LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(1655..1751) /label=attL2 /note="attL2 (c)" misc_signal complement(1766..1785) /note="T7 Promoter/priming site (c)" primer_bind 1793..1809 /note="M13 Reverse priming site" gene 1922..2731 /note="Kanamycin resistance gene" rep_origin 2852..3525 /note="pUC origin" vector join(1663..3528,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 669..691 /note="TEV site" source 692..1648 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:103874 IMAGE:30915256" /tissue_type="Pooled tissue, PCR rescued clones" /clone_lib="NIH_MGC_271" /note="Vector: pCR4 Topo TA with reversed insert" gene 692..1648 /gene="ST8SIA3" /gene_synonym=ST8Sia III /db_xref="GeneID:51046" /db_xref="HGNC:14269" /db_xref="MIM:609478" misc_feature 692..1648 /gene="ST8SIA3" /gene_synonym=ST8Sia III /note="; This cDNA sequence aligns to the human reference genome (build 36) without differences" CDS 692..1648 /dnas_title="ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 3" /gene="ST8SIA3" /gene_synonym=ST8Sia III /codon_start=1 /product="ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 3" /protein_id="AAH74909.1" /db_xref="GI:50960135" /db_xref="GeneID:51046" /db_xref="HGNC:14269" /db_xref="MIM:609478" /translation="MRNCKMARVASVLGLVMLSVALLILSLISYVSLKKENIFTTPKY ASPGAPRMYMFHAGFRSQFALKFLDPSFVPITNSLTQELQEKPSKWKFNRTAFLHQRQ EILQHVDVIKNFSLTKNSVRIGQLMHYDYSSHKYVFSISNNFRSLLPDVSPIMNKHYN ICAVVGNSGILTGSQCGQEIDKSDFVFRCNFAPTEAFQRDVGRKTNLTTFNPSILEKY YNNLLTIQDRNNFFLSLKKLDGAILWIPAFFFHTSATVTRTLVDFFVEHRGQLKVQLA WPGNIMQHVNRYWKNKHLSPKRLSTGILMYTLASAICEEIHLYGFWPFGFDPNTREDL PYHYYDKKGTKFTTKWQESHQLPAEFQLLYRMHGEGLTKLTLSHCA" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg ccaatttgcg ctgaagtttc tagacccgtc 721 attcgtgccc attacgaatt ctctcaccca ggaactccaa gagaaacctt ctaagtggaa 781 atttaatcgg acagcgtttt tacatcaaag gcaagaaatt cttcagcatg tcgatgtaat 841 aaaaaatttt tctttgacca agaatagtgt tcggattgga caactgatgc actatgatta 901 ttccagccat aaatatgttt tctctattag caataacttc cggtcacttc ttccagatgt 961 gtcacccatt atgaacaagc attataatat ttgtgctgtg gttggaaata gtgggatcct 1021 gacagggagc cagtgtggac aagaaataga taaatcagat tttgttttcc gttgcaattt 1081 cgcccctacg gaggctttcc aaagagatgt tggaagaaaa accaatctta ccaccttcaa 1141 ccccagcatc ctggaaaaat attacaacaa tctcttgact attcaggacc gtaacaactt 1201 tttcctcagt ttaaaaaagc ttgacggggc cattctttgg atccctgcat ttttcttcca 1261 cacttcagca actgtgacca ggacattagt tgactttttt gttgaacaca gaggtcagtt 1321 aaaagtccaa ctggcttggc cgggaaatat aatgcaacat gtcaacaggt actggaaaaa 1381 caaacatttg tcacctaaac ggctgagcac aggtattctt atgtacaccc ttgcatcagc 1441 aatatgtgaa gagatccact tgtatggatt ttggccgttt ggatttgacc ccaacacaag 1501 ggaagatctt ccataccatt actatgacaa aaaaggaacc aaatttacca ccaagtggca 1561 ggagtcccac cagctgcctg ctgagtttca gctgctgtac cgaatgcatg gggaagggct 1621 caccaagctg actctgtcac actgtgccTA GGACCCAGCT TTCTTGTACA AAGTTGGCAT 1681 TATAAGAAAG CATTGCTTAT CAATTTGTTG CAACGAACAG GTCACTATCA GTCAAAATAA 1741 AATCATTATT TGCCATCCAG CTGATATCCC CTATAGTGAG TCGTATTACA TGGTCATAGC 1801 TGTTTCCTGG CAGCTCTGGC CCGTGTCTCA AAATCTCTGA TGTTACATTG CACAAGATAA 1861 AATAATATCA TCATGAACAA TAAAACTGTC TGCTTACATA AACAGTAATA CAAGGGGTGT 1921 TATGAGCCAT ATTCAACGGG AAACGTCGAG GCCGCGATTA AATTCCAACA TGGATGCTGA 1981 TTTATATGGG TATAAATGGG CTCGCGATAA TGTCGGGCAA TCAGGTGCGA CAATCTATCG 2041 CTTGTATGGG AAGCCCGATG CGCCAGAGTT GTTTCTGAAA CATGGCAAAG GTAGCGTTGC 2101 CAATGATGTT ACAGATGAGA TGGTCAGACT AAACTGGCTG ACGGAATTTA TGCCTCTTCC 2161 GACCATCAAG CATTTTATCC GTACTCCTGA TGATGCATGG TTACTCACCA CTGCGATCCC 2221 CGGAAAAACA GCATTCCAGG TATTAGAAGA ATATCCTGAT TCAGGTGAAA ATATTGTTGA 2281 TGCGCTGGCA GTGTTCCTGC GCCGGTTGCA TTCGATTCCT GTTTGTAATT GTCCTTTTAA 2341 CAGCGATCGC GTATTTCGTC TCGCTCAGGC GCAATCACGA ATGAATAACG GTTTGGTTGA 2401 TGCGAGTGAT TTTGATGACG AGCGTAATGG CTGGCCTGTT GAACAAGTCT GGAAAGAAAT 2461 GCATAAACTT TTGCCATTCT CACCGGATTC AGTCGTCACT CATGGTGATT TCTCACTTGA 2521 TAACCTTATT TTTGACGAGG GGAAATTAAT AGGTTGTATT GATGTTGGAC GAGTCGGAAT 2581 CGCAGACCGA TACCAGGATC TTGCCATCCT ATGGAACTGC CTCGGTGAGT TTTCTCCTTC 2641 ATTACAGAAA CGGCTTTTTC AAAAATATGG TATTGATAAT CCTGATATGA ATAAATTGCA 2701 GTTTCATTTG ATGCTCGATG AGTTTTTCTA ATCAGAATTG GTTAATTGGT TGTAACACTG 2761 GCAGAGCATT ACGCTGACTT GACGGGACGG CGCAAGCTCA TGACCAAAAT CCCTTAACGT 2821 GAGTTACGCG TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG 2881 AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC 2941 GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG 3001 CAGAGCGCAG ATACCAAATA CTGTTCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA 3061 GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC 3121 CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC 3181 GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA 3241 CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG 3301 AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT 3361 TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA 3421 GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC 3481 GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTT //