LOCUS GALNTL6-pGEn1-DE 6193 bp DNA circular 22-JAN-2013 FEATURES Location/Qualifiers CDS 129..1108 /dnas_title="AmpR" /label=AmpR /vntifkey="4" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_feature 2977..3054 /note="TCM Sig Seq" misc_recomb complement(3114..3142) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_feature 3055..3078 /note="8xHis Tag" misc_feature 3085..3108 /note="StrepTag" misc_feature 4821..5362 /note="WPRE" misc_recomb 4783..4805 /label=attB2 /note="attB2 site" /note="recognized as attB2" misc_feature complement(5369..5583) /note="BGH pA" misc_feature complement(5671..5800) /note="3'-ITR" misc_feature 3143..3165 /note="TEV site" source 3166..4776 /dnas_title="GALNTL6-pGEn1-DEST" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:44629 IMAGE:5301536" /tissue_type="Brain, hypothalamus" /clone_lib="NIH_MGC_96" /lab_host="DH10B" /note="Vector: pBluescriptR" gene 3166..4776 /dnas_title="GALNTL6" /gene="GALNTL6" /gene_synonym="GALNT17" /db_xref="GeneID:442117" /db_xref="HGNC:33844" CDS 3166..4776 /dnas_title="GALNTL6" /gene="GALNTL6" /gene_synonym="GALNT17" /codon_start=1 /product="UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase-like 6" /protein_id="AAH47551.1" /db_xref="GI:71297071" /db_xref="GeneID:442117" /db_xref="HGNC:33844" /translation="MKRKQKRFLQMTLLFTVALIFLPNVGLWSLYKDKHLVKSAEPGE QQTFPLGLGDGQFYSWTDGLRRKDWHDYESIQKEAMRSRKGEHGKPYPLTEEDHDDSA YRENGFNIFVSNNIALERSLPDIRHANCKHKMYLERLPNTSIIIPFHNEGWTSLLRTI HSIINRTPGSLIAEIILVDDFSEREHLKDKLEEYMARFSKVRIVRTKKREGLIRTRLL GASMARGEVLTFLDSHCEVNVNWLPPLLNQIPLNHKTIVCPMIDVIDHNHFGYEAQAG DAMRGAFDWEMYYKRIPIPPELQRADPSDPFESPVMAGGLFAVDRKWFWELGGYDPGL EIWGGEQYEISFKVWMCGGEMFDVPCSRVGHIYRKYVPYKVPSGTSLARNLKRVAETW MDEFAEYIYQRRPEYRHLSTGDISAQKELRKQLKCKDFKWFMAAVAWDVPKYYPPVEP PPAAWGEIRNVAANLCVDSKHGATGTELRLDICVKDGSERTWSHEQLFTFGWREDIRP GEPLHTRKFCFDAISHNSPVTLYDCHGMKGNQLWGYRKDRTLFHPVSNSCMDCNHAEK KIFMARCDPLSETQQWIFEHINMTVLEKFNHHANS" misc_difference 3220..3220 /dnas_title="GALNTL6" /gene="GALNTL6" /gene_synonym="GALNT17" /note="'A' in cDNA is 'G' in the human genome; amino acid difference: 'R' in cDNA, 'G' in the human genome. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." misc_feature 3166..4776 /note="GALNTL6 coding region" misc_difference 3721..3721 /dnas_title="GALNTL6" /gene="GALNTL6" /gene_synonym="GALNT17" /note="'C' in cDNA is 'G' in the human genome; amino acid difference: 'P' in cDNA, 'A' in the human genome. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." misc_difference 4661..4661 /dnas_title="GALNTL6" /gene="GALNTL6" /gene_synonym="GALNT17" /note="'A' in cDNA is 'C' in the human genome; amino acid difference: 'H' in cDNA, 'P' in the human genome. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." vector join(4791..6193,1..3132) /source="pGEn1-DEST" /type="Destination Vector" clone 3133..4790 /source="GALNTL6-Nterm-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCGCCATGA GACTGCTGAC AGCCCTGTTC 3001 GCCTACTTCA TCGTGGCCCT GATCCTGGCC TTCAGCGTGT CCGCCAAGAG CATGCACCAC 3061 CACCATCACC ATCATCACAG CGCCTGGTCC CACCCCCAGT TCGAGAAAGA ATTATCAACA 3121 AGTTTGTACA AAAAAGCAGG CTctgaaaac ttgtactttc aaggcagaag aaaggactgg 3181 catgactatg aaagcattca gaaagaggct atgcgctcaa ggaaaggtga acatgggaaa 3241 ccttaccccc ttactgaaga ggaccatgat gactcagctt acagggaaaa tggttttaat 3301 attttcgtca gcaacaatat tgctctagag aggtctctgc cagatattcg tcatgctaac 3361 tgtaagcata agatgtatct ggaaaggctg ccaaacacca gcatcattat cccatttcat 3421 aatgaaggtt ggacttcact cctgcggacc atacacagta taattaaccg aaccccaggg 3481 agtctgatag cagaaatcat tctagtagat gacttcagtg agagagaaca cctgaaggat 3541 aaattggaag aatacatggc ccgattttcc aaagtgagga ttgttcgcac caagaaaaga 3601 gaaggactca tccggacccg tctcctgggg gcatctatgg ccagaggaga agtcctgaca 3661 ttcctggact cccactgcga ggtcaatgtg aactggctgc ccccactcct caaccaaatt 3721 ccactaaacc acaaaaccat cgtgtgtccc atgatcgatg tcattgacca caatcacttc 3781 gggtatgagg cacaagctgg ggatgccatg cgaggagcct tcgactggga aatgtactac 3841 aaaagaatcc ccatccctcc agagctccag agggcagatc ccagcgaccc ttttgagtct 3901 cctgttatgg ctggaggtct ctttgctgtg gatcggaaat ggttttggga attgggtggc 3961 tatgatccag gtttagaaat ctggggagga gaacagtatg aaatctcttt taaggtttgg 4021 atgtgtggag gagaaatgtt tgatgttcca tgttctagag ttggtcatat ctacaggaag 4081 tacgttccat acaaagttcc atctgggaca agcctggcaa gaaacctgaa gcgggtagct 4141 gagacctgga tggatgaatt tgccgagtac atttaccagc ggcggccgga gtacaggcat 4201 ctctccacgg gggacatctc tgcccagaag gagctgcgca agcagctcaa gtgcaaggac 4261 ttcaaatggt tcatggctgc tgtggcctgg gacgtgccta aatactaccc tccagtggag 4321 cccccgcctg ctgcctgggg ggagatccga aatgtggcag caaatctctg tgtggacagc 4381 aagcatggag ccaccggaac agagctgagg ctggacatct gtgtcaagga tggttctgaa 4441 agaacatggt ctcatgaaca gctttttacc tttggatgga gagaagatat tcgacccggt 4501 gagccactgc atacccggaa attctgcttt gatgcgatct cacacaacag ccccgttaca 4561 ctctatgact gtcatggcat gaaggggaac cagctctggg gataccggaa ggacagaaca 4621 ttattccatc ctgtgagcaa cagctgcatg gattgcaacc acgcagagaa gaagattttc 4681 atggccagat gtgaccctct ctctgagact cagcagtgga tttttgaaca cattaatatg 4741 actgttttag aaaaatttaa ccaccatgcc aactccTAGG ACCCAGCTTT CTTGTACAAA 4801 GTGGTTGATA GCTTGGATCC AATCAACCTC TGGATTACAA AATTTGTGAA AGATTGACTG 4861 GTATTCTTAA CTATGTTGCT CCTTTTACGC TATGTGGATA CGCTGCTTTA ATGCCTTTGT 4921 ATCATGCTAT TGCTTCCCGT ATGGCTTTCA TTTTCTCCTC CTTGTATAAA TCCTGGTTGC 4981 TGTCTCTTTA TGAGGAGTTG TGGCCCGTTG TCAGGCAACG TGGCGTGGTG TGCACTGTGT 5041 TTGCTGACGC AACCCCCACT GGTTGGGGCA TTGCCACCAC CTGTCAGCTC CTTTCCGGGA 5101 CTTTCGCTTT CCCCCTCCCT ATTGCCACGG CGGAACTCAT CGCCGCCTGC CTTGCCCGCT 5161 GCTGGACAGG GGCTCGGCTG TTGGGCACTG ACAATTCCGT GGTGTTGTCG GGGAAGCTGA 5221 CGTCCTTTCC ATGGCTGCTC GCCTGTGTTG CCACCTGGAT TCTGCGCGGG ACGTCCTTCT 5281 GCTACGTCCC TTCGGCCCTC AATCCAGCGG ACCTTCCTTC CCGCGGCCTG CTGCCGGCTC 5341 TGCGGCCTCT TCCGCGTCTT CGAGATCTGC CTCGACTGTG CCTTCTAGTT GCCAGCCATC 5401 TGTTGTTTGC CCCTCCCCCG TGCCTTCCTT GACCCTGGAA GGTGCCACTC CCACTGTCCT 5461 TTCCTAATAA AATGAGGAAA TTGCATCGCA TTGTCTGAGT AGGTGTCATT CTATTCTGGG 5521 GGGTGGGGTG GGGCAGGACA GCAAGGGGGA GGATTGGGAA GACAATAGCA GGCATGCTGG 5581 GGACTCGAGT TAAGGGCGAA TTGCCGATTA GGATCTTCCT AGAGCATGGC TACGTAGATA 5641 AGTAGCATGG CGGGTTAATC ATTAACTACA AGGAACCCCT AGTGATGGAG TTGGCCACTC 5701 CCTCTCTGCG CGCTCGCTCG CTCACTGAGG CCGGGCGACC AAAGGTCGCC CGACGCCCGG 5761 GCTTTGCCCG GGCGGCCTCA GTGAGCGAGC GAGCGCGCAG CCTTAATTAA GAGCTCATGG 5821 CGCGCCTAGG CCTTGACGGC CTTCCGCCAA TTCGCCCTAT AGTGAGTCGT ATTACGTCGC 5881 GCTCACTGGC CGTCGTTTTA CAACGTCGTG ACTGGGAAAA CCCTGGCGTT ACCCAACTTA 5941 ATCGCCTTGC AGCACATCCC CCTTTCGCCA GCTGGCGTAA TAGCGAAGAG GCCCGCACCG 6001 AAACGCCCTT CCCAACAGTT GCGCAGCCTG AATGGCGAAT GGGAGCGCCC TGTAGCGGCC 6061 ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA 6121 TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTTAACA AAATATTAAC 6181 GCTTACAATT TAG //