LOCUS ALG1-pGEc2-DEST 6613 bp DNA circular 22-JAN-2013 FEATURES Location/Qualifiers misc_feature complement(6091..6220) /note="3'-ITR" misc_feature complement(5789..6003) /note="BGH pA" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" CDS 129..989 /dnas_title="AmpR" /label=AmpR /vntifkey="4" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_feature 5241..5782 /note="WPRE" misc_recomb complement(2974..3001) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_feature 4420..4442 /note="attB2" misc_feature 5220..5225 /note="BstB1 site" misc_feature 4443..5138 /note="Super GFP" misc_feature 5139..5189 /note="AviTag" misc_feature 5190..5213 /note="His Tag" PCR_frame 3002..4420 /note="Reading Frame" /set="Set 1" source 3006..4397 /dnas_title="ALG1-pGEc2-DEST" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:1099 IMAGE:3510605" /tissue_type="Brain, neuroblastoma" /clone_lib="NIH_MGC_19" /lab_host="DH10B-R" /note="Vector: pOTB7" gene 3006..4397 /dnas_title="ALG1" /gene="ALG1" /gene_synonym="HMAT1" /gene_synonym="HMT-1" /gene_synonym="HMT1" /db_xref="GeneID:56052" /db_xref="HGNC:18294" /db_xref="MIM:605907" CDS 3006..4397 /dnas_title="asparagine-linked glycosylation 1, beta-1,4-mannosyltransferase homolog (S. cerevisiae)" /gene="ALG1" /gene_synonym="HMAT1" /gene_synonym="HMT-1" /gene_synonym="HMT1" /codon_start=1 /product="asparagine-linked glycosylation 1, beta-1,4-mannosyltransferase homolog (S. cerevisiae)" /protein_id="AAH04402.1" /db_xref="GI:13325168" /db_xref="GeneID:56052" /db_xref="HGNC:18294" /db_xref="MIM:605907" /translation="MAASCLVLLALCLLLPLLLLGGWKRWRRGRAARHVVAVVLGDVG RSPRMQYHALSLAMHGFSVTLLGFCNSKPHDELLQNNRIQIVGLTELQSLAVGPRVFQ YGVKVVLQAMYLLWKLMWREPGAYIFLQNPPGLPSIAVCWFVGCLCGSKLVIDWHNYG YSIMGLVHGPNHPLVLLAKWYEKFFGRLSHLNLCVTNAMREDLADNWHIRAVTVYDKP ASFFKETPLDLQHRLFMKLGSMHSPFRARSEPEDPVTERSAFTERDAGNGLVTRLRER PALLVSSTSWTEDEDFSILLAALEKFEQLTLDGHNLPSLVCVITGKGPLREYYSRLIH QKHFQHIQVCTPWLEAEDYPLLLGSADLGVCLHTSSSGLDLPMKVVDMFGCCLPVCAV NFKCLHELVKHEENGLVFEDSEELAAQLQMLFSNFPDPAGKLNQFRKNLRESQQLRWD ESWVQTVLPLVMDT" misc_feature 3006..4397 /note="ALG1 coding region" misc_difference 3805..3805 /dnas_title="ALG1" /gene="ALG1" /gene_synonym="HMAT1" /gene_synonym="HMT-1" /gene_synonym="HMT1" /note="'A' in cDNA is 'G' in the human genome; amino acid difference: 'N' in cDNA, 'S' in the human genome. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." misc_feature 4398..4418 /note="TEV site" PCR_primer 2992..3001 /dnas_title="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /primer="CAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" PCR_primer complement(3002..4427) /dnas_title="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original="ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG" PCR_product complement(2992..4427) /pair="Pair 1" /set="Set 1" /current=1 /score="1.000" vector join(4428..6613,1..2991) /source="pGEc2-DEST" /type="Destination Vector" clone 2992..4427 /source="ALG1-C-term-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCATCACAA GTTTGTACAA AAAAGCAGGC 3001 TCGCCatggc ggcctcatgc ttggtcctgc tggcgctgtg tctgctgctg ccgctgctgc 3061 tgctgggagg atggaagcgc tggcgccggg ggcgggcggc ccggcatgta gtagcggtgg 3121 tgctgggcga cgtgggccgc agcccccgta tgcagtacca cgcgctgtcg ttggccatgc 3181 acggcttctc ggtgaccctc ctggggttct gcaactccaa accccatgat gagctcttgc 3241 agaacaacag aattcagatt gtggggttga cagaacttca gagtcttgca gttgggcccc 3301 gagttttcca gtacggagtc aaagttgtac ttcaggctat gtacttgctg tggaagttga 3361 tgtggaggga gccaggtgcc tatatctttc tccagaaccc cccaggtctg cctagcattg 3421 ctgtctgctg gttcgtgggc tgcctttgtg gaagcaagct cgtcattgac tggcacaact 3481 atggctactc catcatgggt ctggtgcatg gccccaacca tcccctcgtt ctgctggcca 3541 agtggtacga gaagttcttt gggcgcctgt cccacctgaa cctgtgtgtt accaatgcta 3601 tgcgagaaga cctggcggat aactggcaca tcagggctgt gaccgtctac gacaagcccg 3661 catctttctt taaagagaca cctctggacc tgcagcaccg gctcttcatg aagctgggca 3721 gcatgcactc tccgttcagg gcccgctcag aacctgagga cccagtcacg gagcggtcgg 3781 ccttcacgga gcgggatgct gggaacgggc tggtgacgcg tctccgtgag cggccagccc 3841 tgctggtcag cagcacgagc tggacagagg acgaagactt ctccatcctg ctggcagctt 3901 tagaaaagtt tgaacaactg actcttgatg gacacaacct tccttctctc gtctgtgtga 3961 taacaggcaa agggcctctg agggagtatt atagccgcct catccaccag aagcacttcc 4021 agcacatcca ggtctgcacc ccctggctgg aggccgagga ctaccccctg cttctagggt 4081 cggcggacct gggtgtctgt ctgcacacgt cctccagtgg cctggacctg cccatgaagg 4141 tggtggacat gttcgggtgc tgtttgcctg tgtgtgctgt gaacttcaag tgtttacatg 4201 agctggtgaa acatgaagaa aatggcctgg tctttgagga ctcagaggaa ctggcagctc 4261 agctgcagat gcttttctca aactttcctg atcctgcggg caagctaaac cagttccgga 4321 agaacctgcg ggagtcgcag cagctccgat gggatgagag ctgggtgcag actgtgctcc 4381 ctttggttat ggacacagaa aacttgtact ttcaaggctC CAGCTTTCTT GTACAAAGTG 4441 GTATGAGCAA GGGCGAGGAA CTGTTCACCG GCGTGGTGCC CATCCTGGTG GAACTGGATG 4501 GCGACGTGAA CGGCCACAAG TTCTCTGTGC GGGGAGAGGG CGAAGGCGAC GCCACAAATG 4561 GCAAGCTGAC CCTGAAGTTC ATCTGCACCA CCGGCAAGCT GCCCGTGCCT TGGCCTACCC 4621 TCGTGACCAC ACTGACCTAC GGCGTGCAGT GCTTCAGCAG ATACCCCGAC CATATGAAGC 4681 GGCACGACTT CTTCAAGAGC GCCATGCCCG AGGGCTACGT GCAGGAACGG ACCATCAGCT 4741 TCAAGGACGA CGGCACCTAC AAGACCAGAG CCGAAGTGAA GTTCGAGGGC GACACCCTCG 4801 TGAACCGGAT CGAGCTGAAG GGCATCGATT TCAAAGAGGA CGGCAACATC CTGGGCCACA 4861 AGCTGGAGTA CAACTTCAAC AGCCACAACG TGTACATCAC CGCCGACAAG CAGAAGAACG 4921 GCATCAAGGC CAACTTCAAG ATCCGGCACA ACGTGGAAGA TGGCAGCGTG CAGCTGGCCG 4981 ACCACTACCA GCAGAACACC CCCATCGGAG ATGGCCCCGT GCTGCTGCCC GACAACCACT 5041 ACCTGAGCAC CCAGAGCGTG CTGAGCAAGG ACCCCAACGA GAAGCGGGAC CACATGGTGC 5101 TGCTGGAATT CGTGACCGCC GCTGGCATCA CCCACGGCAT GAGCGGCCTG AACGACATCT 5161 TCGAGGCCCA GAAAATCGAG TGGCACGAGC ACCACCATCA TCACCATCAC CACTGATGAT 5221 TCGAAAAATA ATAAGGATCC AATCAACCTC TGGATTACAA AATTTGTGAA AGATTGACTG 5281 GTATTCTTAA CTATGTTGCT CCTTTTACGC TATGTGGATA CGCTGCTTTA ATGCCTTTGT 5341 ATCATGCTAT TGCTTCCCGT ATGGCTTTCA TTTTCTCCTC CTTGTATAAA TCCTGGTTGC 5401 TGTCTCTTTA TGAGGAGTTG TGGCCCGTTG TCAGGCAACG TGGCGTGGTG TGCACTGTGT 5461 TTGCTGACGC AACCCCCACT GGTTGGGGCA TTGCCACCAC CTGTCAGCTC CTTTCCGGGA 5521 CTTTCGCTTT CCCCCTCCCT ATTGCCACGG CGGAACTCAT CGCCGCCTGC CTTGCCCGCT 5581 GCTGGACAGG GGCTCGGCTG TTGGGCACTG ACAATTCCGT GGTGTTGTCG GGGAAGCTGA 5641 CGTCCTTTCC ATGGCTGCTC GCCTGTGTTG CCACCTGGAT TCTGCGCGGG ACGTCCTTCT 5701 GCTACGTCCC TTCGGCCCTC AATCCAGCGG ACCTTCCTTC CCGCGGCCTG CTGCCGGCTC 5761 TGCGGCCTCT TCCGCGTCTT CGAGATCTGC CTCGACTGTG CCTTCTAGTT GCCAGCCATC 5821 TGTTGTTTGC CCCTCCCCCG TGCCTTCCTT GACCCTGGAA GGTGCCACTC CCACTGTCCT 5881 TTCCTAATAA AATGAGGAAA TTGCATCGCA TTGTCTGAGT AGGTGTCATT CTATTCTGGG 5941 GGGTGGGGTG GGGCAGGACA GCAAGGGGGA GGATTGGGAA GACAATAGCA GGCATGCTGG 6001 GGACTCGAGT TAAGGGCGAA TTGCCGATTA GGATCTTCCT AGAGCATGGC TACGTAGATA 6061 AGTAGCATGG CGGGTTAATC ATTAACTACA AGGAACCCCT AGTGATGGAG TTGGCCACTC 6121 CCTCTCTGCG CGCTCGCTCG CTCACTGAGG CCGGGCGACC AAAGGTCGCC CGACGCCCGG 6181 GCTTTGCCCG GGCGGCCTCA GTGAGCGAGC GAGCGCGCAG CCTTAATTAA GAGCTCATGG 6241 CGCGCCTAGG CCTTGACGGC CTTCCGCCAA TTCGCCCTAT AGTGAGTCGT ATTACGTCGC 6301 GCTCACTGGC CGTCGTTTTA CAACGTCGTG ACTGGGAAAA CCCTGGCGTT ACCCAACTTA 6361 ATCGCCTTGC AGCACATCCC CCTTTCGCCA GCTGGCGTAA TAGCGAAGAG GCCCGCACCG 6421 AAACGCCCTT CCCAACAGTT GCGCAGCCTG AATGGCGAAT GGGAGCGCCC TGTAGCGGCC 6481 ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA 6541 TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTTAACA AAATATTAAC 6601 GCTTACAATT TAG //