LOCUS GALNT7-pGEn1-DES 6436 bp DNA circular 22-JAN-2013 FEATURES Location/Qualifiers CDS 129..1108 /dnas_title="AmpR" /label=AmpR /vntifkey="4" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_feature 2977..3054 /note="TCM Sig Seq" misc_recomb complement(3114..3142) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_feature 3055..3078 /note="8xHis Tag" misc_feature 3085..3108 /note="StrepTag" misc_feature 5064..5605 /note="WPRE" misc_recomb 5026..5048 /label=attB2 /note="attB2 site" /note="recognized as attB2" misc_feature complement(5612..5826) /note="BGH pA" misc_feature complement(5914..6043) /note="3'-ITR" misc_feature 3143..3165 /note="TEV site" source 3166..5019 /dnas_title="GALNT7-pGEn1-DEST" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:57606 IMAGE:4442080" /tissue_type="Liver, adenocarcinoma" /clone_lib="NIH_MGC_90" /lab_host="DH10B" /note="Vector: pCMV-SPORT6" gene 3166..5019 /dnas_title="GALNT7" /gene="GALNT7" /gene_synonym="GALNAC-T7" /gene_synonym="GalNAcT7" /db_xref="GeneID:51809" /db_xref="HGNC:4129" /db_xref="MIM:605005" CDS 3166..5019 /dnas_title="UDP-N-acetyl-alpha-D-galactosamine:polypeptid e N-acetylgalactosaminyltransferase 7 (GalNAc-T7)" /gene="GALNT7" /gene_synonym="GALNAC-T7" /gene_synonym="GalNAcT7" /codon_start=1 /product="UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 7 (GalNAc-T7)" /protein_id="AAH46129.1" /db_xref="GI:28279289" /db_xref="GeneID:51809" /db_xref="HGNC:4129" /db_xref="MIM:605005" /translation="MRLKIGFILRSLLVVGSFLGLVVLWSSLTPRPDDPSPLSRMRED RDVNDPMPNRGGNGLAPGEDRFKPVVPWPHVEGVEVDLESIRRINKAKNEQEHHAGGD SQKDIMQRQYLTFKPQTFTYHDPVLRPGILGNFEPKEPEPPGVVGGPGEKAKPLVLGP EFKQAIQASIKEFGFNMVASDMISLDRSVNDLRQEECKYWHYDENLLTSSVVIVFHNE GWSTLMRTVHSVIKRTPRKYLAEIVLIDDFSNKEHLKEKLDEYIKLWNGLVKVFRNER REGLIQARSIGAQKAKLGQVLIYLDAHCEVAVNWYAPLVAPISKDRTICTVPLIDVIN GNTYEIIPQGGGDEDGYARGAWDWSMLWKRVPLTPQEKRLRKTKTEPYRSPAMAGGLF AIEREFFFELGLYDPGLQIWGGENFEISYKIWQCGGKLLFVPCSRVGHIYRLEGWQGN PPPIYVGSSPTLKNYVRVVEVWWDEYKDYFYASRPESQALPYGDISELKKFREDHNCK SFKWFMEEIAYDITSHYPLPPKNVDWGEIRGFETAYCIDSMGKTNGGFVELGPCHRMG GNQLFRINEANQLMQYDQCLTKGADGSKVMITHCNLNEFKEWQYFKNLHRFTHIPSGK CLDRSEVLHQVFISNCDSSKTTQKWEMNNIHSV" misc_feature 3166..5019 /note="GALNT7 coding region" vector join(5034..6436,1..3132) /source="pGEn1-DEST" /type="Destination Vector" clone 3133..5033 /source="GALNT7-Nterm-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCGCCATGA GACTGCTGAC AGCCCTGTTC 3001 GCCTACTTCA TCGTGGCCCT GATCCTGGCC TTCAGCGTGT CCGCCAAGAG CATGCACCAC 3061 CACCATCACC ATCATCACAG CGCCTGGTCC CACCCCCAGT TCGAGAAAGA ATTATCAACA 3121 AGTTTGTACA AAAAAGCAGG CTctgaaaac ttgtactttc aaggcaggat gagggaagac 3181 agagatgtca atgaccccat gcccaaccga ggcggcaatg gactagctcc tggggaggac 3241 agattcaaac ctgtggtacc atggcctcat gttgaaggag tagaagtgga cttagagtct 3301 attagaagaa taaacaaggc caaaaatgaa caagagcacc atgctggagg agattcccag 3361 aaagatatca tgcagaggca gtatctcaca tttaagcctc agacattcac ctaccatgat 3421 cctgtgcttc gcccagggat cctcggtaac tttgaaccca aagaacctga gcctcctgga 3481 gtggttggtg gccctggaga gaaagccaag ccattggttt tgggaccaga attcaaacaa 3541 gcaattcaag ccagcattaa agagtttgga tttaacatgg tggcaagtga catgatctca 3601 ctggaccgca gcgtcaatga cttacgccaa gaagaatgca agtattggca ttatgatgaa 3661 aacttgctca cttcgagcgt tgtcattgtc ttccataatg aaggatggtc aaccctcatg 3721 agaacagtcc acagtgtaat taaaaggact ccaaggaaat atttagcaga aattgtgtta 3781 attgacgatt tcagtaataa agaacactta aaagaaaaac tggatgaata tattaagctg 3841 tggaatggcc tagtgaaggt atttcgaaat gaaagaaggg aaggtttaat tcaagcacga 3901 agtattggtg ctcagaaggc taaacttgga caggttttga tataccttga tgcccactgt 3961 gaggtggcag ttaactggta tgcaccactt gtagctccca tatctaagga cagaaccatt 4021 tgcactgtgc cgcttataga tgtcataaat ggcaacacat atgaaattat accccaaggg 4081 ggtggtgatg aagatgggta tgcccgagga gcatgggatt ggagtatgct ctggaaacgg 4141 gtgcctctga cccctcaaga gaagagactg agaaagacaa aaactgaacc gtatcggtcc 4201 ccagccatgg ctgggggatt atttgccatt gaacgagagt tcttctttga attgggtctc 4261 tatgatccag gtctccagat ttggggtggt gaaaactttg agatctcata caagatatgg 4321 cagtgtggtg gcaaattatt atttgttcct tgttctcgtg ttggacatat ctaccgtctt 4381 gagggctggc aaggaaatcc tccgcccatt tatgttgggt cttctccaac tctgaagaat 4441 tatgttagag ttgtggaggt ttggtgggat gaatataaag actacttcta tgctagtcgt 4501 cctgaatcgc aggcattacc atatggggat atatcggagc tgaaaaaatt tcgagaagat 4561 cacaactgca aaagttttaa gtggttcatg gaagaaatag cttatgatat cacctcacac 4621 taccctttgc cacccaaaaa tgttgactgg ggagaaatca gaggcttcga aactgcttac 4681 tgcattgata gcatgggaaa aacaaatgga ggctttgttg aactaggacc ctgccacagg 4741 atgggaggga atcagctttt cagaatcaat gaagcaaatc aactcatgca gtatgaccag 4801 tgtttgacaa agggagctga tggatcaaaa gttatgatta cacactgtaa tctaaatgaa 4861 tttaaggaat ggcagtactt caagaacctg cacagattta ctcatattcc ttcaggaaag 4921 tgtttagatc gctcagaggt cctgcatcaa gtattcatct ccaattgtga ctccagtaaa 4981 acgactcaaa aatgggaaat gaataacatc catagtgttT AGGACCCAGC TTTCTTGTAC 5041 AAAGTGGTTG ATAGCTTGGA TCCAATCAAC CTCTGGATTA CAAAATTTGT GAAAGATTGA 5101 CTGGTATTCT TAACTATGTT GCTCCTTTTA CGCTATGTGG ATACGCTGCT TTAATGCCTT 5161 TGTATCATGC TATTGCTTCC CGTATGGCTT TCATTTTCTC CTCCTTGTAT AAATCCTGGT 5221 TGCTGTCTCT TTATGAGGAG TTGTGGCCCG TTGTCAGGCA ACGTGGCGTG GTGTGCACTG 5281 TGTTTGCTGA CGCAACCCCC ACTGGTTGGG GCATTGCCAC CACCTGTCAG CTCCTTTCCG 5341 GGACTTTCGC TTTCCCCCTC CCTATTGCCA CGGCGGAACT CATCGCCGCC TGCCTTGCCC 5401 GCTGCTGGAC AGGGGCTCGG CTGTTGGGCA CTGACAATTC CGTGGTGTTG TCGGGGAAGC 5461 TGACGTCCTT TCCATGGCTG CTCGCCTGTG TTGCCACCTG GATTCTGCGC GGGACGTCCT 5521 TCTGCTACGT CCCTTCGGCC CTCAATCCAG CGGACCTTCC TTCCCGCGGC CTGCTGCCGG 5581 CTCTGCGGCC TCTTCCGCGT CTTCGAGATC TGCCTCGACT GTGCCTTCTA GTTGCCAGCC 5641 ATCTGTTGTT TGCCCCTCCC CCGTGCCTTC CTTGACCCTG GAAGGTGCCA CTCCCACTGT 5701 CCTTTCCTAA TAAAATGAGG AAATTGCATC GCATTGTCTG AGTAGGTGTC ATTCTATTCT 5761 GGGGGGTGGG GTGGGGCAGG ACAGCAAGGG GGAGGATTGG GAAGACAATA GCAGGCATGC 5821 TGGGGACTCG AGTTAAGGGC GAATTGCCGA TTAGGATCTT CCTAGAGCAT GGCTACGTAG 5881 ATAAGTAGCA TGGCGGGTTA ATCATTAACT ACAAGGAACC CCTAGTGATG GAGTTGGCCA 5941 CTCCCTCTCT GCGCGCTCGC TCGCTCACTG AGGCCGGGCG ACCAAAGGTC GCCCGACGCC 6001 CGGGCTTTGC CCGGGCGGCC TCAGTGAGCG AGCGAGCGCG CAGCCTTAAT TAAGAGCTCA 6061 TGGCGCGCCT AGGCCTTGAC GGCCTTCCGC CAATTCGCCC TATAGTGAGT CGTATTACGT 6121 CGCGCTCACT GGCCGTCGTT TTACAACGTC GTGACTGGGA AAACCCTGGC GTTACCCAAC 6181 TTAATCGCCT TGCAGCACAT CCCCCTTTCG CCAGCTGGCG TAATAGCGAA GAGGCCCGCA 6241 CCGAAACGCC CTTCCCAACA GTTGCGCAGC CTGAATGGCG AATGGGAGCG CCCTGTAGCG 6301 GCCACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC 6361 CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTTA ACAAAATATT 6421 AACGCTTACA ATTTAG //