LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(3224..3320) /label=attL2 /note="attL2 (c)" misc_signal complement(3335..3354) /note="T7 Promoter/priming site (c)" primer_bind 3362..3378 /note="M13 Reverse priming site" gene 3491..4300 /note="Kanamycin resistance gene" rep_origin 4421..5094 /note="pUC origin" PCR_frame 669..3224 /note="Reading Frame" /set="Set 1" PCR_primer 652..668 /primer="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original=ACAAGTTTGTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC PCR_primer complement(669..3244) /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original=ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG PCR_product complement(652..3241) /pair="Pair 1" /set="Set 1" /current=1 /score=1.000 vector join(3232..5097,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 3202..3222 /note="TEV site" source 673..3201 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:9213 IMAGE:3877951" /tissue_type="Lung, large cell carcinoma" /clone_lib="NIH_MGC_68" /lab_host="DH10B" /note="Vector: pCMV-SPORT6" gene 673..3201 /gene="PYGB" /gene_synonym=MGC9213 /db_xref="GeneID:5834" /db_xref="HGNC:9723" /db_xref="MIM:138550" CDS 673..3201 /dnas_title="phosphorylase, glycogen; brain" /gene="PYGB" /gene_synonym=MGC9213 /codon_start=1 /product="phosphorylase, glycogen; brain" /protein_id="AAH17045.1" /db_xref="GI:16877586" /db_xref="GeneID:5834" /db_xref="HGNC:9723" /db_xref="MIM:138550" /translation="MAKPLTDSEKRKQISVRGLAGLGDVAEVRKSFNRHLHFTLVKDR NVATPRDYFFALAHTVRDHLVGRWIRTQQHYYERDPKRIYYLSLEFYMGRTLQNTMVN LGLQNACDEAIYQLGLDLEELEEIEEDAGLGNGGLGRLAACFLDSMATLGLAAYGYGI RYEFGIFNQKIVNGWQVEEADDWLRYGNPWEKARPEYMLPVHFYGRVEHTPDGVKWLD TQVVLAMPYDTPVPGYKNNTVNTMRLWSAKAPNDFKLQDFNVGDYIEAVLDRNLAENI SRVLYPNDNFFEGKELRLKQEYFVVAATLQDIIRRFKSSKFGCRDPVRTCFETFPDKV AIQLNDTHPALSIPELMRILVDVEKVDWDKAWEITKKTCAYTNHTVLPEALERWPVSM FEKLLPRHLEIIYAINQRHLDHVAALFPGDVDRLRRMSVIEEGDCKRINMAHLCVIGS HAVNGVARIHSEIVKQSVFKDFYELEPEKFQNKTNGITPRRWLLLCNPGLADTIVEKI GEEFLTDLSQLKKLLPLVSDEVFIRDVAKVKQENKLKFSAFLEKEYKVKINPSSMFDV HVKRIHEYKRQLLNCLHVVTLYNRIKRDPAKAFVPRTVMIGGKAAPGYHMAKLIIKLV TSIGDVVNHDPVVGDRLKVIFLENYRVSLAEKVIPAADLSQQISTAGTEASGTGNMKF MLNGALTIGTMDGANVEMAEEAGAENLFIFGLRVEDVEALDRKGYNAREYYDHLPELK QAVDQISSGFFSPKEPDCFKDIVNMLMHHDRFKVFADYEAYMQCQAQVDQLYRNPKEW TKKVIRNIACSGKFSSDRTITEYAREIWGVEPSDLQIPPPNIPRD" misc_feature 673..3201 /note="PYGB coding region" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTCG CCatggcgaa gccgctgacg gacagcgaga agcggaagca gatcagcgtg 721 cgcggcctgg cggggctagg cgacgtggcc gaggtgcgga agagcttcaa ccggcacttg 781 cacttcacgc tggtcaagga ccgcaatgtg gccacgcccc gcgactactt cttcgcgctg 841 gcgcacacgg tgcgcgacca cctcgtgggc cgctggatcc gcacgcagca gcactactac 901 gagcgcgacc ccaagcgcat ttattatctt tccctggaat tctacatggg tcgcacgctg 961 cagaacacga tggtgaacct gggccttcag aatgcctgcg atgaagccat ctatcagttg 1021 gggttagact tggaggaact cgaggagata gaagaagatg ctggccttgg gaatggaggc 1081 ctggggaggc tggcagcgtg tttccttgac tcaatggcta ccttgggcct ggcagcatac 1141 ggctatggaa tccgctatga atttgggatt tttaaccaga agattgtcaa tggctggcag 1201 gtagaggagg ccgatgactg gctgcgctac ggcaacccct gggagaaagc gcggcctgag 1261 tatatgcttc ccgtgcactt ctacggacgc gtggagcaca cccccgacgg cgtgaagtgg 1321 ctggacacac aggtggtgct ggccatgccc tacgacaccc cagtgcccgg ctacaagaac 1381 aacaccgtca acaccatgcg gctgtggtcc gccaaggctc ccaacgactt caagctgcag 1441 gacttcaacg tgggagacta catcgaggcg gtcctggacc ggaacttggc tgagaacatc 1501 tccagggtcc tgtatccaaa tgataacttc tttgagggga aggagctgcg gctgaagcag 1561 gagtacttcg tggtggccgc cacgctccag gacatcatcc gccgcttcaa gtcgtccaag 1621 ttcggctgcc gggaccctgt gagaacctgt ttcgagacgt tcccagacaa ggtggccatc 1681 cagctgaacg acacccaccc cgccctctcc atccctgagc tcatgcggat cctggtggac 1741 gtggagaagg tggactggga caaggcctgg gaaatcacga agaagacctg tgcatacacc 1801 aaccacactg tgctgcctga ggccttggag cgctggcccg tgtccatgtt tgagaagctg 1861 ctgccgcggc acctggagat aatctatgcc atcaaccagc ggcacctgga ccacgtggcc 1921 gcgctgtttc ccggcgatgt ggaccgcctg cgcaggatgt ctgtgatcga ggagggggac 1981 tgcaagcgga tcaacatggc ccacctgtgt gtgattgggt cccatgctgt caatggtgtg 2041 gcgaggatcc actcggagat cgtgaaacag tcggtcttta aggattttta tgaactggag 2101 ccagagaagt tccagaataa gaccaatggc atcacccccc gccggtggct gctgctgtgc 2161 aacccggggc tggccgatac catcgtggag aaaattgggg aggagttcct gactgacctg 2221 agccagctga agaagctgct gccgctggtc agtgacgagg tgttcatcag ggacgtggcc 2281 aaggtcaaac aggagaacaa gctcaagttc tcggccttcc tggagaagga gtacaaggtg 2341 aagatcaacc cctcctccat gttcgatgtg catgtgaaga ggatccacga gtacaagcgg 2401 cagctgctca actgcctgca cgtcgtcacc ctgtacaatc gaatcaagag agacccggcc 2461 aaggcttttg tgcccaggac tgttatgatt gggggcaagg cagcgcccgg ttaccacatg 2521 gccaagctga tcatcaagtt ggtcacctcc atcggcgacg tcgtcaatca tgacccagtt 2581 gtgggtgaca ggttgaaagt gatcttcctg gagaactacc gtgtgtcctt ggctgagaaa 2641 gtgatcccgg ccgctgatct gtcgcagcag atctccactg caggcaccga ggcctcaggc 2701 acaggcaaca tgaagttcat gctcaacggg gccctcacca tcggcaccat ggacggcgcc 2761 aacgtggaga tggccgagga ggccggggcc gagaacctct tcatcttcgg cctgcgggtg 2821 gaggatgtcg aggccttgga ccggaaaggg tacaatgcca gggagtacta cgaccacctg 2881 cccgagctga agcaggccgt ggaccagatc agcagtggct ttttttctcc caaggagcca 2941 gactgcttca aggacatcgt gaacatgctg atgcaccatg acaggttcaa ggtgtttgca 3001 gactatgaag cctacatgca gtgccaggca caggtggacc agctgtaccg gaaccccaag 3061 gagtggacca agaaggtcat caggaacatc gcctgctcgg gcaagttctc cagtgaccgg 3121 accatcacgg agtatgcacg ggagatctgg ggtgtggagc cctccgacct gcagatcccg 3181 ccccccaaca tcccccggga cgaaaacttg tactttcaag gctCCAGCTT TCTTGTACAA 3241 AGTTGGCATT ATAAGAAAGC ATTGCTTATC AATTTGTTGC AACGAACAGG TCACTATCAG 3301 TCAAAATAAA ATCATTATTT GCCATCCAGC TGATATCCCC TATAGTGAGT CGTATTACAT 3361 GGTCATAGCT GTTTCCTGGC AGCTCTGGCC CGTGTCTCAA AATCTCTGAT GTTACATTGC 3421 ACAAGATAAA ATAATATCAT CATGAACAAT AAAACTGTCT GCTTACATAA ACAGTAATAC 3481 AAGGGGTGTT ATGAGCCATA TTCAACGGGA AACGTCGAGG CCGCGATTAA ATTCCAACAT 3541 GGATGCTGAT TTATATGGGT ATAAATGGGC TCGCGATAAT GTCGGGCAAT CAGGTGCGAC 3601 AATCTATCGC TTGTATGGGA AGCCCGATGC GCCAGAGTTG TTTCTGAAAC ATGGCAAAGG 3661 TAGCGTTGCC AATGATGTTA CAGATGAGAT GGTCAGACTA AACTGGCTGA CGGAATTTAT 3721 GCCTCTTCCG ACCATCAAGC ATTTTATCCG TACTCCTGAT GATGCATGGT TACTCACCAC 3781 TGCGATCCCC GGAAAAACAG CATTCCAGGT ATTAGAAGAA TATCCTGATT CAGGTGAAAA 3841 TATTGTTGAT GCGCTGGCAG TGTTCCTGCG CCGGTTGCAT TCGATTCCTG TTTGTAATTG 3901 TCCTTTTAAC AGCGATCGCG TATTTCGTCT CGCTCAGGCG CAATCACGAA TGAATAACGG 3961 TTTGGTTGAT GCGAGTGATT TTGATGACGA GCGTAATGGC TGGCCTGTTG AACAAGTCTG 4021 GAAAGAAATG CATAAACTTT TGCCATTCTC ACCGGATTCA GTCGTCACTC ATGGTGATTT 4081 CTCACTTGAT AACCTTATTT TTGACGAGGG GAAATTAATA GGTTGTATTG ATGTTGGACG 4141 AGTCGGAATC GCAGACCGAT ACCAGGATCT TGCCATCCTA TGGAACTGCC TCGGTGAGTT 4201 TTCTCCTTCA TTACAGAAAC GGCTTTTTCA AAAATATGGT ATTGATAATC CTGATATGAA 4261 TAAATTGCAG TTTCATTTGA TGCTCGATGA GTTTTTCTAA TCAGAATTGG TTAATTGGTT 4321 GTAACACTGG CAGAGCATTA CGCTGACTTG ACGGGACGGC GCAAGCTCAT GACCAAAATC 4381 CCTTAACGTG AGTTACGCGT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG 4441 ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC 4501 GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC 4561 TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTTCTTCTA GTGTAGCCGT AGTTAGGCCA 4621 CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT 4681 GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC 4741 GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG 4801 AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC 4861 CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC 4921 GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT 4981 CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC 5041 CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTT //