LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(3233..3329) /label=attL2 /note="attL2 (c)" misc_signal complement(3344..3363) /note="T7 Promoter/priming site (c)" primer_bind 3371..3387 /note="M13 Reverse priming site" gene 3500..4309 /note="Kanamycin resistance gene" rep_origin 4430..5103 /note="pUC origin" PCR_frame 669..3233 /note="Reading Frame" /set="Set 1" PCR_primer 652..668 /primer="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original=ACAAGTTTGTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC PCR_primer complement(669..3253) /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original=ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG PCR_product complement(652..3250) /pair="Pair 1" /set="Set 1" /current=1 /score=1.000 vector join(3241..5106,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 3211..3231 /note="TEV site" source 673..3210 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:2000 IMAGE:2967205" /tissue_type="Skin, melanotic melanoma." /clone_lib="NIH_MGC_20" /lab_host="DH10B-R" /note="Vector: pOTB7" gene 673..3210 /gene="PYGL" /gene_synonym=GSD6 /db_xref="GeneID:5836" /db_xref="HGNC:9725" /db_xref="MIM:232700" CDS 673..3210 /dnas_title="phosphorylase, glycogen, liver" /gene="PYGL" /gene_synonym=GSD6 /codon_start=1 /product="phosphorylase, glycogen, liver" /protein_id="AAH09895.3" /db_xref="GI:112180335" /db_xref="GeneID:5836" /db_xref="HGNC:9725" /db_xref="MIM:232700" /translation="MAKPLTDQEKRRQISIRGIVGVENVAELKKSFNRHLHFTLVKDR NVATTRDYYFALAHTVRDHLVGRWIRTQQHYYDKCPKRVYYLSLEFYMGRTLQNTMIN LGLQNACDEAIYQLGLDIEELEEIEEDAGLGNGGLGRLAACFLDSMATLGLAAYGYGI RYEYGIFNQKIRDGWQVEEADDWLRYGNPWEKSRPEFMLPVHFYGKVEHTNTGTKWID TQVVLALPYDTPVPGYMNNTVNTMRLWSARAPNDFNLRDFNVGDYIQAVLDRNLAENI SRVLYPNDNFFEGKELRLKQEYFVVAATLQDIIRRFKASKFGSTRGAGTVFDAFPDQV AIQLNDTHPALAIPELMRIFVDIEKLPWSKAWELTQKTFAYTNHTVLPEALERWPVDL VEKLLPRHLEIIYEINQKHLDRIVALFPKDVDRLRRMSLIEEEGSKRINMAHLCIVGS HAVNGVAKIHSDIVKTKVFKDFSELEPDKFQNKTNGITPRRWLLLCNPGLAELIAEKI GEDYVKDLSQLTKLHSFLGDDVFLRELAKVKQENKLKFSQFLETEYKVKINPSSMFDV QVKRIHEYKRQLLNCLHVITMYNRIKKDPKKLFVPRTVIIGGKAAPGYHMAKMIIKLI TSVADVVNNDPMVGSKLKVIFLENYRVSLAEKVIPATDLSEQISTAGTEASGTGNMKF LNGALTIGTMDGANVEMAEEAGEENLFIFGMRIDDVAALDKKGYEAKEYYEALPELKL VIDQIDNGFFSPKQPDLFKDIINMLFYHDRFKVFADYEAYVKCQDKVSQLYMNPKAWN TMVLKNIAASGKFSSDRTIKEYAQNIWNVEPSDLKISLSNESNKVNGN" misc_feature 673..3210 /note="PYGL coding region" misc_difference 2718..2719 /gene="PYGL" /gene_synonym=GSD6 /note="3 bases in the human genome, ATG, are not found in cDNA." ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTCG CCatggcgaa gcccctgacg gaccaggaga agcggcggca gatcagcatc 721 cgcggcatcg tgggcgtgga gaacgtggca gagctgaaga agagtttcaa ccggcacctg 781 cacttcacgc tggtcaagga ccgcaacgtg gccaccaccc gcgactacta cttcgcgctg 841 gcgcacacgg tgcgcgacca cctggtgggg cgctggatcc gcacgcagca gcactactac 901 gacaagtgcc ccaagagggt atattacctc tctctggaat tttacatggg ccgaacatta 961 cagaacacca tgatcaacct cggtctgcaa aatgcctgtg atgaggccat ttaccagctt 1021 ggattggata tagaagagtt agaagaaatt gaagaagatg ctggacttgg caatggtggt 1081 cttgggagac ttgctgcctg cttcttggat tccatggcaa ccctgggact tgcagcctat 1141 ggatacggca ttcggtatga atatgggatt ttcaatcaga agatccgaga tggatggcag 1201 gtagaagaag cagatgattg gctcagatat ggaaaccctt gggagaagtc ccgcccagaa 1261 ttcatgctgc ctgtgcactt ctatggaaaa gtagaacaca ccaacaccgg gaccaagtgg 1321 attgacactc aagtggtcct ggctctgcca tatgacaccc ccgtgcccgg ctacatgaat 1381 aacactgtca acaccatgcg cctctggtct gctcgggcac caaatgactt taacctcaga 1441 gactttaatg ttggagacta cattcaggct gtgctggacc gaaacctggc cgagaacatc 1501 tcccgggtcc tctatcccaa tgacaatttt tttgaaggga aggagctaag attgaagcag 1561 gaatactttg tggtggctgc aaccttgcaa gatatcatcc gccgtttcaa agcctccaag 1621 tttggctcca cccgtggtgc aggaactgtg tttgatgcct tcccggatca ggtggccatc 1681 cagctgaatg acactcaccc tgcactcgcg atccctgagc tgatgaggat ttttgtggat 1741 attgaaaaac tgccctggtc caaggcatgg gagctcaccc agaagacctt cgcctacacc 1801 aaccacacag tgctcccgga agccctggag cgctggcccg tggacctggt ggagaagctg 1861 ctccctcgac atttggaaat catttatgag ataaatcaga agcatttaga tagaattgtg 1921 gccttgtttc ctaaagatgt ggaccgtctg agaaggatgt ctctgataga agaggaagga 1981 agcaaaagga tcaacatggc ccatctctgc attgtcggtt cccatgctgt gaatggcgtg 2041 gctaaaatcc actcagacat cgtgaagact aaagtattca aggacttcag tgagctagaa 2101 cctgacaagt ttcagaataa aaccaatggg atcactccaa ggcgctggct cctactctgc 2161 aacccaggac ttgcagagct catagcagag aaaattggag aagactatgt gaaagacctg 2221 agccagctga cgaagctcca cagcttcctg ggtgatgatg tcttcctccg ggaactcgcc 2281 aaggtgaagc aggagaataa gctgaagttt tctcagttcc tggagacgga gtacaaagtg 2341 aagatcaacc catcctccat gtttgatgtc caggtgaaga ggatacatga gtacaagcga 2401 cagctcttga actgtctgca tgtgatcacg atgtacaacc gcattaagaa agaccctaag 2461 aagttattcg tgccaaggac agttatcatt ggtggtaaag ctgccccagg atatcacatg 2521 gccaaaatga tcataaagct gatcacttca gtggcagatg tggtgaacaa tgaccctatg 2581 gttggaagca agttgaaagt catcttcttg gagaactaca gagtatctct tgctgaaaaa 2641 gtcattccag ccacagatct gtcagagcag atttccactg caggcaccga agcctcgggg 2701 acaggcaata tgaagttcct aaatggggcc ctaactatcg ggaccatgga tggggccaat 2761 gtggaaatgg cagaagaagc tggggaagag aacctgttca tctttggcat gaggatagat 2821 gatgtggctg ctttggacaa gaaagggtac gaggcaaaag aatactatga ggcacttcca 2881 gagctgaagc tggtcattga tcaaattgac aatggctttt tttctcccaa gcagcctgac 2941 ctcttcaaag atatcatcaa catgctattt tatcatgaca ggtttaaagt ctttgcagac 3001 tacgaagcct atgtcaagtg tcaagataaa gtgagtcagc tgtacatgaa tccaaaggcc 3061 tggaacacaa tggtactcaa aaacatagct gcctcgggga aattctccag tgaccgaaca 3121 attaaagaat atgcccaaaa catctggaac gtggaacctt cagatctaaa gatttctcta 3181 tccaatgaat ctaacaaagt caatggaaat gaaaacttgt actttcaagg ctCCAGCTTT 3241 CTTGTACAAA GTTGGCATTA TAAGAAAGCA TTGCTTATCA ATTTGTTGCA ACGAACAGGT 3301 CACTATCAGT CAAAATAAAA TCATTATTTG CCATCCAGCT GATATCCCCT ATAGTGAGTC 3361 GTATTACATG GTCATAGCTG TTTCCTGGCA GCTCTGGCCC GTGTCTCAAA ATCTCTGATG 3421 TTACATTGCA CAAGATAAAA TAATATCATC ATGAACAATA AAACTGTCTG CTTACATAAA 3481 CAGTAATACA AGGGGTGTTA TGAGCCATAT TCAACGGGAA ACGTCGAGGC CGCGATTAAA 3541 TTCCAACATG GATGCTGATT TATATGGGTA TAAATGGGCT CGCGATAATG TCGGGCAATC 3601 AGGTGCGACA ATCTATCGCT TGTATGGGAA GCCCGATGCG CCAGAGTTGT TTCTGAAACA 3661 TGGCAAAGGT AGCGTTGCCA ATGATGTTAC AGATGAGATG GTCAGACTAA ACTGGCTGAC 3721 GGAATTTATG CCTCTTCCGA CCATCAAGCA TTTTATCCGT ACTCCTGATG ATGCATGGTT 3781 ACTCACCACT GCGATCCCCG GAAAAACAGC ATTCCAGGTA TTAGAAGAAT ATCCTGATTC 3841 AGGTGAAAAT ATTGTTGATG CGCTGGCAGT GTTCCTGCGC CGGTTGCATT CGATTCCTGT 3901 TTGTAATTGT CCTTTTAACA GCGATCGCGT ATTTCGTCTC GCTCAGGCGC AATCACGAAT 3961 GAATAACGGT TTGGTTGATG CGAGTGATTT TGATGACGAG CGTAATGGCT GGCCTGTTGA 4021 ACAAGTCTGG AAAGAAATGC ATAAACTTTT GCCATTCTCA CCGGATTCAG TCGTCACTCA 4081 TGGTGATTTC TCACTTGATA ACCTTATTTT TGACGAGGGG AAATTAATAG GTTGTATTGA 4141 TGTTGGACGA GTCGGAATCG CAGACCGATA CCAGGATCTT GCCATCCTAT GGAACTGCCT 4201 CGGTGAGTTT TCTCCTTCAT TACAGAAACG GCTTTTTCAA AAATATGGTA TTGATAATCC 4261 TGATATGAAT AAATTGCAGT TTCATTTGAT GCTCGATGAG TTTTTCTAAT CAGAATTGGT 4321 TAATTGGTTG TAACACTGGC AGAGCATTAC GCTGACTTGA CGGGACGGCG CAAGCTCATG 4381 ACCAAAATCC CTTAACGTGA GTTACGCGTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA 4441 GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA 4501 AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC 4561 GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTTCTTCTAG TGTAGCCGTA 4621 GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT 4681 GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG 4741 ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG 4801 CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC 4861 CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG 4921 AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT 4981 TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG 5041 GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA 5101 CATGTT //