LOCUS ST3GAL2-pGEn1-DE 5458 bp DNA circular 22-JAN-2013 FEATURES Location/Qualifiers CDS 129..1108 /dnas_title="AmpR" /label=AmpR /vntifkey="4" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_feature 2977..3054 /note="TCM Sig Seq" misc_recomb complement(3114..3142) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_feature 3055..3078 /note="8xHis Tag" misc_feature 3085..3108 /note="StrepTag" misc_feature 4086..4627 /note="WPRE" misc_recomb 4048..4070 /label=attB2 /note="attB2 site" /note="recognized as attB2" misc_feature complement(4634..4848) /note="BGH pA" misc_feature complement(4936..5065) /note="3'-ITR" misc_feature 3143..3165 /note="TEV site" source 3166..4041 /dnas_title="ST3GAL2-pGEn1-DEST" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:46129 IMAGE:5750330" /tissue_type="Lung, Spleen, fetal, pooled" /clone_lib="NIH_MGC_122" /lab_host="DH10B" /note="Vector: pCMV-SPORT6" gene 3166..4041 /dnas_title="ST3GAL2" /gene="ST3GAL2" /gene_synonym="Gal-NAc6S" /gene_synonym="ST3GalA.2" /gene_synonym="ST3GALII" /db_xref="GeneID:6483" /db_xref="HGNC:10863" /db_xref="MIM:607188" misc_feature 3166..4041 /dnas_title="ST3GAL2" /gene="ST3GAL2" /gene_synonym="Gal-NAc6S" /gene_synonym="ST3GalA.2" /gene_synonym="ST3GALII" /note="; This cDNA sequence aligns to the human reference genome (build 36) without differences" CDS 3166..4041 /dnas_title="ST3 beta-galactoside alpha-2,3-sialyltransferase 2" /gene="ST3GAL2" /gene_synonym="Gal-NAc6S" /gene_synonym="ST3GalA.2" /gene_synonym="ST3GALII" /codon_start=1 /product="ST3 beta-galactoside alpha-2,3-sialyltransferase 2" /protein_id="AAH36777.1" /db_xref="GI:23331133" /db_xref="GeneID:6483" /db_xref="HGNC:10863" /db_xref="MIM:607188" /translation="MKCSLRVWFLSVAFLLVFIMSLLFTYSHHSMATLPYLDSGALDG THRVKLVPGYAGLQRLSKERLSGKSCACRRCMGDAGASDWFDSHFDGNISPVWTRENM DLPPDVQRWWMMLQPQFKSHNTNEVLEKLFQIVPGENPYRFRDPHQCRRCAVVGNSGN LRGSGYGQDVDGHNFIMRMNQAPTVGFEQDVGSRTTHHFMYPESAKNLPANVSFVLVP FKVLDLLWIASALSTGQIRFTYAPVKSFLRVDKEKVQIYNPAFFKYIHDRWTEHHGRY PSTGMLVLFFALHVCDEVNVYGFGADSRGNWHHYWENNRYAGEFRKTGVHDADFEAHI IDMLAKASKIEVYRGN" vector join(4056..5458,1..3132) /source="pGEn1-DEST" /type="Destination Vector" clone 3133..4055 /source="ST3GAL2-Nterm-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCGCCATGA GACTGCTGAC AGCCCTGTTC 3001 GCCTACTTCA TCGTGGCCCT GATCCTGGCC TTCAGCGTGT CCGCCAAGAG CATGCACCAC 3061 CACCATCACC ATCATCACAG CGCCTGGTCC CACCCCCAGT TCGAGAAAGA ATTATCAACA 3121 AGTTTGTACA AAAAAGCAGG CTctgaaaac ttgtactttc aaggccgcct cagcaaggag 3181 aggctctcgg gcaagagctg tgcctgtcgc cgctgcatgg gcgatgccgg tgcctccgac 3241 tggtttgaca gccactttga cggtaacatt tcccccgtct ggacccgaga gaacatggat 3301 cttccaccgg acgtccagag gtggtggatg atgctgcagc cccagttcaa gtcacacaac 3361 accaatgagg tgctggagaa gctgttccag atagtgcctg gcgagaaccc ctaccgcttc 3421 cgggaccccc accagtgccg gcgctgtgcc gtggtgggga actcgggcaa cctgcggggc 3481 tctggctatg ggcaggacgt ggacgggcac aacttcatca tgaggatgaa tcaggcgcca 3541 accgtgggct ttgagcagga tgttggcagc cgaaccaccc accatttcat gtaccctgag 3601 agtgccaaga acctgcccgc caacgtcagc ttcgtgctgg tgcccttcaa ggtcctggac 3661 cttctgtgga tcgccagcgc cttgtccacg gggcagatcc gattcaccta cgccccagtg 3721 aagtccttcc ttcgagtgga taaagaaaag gtccagatct acaacccagc cttcttcaag 3781 tatatccacg acaggtggac agagcatcac gggcggtacc cttccacggg gatgctggtg 3841 cttttctttg ccctgcatgt gtgtgatgag gtgaacgtgt acgggttcgg ggccgacagc 3901 cggggcaact ggcaccacta ctgggagaac aaccggtacg cgggcgagtt ccggaagact 3961 ggcgtgcacg acgcggactt cgaggcccac atcatcgaca tgctggccaa ggccagcaag 4021 atcgaagtct accggggcaa cTAGGACCCA GCTTTCTTGT ACAAAGTGGT TGATAGCTTG 4081 GATCCAATCA ACCTCTGGAT TACAAAATTT GTGAAAGATT GACTGGTATT CTTAACTATG 4141 TTGCTCCTTT TACGCTATGT GGATACGCTG CTTTAATGCC TTTGTATCAT GCTATTGCTT 4201 CCCGTATGGC TTTCATTTTC TCCTCCTTGT ATAAATCCTG GTTGCTGTCT CTTTATGAGG 4261 AGTTGTGGCC CGTTGTCAGG CAACGTGGCG TGGTGTGCAC TGTGTTTGCT GACGCAACCC 4321 CCACTGGTTG GGGCATTGCC ACCACCTGTC AGCTCCTTTC CGGGACTTTC GCTTTCCCCC 4381 TCCCTATTGC CACGGCGGAA CTCATCGCCG CCTGCCTTGC CCGCTGCTGG ACAGGGGCTC 4441 GGCTGTTGGG CACTGACAAT TCCGTGGTGT TGTCGGGGAA GCTGACGTCC TTTCCATGGC 4501 TGCTCGCCTG TGTTGCCACC TGGATTCTGC GCGGGACGTC CTTCTGCTAC GTCCCTTCGG 4561 CCCTCAATCC AGCGGACCTT CCTTCCCGCG GCCTGCTGCC GGCTCTGCGG CCTCTTCCGC 4621 GTCTTCGAGA TCTGCCTCGA CTGTGCCTTC TAGTTGCCAG CCATCTGTTG TTTGCCCCTC 4681 CCCCGTGCCT TCCTTGACCC TGGAAGGTGC CACTCCCACT GTCCTTTCCT AATAAAATGA 4741 GGAAATTGCA TCGCATTGTC TGAGTAGGTG TCATTCTATT CTGGGGGGTG GGGTGGGGCA 4801 GGACAGCAAG GGGGAGGATT GGGAAGACAA TAGCAGGCAT GCTGGGGACT CGAGTTAAGG 4861 GCGAATTGCC GATTAGGATC TTCCTAGAGC ATGGCTACGT AGATAAGTAG CATGGCGGGT 4921 TAATCATTAA CTACAAGGAA CCCCTAGTGA TGGAGTTGGC CACTCCCTCT CTGCGCGCTC 4981 GCTCGCTCAC TGAGGCCGGG CGACCAAAGG TCGCCCGACG CCCGGGCTTT GCCCGGGCGG 5041 CCTCAGTGAG CGAGCGAGCG CGCAGCCTTA ATTAAGAGCT CATGGCGCGC CTAGGCCTTG 5101 ACGGCCTTCC GCCAATTCGC CCTATAGTGA GTCGTATTAC GTCGCGCTCA CTGGCCGTCG 5161 TTTTACAACG TCGTGACTGG GAAAACCCTG GCGTTACCCA ACTTAATCGC CTTGCAGCAC 5221 ATCCCCCTTT CGCCAGCTGG CGTAATAGCG AAGAGGCCCG CACCGAAACG CCCTTCCCAA 5281 CAGTTGCGCA GCCTGAATGG CGAATGGGAG CGCCCTGTAG CGGCCACTCA ACCCTATCTC 5341 GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA 5401 GCTGATTTAA CAAAAATTTA ACGCGAATTT TAACAAAATA TTAACGCTTA CAATTTAG //