LOCUS POMT2-pGEc1-DEST 6745 bp DNA circular 22-JAN-2013 FEATURES Location/Qualifiers misc_feature complement(6223..6352) /note="3'-ITR" misc_feature complement(5921..6135) /note="BGH pA" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" CDS 129..989 /dnas_title="AmpR" /label=AmpR /vntifkey="4" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_feature 5373..5914 /note="WPRE" misc_recomb complement(2974..3001) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_recomb 5278..5300 /label=attB2 /note="attB2 site" misc_feature 5310..5333 /note="8xHis Tag" misc_feature 5337..5360 /note="StrepTag" PCR_frame 3002..5278 /note="Reading Frame" /set="Set 1" source 3006..5255 /dnas_title="POMT2-pGEc1-DEST" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:34379 IMAGE:5169145" /tissue_type="Brain, adult medulla" /clone_lib="NIH_MGC_119" /lab_host="DH10B" /note="Vector: pCMV-SPORT6" gene 3006..5255 /dnas_title="POMT2" /gene="POMT2" /db_xref="GeneID:29954" /db_xref="HGNC:19743" /db_xref="MIM:607439" CDS 3006..5255 /dnas_title="protein-O-mannosyltransferase 2" /gene="POMT2" /codon_start=1 /product="protein-O-mannosyltransferase 2" /protein_id="AAH31651.1" /db_xref="GI:21619461" /db_xref="GeneID:29954" /db_xref="HGNC:19743" /db_xref="MIM:607439" /translation="MPPATGGGLAESELRPRRGRCGPQAARAAGRDVAAEAVARSPKR PAWGSRRFEAVGWWALLALVTLLSFATRFHRLDEPPHICWDETHFGKMGSYYINRTFF FDVHPPLGKMLIGLAGYLSGYDGTFLFQKPGDKYEHHSYMGMRGFCAFLGSWLVPFAY LTVLDLSKSLSAALLTAALLTFDTGCLTLSQYILLDPILMFFIMAAMLSMVKYNSCAD RPFSAPWWFWLSLTGVSLAGALGVKFVGLFIILQVGLNTIADLWYLFGDLSLSLVTVG KHLTARVLCLIVLPLALYTATFAVHFMVLSKSGPGDGFFSSAFQARLSGNNLHNASIP EHLAYGSVITVKNLRMAIGYLHSHRHLYPEGIGARQQQVTTYLHKDYNNLWIIKKHNT NSDPLDPSFPVEFVRHGDIIRLEHKETSRNLHSHYHEAPMTRKHYQVTGYGINGTGDS NDFWRIEVVNRKFGNRIKVLRSRIRFIHLVTGCVLGSSGKVLPKWGWEQLEVTCTPYL KETLNSIWNVEDHINPKLPNISLDVLQPSFPEILLESHMVMIRGNSGLKPKDNEFTSK PWHWPINYQGLRFSGVNDTDFRVYLLGNPVVWWLNLLSIALYLLSGSIIAVAMQRGAR LPAEVAGLSQVLLRGGGQVLLGWTLHYFPFFLMGRVLYFHHYFPAMLFSSMLTGILWD TLLRLCAWGLASWPLARGIHVAGILSLLLGTAYSFYLFHPLAYGMVGPLAQDPQSPMA GLRWLDSWDF" misc_difference 3167..3167 /dnas_title="POMT2" /gene="POMT2" /note="'T' in cDNA is 'G' in the human genome; no amino acid change. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." misc_feature 3006..5255 /note="POMT2 coding region" misc_difference 4388..4388 /dnas_title="POMT2" /gene="POMT2" /note="'A' in cDNA is 'G' in the human genome; no amino acid change. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." misc_feature 5256..5276 /note="TEV site" misc_difference 4916..4916 /dnas_title="POMT2" /gene="POMT2" /note="'G' in cDNA is 'T' in the human genome; no amino acid change." PCR_primer 2992..3001 /dnas_title="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /primer="CAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" PCR_primer complement(3002..5285) /dnas_title="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original="ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG" PCR_product complement(2992..5285) /pair="Pair 1" /set="Set 1" /current=1 /score="1.000" vector join(5286..6745,1..2991) /source="pGEc1-DEST" /type="Destination Vector" clone 2992..5285 /source="POMT2-C-term-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCATCACAA GTTTGTACAA AAAAGCAGGC 3001 TCGCCatgcc gccggccacg ggcggaggcc tggcagagtc cgagctgcgt ccccggaggg 3061 gccgctgtgg cccccaggct gctagggccg caggccggga cgtggccgct gaggctgtgg 3121 cgcgaagccc caaacggcct gcttggggct cacggcgctt cgaggctgtc ggctggtggg 3181 ccctgctggc cttggtgacg ctgctgtcct tcgccacccg cttccaccgc ttggacgagc 3241 cgccgcacat ctgttgggat gagactcact ttggaaaaat gggaagttac tatatcaacc 3301 gtacattttt ctttgatgtg cacccgcccc tgggaaagat gctgataggt cttgctggct 3361 acctgagtgg atatgatggt acctttttgt tccagaagcc tggggataaa tatgagcatc 3421 acagctacat gggaatgaga ggattctgtg cattccttgg ctcctggctg gtcccctttg 3481 cctacctcac tgtactggat ctgtccaagt ccctctcggc agcactgctc acagctgccc 3541 tcctcacctt tgacacggga tgcctcactc tgtcccagta catcctcctt gaccccatcc 3601 tgatgttctt catcatggct gccatgctga gcatggtcaa gtacaactct tgcgccgaca 3661 ggcccttctc tgccccctgg tggttctggc tcagcctgac tggcgttagt cttgctggtg 3721 ctttaggggt caagtttgtt ggcctcttta tcatccttca agtggggctg aacaccattg 3781 cagacctttg gtacctgttc ggagacctca gtctttcatt ggtgactgtg ggaaaacacc 3841 tgactgctcg tgtcctgtgc ctcatagtgc tgcccctggc tctctataca gccacctttg 3901 ctgttcactt catggtgctg agtaaaagtg gccctggtga cggtttcttc agttctgcct 3961 tccaggcccg gctttcaggg aacaacctgc acaatgcttc catccctgaa cacctggcct 4021 acggctctgt gatcactgtg aagaacctcc ggatggccat cggctatctg cactcccaca 4081 ggcacctcta ccccgagggc attggtgccc gtcagcagca ggtcaccacc tatttgcaca 4141 aggactacaa caacctgtgg attatcaaga aacataacac aaactcagat cccctagacc 4201 cttccttccc agtggagttt gtaagacatg gagacattat tcgactagaa cacaaagaaa 4261 cttcccggaa cttgcacagt cactatcatg aggcccccat gacccggaag cactatcagg 4321 tcaccggcta tggcataaat ggaacagggg actcaaatga tttctggcgg attgaggtcg 4381 taaacagaaa atttggaaac cggatcaaag tgctgagaag tcgaattcgc ttcatccatt 4441 tggtcacagg ttgtgtcctg ggctcctcgg gaaaggttct gcccaagtgg ggctgggagc 4501 agttggaagt tacttgcacc ccatacctga aagaaaccct caactccatc tggaatgtgg 4561 aggaccatat caatcccaag ttgccaaaca tcagcctgga tgtgctacag cccagttttc 4621 ctgagatctt gctggaatcc cacatggtca tgatccgggg gaacagtggc ctcaaaccca 4681 aggacaatga gttcacgtcc aaaccctggc actggcctat caactatcag ggcctacgct 4741 tctcaggggt caatgacaca gatttccgag tctatctgct tggcaacccg gtggtttggt 4801 ggctgaatct gttgagcatc gccctctacc tcctctcagg gagcatcatt gctgtagcca 4861 tgcagagagg ggcacggctg ccagcggagg ttgcagggtt gtcccaggtc ctgctgcgag 4921 gaggcggcca ggtcctgctc ggctggacac tccattactt cccgtttttc ctgatgggcc 4981 gggtcctcta cttccaccac tacttcccag ccatgctctt ctcaagcatg ttgacaggca 5041 ttctgtggga caccctcctg cggctctgtg cctggggctt ggcctcatgg cccctggcga 5101 ggggcataca tgtggcggga atcctgagcc tgctcctggg aactgcctac agcttctacc 5161 tcttccaccc tctggcttac gggatggttg gtcccctggc ccaggacccc caaagtccaa 5221 tggcaggact aaggtggctg gactcatggg actttgaaaa cttgtacttt caaggctCCA 5281 GCTTTCTTGT ACAAAGTGGT GATGGCCGCC ATCATCATCA TCATCATCAT CATGCGTGGA 5341 GCCACCCGCA GTTCGAAAAA TAATAAGGAT CCAATCAACC TCTGGATTAC AAAATTTGTG 5401 AAAGATTGAC TGGTATTCTT AACTATGTTG CTCCTTTTAC GCTATGTGGA TACGCTGCTT 5461 TAATGCCTTT GTATCATGCT ATTGCTTCCC GTATGGCTTT CATTTTCTCC TCCTTGTATA 5521 AATCCTGGTT GCTGTCTCTT TATGAGGAGT TGTGGCCCGT TGTCAGGCAA CGTGGCGTGG 5581 TGTGCACTGT GTTTGCTGAC GCAACCCCCA CTGGTTGGGG CATTGCCACC ACCTGTCAGC 5641 TCCTTTCCGG GACTTTCGCT TTCCCCCTCC CTATTGCCAC GGCGGAACTC ATCGCCGCCT 5701 GCCTTGCCCG CTGCTGGACA GGGGCTCGGC TGTTGGGCAC TGACAATTCC GTGGTGTTGT 5761 CGGGGAAGCT GACGTCCTTT CCATGGCTGC TCGCCTGTGT TGCCACCTGG ATTCTGCGCG 5821 GGACGTCCTT CTGCTACGTC CCTTCGGCCC TCAATCCAGC GGACCTTCCT TCCCGCGGCC 5881 TGCTGCCGGC TCTGCGGCCT CTTCCGCGTC TTCGAGATCT GCCTCGACTG TGCCTTCTAG 5941 TTGCCAGCCA TCTGTTGTTT GCCCCTCCCC CGTGCCTTCC TTGACCCTGG AAGGTGCCAC 6001 TCCCACTGTC CTTTCCTAAT AAAATGAGGA AATTGCATCG CATTGTCTGA GTAGGTGTCA 6061 TTCTATTCTG GGGGGTGGGG TGGGGCAGGA CAGCAAGGGG GAGGATTGGG AAGACAATAG 6121 CAGGCATGCT GGGGACTCGA GTTAAGGGCG AATTGCCGAT TAGGATCTTC CTAGAGCATG 6181 GCTACGTAGA TAAGTAGCAT GGCGGGTTAA TCATTAACTA CAAGGAACCC CTAGTGATGG 6241 AGTTGGCCAC TCCCTCTCTG CGCGCTCGCT CGCTCACTGA GGCCGGGCGA CCAAAGGTCG 6301 CCCGACGCCC GGGCTTTGCC CGGGCGGCCT CAGTGAGCGA GCGAGCGCGC AGCCTTAATT 6361 AAGAGCTCAT GGCGCGCCTA GGCCTTGACG GCCTTCCGCC AATTCGCCCT ATAGTGAGTC 6421 GTATTACGTC GCGCTCACTG GCCGTCGTTT TACAACGTCG TGACTGGGAA AACCCTGGCG 6481 TTACCCAACT TAATCGCCTT GCAGCACATC CCCCTTTCGC CAGCTGGCGT AATAGCGAAG 6541 AGGCCCGCAC CGAAACGCCC TTCCCAACAG TTGCGCAGCC TGAATGGCGA ATGGGAGCGC 6601 CCTGTAGCGG CCACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC 6661 GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTTAA 6721 CAAAATATTA ACGCTTACAA TTTAG //