LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2087..2183) /label=attL2 /note="attL2 (c)" misc_signal complement(2198..2217) /note="T7 Promoter/priming site (c)" primer_bind 2225..2241 /note="M13 Reverse priming site" gene 2354..3163 /note="Kanamycin resistance gene" rep_origin 3284..3957 /note="pUC origin" PCR_frame 669..2087 /note="Reading Frame" /set="Set 1" PCR_primer 652..668 /primer="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original=ACAAGTTTGTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC PCR_primer complement(669..2107) /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original=ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG PCR_product complement(652..2104) /pair="Pair 1" /set="Set 1" /current=1 /score=1.000 vector join(2095..3960,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 2065..2085 /note="TEV site" source 673..2064 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:1099 IMAGE:3510605" /tissue_type="Brain, neuroblastoma" /clone_lib="NIH_MGC_19" /lab_host="DH10B-R" /note="Vector: pOTB7" gene 673..2064 /gene="ALG1" /gene_synonym=HMAT1 /gene_synonym=HMT-1 /gene_synonym=HMT1 /db_xref="GeneID:56052" /db_xref="HGNC:18294" /db_xref="MIM:605907" CDS 673..2064 /dnas_title="asparagine-linked glycosylation 1, beta-1,4-mannosyltransferase homolog (S. cerevisiae)" /gene="ALG1" /gene_synonym=HMAT1 /gene_synonym=HMT-1 /gene_synonym=HMT1 /codon_start=1 /product="asparagine-linked glycosylation 1, beta-1,4-mannosyltransferase homolog (S. cerevisiae)" /protein_id="AAH04402.1" /db_xref="GI:13325168" /db_xref="GeneID:56052" /db_xref="HGNC:18294" /db_xref="MIM:605907" /translation="MAASCLVLLALCLLLPLLLLGGWKRWRRGRAARHVVAVVLGDVG RSPRMQYHALSLAMHGFSVTLLGFCNSKPHDELLQNNRIQIVGLTELQSLAVGPRVFQ YGVKVVLQAMYLLWKLMWREPGAYIFLQNPPGLPSIAVCWFVGCLCGSKLVIDWHNYG YSIMGLVHGPNHPLVLLAKWYEKFFGRLSHLNLCVTNAMREDLADNWHIRAVTVYDKP ASFFKETPLDLQHRLFMKLGSMHSPFRARSEPEDPVTERSAFTERDAGNGLVTRLRER PALLVSSTSWTEDEDFSILLAALEKFEQLTLDGHNLPSLVCVITGKGPLREYYSRLIH QKHFQHIQVCTPWLEAEDYPLLLGSADLGVCLHTSSSGLDLPMKVVDMFGCCLPVCAV NFKCLHELVKHEENGLVFEDSEELAAQLQMLFSNFPDPAGKLNQFRKNLRESQQLRWD ESWVQTVLPLVMDT" misc_feature 673..2064 /note="ALG1 coding region" misc_difference 1472..1472 /gene="ALG1" /gene_synonym=HMAT1 /gene_synonym=HMT-1 /gene_synonym=HMT1 /note="'A' in cDNA is 'G' in the human genome; amino acid difference: 'N' in cDNA, 'S' in the human genome. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTCG CCatggcggc ctcatgcttg gtcctgctgg cgctgtgtct gctgctgccg 721 ctgctgctgc tgggaggatg gaagcgctgg cgccgggggc gggcggcccg gcatgtagta 781 gcggtggtgc tgggcgacgt gggccgcagc ccccgtatgc agtaccacgc gctgtcgttg 841 gccatgcacg gcttctcggt gaccctcctg gggttctgca actccaaacc ccatgatgag 901 ctcttgcaga acaacagaat tcagattgtg gggttgacag aacttcagag tcttgcagtt 961 gggccccgag ttttccagta cggagtcaaa gttgtacttc aggctatgta cttgctgtgg 1021 aagttgatgt ggagggagcc aggtgcctat atctttctcc agaacccccc aggtctgcct 1081 agcattgctg tctgctggtt cgtgggctgc ctttgtggaa gcaagctcgt cattgactgg 1141 cacaactatg gctactccat catgggtctg gtgcatggcc ccaaccatcc cctcgttctg 1201 ctggccaagt ggtacgagaa gttctttggg cgcctgtccc acctgaacct gtgtgttacc 1261 aatgctatgc gagaagacct ggcggataac tggcacatca gggctgtgac cgtctacgac 1321 aagcccgcat ctttctttaa agagacacct ctggacctgc agcaccggct cttcatgaag 1381 ctgggcagca tgcactctcc gttcagggcc cgctcagaac ctgaggaccc agtcacggag 1441 cggtcggcct tcacggagcg ggatgctggg aacgggctgg tgacgcgtct ccgtgagcgg 1501 ccagccctgc tggtcagcag cacgagctgg acagaggacg aagacttctc catcctgctg 1561 gcagctttag aaaagtttga acaactgact cttgatggac acaaccttcc ttctctcgtc 1621 tgtgtgataa caggcaaagg gcctctgagg gagtattata gccgcctcat ccaccagaag 1681 cacttccagc acatccaggt ctgcaccccc tggctggagg ccgaggacta ccccctgctt 1741 ctagggtcgg cggacctggg tgtctgtctg cacacgtcct ccagtggcct ggacctgccc 1801 atgaaggtgg tggacatgtt cgggtgctgt ttgcctgtgt gtgctgtgaa cttcaagtgt 1861 ttacatgagc tggtgaaaca tgaagaaaat ggcctggtct ttgaggactc agaggaactg 1921 gcagctcagc tgcagatgct tttctcaaac tttcctgatc ctgcgggcaa gctaaaccag 1981 ttccggaaga acctgcggga gtcgcagcag ctccgatggg atgagagctg ggtgcagact 2041 gtgctccctt tggttatgga cacagaaaac ttgtactttc aaggctCCAG CTTTCTTGTA 2101 CAAAGTTGGC ATTATAAGAA AGCATTGCTT ATCAATTTGT TGCAACGAAC AGGTCACTAT 2161 CAGTCAAAAT AAAATCATTA TTTGCCATCC AGCTGATATC CCCTATAGTG AGTCGTATTA 2221 CATGGTCATA GCTGTTTCCT GGCAGCTCTG GCCCGTGTCT CAAAATCTCT GATGTTACAT 2281 TGCACAAGAT AAAATAATAT CATCATGAAC AATAAAACTG TCTGCTTACA TAAACAGTAA 2341 TACAAGGGGT GTTATGAGCC ATATTCAACG GGAAACGTCG AGGCCGCGAT TAAATTCCAA 2401 CATGGATGCT GATTTATATG GGTATAAATG GGCTCGCGAT AATGTCGGGC AATCAGGTGC 2461 GACAATCTAT CGCTTGTATG GGAAGCCCGA TGCGCCAGAG TTGTTTCTGA AACATGGCAA 2521 AGGTAGCGTT GCCAATGATG TTACAGATGA GATGGTCAGA CTAAACTGGC TGACGGAATT 2581 TATGCCTCTT CCGACCATCA AGCATTTTAT CCGTACTCCT GATGATGCAT GGTTACTCAC 2641 CACTGCGATC CCCGGAAAAA CAGCATTCCA GGTATTAGAA GAATATCCTG ATTCAGGTGA 2701 AAATATTGTT GATGCGCTGG CAGTGTTCCT GCGCCGGTTG CATTCGATTC CTGTTTGTAA 2761 TTGTCCTTTT AACAGCGATC GCGTATTTCG TCTCGCTCAG GCGCAATCAC GAATGAATAA 2821 CGGTTTGGTT GATGCGAGTG ATTTTGATGA CGAGCGTAAT GGCTGGCCTG TTGAACAAGT 2881 CTGGAAAGAA ATGCATAAAC TTTTGCCATT CTCACCGGAT TCAGTCGTCA CTCATGGTGA 2941 TTTCTCACTT GATAACCTTA TTTTTGACGA GGGGAAATTA ATAGGTTGTA TTGATGTTGG 3001 ACGAGTCGGA ATCGCAGACC GATACCAGGA TCTTGCCATC CTATGGAACT GCCTCGGTGA 3061 GTTTTCTCCT TCATTACAGA AACGGCTTTT TCAAAAATAT GGTATTGATA ATCCTGATAT 3121 GAATAAATTG CAGTTTCATT TGATGCTCGA TGAGTTTTTC TAATCAGAAT TGGTTAATTG 3181 GTTGTAACAC TGGCAGAGCA TTACGCTGAC TTGACGGGAC GGCGCAAGCT CATGACCAAA 3241 ATCCCTTAAC GTGAGTTACG CGTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA 3301 AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC 3361 ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT 3421 AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC CGTAGTTAGG 3481 CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC 3541 AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT 3601 ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA 3661 GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT 3721 TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG 3781 CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA 3841 CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA 3901 CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT //