LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2021..2117) /label=attL2 /note="attL2 (c)" misc_signal complement(2132..2151) /note="T7 Promoter/priming site (c)" primer_bind 2159..2175 /note="M13 Reverse priming site" gene 2288..3097 /note="Kanamycin resistance gene" rep_origin 3218..3891 /note="pUC origin" vector join(2029..3894,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 669..691 /note="TEV site" source 692..2014 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:1099 IMAGE:3510605" /tissue_type="Brain, neuroblastoma" /clone_lib="NIH_MGC_19" /lab_host="DH10B-R" /note="Vector: pOTB7" gene 692..2014 /gene="ALG1" /gene_synonym=HMAT1 /gene_synonym=HMT-1 /gene_synonym=HMT1 /db_xref="GeneID:56052" /db_xref="HGNC:18294" /db_xref="MIM:605907" CDS 692..2014 /dnas_title="asparagine-linked glycosylation 1, beta-1,4-mannosyltransferase homolog (S. cerevisiae)" /gene="ALG1" /gene_synonym=HMAT1 /gene_synonym=HMT-1 /gene_synonym=HMT1 /codon_start=1 /product="asparagine-linked glycosylation 1, beta-1,4-mannosyltransferase homolog (S. cerevisiae)" /protein_id="AAH04402.1" /db_xref="GI:13325168" /db_xref="GeneID:56052" /db_xref="HGNC:18294" /db_xref="MIM:605907" /translation="MAASCLVLLALCLLLPLLLLGGWKRWRRGRAARHVVAVVLGDVG RSPRMQYHALSLAMHGFSVTLLGFCNSKPHDELLQNNRIQIVGLTELQSLAVGPRVFQ YGVKVVLQAMYLLWKLMWREPGAYIFLQNPPGLPSIAVCWFVGCLCGSKLVIDWHNYG YSIMGLVHGPNHPLVLLAKWYEKFFGRLSHLNLCVTNAMREDLADNWHIRAVTVYDKP ASFFKETPLDLQHRLFMKLGSMHSPFRARSEPEDPVTERSAFTERDAGNGLVTRLRER PALLVSSTSWTEDEDFSILLAALEKFEQLTLDGHNLPSLVCVITGKGPLREYYSRLIH QKHFQHIQVCTPWLEAEDYPLLLGSADLGVCLHTSSSGLDLPMKVVDMFGCCLPVCAV NFKCLHELVKHEENGLVFEDSEELAAQLQMLFSNFPDPAGKLNQFRKNLRESQQLRWD ESWVQTVLPLVMDT" misc_feature 692..2014 /note="ALG1 coding region" misc_difference 1422..1422 /gene="ALG1" /gene_synonym=HMAT1 /gene_synonym=HMT-1 /gene_synonym=HMT1 /note="'A' in cDNA is 'G' in the human genome; amino acid difference: 'N' in cDNA, 'S' in the human genome. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg caagcgctgg cgccgggggc gggcggcccg 721 gcatgtagta gcggtggtgc tgggcgacgt gggccgcagc ccccgtatgc agtaccacgc 781 gctgtcgttg gccatgcacg gcttctcggt gaccctcctg gggttctgca actccaaacc 841 ccatgatgag ctcttgcaga acaacagaat tcagattgtg gggttgacag aacttcagag 901 tcttgcagtt gggccccgag ttttccagta cggagtcaaa gttgtacttc aggctatgta 961 cttgctgtgg aagttgatgt ggagggagcc aggtgcctat atctttctcc agaacccccc 1021 aggtctgcct agcattgctg tctgctggtt cgtgggctgc ctttgtggaa gcaagctcgt 1081 cattgactgg cacaactatg gctactccat catgggtctg gtgcatggcc ccaaccatcc 1141 cctcgttctg ctggccaagt ggtacgagaa gttctttggg cgcctgtccc acctgaacct 1201 gtgtgttacc aatgctatgc gagaagacct ggcggataac tggcacatca gggctgtgac 1261 cgtctacgac aagcccgcat ctttctttaa agagacacct ctggacctgc agcaccggct 1321 cttcatgaag ctgggcagca tgcactctcc gttcagggcc cgctcagaac ctgaggaccc 1381 agtcacggag cggtcggcct tcacggagcg ggatgctggg aacgggctgg tgacgcgtct 1441 ccgtgagcgg ccagccctgc tggtcagcag cacgagctgg acagaggacg aagacttctc 1501 catcctgctg gcagctttag aaaagtttga acaactgact cttgatggac acaaccttcc 1561 ttctctcgtc tgtgtgataa caggcaaagg gcctctgagg gagtattata gccgcctcat 1621 ccaccagaag cacttccagc acatccaggt ctgcaccccc tggctggagg ccgaggacta 1681 ccccctgctt ctagggtcgg cggacctggg tgtctgtctg cacacgtcct ccagtggcct 1741 ggacctgccc atgaaggtgg tggacatgtt cgggtgctgt ttgcctgtgt gtgctgtgaa 1801 cttcaagtgt ttacatgagc tggtgaaaca tgaagaaaat ggcctggtct ttgaggactc 1861 agaggaactg gcagctcagc tgcagatgct tttctcaaac tttcctgatc ctgcgggcaa 1921 gctaaaccag ttccggaaga acctgcggga gtcgcagcag ctccgatggg atgagagctg 1981 ggtgcagact gtgctccctt tggttatgga cacaTAGGAC CCAGCTTTCT TGTACAAAGT 2041 TGGCATTATA AGAAAGCATT GCTTATCAAT TTGTTGCAAC GAACAGGTCA CTATCAGTCA 2101 AAATAAAATC ATTATTTGCC ATCCAGCTGA TATCCCCTAT AGTGAGTCGT ATTACATGGT 2161 CATAGCTGTT TCCTGGCAGC TCTGGCCCGT GTCTCAAAAT CTCTGATGTT ACATTGCACA 2221 AGATAAAATA ATATCATCAT GAACAATAAA ACTGTCTGCT TACATAAACA GTAATACAAG 2281 GGGTGTTATG AGCCATATTC AACGGGAAAC GTCGAGGCCG CGATTAAATT CCAACATGGA 2341 TGCTGATTTA TATGGGTATA AATGGGCTCG CGATAATGTC GGGCAATCAG GTGCGACAAT 2401 CTATCGCTTG TATGGGAAGC CCGATGCGCC AGAGTTGTTT CTGAAACATG GCAAAGGTAG 2461 CGTTGCCAAT GATGTTACAG ATGAGATGGT CAGACTAAAC TGGCTGACGG AATTTATGCC 2521 TCTTCCGACC ATCAAGCATT TTATCCGTAC TCCTGATGAT GCATGGTTAC TCACCACTGC 2581 GATCCCCGGA AAAACAGCAT TCCAGGTATT AGAAGAATAT CCTGATTCAG GTGAAAATAT 2641 TGTTGATGCG CTGGCAGTGT TCCTGCGCCG GTTGCATTCG ATTCCTGTTT GTAATTGTCC 2701 TTTTAACAGC GATCGCGTAT TTCGTCTCGC TCAGGCGCAA TCACGAATGA ATAACGGTTT 2761 GGTTGATGCG AGTGATTTTG ATGACGAGCG TAATGGCTGG CCTGTTGAAC AAGTCTGGAA 2821 AGAAATGCAT AAACTTTTGC CATTCTCACC GGATTCAGTC GTCACTCATG GTGATTTCTC 2881 ACTTGATAAC CTTATTTTTG ACGAGGGGAA ATTAATAGGT TGTATTGATG TTGGACGAGT 2941 CGGAATCGCA GACCGATACC AGGATCTTGC CATCCTATGG AACTGCCTCG GTGAGTTTTC 3001 TCCTTCATTA CAGAAACGGC TTTTTCAAAA ATATGGTATT GATAATCCTG ATATGAATAA 3061 ATTGCAGTTT CATTTGATGC TCGATGAGTT TTTCTAATCA GAATTGGTTA ATTGGTTGTA 3121 ACACTGGCAG AGCATTACGC TGACTTGACG GGACGGCGCA AGCTCATGAC CAAAATCCCT 3181 TAACGTGAGT TACGCGTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC 3241 TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT 3301 ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG 3361 CTTCAGCAGA GCGCAGATAC CAAATACTGT TCTTCTAGTG TAGCCGTAGT TAGGCCACCA 3421 CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC 3481 TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA 3541 TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC 3601 GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA 3661 AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG 3721 GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG 3781 ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG 3841 CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTT //