LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(1646..1742) /label=attL2 /note="attL2 (c)" misc_signal complement(1757..1776) /note="T7 Promoter/priming site (c)" primer_bind 1784..1800 /note="M13 Reverse priming site" gene 1913..2722 /note="Kanamycin resistance gene" rep_origin 2843..3516 /note="pUC origin" vector join(1654..3519,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 669..691 /note="TEV site" source 692..1639 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:116854 IMAGE:40004644" /tissue_type="PCR rescued clones" /clone_lib="NIH_MGC_282" /note="Vector: pCR-Blunt II-TOPO; Clone identification sequence tag: GAATATCA sequenced from the reverse primer" gene 692..1639 /gene="ST8SIA2" /gene_synonym=HsT19690 /gene_synonym=ST8SIA-II /gene_synonym=STX /db_xref="GeneID:8128" /db_xref="HGNC:10870" /db_xref="MIM:602546" CDS 692..1639 /dnas_title="ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 2" /gene="ST8SIA2" /gene_synonym=HsT19690 /gene_synonym=ST8SIA-II /gene_synonym=STX /codon_start=1 /product="ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 2" /protein_id="AAH96202.1" /db_xref="GI:64653156" /db_xref="GeneID:8128" /db_xref="HGNC:10870" /db_xref="MIM:602546" /translation="MQLQFRSWMLAALTLLVVFLIFADISEIEEEIGNSGGRGTIRSA VNSLHSKSNRAEVVINGSSSPAVVDRSNESIKHNIQPASSKWRHNQTLSLRIRKQILK FLDAEKDISVLKGTLKPGDIIHYIFDRDSTMNVSQNLYELLPRTSPLKNKHFGTCAIV GNSGVLLNSGCGQEIDAHSFVIRCNLAPVQEYARDVGLKTDLVTMNPSVIQRAFEDLV NATWREKLLQRLHSLNGSILWIPAFMARGGKERVEWVNELILKHHVNVRTAYPSLRLL HAVRGYWLTNKVHIKRPTTGLLMYTLATRFCKQIYLYGFWPFPLDQNQNPVKYHYYDS LKYGYTSQASAHTMPLEFKALKSLHEQGALKLTVGQCDGAT" misc_difference 1135..1135 /gene="ST8SIA2" /gene_synonym=HsT19690 /gene_synonym=ST8SIA-II /gene_synonym=STX /note="'G' in cDNA is 'C' in the human genome; no amino acid change. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." misc_difference 1547..1547 /gene="ST8SIA2" /gene_synonym=HsT19690 /gene_synonym=ST8SIA-II /gene_synonym=STX /note="'G' in cDNA is 'C' in the human genome; amino acid difference: 'A' in cDNA, 'P' in the human genome. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg caacggctcc tcatcaccag ctgttgttga 721 cagaagtaat gaaagcatca agcacaacat ccagccagcc tcgtccaaat ggagacataa 781 ccagacgctc tctctgagga tcaggaagca gattttaaag ttcttggatg ctgaaaagga 841 catttctgtc ctaaagggaa ccctgaagcc tggagatatt attcattaca tcttcgatcg 901 agacagcacc atgaatgtgt cccagaacct ctacgagctc ctccccagga cttcgccact 961 gaagaataag cactttggga cttgtgccat cgtgggcaac tcgggggtct tgctgaacag 1021 cggctgtggg caggagattg acgcccacag cttcgtcatc aggtgcaacc tggccccagt 1081 acaggagtat gcccgggatg tggggctcaa gacagacctg gtaaccatga acccgtcggt 1141 catccagcgg gcctttgagg acttggtcaa tgccacgtgg cgggagaagc tgctgcaacg 1201 gctgcacagc ctcaatggca gcatcctgtg gatccctgcc ttcatggccc ggggcggcaa 1261 ggagcgtgtt gagtgggtca acgagcttat cctgaagcac cacgtcaacg tgcgcactgc 1321 atacccctcg ctgcgcctgc tgcacgccgt tcgcggatac tggctgacca acaaagtcca 1381 catcaaaaga cccaccaccg gcctcttgat gtataccctg gccacacgtt tctgcaaaca 1441 aatctacctc tacggcttct ggccctttcc gctggatcag aaccagaacc cagtcaagta 1501 ccactattat gacagcctca agtatggcta cacctcccag gccagcgcgc ataccatgcc 1561 cttggagttt aaggccctca agagcctaca tgagcagggg gctttgaaac tgactgtcgg 1621 ccagtgcgat ggggccacgT AGGACCCAGC TTTCTTGTAC AAAGTTGGCA TTATAAGAAA 1681 GCATTGCTTA TCAATTTGTT GCAACGAACA GGTCACTATC AGTCAAAATA AAATCATTAT 1741 TTGCCATCCA GCTGATATCC CCTATAGTGA GTCGTATTAC ATGGTCATAG CTGTTTCCTG 1801 GCAGCTCTGG CCCGTGTCTC AAAATCTCTG ATGTTACATT GCACAAGATA AAATAATATC 1861 ATCATGAACA ATAAAACTGT CTGCTTACAT AAACAGTAAT ACAAGGGGTG TTATGAGCCA 1921 TATTCAACGG GAAACGTCGA GGCCGCGATT AAATTCCAAC ATGGATGCTG ATTTATATGG 1981 GTATAAATGG GCTCGCGATA ATGTCGGGCA ATCAGGTGCG ACAATCTATC GCTTGTATGG 2041 GAAGCCCGAT GCGCCAGAGT TGTTTCTGAA ACATGGCAAA GGTAGCGTTG CCAATGATGT 2101 TACAGATGAG ATGGTCAGAC TAAACTGGCT GACGGAATTT ATGCCTCTTC CGACCATCAA 2161 GCATTTTATC CGTACTCCTG ATGATGCATG GTTACTCACC ACTGCGATCC CCGGAAAAAC 2221 AGCATTCCAG GTATTAGAAG AATATCCTGA TTCAGGTGAA AATATTGTTG ATGCGCTGGC 2281 AGTGTTCCTG CGCCGGTTGC ATTCGATTCC TGTTTGTAAT TGTCCTTTTA ACAGCGATCG 2341 CGTATTTCGT CTCGCTCAGG CGCAATCACG AATGAATAAC GGTTTGGTTG ATGCGAGTGA 2401 TTTTGATGAC GAGCGTAATG GCTGGCCTGT TGAACAAGTC TGGAAAGAAA TGCATAAACT 2461 TTTGCCATTC TCACCGGATT CAGTCGTCAC TCATGGTGAT TTCTCACTTG ATAACCTTAT 2521 TTTTGACGAG GGGAAATTAA TAGGTTGTAT TGATGTTGGA CGAGTCGGAA TCGCAGACCG 2581 ATACCAGGAT CTTGCCATCC TATGGAACTG CCTCGGTGAG TTTTCTCCTT CATTACAGAA 2641 ACGGCTTTTT CAAAAATATG GTATTGATAA TCCTGATATG AATAAATTGC AGTTTCATTT 2701 GATGCTCGAT GAGTTTTTCT AATCAGAATT GGTTAATTGG TTGTAACACT GGCAGAGCAT 2761 TACGCTGACT TGACGGGACG GCGCAAGCTC ATGACCAAAA TCCCTTAACG TGAGTTACGC 2821 GTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT 2881 TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG 2941 TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA 3001 GATACCAAAT ACTGTTCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT 3061 AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA 3121 TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC 3181 GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT 3241 GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA 3301 CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG 3361 AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT 3421 TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT 3481 ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTT //