LOCUS       pDONR™/Zeo
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FEATURES             Location/Qualifiers
     misc_feature    complement(268..295)
                     /note="rrnB T2 transcription termination sequence (c)"
     misc_feature    complement(427..470)
                     /note="rrnB T1 transcription termination sequence (c)"
     primer_bind     537..552
                     /note="M13 Forward (-20) priming site"
     misc_recomb     570..668
                     /label=attL1
                     /note="attL1"
     misc_recomb     complement(1772..1868)
                     /label=attL2
                     /note="attL2 (c)"
     misc_signal     complement(1883..1902)
                     /note="T7 Promoter/priming site (c)"
     primer_bind     1910..1926
                     /note="M13 Reverse priming site"
     gene            2039..2848
                     /note="Kanamycin resistance gene"
     rep_origin      2969..3642
                     /note="pUC origin"
     vector          join(1780..3645,1..651)
                     /source="pDONR%99221"
                     /type="Donor Vector"
     misc_feature    669..691
                     /note="TEV site"
     source          692..1765
                     /organism="synthetic construct"
                     /mol_type="other DNA"
                     /db_xref="taxon:32630"
                     /clone="MGC:183157 IMAGE:100015708"
                     /clone_lib="NIH_MGC_428"
                     /lab_host="DH10B"
                     /focus
                     /note="Vector: pENTR223.1 with stop codon"
     source          692..1765
                     /organism="Homo sapiens"
                     /mol_type="other DNA"
                     /db_xref="taxon:9606"
     gene            692..1765
                     /gene="B3GNT7"
                     /gene_synonym=beta3GnT7
                     /db_xref="GeneID:93010"
                     /db_xref="HGNC:18811"
     CDS             692..1765
                     /dnas_title="UDP-GlcNAc:betaGal
                     beta-1,3-N-acetylglucosaminyltransferase 7"
                     /gene="B3GNT7"
                     /gene_synonym=beta3GnT7
                     /codon_start=1
                     /product="UDP-GlcNAc:betaGal
                     beta-1,3-N-acetylglucosaminyltransferase 7"
                     /protein_id="AAI48681.1"
                     /db_xref="GI:151555517"
                     /db_xref="GeneID:93010"
                     /db_xref="HGNC:18811"
                     /translation="MSLWKKTVYRSLCLALALLVAVTVFQRSLTPGQFLQEPPPPTLE
                     PQKAQKPNGQLVNPNNFWKNPKDVAAPTPMASQGPQAWDVTTTNCSANINLTHQPWFQ
                     VLEPQFRQFLFYRHCRYFPMLLNHPEKCRGDVYLLVVVKSVITQHDRREAIRQTWGRE
                     RQSAGGGRGAVRTLFLLGTASKQEERTHYQQLLAYEDRLYGDILQWGFLDTFFNLTLK
                     EIHFLKWLDIYCPHVPFIFKGDDDVFVNPTNLLEFLADRQPQENLFVGDVLQHARPIR
                     RKDNKYYIPGALYGKASYPPYAGGGGFLMAGSLARRLHHACDTLELYPIDDVFLGMCL
                     EVLGVQPTAHEGFKTFGISRNRNSRMNKEPCFFRAMLVVHKLLPPELLAMWGLVHSNL
                     TCSRKLQVL"
     misc_feature    692..1765
                     /note="B3GNT7 coding region"
ORIGIN      
          1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
         61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
        121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
        181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC
        241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA
        301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC
        361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA
        421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG
        481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA
        541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC
        601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA
        661 AGCAGGCTct gaaaacttgt actttcaagg cgagccacag aaggcccaga agccaaatgg
        721 acagctggtg aaccccaaca acttctggaa gaacccgaaa gatgtggctg cgcccacgcc
        781 catggcctct caggggcccc aggcctggga cgtgaccacc actaactgct cagccaatat
        841 caacttgacc caccagccct ggttccaggt cctggagccg cagttccggc agtttctctt
        901 ctaccgccac tgccgctact tccccatgct gctgaaccac ccggagaagt gcaggggcga
        961 tgtctacctg ctggtggttg tcaagtcggt catcacgcag cacgaccgcc gcgaggccat
       1021 ccgccagacc tggggccgcg agcggcagtc cgcgggtggg ggccgaggcg ccgtgcgcac
       1081 cctcttcctg ctgggcacgg cctccaagca ggaggagcgc acgcactacc agcagctgct
       1141 ggcctacgaa gaccgcctct acggcgacat cctgcagtgg ggctttctcg acaccttctt
       1201 caacctgacc ctcaaggaga tccacttcct caagtggctg gacatctact gcccccacgt
       1261 ccccttcatt ttcaaaggcg acgatgacgt cttcgtcaac cccaccaacc tgctagaatt
       1321 tctggctgac cggcagccac aggaaaacct gttcgtgggc gatgtcctgc agcacgctcg
       1381 gcccattcgc aggaaagaca acaaatacta catcccgggg gccctgtacg gcaaggccag
       1441 ctatccgccg tatgcaggcg gcggtggctt cctcatggcc ggcagcctgg cccggcgcct
       1501 gcaccatgcc tgcgacaccc tggagctcta cccgatcgac gacgtctttc tgggcatgtg
       1561 cctggaggtg ctgggcgtgc agcccacggc ccacgagggc ttcaagactt tcggcatctc
       1621 ccggaaccgc aacagccgca tgaacaagga gccgtgcttt ttccgcgcca tgctcgtggt
       1681 gcacaagctg ctgccccctg agctgctcgc catgtggggg ctggtgcaca gcaatctcac
       1741 ctgctcccgc aagctccagg tgctcTAGGA CCCAGCTTTC TTGTACAAAG TTGGCATTAT
       1801 AAGAAAGCAT TGCTTATCAA TTTGTTGCAA CGAACAGGTC ACTATCAGTC AAAATAAAAT
       1861 CATTATTTGC CATCCAGCTG ATATCCCCTA TAGTGAGTCG TATTACATGG TCATAGCTGT
       1921 TTCCTGGCAG CTCTGGCCCG TGTCTCAAAA TCTCTGATGT TACATTGCAC AAGATAAAAT
       1981 AATATCATCA TGAACAATAA AACTGTCTGC TTACATAAAC AGTAATACAA GGGGTGTTAT
       2041 GAGCCATATT CAACGGGAAA CGTCGAGGCC GCGATTAAAT TCCAACATGG ATGCTGATTT
       2101 ATATGGGTAT AAATGGGCTC GCGATAATGT CGGGCAATCA GGTGCGACAA TCTATCGCTT
       2161 GTATGGGAAG CCCGATGCGC CAGAGTTGTT TCTGAAACAT GGCAAAGGTA GCGTTGCCAA
       2221 TGATGTTACA GATGAGATGG TCAGACTAAA CTGGCTGACG GAATTTATGC CTCTTCCGAC
       2281 CATCAAGCAT TTTATCCGTA CTCCTGATGA TGCATGGTTA CTCACCACTG CGATCCCCGG
       2341 AAAAACAGCA TTCCAGGTAT TAGAAGAATA TCCTGATTCA GGTGAAAATA TTGTTGATGC
       2401 GCTGGCAGTG TTCCTGCGCC GGTTGCATTC GATTCCTGTT TGTAATTGTC CTTTTAACAG
       2461 CGATCGCGTA TTTCGTCTCG CTCAGGCGCA ATCACGAATG AATAACGGTT TGGTTGATGC
       2521 GAGTGATTTT GATGACGAGC GTAATGGCTG GCCTGTTGAA CAAGTCTGGA AAGAAATGCA
       2581 TAAACTTTTG CCATTCTCAC CGGATTCAGT CGTCACTCAT GGTGATTTCT CACTTGATAA
       2641 CCTTATTTTT GACGAGGGGA AATTAATAGG TTGTATTGAT GTTGGACGAG TCGGAATCGC
       2701 AGACCGATAC CAGGATCTTG CCATCCTATG GAACTGCCTC GGTGAGTTTT CTCCTTCATT
       2761 ACAGAAACGG CTTTTTCAAA AATATGGTAT TGATAATCCT GATATGAATA AATTGCAGTT
       2821 TCATTTGATG CTCGATGAGT TTTTCTAATC AGAATTGGTT AATTGGTTGT AACACTGGCA
       2881 GAGCATTACG CTGACTTGAC GGGACGGCGC AAGCTCATGA CCAAAATCCC TTAACGTGAG
       2941 TTACGCGTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA
       3001 TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT
       3061 GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG
       3121 AGCGCAGATA CCAAATACTG TTCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA
       3181 CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG
       3241 TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA
       3301 GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC
       3361 CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA
       3421 GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC
       3481 AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG
       3541 TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC
       3601 CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTT
//