LOCUS GALNT6-pGEn1-DES 6349 bp DNA circular 22-JAN-2013 FEATURES Location/Qualifiers CDS 129..1108 /dnas_title="AmpR" /label=AmpR /vntifkey="4" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_feature 2977..3054 /note="TCM Sig Seq" misc_recomb complement(3114..3142) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_feature 3055..3078 /note="8xHis Tag" misc_feature 3085..3108 /note="StrepTag" misc_feature 4977..5518 /note="WPRE" misc_recomb 4939..4961 /label=attB2 /note="attB2 site" /note="recognized as attB2" misc_feature complement(5525..5739) /note="BGH pA" misc_feature complement(5827..5956) /note="3'-ITR" misc_feature 3143..3165 /note="TEV site" source 3166..4932 /dnas_title="GALNT6-pGEn1-DEST" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:46032 IMAGE:5552059" /tissue_type="Lymph, lymphoma" /clone_lib="NIH_MGC_85" /lab_host="DH10B" /note="Vector: pCMV-SPORT6" gene 3166..4932 /dnas_title="GALNT6" /gene="GALNT6" /gene_synonym="GALNAC-T6" /gene_synonym="GalNAcT6" /db_xref="GeneID:11226" /db_xref="HGNC:4128" /db_xref="MIM:605148" CDS 3166..4932 /dnas_title="UDP-N-acetyl-alpha-D-galactosamine:polypeptid e N-acetylgalactosaminyltransferase 6 (GalNAc-T6)" /gene="GALNT6" /gene_synonym="GALNAC-T6" /gene_synonym="GalNAcT6" /codon_start=1 /product="UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 6 (GalNAc-T6)" /protein_id="AAH35822.2" /db_xref="GI:37572269" /db_xref="GeneID:11226" /db_xref="HGNC:4128" /db_xref="MIM:605148" /translation="MRLLRRRHMPLRLAMVGCAFVLFLFLLHRDVSSREEATEKPWLK SLVSRKDHVLDLMLEAMNNLRDSMPKLQIRAPEAQQTLFSINQSCLPGFYTPAELKPF WERPPQDPNAPGADGKAFQKSKWTPLETQEKEEGYKKHCFNAFASDRISLQRSLGPDT RPPECVDQKFRRCPPLATTSVIIVFHNEAWSTLLRTVYSVLHTTPAILLKEIILVDDA STEEHLKEKLEQYVKQLQVVRVVRQEERKGLITARLLGASVAQAEVLTFLDAHCECFH GWLEPLLARIAEDKTVVVSPDIVTIDLNTFEFAKPVQRGRVHSRGNFDWSLTFGWETL PPHEKQRRKDETYPIKSPTFAGGLFSISKSYFEHIGTYDNQMEIWGGENVEMSFRVWQ CGGQLEIIPCSVVGHVFRTKSPHTFPKGTSVIARNQVRLAEVWMDSYKKIFYRRNLQA AKMAQEKSFGDISERLQLREQLHCHNFSWYLHNVYPEMFVPDLTPTFYGAIKNLGTNQ CLDVGENNRGGKPLIMYSCHGLGGNQYFEYTTQRDLRHNIAKQLCLHVSKGALGLGSC HFTGKNSQVPKDEEWELAQDQLIRNSGSGTCLTSQDKKPAMAPCNPSDPHQLWLFV" misc_feature 3166..4932 /note="GALNT6 coding region" vector join(4947..6349,1..3132) /source="pGEn1-DEST" /type="Destination Vector" clone 3133..4946 /source="GALNT6-Nterm-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCGCCATGA GACTGCTGAC AGCCCTGTTC 3001 GCCTACTTCA TCGTGGCCCT GATCCTGGCC TTCAGCGTGT CCGCCAAGAG CATGCACCAC 3061 CACCATCACC ATCATCACAG CGCCTGGTCC CACCCCCAGT TCGAGAAAGA ATTATCAACA 3121 AGTTTGTACA AAAAAGCAGG CTctgaaaac ttgtactttc aaggcagaga ggaggccaca 3181 gagaagccgt ggctgaagtc cctggtgagc cggaaggatc acgtcctgga cctcatgctg 3241 gaggccatga acaaccttag agattcaatg cccaagctcc aaatcagggc tccagaagcc 3301 cagcagactc tgttctccat aaaccagtcc tgcctccctg ggttctatac cccagctgaa 3361 ctgaagccct tctgggaacg gccaccacag gaccccaatg cccctggggc agatggaaaa 3421 gcatttcaga agagcaagtg gacccccctg gagacccagg aaaaggaaga aggctataag 3481 aagcactgtt tcaatgcctt tgccagcgac cggatctccc tgcagaggtc cctggggcca 3541 gacacccgac cacctgagtg tgtggaccag aagttccggc gctgcccccc actggccacc 3601 accagcgtga tcattgtgtt ccacaacgaa gcctggtcca cactgctgcg aacagtgtac 3661 agcgtcctac acaccacccc tgccatcttg ctcaaggaga tcatactggt ggatgatgcc 3721 agcacagagg agcacctaaa ggagaagctg gagcagtacg tgaagcagct gcaggtggtg 3781 agggtggtgc ggcaggagga gcggaagggg ctgatcaccg cccggctgct gggggccagc 3841 gtggcacagg cggaggtgct cacgttcctg gatgcccact gtgagtgctt ccacggctgg 3901 ctggagcccc tcctggctcg aatcgctgag gacaagacag tggtggtgag cccagacatc 3961 gtcaccatcg accttaatac ttttgagttc gccaagcccg tccagagggg cagagtccat 4021 agccgaggca actttgactg gagcctgacc ttcggctggg aaacacttcc tccacatgag 4081 aagcagaggc gcaaggatga aacctacccc atcaaatccc cgacgtttgc tggtggcctc 4141 ttctccatct ccaagtccta ctttgagcac atcggtacct atgataatca gatggagatc 4201 tggggagggg agaacgtgga aatgtccttc cgggtgtggc agtgtggggg ccagctggag 4261 atcatcccct gctctgtcgt aggccatgtg ttccggacca agagccccca caccttcccc 4321 aagggcacta gtgtcattgc tcgcaatcaa gtgcgcctgg cagaggtctg gatggacagc 4381 tacaagaaga ttttctatag gagaaatctg caggcagcaa agatggccca agagaaatcc 4441 ttcggtgaca tttcggaacg actgcagctg agggaacaac tgcactgtca caacttttcc 4501 tggtacctgc acaatgtcta cccagagatg tttgttcctg acctgacgcc caccttctat 4561 ggtgccatca agaacctcgg caccaaccaa tgcctggatg tgggtgagaa caaccgcggg 4621 gggaagcccc tcatcatgta ctcctgccac ggccttggcg gcaaccagta ctttgagtac 4681 acaactcaga gggaccttcg ccacaacatc gcaaagcagc tgtgtctaca tgtcagcaag 4741 ggtgctctgg gccttgggag ctgtcacttc actggcaaga atagccaggt ccccaaggac 4801 gaggaatggg aattggccca ggatcagctc atcaggaact caggatctgg tacctgcctg 4861 acatcccagg acaaaaagcc agccatggcc ccctgcaatc ccagtgaccc ccatcagttg 4921 tggctctttg tcTAGGACCC AGCTTTCTTG TACAAAGTGG TTGATAGCTT GGATCCAATC 4981 AACCTCTGGA TTACAAAATT TGTGAAAGAT TGACTGGTAT TCTTAACTAT GTTGCTCCTT 5041 TTACGCTATG TGGATACGCT GCTTTAATGC CTTTGTATCA TGCTATTGCT TCCCGTATGG 5101 CTTTCATTTT CTCCTCCTTG TATAAATCCT GGTTGCTGTC TCTTTATGAG GAGTTGTGGC 5161 CCGTTGTCAG GCAACGTGGC GTGGTGTGCA CTGTGTTTGC TGACGCAACC CCCACTGGTT 5221 GGGGCATTGC CACCACCTGT CAGCTCCTTT CCGGGACTTT CGCTTTCCCC CTCCCTATTG 5281 CCACGGCGGA ACTCATCGCC GCCTGCCTTG CCCGCTGCTG GACAGGGGCT CGGCTGTTGG 5341 GCACTGACAA TTCCGTGGTG TTGTCGGGGA AGCTGACGTC CTTTCCATGG CTGCTCGCCT 5401 GTGTTGCCAC CTGGATTCTG CGCGGGACGT CCTTCTGCTA CGTCCCTTCG GCCCTCAATC 5461 CAGCGGACCT TCCTTCCCGC GGCCTGCTGC CGGCTCTGCG GCCTCTTCCG CGTCTTCGAG 5521 ATCTGCCTCG ACTGTGCCTT CTAGTTGCCA GCCATCTGTT GTTTGCCCCT CCCCCGTGCC 5581 TTCCTTGACC CTGGAAGGTG CCACTCCCAC TGTCCTTTCC TAATAAAATG AGGAAATTGC 5641 ATCGCATTGT CTGAGTAGGT GTCATTCTAT TCTGGGGGGT GGGGTGGGGC AGGACAGCAA 5701 GGGGGAGGAT TGGGAAGACA ATAGCAGGCA TGCTGGGGAC TCGAGTTAAG GGCGAATTGC 5761 CGATTAGGAT CTTCCTAGAG CATGGCTACG TAGATAAGTA GCATGGCGGG TTAATCATTA 5821 ACTACAAGGA ACCCCTAGTG ATGGAGTTGG CCACTCCCTC TCTGCGCGCT CGCTCGCTCA 5881 CTGAGGCCGG GCGACCAAAG GTCGCCCGAC GCCCGGGCTT TGCCCGGGCG GCCTCAGTGA 5941 GCGAGCGAGC GCGCAGCCTT AATTAAGAGC TCATGGCGCG CCTAGGCCTT GACGGCCTTC 6001 CGCCAATTCG CCCTATAGTG AGTCGTATTA CGTCGCGCTC ACTGGCCGTC GTTTTACAAC 6061 GTCGTGACTG GGAAAACCCT GGCGTTACCC AACTTAATCG CCTTGCAGCA CATCCCCCTT 6121 TCGCCAGCTG GCGTAATAGC GAAGAGGCCC GCACCGAAAC GCCCTTCCCA ACAGTTGCGC 6181 AGCCTGAATG GCGAATGGGA GCGCCCTGTA GCGGCCACTC AACCCTATCT CGGTCTATTC 6241 TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA 6301 ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGCTT ACAATTTAG //