849 /source="GALNT5-Nterm-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCGCCATGA GACTGCTGAC AGCCCTGTTC 3001 GCCTACTTCA TCGTGGCCCT GATCCTGGCC TTCAGCGTGT CCGCCAAGAG CATGCACCAC 3061 CACCATCACC ATCATCACAG CGCCTGGTCC CACCCCCAGT TCGAGAAAGA ATTATCAACA 3121 AGTTTGTACA AAAAAGCAGG CTctgaaaac ttgtactttc aaggcaggag ggagcggata 3181 ggattcagag ttcagccaga ccaaggaaaa attttttaca gcagcataaa agagatgaaa 3241 cctcccctaa ggggacatgg gaaaggggca tggggcaaag agaatgttag aaaaactgag 3301 gagagtgtgc tcaaggttga ggtggacttg gaccaaaccc agagggaaag aaaaatgcag 3361 aatgccctgg gaaggggcaa ggttgtgccg ttgtggcatc ctgcacatct gcagaccctc 3421 cctgtgactc ctaacaagca gaagacagac gggagaggca ccaaacctga agcctcctct 3481 caccagggga caccaaagca aacgacagct cagggggctc caaagacctc attcatagca 3541 gcaaaaggaa ctcaggtagt caaaatatca gtacacatgg gacgtgtcag tttaaaacag 3601 gagccccgga agagtcatag tcccagcagt gacacatcaa aactagcagc tgaaagggac 3661 ttgaatgtga ccatcagtct tagtactgat agaccaaagc agcgatcaca ggcagtagca 3721 aacgagaggg cacaccctgc cagcacagca gtgccgaagt ctggggaagc catggcctta 3781 aacaaaacta agactcagag caaagaagtc aatgcaaata aacacaaagc caatacgagt 3841 cttccttttc ctaagttcac tgtcaattca aatcgcttaa ggaagcaatc tattaatgag 3901 acacctttgg gaagtttgtc aaaggatgat ggagctagag gggctcatgg gaagaaactc 3961 aatttctctg aaagccatct tgtgattata accaaagagg aagagcaaaa ggcagacccc 4021 aaagaggtct ctaattctaa aaccaaaaca atatttccta aagtattggg taaaagccaa 4081 agtaaacaca tttccaggaa tagaagtgag atgtcttcct cttcacttgc tccacataga 4141 gtgccactgt cccaaactaa ccatgcttta actggagggc tagagccagc aaaaatcaac 4201 ataactgcca aagccccctc tacagaatac aaccagagtc atataaaagc ccttttacct 4261 gaagacagtg gaacgcacca ggtgttaaga attgatgtga cactttctcc aagggacccc 4321 aaagctccag ggcagtttgg gcgtcctgta gttgtccccc atggaaagga gaaggaggca 4381 gaaagaagat ggaaagaagg aaacttcaat gtctacctta gcgatttgat cccagtggat 4441 agagccattg aagacaccag acctgctgga tgtgcagagc agctagttca caataacctc 4501 ccaaccacca gtgtcatcat gtgctttgtg gatgaagtgt ggtccactct cctgagatct 4561 gttcacagtg tcatcaatcg ctctcctcca cacctcatca aggagattct gctggtagat 4621 gacttcagca ccaaagacta tctaaaagat aatttggata aatacatgtc ccagtttcca 4681 aaagttcgga ttcttcgcct caaagagaga catggcttaa taagggccag gctggcagga 4741 gcacagaatg caacaggtga tgtgttgaca tttttagatt ctcatgtgga atgtaacgtt 4801 ggttggttgg aacctcttct ggaaagagtt tatttaagta gaaagaaagt ggcctgtcca 4861 gtaatcgaag tcatcaatga taaggatatg agttacatga cagtggataa ctttcaaaga 4921 ggcatctttg tgtggcccat gaactttggt tggagaacaa ttcctccaga tgtcattgca 4981 aaaaacagaa ttaaagaaac tgatacaata aggtgccctg tcatggctgg tggattgttt 5041 tctattgaca aaagttactt ttttgaactt ggaacatacg accctggcct tgatgtttgg 5101 ggtggggaaa atatggagct ctcattcaag gtgtggatgt gtggtggtga aattgagatc 5161 attccctgct cccgagtggg ccatatattc agaaatgaca atccatattc cttccccaaa 5221 gaccggatga agacagtgga gcggaacttg gtgcgggttg ccgaggtctg gctggatgag 5281 tataaggagc tgttctatgg ccatggagac cacctcatcg accaagggct agatgttggc 5341 aacctcaccc agcaaaggga gctgcgaaag aaactgaagt gcaaaagttt caaatggtac 5401 ttggagaatg tctttcctga cttaagggct cccattgtga gagctagtgg tgtgcttatt 5461 aatgtggctt tgggtaaatg catttccatt gaaaacacta cagtcattct ggaagactgc 5521 gatgggagca aagagcttca acaatttaat tacacctggt taagacttat taaatgtgga 5581 gaatggtgta tagcccccat ccctgataaa ggagccgtaa ggctgcaccc ttgtgataac 5641 agaaacaaag ggctaaaatg gctgcataaa tcaacatcag tctttcatcc agaactggtg 5701 aatcacattg tttttgaaaa caatcagcaa ttattatgct tggaaggaaa tttttctcaa 5761 aagatcctga aagtagctgc ctgtgaccca gtgaagccat atcaaaagtg gaaatttgaa 5821 aaatattatg aagccTAGGA CCCAGCTTTC TTGTACAAAG TGGTTGATAG CTTGGATCCA 5881 ATCAACCTCT GGATTACAAA ATTTGTGAAA GATTGACTGG TATTCTTAAC TATGTTGCTC 5941 CTTTTACGCT ATGTGGATAC GCTGCTTTAA TGCCTTTGTA TCATGCTATT GCTTCCCGTA 6001 TGGCTTTCAT TTTCTCCTCC TTGTATAAAT CCTGGTTGCT GTCTCTTTAT GAGGAGTTGT 6061 GGCCCGTTGT CAGGCAACGT GGCGTGGTGT GCACTGTGTT TGCTGACGCA ACCCCCACTG 6121 GTTGGGGCAT TGCCACCACC TGTCAGCTCC TTTCCGGGAC TTTCGCTTTC CCCCTCCCTA 6181 TTGCCACGGC GGAACTCATC GCCGCCTGCC TTGCCCGCTG CTGGACAGGG GCTCGGCTGT 6241 TGGGCACTGA CAATTCCGTG GTGTTGTCGG GGAAGCTGAC GTCCTTTCCA TGGCTGCTCG 6301 CCTGTGTTGC CACCTGGATT CTGCGCGGGA CGTCCTTCTG CTACGTCCCT TCGGCCCTCA 6361 ATCCAGCGGA CCTTCCTTCC CGCGGCCTGC TGCCGGCTCT GCGGCCTCTT CCGCGTCTTC 6421 GAGATCTGCC TCGACTGTGC CTTCTAGTTG CCAGCCATCT GTTGTTTGCC CCTCCCCCGT 6481 GCCTTCCTTG ACCCTGGAAG GTGCCACTCC CACTGTCCTT TCCTAATAAA ATGAGGAAAT 6541 TGCATCGCAT TGTCTGAGTA GGTGTCATTC TATTCTGGGG GGTGGGGTGG GGCAGGACAG 6601 CAAGGGGGAG GATTGGGAAG ACAATAGCAG GCATGCTGGG GACTCGAGTT AAGGGCGAAT 6661 TGCCGATTAG GATCTTCCTA GAGCATGGCT ACGTAGATAA GTAGCATGGC GGGTTAATCA 6721 TTAACTACAA GGAACCCCTA GTGATGGAGT TGGCCACTCC CTCTCTGCGC GCTCGCTCGC 6781 TCACTGAGGC CGGGCGACCA AAGGTCGCCC GACGCCCGGG CTTTGCCCGG GCGGCCTCAG 6841 TGAGCGAGCG AGCGCGCAGC CTTAATTAAG AGCTCATGGC GCGCCTAGGC CTTGACGGCC 6901 TTCCGCCAAT TCGCCCTATA GTGAGTCGTA TTACGTCGCG CTCACTGGCC GTCGTTTTAC 6961 AACGTCGTGA CTGGGAAAAC CCTGGCGTTA CCCAACTTAA TCGCCTTGCA GCACATCCCC 7021 CTTTCGCCAG CTGGCGTAAT AGCGAAGAGG CCCGCACCGA AACGCCCTTC CCAACAGTTG 7081 CGCAGCCTGA ATGGCGAATG GGAGCGCCCT GTAGCGGCCA CTCAACCCTA TCTCGGTCTA 7141 TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT 7201 TTAACAAAAA TTTAACGCGA ATTTTAACAA AATATTAACG CTTACAATTT AG //