LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2945..3041) /label=attL2 /note="attL2 (c)" misc_signal complement(3056..3075) /note="T7 Promoter/priming site (c)" primer_bind 3083..3099 /note="M13 Reverse priming site" gene 3212..4021 /note="Kanamycin resistance gene" rep_origin 4142..4815 /note="pUC origin" PCR_frame 669..2945 /note="Reading Frame" /set="Set 1" PCR_primer 652..668 /primer="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original=ACAAGTTTGTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC PCR_primer complement(669..2965) /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original=ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG PCR_product complement(652..2962) /pair="Pair 1" /set="Set 1" /current=1 /score=1.000 vector join(2953..4818,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 2923..2943 /note="TEV site" source 673..2922 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:34379 IMAGE:5169145" /tissue_type="Brain, adult medulla" /clone_lib="NIH_MGC_119" /lab_host="DH10B" /note="Vector: pCMV-SPORT6" gene 673..2922 /gene="POMT2" /db_xref="GeneID:29954" /db_xref="HGNC:19743" /db_xref="MIM:607439" CDS 673..2922 /dnas_title="protein-O-mannosyltransferase 2" /gene="POMT2" /codon_start=1 /product="protein-O-mannosyltransferase 2" /protein_id="AAH31651.1" /db_xref="GI:21619461" /db_xref="GeneID:29954" /db_xref="HGNC:19743" /db_xref="MIM:607439" /translation="MPPATGGGLAESELRPRRGRCGPQAARAAGRDVAAEAVARSPKR PAWGSRRFEAVGWWALLALVTLLSFATRFHRLDEPPHICWDETHFGKMGSYYINRTFF FDVHPPLGKMLIGLAGYLSGYDGTFLFQKPGDKYEHHSYMGMRGFCAFLGSWLVPFAY LTVLDLSKSLSAALLTAALLTFDTGCLTLSQYILLDPILMFFIMAAMLSMVKYNSCAD RPFSAPWWFWLSLTGVSLAGALGVKFVGLFIILQVGLNTIADLWYLFGDLSLSLVTVG KHLTARVLCLIVLPLALYTATFAVHFMVLSKSGPGDGFFSSAFQARLSGNNLHNASIP EHLAYGSVITVKNLRMAIGYLHSHRHLYPEGIGARQQQVTTYLHKDYNNLWIIKKHNT NSDPLDPSFPVEFVRHGDIIRLEHKETSRNLHSHYHEAPMTRKHYQVTGYGINGTGDS NDFWRIEVVNRKFGNRIKVLRSRIRFIHLVTGCVLGSSGKVLPKWGWEQLEVTCTPYL KETLNSIWNVEDHINPKLPNISLDVLQPSFPEILLESHMVMIRGNSGLKPKDNEFTSK PWHWPINYQGLRFSGVNDTDFRVYLLGNPVVWWLNLLSIALYLLSGSIIAVAMQRGAR LPAEVAGLSQVLLRGGGQVLLGWTLHYFPFFLMGRVLYFHHYFPAMLFSSMLTGILWD TLLRLCAWGLASWPLARGIHVAGILSLLLGTAYSFYLFHPLAYGMVGPLAQDPQSPMA GLRWLDSWDF" misc_difference 834..834 /gene="POMT2" /note="'T' in cDNA is 'G' in the human genome; no amino acid change. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." misc_feature 673..2922 /note="POMT2 coding region" misc_difference 2055..2055 /gene="POMT2" /note="'A' in cDNA is 'G' in the human genome; no amino acid change. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." misc_difference 2583..2583 /gene="POMT2" /note="'G' in cDNA is 'T' in the human genome; no amino acid change." ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTCG CCatgccgcc ggccacgggc ggaggcctgg cagagtccga gctgcgtccc 721 cggaggggcc gctgtggccc ccaggctgct agggccgcag gccgggacgt ggccgctgag 781 gctgtggcgc gaagccccaa acggcctgct tggggctcac ggcgcttcga ggctgtcggc 841 tggtgggccc tgctggcctt ggtgacgctg ctgtccttcg ccacccgctt ccaccgcttg 901 gacgagccgc cgcacatctg ttgggatgag actcactttg gaaaaatggg aagttactat 961 atcaaccgta catttttctt tgatgtgcac ccgcccctgg gaaagatgct gataggtctt 1021 gctggctacc tgagtggata tgatggtacc tttttgttcc agaagcctgg ggataaatat 1081 gagcatcaca gctacatggg aatgagagga ttctgtgcat tccttggctc ctggctggtc 1141 ccctttgcct acctcactgt actggatctg tccaagtccc tctcggcagc actgctcaca 1201 gctgccctcc tcacctttga cacgggatgc ctcactctgt cccagtacat cctccttgac 1261 cccatcctga tgttcttcat catggctgcc atgctgagca tggtcaagta caactcttgc 1321 gccgacaggc ccttctctgc cccctggtgg ttctggctca gcctgactgg cgttagtctt 1381 gctggtgctt taggggtcaa gtttgttggc ctctttatca tccttcaagt ggggctgaac 1441 accattgcag acctttggta cctgttcgga gacctcagtc tttcattggt gactgtggga 1501 aaacacctga ctgctcgtgt cctgtgcctc atagtgctgc ccctggctct ctatacagcc 1561 acctttgctg ttcacttcat ggtgctgagt aaaagtggcc ctggtgacgg tttcttcagt 1621 tctgccttcc aggcccggct ttcagggaac aacctgcaca atgcttccat ccctgaacac 1681 ctggcctacg gctctgtgat cactgtgaag aacctccgga tggccatcgg ctatctgcac 1741 tcccacaggc acctctaccc cgagggcatt ggtgcccgtc agcagcaggt caccacctat 1801 ttgcacaagg actacaacaa cctgtggatt atcaagaaac ataacacaaa ctcagatccc 1861 ctagaccctt ccttcccagt ggagtttgta agacatggag acattattcg actagaacac 1921 aaagaaactt cccggaactt gcacagtcac tatcatgagg cccccatgac ccggaagcac 1981 tatcaggtca ccggctatgg cataaatgga acaggggact caaatgattt ctggcggatt 2041 gaggtcgtaa acagaaaatt tggaaaccgg atcaaagtgc tgagaagtcg aattcgcttc 2101 atccatttgg tcacaggttg tgtcctgggc tcctcgggaa aggttctgcc caagtggggc 2161 tgggagcagt tggaagttac ttgcacccca tacctgaaag aaaccctcaa ctccatctgg 2221 aatgtggagg accatatcaa tcccaagttg ccaaacatca gcctggatgt gctacagccc 2281 agttttcctg agatcttgct ggaatcccac atggtcatga tccgggggaa cagtggcctc 2341 aaacccaagg acaatgagtt cacgtccaaa ccctggcact ggcctatcaa ctatcagggc 2401 ctacgcttct caggggtcaa tgacacagat ttccgagtct atctgcttgg caacccggtg 2461 gtttggtggc tgaatctgtt gagcatcgcc ctctacctcc tctcagggag catcattgct 2521 gtagccatgc agagaggggc acggctgcca gcggaggttg cagggttgtc ccaggtcctg 2581 ctgcgaggag gcggccaggt cctgctcggc tggacactcc attacttccc gtttttcctg 2641 atgggccggg tcctctactt ccaccactac ttcccagcca tgctcttctc aagcatgttg 2701 acaggcattc tgtgggacac cctcctgcgg ctctgtgcct ggggcttggc ctcatggccc 2761 ctggcgaggg gcatacatgt ggcgggaatc ctgagcctgc tcctgggaac tgcctacagc 2821 ttctacctct tccaccctct ggcttacggg atggttggtc ccctggccca ggacccccaa 2881 agtccaatgg caggactaag gtggctggac tcatgggact ttgaaaactt gtactttcaa 2941 ggctCCAGCT TTCTTGTACA AAGTTGGCAT TATAAGAAAG CATTGCTTAT CAATTTGTTG 3001 CAACGAACAG GTCACTATCA GTCAAAATAA AATCATTATT TGCCATCCAG CTGATATCCC 3061 CTATAGTGAG TCGTATTACA TGGTCATAGC TGTTTCCTGG CAGCTCTGGC CCGTGTCTCA 3121 AAATCTCTGA TGTTACATTG CACAAGATAA AATAATATCA TCATGAACAA TAAAACTGTC 3181 TGCTTACATA AACAGTAATA CAAGGGGTGT TATGAGCCAT ATTCAACGGG AAACGTCGAG 3241 GCCGCGATTA AATTCCAACA TGGATGCTGA TTTATATGGG TATAAATGGG CTCGCGATAA 3301 TGTCGGGCAA TCAGGTGCGA CAATCTATCG CTTGTATGGG AAGCCCGATG CGCCAGAGTT 3361 GTTTCTGAAA CATGGCAAAG GTAGCGTTGC CAATGATGTT ACAGATGAGA TGGTCAGACT 3421 AAACTGGCTG ACGGAATTTA TGCCTCTTCC GACCATCAAG CATTTTATCC GTACTCCTGA 3481 TGATGCATGG TTACTCACCA CTGCGATCCC CGGAAAAACA GCATTCCAGG TATTAGAAGA 3541 ATATCCTGAT TCAGGTGAAA ATATTGTTGA TGCGCTGGCA GTGTTCCTGC GCCGGTTGCA 3601 TTCGATTCCT GTTTGTAATT GTCCTTTTAA CAGCGATCGC GTATTTCGTC TCGCTCAGGC 3661 GCAATCACGA ATGAATAACG GTTTGGTTGA TGCGAGTGAT TTTGATGACG AGCGTAATGG 3721 CTGGCCTGTT GAACAAGTCT GGAAAGAAAT GCATAAACTT TTGCCATTCT CACCGGATTC 3781 AGTCGTCACT CATGGTGATT TCTCACTTGA TAACCTTATT TTTGACGAGG GGAAATTAAT 3841 AGGTTGTATT GATGTTGGAC GAGTCGGAAT CGCAGACCGA TACCAGGATC TTGCCATCCT 3901 ATGGAACTGC CTCGGTGAGT TTTCTCCTTC ATTACAGAAA CGGCTTTTTC AAAAATATGG 3961 TATTGATAAT CCTGATATGA ATAAATTGCA GTTTCATTTG ATGCTCGATG AGTTTTTCTA 4021 ATCAGAATTG GTTAATTGGT TGTAACACTG GCAGAGCATT ACGCTGACTT GACGGGACGG 4081 CGCAAGCTCA TGACCAAAAT CCCTTAACGT GAGTTACGCG TCGTTCCACT GAGCGTCAGA 4141 CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG 4201 CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC 4261 AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTTCTTCT 4321 AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC 4381 TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT 4441 GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG 4501 CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT 4561 ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG 4621 GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG 4681 TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG 4741 GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG 4801 GCCTTTTGCT CACATGTT //