LOCUS PYGL-pGEc2-DEST 7759 bp DNA circular 22-JAN-2013 FEATURES Location/Qualifiers misc_feature complement(7237..7366) /note="3'-ITR" misc_feature complement(6935..7149) /note="BGH pA" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" CDS 129..989 /dnas_title="AmpR" /label=AmpR /vntifkey="4" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_feature 6387..6928 /note="WPRE" misc_recomb complement(2974..3001) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_feature 5566..5588 /note="attB2" misc_feature 6366..6371 /note="BstB1 site" misc_feature 5589..6284 /note="Super GFP" misc_feature 6285..6335 /note="AviTag" misc_feature 6336..6359 /note="His Tag" PCR_frame 3002..5566 /note="Reading Frame" /set="Set 1" source 3006..5543 /dnas_title="PYGL-pGEc2-DEST" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:2000 IMAGE:2967205" /tissue_type="Skin, melanotic melanoma." /clone_lib="NIH_MGC_20" /lab_host="DH10B-R" /note="Vector: pOTB7" gene 3006..5543 /dnas_title="PYGL" /gene="PYGL" /gene_synonym="GSD6" /db_xref="GeneID:5836" /db_xref="HGNC:9725" /db_xref="MIM:232700" CDS 3006..5543 /dnas_title="phosphorylase, glycogen, liver" /gene="PYGL" /gene_synonym="GSD6" /codon_start=1 /product="phosphorylase, glycogen, liver" /protein_id="AAH09895.3" /db_xref="GI:112180335" /db_xref="GeneID:5836" /db_xref="HGNC:9725" /db_xref="MIM:232700" /translation="MAKPLTDQEKRRQISIRGIVGVENVAELKKSFNRHLHFTLVKDR NVATTRDYYFALAHTVRDHLVGRWIRTQQHYYDKCPKRVYYLSLEFYMGRTLQNTMIN LGLQNACDEAIYQLGLDIEELEEIEEDAGLGNGGLGRLAACFLDSMATLGLAAYGYGI RYEYGIFNQKIRDGWQVEEADDWLRYGNPWEKSRPEFMLPVHFYGKVEHTNTGTKWID TQVVLALPYDTPVPGYMNNTVNTMRLWSARAPNDFNLRDFNVGDYIQAVLDRNLAENI SRVLYPNDNFFEGKELRLKQEYFVVAATLQDIIRRFKASKFGSTRGAGTVFDAFPDQV AIQLNDTHPALAIPELMRIFVDIEKLPWSKAWELTQKTFAYTNHTVLPEALERWPVDL VEKLLPRHLEIIYEINQKHLDRIVALFPKDVDRLRRMSLIEEEGSKRINMAHLCIVGS HAVNGVAKIHSDIVKTKVFKDFSELEPDKFQNKTNGITPRRWLLLCNPGLAELIAEKI GEDYVKDLSQLTKLHSFLGDDVFLRELAKVKQENKLKFSQFLETEYKVKINPSSMFDV QVKRIHEYKRQLLNCLHVITMYNRIKKDPKKLFVPRTVIIGGKAAPGYHMAKMIIKLI TSVADVVNNDPMVGSKLKVIFLENYRVSLAEKVIPATDLSEQISTAGTEASGTGNMKF LNGALTIGTMDGANVEMAEEAGEENLFIFGMRIDDVAALDKKGYEAKEYYEALPELKL VIDQIDNGFFSPKQPDLFKDIINMLFYHDRFKVFADYEAYVKCQDKVSQLYMNPKAWN TMVLKNIAASGKFSSDRTIKEYAQNIWNVEPSDLKISLSNESNKVNGN" misc_feature 3006..5543 /note="PYGL coding region" misc_difference 5051..5052 /dnas_title="PYGL" /gene="PYGL" /gene_synonym="GSD6" /note="3 bases in the human genome, ATG, are not found in cDNA." misc_feature 5544..5564 /note="TEV site" PCR_primer 2992..3001 /dnas_title="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /primer="CAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" PCR_primer complement(3002..5573) /dnas_title="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original="ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG" PCR_product complement(2992..5573) /pair="Pair 1" /set="Set 1" /current=1 /score="1.000" vector join(5574..7759,1..2991) /source="pGEc2-DEST" /type="Destination Vector" clone 2992..5573 /source="PYGL-C-term-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCATCACAA GTTTGTACAA AAAAGCAGGC 3001 TCGCCatggc gaagcccctg acggaccagg agaagcggcg gcagatcagc atccgcggca 3061 tcgtgggcgt ggagaacgtg gcagagctga agaagagttt caaccggcac ctgcacttca 3121 cgctggtcaa ggaccgcaac gtggccacca cccgcgacta ctacttcgcg ctggcgcaca 3181 cggtgcgcga ccacctggtg gggcgctgga tccgcacgca gcagcactac tacgacaagt 3241 gccccaagag ggtatattac ctctctctgg aattttacat gggccgaaca ttacagaaca 3301 ccatgatcaa cctcggtctg caaaatgcct gtgatgaggc catttaccag cttggattgg 3361 atatagaaga gttagaagaa attgaagaag atgctggact tggcaatggt ggtcttggga 3421 gacttgctgc ctgcttcttg gattccatgg caaccctggg acttgcagcc tatggatacg 3481 gcattcggta tgaatatggg attttcaatc agaagatccg agatggatgg caggtagaag 3541 aagcagatga ttggctcaga tatggaaacc cttgggagaa gtcccgccca gaattcatgc 3601 tgcctgtgca cttctatgga aaagtagaac acaccaacac cgggaccaag tggattgaca 3661 ctcaagtggt cctggctctg ccatatgaca cccccgtgcc cggctacatg aataacactg 3721 tcaacaccat gcgcctctgg tctgctcggg caccaaatga ctttaacctc agagacttta 3781 atgttggaga ctacattcag gctgtgctgg accgaaacct ggccgagaac atctcccggg 3841 tcctctatcc caatgacaat ttttttgaag ggaaggagct aagattgaag caggaatact 3901 ttgtggtggc tgcaaccttg caagatatca tccgccgttt caaagcctcc aagtttggct 3961 ccacccgtgg tgcaggaact gtgtttgatg ccttcccgga tcaggtggcc atccagctga 4021 atgacactca ccctgcactc gcgatccctg agctgatgag gatttttgtg gatattgaaa 4081 aactgccctg gtccaaggca tgggagctca cccagaagac cttcgcctac accaaccaca 4141 cagtgctccc ggaagccctg gagcgctggc ccgtggacct ggtggagaag ctgctccctc 4201 gacatttgga aatcatttat gagataaatc agaagcattt agatagaatt gtggccttgt 4261 ttcctaaaga tgtggaccgt ctgagaagga tgtctctgat agaagaggaa ggaagcaaaa 4321 ggatcaacat ggcccatctc tgcattgtcg gttcccatgc tgtgaatggc gtggctaaaa 4381 tccactcaga catcgtgaag actaaagtat tcaaggactt cagtgagcta gaacctgaca 4441 agtttcagaa taaaaccaat gggatcactc caaggcgctg gctcctactc tgcaacccag 4501 gacttgcaga gctcatagca gagaaaattg gagaagacta tgtgaaagac ctgagccagc 4561 tgacgaagct ccacagcttc ctgggtgatg atgtcttcct ccgggaactc gccaaggtga 4621 agcaggagaa taagctgaag ttttctcagt tcctggagac ggagtacaaa gtgaagatca 4681 acccatcctc catgtttgat gtccaggtga agaggataca tgagtacaag cgacagctct 4741 tgaactgtct gcatgtgatc acgatgtaca accgcattaa gaaagaccct aagaagttat 4801 tcgtgccaag gacagttatc attggtggta aagctgcccc aggatatcac atggccaaaa 4861 tgatcataaa gctgatcact tcagtggcag atgtggtgaa caatgaccct atggttggaa 4921 gcaagttgaa agtcatcttc ttggagaact acagagtatc tcttgctgaa aaagtcattc 4981 cagccacaga tctgtcagag cagatttcca ctgcaggcac cgaagcctcg gggacaggca 5041 atatgaagtt cctaaatggg gccctaacta tcgggaccat ggatggggcc aatgtggaaa 5101 tggcagaaga agctggggaa gagaacctgt tcatctttgg catgaggata gatgatgtgg 5161 ctgctttgga caagaaaggg tacgaggcaa aagaatacta tgaggcactt ccagagctga 5221 agctggtcat tgatcaaatt gacaatggct ttttttctcc caagcagcct gacctcttca 5281 aagatatcat caacatgcta ttttatcatg acaggtttaa agtctttgca gactacgaag 5341 cctatgtcaa gtgtcaagat aaagtgagtc agctgtacat gaatccaaag gcctggaaca 5401 caatggtact caaaaacata gctgcctcgg ggaaattctc cagtgaccga acaattaaag 5461 aatatgccca aaacatctgg aacgtggaac cttcagatct aaagatttct ctatccaatg 5521 aatctaacaa agtcaatgga aatgaaaact tgtactttca aggctCCAGC TTTCTTGTAC 5581 AAAGTGGTAT GAGCAAGGGC GAGGAACTGT TCACCGGCGT GGTGCCCATC CTGGTGGAAC 5641 TGGATGGCGA CGTGAACGGC CACAAGTTCT CTGTGCGGGG AGAGGGCGAA GGCGACGCCA 5701 CAAATGGCAA GCTGACCCTG AAGTTCATCT GCACCACCGG CAAGCTGCCC GTGCCTTGGC 5761 CTACCCTCGT GACCACACTG ACCTACGGCG TGCAGTGCTT CAGCAGATAC CCCGACCATA 5821 TGAAGCGGCA CGACTTCTTC AAGAGCGCCA TGCCCGAGGG CTACGTGCAG GAACGGACCA 5881 TCAGCTTCAA GGACGACGGC ACCTACAAGA CCAGAGCCGA AGTGAAGTTC GAGGGCGACA 5941 CCCTCGTGAA CCGGATCGAG CTGAAGGGCA TCGATTTCAA AGAGGACGGC AACATCCTGG 6001 GCCACAAGCT GGAGTACAAC TTCAACAGCC ACAACGTGTA CATCACCGCC GACAAGCAGA 6061 AGAACGGCAT CAAGGCCAAC TTCAAGATCC GGCACAACGT GGAAGATGGC AGCGTGCAGC 6121 TGGCCGACCA CTACCAGCAG AACACCCCCA TCGGAGATGG CCCCGTGCTG CTGCCCGACA 6181 ACCACTACCT GAGCACCCAG AGCGTGCTGA GCAAGGACCC CAACGAGAAG CGGGACCACA 6241 TGGTGCTGCT GGAATTCGTG ACCGCCGCTG GCATCACCCA CGGCATGAGC GGCCTGAACG 6301 ACATCTTCGA GGCCCAGAAA ATCGAGTGGC ACGAGCACCA CCATCATCAC CATCACCACT 6361 GATGATTCGA AAAATAATAA GGATCCAATC AACCTCTGGA TTACAAAATT TGTGAAAGAT 6421 TGACTGGTAT TCTTAACTAT GTTGCTCCTT TTACGCTATG TGGATACGCT GCTTTAATGC 6481 CTTTGTATCA TGCTATTGCT TCCCGTATGG CTTTCATTTT CTCCTCCTTG TATAAATCCT 6541 GGTTGCTGTC TCTTTATGAG GAGTTGTGGC CCGTTGTCAG GCAACGTGGC GTGGTGTGCA 6601 CTGTGTTTGC TGACGCAACC CCCACTGGTT GGGGCATTGC CACCACCTGT CAGCTCCTTT 6661 CCGGGACTTT CGCTTTCCCC CTCCCTATTG CCACGGCGGA ACTCATCGCC GCCTGCCTTG 6721 CCCGCTGCTG GACAGGGGCT CGGCTGTTGG GCACTGACAA TTCCGTGGTG TTGTCGGGGA 6781 AGCTGACGTC CTTTCCATGG CTGCTCGCCT GTGTTGCCAC CTGGATTCTG CGCGGGACGT 6841 CCTTCTGCTA CGTCCCTTCG GCCCTCAATC CAGCGGACCT TCCTTCCCGC GGCCTGCTGC 6901 CGGCTCTGCG GCCTCTTCCG CGTCTTCGAG ATCTGCCTCG ACTGTGCCTT CTAGTTGCCA 6961 GCCATCTGTT GTTTGCCCCT CCCCCGTGCC TTCCTTGACC CTGGAAGGTG CCACTCCCAC 7021 TGTCCTTTCC TAATAAAATG AGGAAATTGC ATCGCATTGT CTGAGTAGGT GTCATTCTAT 7081 TCTGGGGGGT GGGGTGGGGC AGGACAGCAA GGGGGAGGAT TGGGAAGACA ATAGCAGGCA 7141 TGCTGGGGAC TCGAGTTAAG GGCGAATTGC CGATTAGGAT CTTCCTAGAG CATGGCTACG 7201 TAGATAAGTA GCATGGCGGG TTAATCATTA ACTACAAGGA ACCCCTAGTG ATGGAGTTGG 7261 CCACTCCCTC TCTGCGCGCT CGCTCGCTCA CTGAGGCCGG GCGACCAAAG GTCGCCCGAC 7321 GCCCGGGCTT TGCCCGGGCG GCCTCAGTGA GCGAGCGAGC GCGCAGCCTT AATTAAGAGC 7381 TCATGGCGCG CCTAGGCCTT GACGGCCTTC CGCCAATTCG CCCTATAGTG AGTCGTATTA 7441 CGTCGCGCTC ACTGGCCGTC GTTTTACAAC GTCGTGACTG GGAAAACCCT GGCGTTACCC 7501 AACTTAATCG CCTTGCAGCA CATCCCCCTT TCGCCAGCTG GCGTAATAGC GAAGAGGCCC 7561 GCACCGAAAC GCCCTTCCCA ACAGTTGCGC AGCCTGAATG GCGAATGGGA GCGCCCTGTA 7621 GCGGCCACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC 7681 GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT 7741 ATTAACGCTT ACAATTTAG //