LOCUS GALNTL2-pGEn3-DE 7761 bp DNA circular 22-JAN-2013 FEATURES Location/Qualifiers CDS 129..1108 /dnas_title="AmpR" /label=AmpR /vntifkey="4" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_recomb complement(4541..4569) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_feature 6389..6930 /note="WPRE" misc_recomb 6351..6373 /label=attB2 /note="attB2 site" /note="recognized as attB2" misc_feature complement(6937..7151) /note="BGH pA" misc_feature complement(7239..7368) /note="3'-ITR" misc_feature 2982..3056 /note="TCM signal sequence" misc_feature 3060..3083 /note="His tag" misc_feature 3084..3134 /note="AviTag" misc_feature 3135..3830 /note="Superfolder GFP" misc_feature 3837..4532 /note="Fc domain" misc_feature 2976..2981 /note="Kozak seq" misc_feature 4570..4592 /note="TEV site" source 4593..6344 /dnas_title="GALNTL2-pGEn3-DEST" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:17334 IMAGE:4212702" /tissue_type="Brain, glioblastoma with EGFR amplification" /clone_lib="NCI_CGAP_Brn64" /lab_host="DH10B" /note="Vector: pCMV-SPORT6" gene 4593..6344 /dnas_title="GALNTL2" /gene="GALNTL2" /gene_synonym="GALNT13" /gene_synonym="GALNT15" /gene_synonym="GALNT7" /db_xref="GeneID:117248" /db_xref="HGNC:21531" CDS 4593..6344 /dnas_title="UDP-N-acetyl-alpha-D-galactosamine:polypeptid e N-acetylgalactosaminyltransferase-like 2" /gene="GALNTL2" /gene_synonym="GALNT13" /gene_synonym="GALNT15" /gene_synonym="GALNT7" /codon_start=1 /product="UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase-like 2" /protein_id="AAH14789.1" /db_xref="GI:15928642" /db_xref="GeneID:117248" /db_xref="HGNC:21531" /translation="MLLRKRYRHRPCRLQFLLLLLMLGCVLMMVAMLHPPHHTLHQTV TAQASKHSPEARYRLDFGESQDWVLEAEDEGEEYSPLEGLPPFISLREDQLLVAVALP QARRNQSQGRRGGSYRLIKQPRRQDKEAPKRDWGADEDGEVSEEEELTPFSLDPRGLQ EALSARIPLQRALPEVRHPLCLQQHPQDSLPTASVILCFHDEAWSTLLRTVHSILDTV PRAFLKEIILVDDLSQQGQLKSALSEYVARLEGVKLLRSNKRLGAIRARMLGATRATG DVLVFMDAHCECHPGWLEPLLSRIAGDRSRVVSPVIDVIDWKTFQYYPSKDLQRGVLD WKLDFHWEPLPEHVRKALQSPISPIRSPVVPGEVVAMDRHYFQNTGAYDSLMSLRGGE NLELSFKAWLCGGSVEILPCSRVGHIYQNQDSHSPLDQETTLRNRVRIAETWLGSFKE TFYKHSPEAFSLSKAEKPDCMERLQLQRRLGCRTFHWFLANVYPELYPSEPRPSFSGK LHNTGLGLCADCQAEGDILGCPMVLAPCSDSRQQQYLQHTSRKEIHFGSPQHLCFAVR QEQVILQNCTEEGLAIHQQHWDFQENGMIVHILSGKCMEAVVQENNKDLYLRPCDGKA RQQWRFDQINAVDER" misc_feature 4593..6344 /note="GALNTL2 coding region" misc_difference 5237..5237 /dnas_title="GALNTL2" /gene="GALNTL2" /gene_synonym="GALNT13" /gene_synonym="GALNT15" /gene_synonym="GALNT7" /note="'A' in cDNA is 'G' in the human genome; no amino acid change. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." misc_difference 5721..5721 /dnas_title="GALNTL2" /gene="GALNTL2" /gene_synonym="GALNT13" /gene_synonym="GALNT15" /gene_synonym="GALNT7" /note="'A' in cDNA is 'G' in the human genome; amino acid difference: 'T' in cDNA, 'A' in the human genome. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." vector join(6359..7761,1..4559) /source="pGEn3-DEST" /type="Destination Vector" clone 4560..6358 /source="GALNTL2-Nterm-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAgcg gccgcGCCAC cATGAGACTG CTGACCGCCC 3001 TGTTCGCCTA CTTCATCGTG GCCCTGATCC TGGCCTTCAG CGTGTCCGCC AAGAGCATGC 3061 ACCACCACCA TCACCATCAT CACATGAGCG GCCTGAACGA CATCTTCGAG GCCCAGAAAA 3121 TCGAGTGGCA CGAGATGAGC AAGGGCGAGG AACTGTTCAC CGGCGTGGTG CCCATCCTGG 3181 TGGAACTGGA CGGCGACGTG AACGGCCACA AGTTCTCTGT GCGGGGCGAG GGCGAGGGGG 3241 ACGCCACAAA TGGCAAGCTG ACCCTGAAGT TCATCTGCAC CACCGGAAAG CTGCCCGTGC 3301 CCTGGCCTAC CCTGGTCACA ACCCTGACCT ACGGCGTGCA GTGCTTCAGC AGATACCCCG 3361 ACCACATGAA GCGGCACGAT TTCTTCAAGA GCGCCATGCC CGAGGGCTAC GTGCAAGAAC 3421 GGACCATCAG CTTCAAGGAC GACGGCACCT ACAAGACCAG AGCCGAAGTG AAGTTCGAGG 3481 GCGACACCCT GGTCAACCGG ATCGAGCTGA AGGGCATCGA CTTCAAAGAG GACGGCAACA 3541 TCCTGGGCCA CAAGCTCGAG TACAACTTCA ACAGCCACAA CGTGTACATC ACCGCCGACA 3601 AGCAGAAGAA CGGCATCAAG GCCAACTTCA AGATCCGGCA CAACGTGGAA GATGGCAGCG 3661 TGCAGCTGGC CGACCACTAC CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC 3721 CCGACAATCA CTACCTGAGC ACCCAGAGCG TGCTGAGCAA GGACCCCAAC GAGAAGCGGG 3781 ACCACATGGT GCTGCTGGAA TTTGTGACCG CCGCTGGCAT CACCCACGGC GAATTCgagc 3841 ccaaatcttg tgacaaaact cacacatgcc caccgtgccc agcacctgaa ctcctggggg 3901 gaccgtcagt cttcctcttc cccccaaaac ccaaggacac cctcatgatc tcccggaccc 3961 ctgaggtcac atgcgtggtg gtggacgtga gccacgaaga ccctgaggtc aagttcaact 4021 ggtacgtgga cggcgtggag gtgcataatg ccaagacaaa gccgcgggag gagcagtaca 4081 acagcacgta ccgtgtggtc agcgtcctca ccgtcctgca ccaggactgg ctgaatggca 4141 aggagtacaa gtgcaaggtc tccaacaaag ccctcccagc ccccatcgag aaaaccatct 4201 ccaaagccaa agggcagccc cgagaaccac aggtgtacac cctgccccca tcccgggatg 4261 agctgaccaa gaaccaggtc agcctgacct gcctggtcaa aggcttctat cccagcgaca 4321 tcgccgtgga gtgggagagc aatgggcagc cggagaacaa ctacaagacc acgcctcccg 4381 tgctggactc cgacggctcc ttcttcctct acagcaagct caccgtggac aagagcaggt 4441 ggcagcaggg gaacgtcttc tcatgctccg tgatgcatga ggctctgcac aaccactaca 4501 cgcagaagag cctctccctg tctccgggta aagaattcgc ATCAACAAGT TTGTACAAAA 4561 AAGCAGGCTc tgaaaacttg tactttcaag gcaggtaccg cctggacttt ggggaatccc 4621 aggattgggt actggaagct gaggatgagg gtgaagagta cagccctctg gagggcctgc 4681 caccctttat ctcactgcgg gaggatcagc tgctggtggc cgtggcctta ccccaggcca 4741 gaaggaacca gagccagggc aggagaggtg ggagctaccg cctcatcaag cagccaagga 4801 ggcaggataa ggaagcccca aagagggact ggggggctga tgaggacggg gaggtgtctg 4861 aagaagagga gttgaccccg ttcagcctgg acccacgtgg cctccaggag gcactcagtg 4921 cccgcatccc cctccagagg gctctgcccg aggtgcggca cccactgtgt ctgcagcagc 4981 accctcagga cagcctgccc acagccagcg tcatcctctg tttccatgat gaggcctggt 5041 ccactctcct gcggactgta cacagcatcc tcgacacagt gcccagggcc ttcctgaagg 5101 agatcatcct cgtggacgac ctcagccagc aaggacaact caagtctgct ctcagcgaat 5161 atgtggccag gctggagggg gtgaagttac tcaggagcaa caagaggctg ggtgccatca 5221 gggcccggat gctgggagcc accagagcca ccggggatgt gctcgtcttc atggatgccc 5281 actgcgagtg ccacccaggc tggctggagc ccctcctcag cagaatagct ggtgacagga 5341 gccgagtggt atctccggtg atagatgtga ttgactggaa gactttccag tattacccct 5401 caaaggacct gcagcgtggg gtgttggact ggaagctgga tttccactgg gaacctttgc 5461 cagagcatgt gaggaaggcc ctccagtccc ccataagccc catcaggagc cctgtggtgc 5521 ccggagaggt ggtggccatg gacagacatt acttccaaaa cactggagcg tatgactctc 5581 ttatgtcgct gcgaggtggt gaaaacctcg aactgtcttt caaggcctgg ctctgtggtg 5641 gctctgttga aatccttccc tgctctcggg taggacacat ctaccaaaat caggattccc 5701 attcccccct cgaccaggag accaccctga ggaacagggt tcgcattgct gagacctggc 5761 tggggtcatt caaagaaacc ttctacaagc atagcccaga ggccttctcc ttgagcaagg 5821 ctgagaagcc agactgcatg gaacgcttgc agctgcaaag gagactgggt tgtcggacat 5881 tccactggtt tctggctaat gtctaccctg agctgtaccc atctgaaccc aggcccagtt 5941 tctctggaaa gctccacaac actggacttg ggctctgtgc agactgccag gcagaagggg 6001 acatcctggg ctgtcccatg gtgttggctc cttgcagtga cagccggcag caacagtacc 6061 tgcagcacac cagcaggaag gagattcact ttggcagccc acagcacctg tgctttgctg 6121 tcaggcagga gcaggtgatt cttcagaact gcacggagga aggcctggcc atccaccagc 6181 agcactggga cttccaggag aatgggatga ttgtccacat tctttctggg aaatgcatgg 6241 aagctgtggt gcaagaaaac aataaagatt tgtacctgcg tccgtgtgat ggaaaagccc 6301 gccagcagtg gcgttttgac cagatcaatg ctgtggatga acgaTAGGAC CCAGCTTTCT 6361 TGTACAAAGT GGTTGATAGC TTGGATCCAA TCAACCTCTG GATTACAAAA TTTGTGAAAG 6421 ATTGACTGGT ATTCTTAACT ATGTTGCTCC TTTTACGCTA TGTGGATACG CTGCTTTAAT 6481 GCCTTTGTAT CATGCTATTG CTTCCCGTAT GGCTTTCATT TTCTCCTCCT TGTATAAATC 6541 CTGGTTGCTG TCTCTTTATG AGGAGTTGTG GCCCGTTGTC AGGCAACGTG GCGTGGTGTG 6601 CACTGTGTTT GCTGACGCAA CCCCCACTGG TTGGGGCATT GCCACCACCT GTCAGCTCCT 6661 TTCCGGGACT TTCGCTTTCC CCCTCCCTAT TGCCACGGCG GAACTCATCG CCGCCTGCCT 6721 TGCCCGCTGC TGGACAGGGG CTCGGCTGTT GGGCACTGAC AATTCCGTGG TGTTGTCGGG 6781 GAAGCTGACG TCCTTTCCAT GGCTGCTCGC CTGTGTTGCC ACCTGGATTC TGCGCGGGAC 6841 GTCCTTCTGC TACGTCCCTT CGGCCCTCAA TCCAGCGGAC CTTCCTTCCC GCGGCCTGCT 6901 GCCGGCTCTG CGGCCTCTTC CGCGTCTTCG AGATCTGCCT CGACTGTGCC TTCTAGTTGC 6961 CAGCCATCTG TTGTTTGCCC CTCCCCCGTG CCTTCCTTGA CCCTGGAAGG TGCCACTCCC 7021 ACTGTCCTTT CCTAATAAAA TGAGGAAATT GCATCGCATT GTCTGAGTAG GTGTCATTCT 7081 ATTCTGGGGG GTGGGGTGGG GCAGGACAGC AAGGGGGAGG ATTGGGAAGA CAATAGCAGG 7141 CATGCTGGGG ACTCGAGTTA AGGGCGAATT GCCGATTAGG ATCTTCCTAG AGCATGGCTA 7201 CGTAGATAAG TAGCATGGCG GGTTAATCAT TAACTACAAG GAACCCCTAG TGATGGAGTT 7261 GGCCACTCCC TCTCTGCGCG CTCGCTCGCT CACTGAGGCC GGGCGACCAA AGGTCGCCCG 7321 ACGCCCGGGC TTTGCCCGGG CGGCCTCAGT GAGCGAGCGA GCGCGCAGCC TTAATTAAGA 7381 GCTCATGGCG CGCCTAGGCC TTGACGGCCT TCCGCCAATT CGCCCTATAG TGAGTCGTAT 7441 TACGTCGCGC TCACTGGCCG TCGTTTTACA ACGTCGTGAC TGGGAAAACC CTGGCGTTAC 7501 CCAACTTAAT CGCCTTGCAG CACATCCCCC TTTCGCCAGC TGGCGTAATA GCGAAGAGGC 7561 CCGCACCGAA ACGCCCTTCC CAACAGTTGC GCAGCCTGAA TGGCGAATGG GAGCGCCCTG 7621 TAGCGGCCAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 7681 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTTAACAAA 7741 ATATTAACGC TTACAATTTA G //