LOCUS       pDONR™/Zeo
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FEATURES             Location/Qualifiers
     misc_feature    complement(268..295)
                     /note="rrnB T2 transcription termination sequence (c)"
     misc_feature    complement(427..470)
                     /note="rrnB T1 transcription termination sequence (c)"
     primer_bind     537..552
                     /note="M13 Forward (-20) priming site"
     misc_recomb     570..668
                     /label=attL1
                     /note="attL1"
     misc_recomb     complement(1583..1679)
                     /label=attL2
                     /note="attL2 (c)"
     misc_signal     complement(1694..1713)
                     /note="T7 Promoter/priming site (c)"
     primer_bind     1721..1737
                     /note="M13 Reverse priming site"
     gene            1850..2659
                     /note="Kanamycin resistance gene"
     rep_origin      2780..3453
                     /note="pUC origin"
     vector          join(1591..3456,1..651)
                     /source="pDONR%99221"
                     /type="Donor Vector"
     misc_feature    669..691
                     /note="TEV site"
     source          692..1576
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /clone="MGC:29856 IMAGE:4996181"
                     /tissue_type="Placenta, choriocarcinoma"
                     /clone_lib="NIH_MGC_10"
                     /lab_host="DH10B"
                     /note="Vector: pCMV-SPORT6"
     gene            692..1576
                     /gene="ST3GAL6"
                     /gene_synonym=ST3GALVI
                     /db_xref="GeneID:10402"
                     /db_xref="HGNC:18080"
                     /db_xref="MIM:607156"
     CDS             692..1576
                     /dnas_title="ST3 beta-galactoside
                     alpha-2,3-sialyltransferase 6"
                     /gene="ST3GAL6"
                     /gene_synonym=ST3GALVI
                     /codon_start=1
                     /product="ST3 beta-galactoside
                     alpha-2,3-sialyltransferase 6"
                     /protein_id="AAH23312.1"
                     /db_xref="GI:23271058"
                     /db_xref="GeneID:10402"
                     /db_xref="HGNC:18080"
                     /db_xref="MIM:607156"
                     /translation="MRGYLVAIFLSAVFLYYVLHCILWGTNVYWVAPVEMKRRNKIQP
                     CLSKPAFASLLRFHQFHPFLCAADFRKIASLYGSDKFDLPYGMRTSAEYFRLALSKLQ
                     SCDLFDEFDNIPCKKCVVVGNGGVLKNKTLGEKIDSYDVIIRMNNGPVLGHEEEVGRR
                     TTFRLFYPESVFSDPIHNDPNTTVILTAFKPHDLRWLLELLMGDKINTNGFWKKPALN
                     LIYKPYQIRILDPFIIRTAAYELLHFPKVFPKNQKPKHPTTGIIAITLAFYICHEVHL
                     AGFKYNFSDLKSPLHYYGNATMSLMNKNAYHNVTAEQLFLKDIIEKNLVINLTQD"
ORIGIN      
          1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
         61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
        121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
        181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC
        241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA
        301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC
        361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA
        421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG
        481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA
        541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC
        601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA
        661 AGCAGGCTct gaaaacttgt actttcaagg caaacggaga aataagatcc agccttgttt
        721 atcaaagcca gcttttgcct ctctgctgag gtttcatcag tttcaccctt ttctgtgtgc
        781 ggctgatttt agaaagattg cttccttgta tggtagcgat aagtttgatt tgccctatgg
        841 gatgagaaca tcagcggaat attttcgact tgctctttca aaactgcaga gttgtgatct
        901 ctttgatgag tttgacaaca taccctgtaa aaagtgtgtg gtggttggta atggaggagt
        961 tttgaagaat aagacattag gagaaaaaat cgactcctat gatgtaataa taagaatgaa
       1021 taatggtcct gttttaggac atgaagaaga agttgggaga aggacaacct tccgactttt
       1081 ttatccagaa tctgtttttt cagatcctat tcacaatgac cctaatacga cagtgattct
       1141 cactgctttt aagccacatg atttaaggtg gctgttggaa ttgttgatgg gtgacaaaat
       1201 aaacactaat ggtttttgga agaaaccagc cttaaacctg atttataaac cttatcaaat
       1261 ccgaatatta gatcctttca ttatcagaac agcagcttat gaactgcttc attttccaaa
       1321 agtgtttccc aaaaatcaga aacctaaaca cccaacaaca ggaattattg ccatcacatt
       1381 ggcgttttac atatgtcacg aagttcacct agctggtttt aaatacaact tttctgacct
       1441 caagagtcct ttgcactact atgggaatgc caccatgtct ttgatgaata agaacgcgta
       1501 tcacaatgtg actgcagagc agctcttttt gaaggacatt atagaaaaaa acctcgtaat
       1561 caacttgact caagatTAGG ACCCAGCTTT CTTGTACAAA GTTGGCATTA TAAGAAAGCA
       1621 TTGCTTATCA ATTTGTTGCA ACGAACAGGT CACTATCAGT CAAAATAAAA TCATTATTTG
       1681 CCATCCAGCT GATATCCCCT ATAGTGAGTC GTATTACATG GTCATAGCTG TTTCCTGGCA
       1741 GCTCTGGCCC GTGTCTCAAA ATCTCTGATG TTACATTGCA CAAGATAAAA TAATATCATC
       1801 ATGAACAATA AAACTGTCTG CTTACATAAA CAGTAATACA AGGGGTGTTA TGAGCCATAT
       1861 TCAACGGGAA ACGTCGAGGC CGCGATTAAA TTCCAACATG GATGCTGATT TATATGGGTA
       1921 TAAATGGGCT CGCGATAATG TCGGGCAATC AGGTGCGACA ATCTATCGCT TGTATGGGAA
       1981 GCCCGATGCG CCAGAGTTGT TTCTGAAACA TGGCAAAGGT AGCGTTGCCA ATGATGTTAC
       2041 AGATGAGATG GTCAGACTAA ACTGGCTGAC GGAATTTATG CCTCTTCCGA CCATCAAGCA
       2101 TTTTATCCGT ACTCCTGATG ATGCATGGTT ACTCACCACT GCGATCCCCG GAAAAACAGC
       2161 ATTCCAGGTA TTAGAAGAAT ATCCTGATTC AGGTGAAAAT ATTGTTGATG CGCTGGCAGT
       2221 GTTCCTGCGC CGGTTGCATT CGATTCCTGT TTGTAATTGT CCTTTTAACA GCGATCGCGT
       2281 ATTTCGTCTC GCTCAGGCGC AATCACGAAT GAATAACGGT TTGGTTGATG CGAGTGATTT
       2341 TGATGACGAG CGTAATGGCT GGCCTGTTGA ACAAGTCTGG AAAGAAATGC ATAAACTTTT
       2401 GCCATTCTCA CCGGATTCAG TCGTCACTCA TGGTGATTTC TCACTTGATA ACCTTATTTT
       2461 TGACGAGGGG AAATTAATAG GTTGTATTGA TGTTGGACGA GTCGGAATCG CAGACCGATA
       2521 CCAGGATCTT GCCATCCTAT GGAACTGCCT CGGTGAGTTT TCTCCTTCAT TACAGAAACG
       2581 GCTTTTTCAA AAATATGGTA TTGATAATCC TGATATGAAT AAATTGCAGT TTCATTTGAT
       2641 GCTCGATGAG TTTTTCTAAT CAGAATTGGT TAATTGGTTG TAACACTGGC AGAGCATTAC
       2701 GCTGACTTGA CGGGACGGCG CAAGCTCATG ACCAAAATCC CTTAACGTGA GTTACGCGTC
       2761 GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT
       2821 TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT
       2881 GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT
       2941 ACCAAATACT GTTCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC
       3001 ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA
       3061 GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG
       3121 CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG
       3181 ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG
       3241 GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA
       3301 CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT
       3361 GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG
       3421 GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTT
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