LOCUS OGT-pGEn1-DEST 7645 bp DNA circular 22-JAN-2013 FEATURES Location/Qualifiers CDS 129..1108 /dnas_title="AmpR" /label=AmpR /vntifkey="4" rep_origin 1137..1804 /dnas_title="Col E1 origin" /label=Col E1 origin /vntifkey="33" misc_feature 1998..2127 /note="5'ITR" misc_feature 2208..2790 /note="CMV Promoter" misc_feature 2828..2960 /note="Artificial Intron" misc_feature 2977..3054 /note="TCM Sig Seq" misc_recomb complement(3114..3142) /label=attB1 /note="attB1 site" /note="recognized as attB1" misc_feature 3055..3078 /note="8xHis Tag" misc_feature 3085..3108 /note="StrepTag" misc_feature 6273..6814 /note="WPRE" misc_recomb 6235..6257 /label=attB2 /note="attB2 site" /note="recognized as attB2" misc_feature complement(6821..7035) /note="BGH pA" misc_feature complement(7123..7252) /note="3'-ITR" misc_feature 3143..3165 /note="TEV site" source 3166..6228 /dnas_title="OGT-pGEn1-DEST" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:22921 IMAGE:4865031" /tissue_type="Colon, adenocarcinoma" /clone_lib="NIH_MGC_15" /lab_host="DH10B-R" /note="Vector: pOTB7" gene 3166..6228 /dnas_title="OGT" /gene="OGT" /gene_synonym="FLJ23071" /gene_synonym="HRNT1" /gene_synonym="MGC22921" /gene_synonym="O-GLCNAC" /db_xref="GeneID:8473" /db_xref="HGNC:8127" /db_xref="MIM:300255" CDS 3166..6228 /dnas_title="O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase)" /gene="OGT" /gene_synonym="FLJ23071" /gene_synonym="HRNT1" /gene_synonym="MGC22921" /gene_synonym="O-GLCNAC" /codon_start=1 /product="O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase)" /protein_id="AAH14434.1" /db_xref="GI:15680175" /db_xref="GeneID:8473" /db_xref="HGNC:8127" /db_xref="MIM:300255" /translation="MASSVGNVADSTGLAELAHREYQAGDFEAAERHCMQLWRQEPDN TGVLLLLSSIHFQCRRLDRSAHFSTLAIKQNPLLAEAYSNLGNVYKERGQLQEAIEHY RHALRLKPDFIDGYINLAAALVAAGDMEGAVQAYVSALQYNPDLYCVRSDLGNLLKAL GRLEEAKACYLKAIETQPNFAVAWSNLGCVFNAQGEIWLAIHHFEKAVTLDPNFLDAY INLGNVLKEARIFDRAVAAYLRALSLSPNHAVVHGNLACVYYEQGLIDLAIDTYRRAI ELQPHFPDAYCNLANALKEKGSVAEAEDCYNTALRLCPTHADSLNNLANIKREQGNIE EAVRLYRKALEVFPEFAAAHSNLASVLQQQGKLQEALMHYKEAIRISPTFADAYSNMG NTLKEMQDVQGALQCYTRAIQINPAFADAHSNLASIHKDSGNIPEAIASYRTALKLKP DFPDAYCNLAHCLQIVCDWTDYDERMKKLVSIVADQLEKNRLPSVHPHHSMLYPLSHG FRKAIAERHGNLCLDKINVLHKPPYEHPKDLKLSDGRLRVGYVSSDFGNHPTSHLMQS IPGMHNPDKFEVFCYALSPDDGTNFRVKVMAEANHFIDLSQIPCNGKAADRIHQDGIH ILVNMNGYTKGARNELFALRPAPIQAMWLGYPGTSGALFMDYIITDQETSPAEVAEQY SEKLAYMPHTFFIGDHANMFPHLKKKAVIDFKSNGHIYDNRIVLNGIDLKAFLDSLPD VKIVKMKCPDGGDNADSSNTALNMPVIPMNTIAEAVIEMINRGQIQITINGFSISNGL ATTQINNKAATGEEVPRTIIVTTRSQYGLPEDAIVYCNFNQLYKIDPSTLQMWANILK RVPNSVLWLLRFPAVGEPNIQQYAQNMGLPQNRIIFSPVAPKEEHVRRGQLADVCLDT PLCNGHTTGMDVLWAGTPMVTMPGETLASRVAASQLTCLGCLELIAKNRQEYEDIAVK LGTDLEYLKKVRGKVWKQRISSPLFNTKQYTMELERLYLQMWEHYAAGNKPDHMIKPV EVTESA" misc_feature 3166..6228 /note="OGT coding region" vector join(6243..7645,1..3132) /source="pGEn1-DEST" /type="Destination Vector" clone 3133..6242 /source="OGT-Nterm-TEVfusion-pDONR221" /type="Entry Clone" ORIGIN 1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA 1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA 1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG 1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG 2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG 2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT 2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG 2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT 2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG 2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT 2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG 2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG 2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC 2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC 2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT 2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA 2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCGCCATGA GACTGCTGAC AGCCCTGTTC 3001 GCCTACTTCA TCGTGGCCCT GATCCTGGCC TTCAGCGTGT CCGCCAAGAG CATGCACCAC 3061 CACCATCACC ATCATCACAG CGCCTGGTCC CACCCCCAGT TCGAGAAAGA ATTATCAACA 3121 AGTTTGTACA AAAAAGCAGG CTctgaaaac ttgtactttc aaggcgagtt ggcacatcga 3181 gaatatcagg caggagattt tgaggcagct gagagacact gcatgcagct ctggagacaa 3241 gagccagaca atactggtgt gcttttatta ctttcatcta tacacttcca gtgtcgaagg 3301 ctggacagat ctgctcactt tagcactctg gcaattaaac agaaccccct tctggcagaa 3361 gcttattcga atttggggaa tgtgtacaag gaaagagggc agttgcagga ggcaattgag 3421 cattatcgac atgcattgcg tctcaaacct gatttcatcg atggttatat taacctggca 3481 gccgccttgg tagcagcggg tgacatggaa ggggcagtac aagcttacgt ctctgctctt 3541 cagtacaatc ctgatttgta ctgtgttcgc agtgacctgg ggaacctgct caaagccctg 3601 ggtcgcttgg aagaagccaa ggcatgttat ttgaaagcaa ttgagacgca accgaacttt 3661 gcagtagctt ggagtaatct tggctgtgtt ttcaatgcac aaggggaaat ttggcttgca 3721 attcatcact ttgaaaaggc tgtcaccctt gacccaaact ttctggatgc ttatatcaat 3781 ttaggaaatg tcttgaaaga ggcacgcatt tttgacagag ctgtggcagc ttatcttcgt 3841 gccctaagtt tgagtccaaa tcacgcagtg gtgcacggca acctggcttg tgtatactat 3901 gagcaaggcc tgatagatct ggcaatagac acctacaggc gggctatcga actacaacca 3961 catttccctg atgcttactg caacctagcc aatgctctca aagagaaggg cagtgttgct 4021 gaagcagaag attgttataa tacagctctc cgtctgtgtc ccacccatgc agactctctg 4081 aataacctag ccaatatcaa acgagaacag ggaaacattg aagaggcagt tcgcttgtat 4141 cgtaaagcat tagaagtctt cccagagttt gctgctgccc attcaaattt agcaagtgta 4201 ctgcagcagc agggaaaact gcaggaagct ctgatgcatt ataaggaggc tattcgaatc 4261 agtcctacct ttgctgatgc ctactctaat atgggaaaca ctctaaagga gatgcaggat 4321 gttcagggag ccttgcagtg ttatacgcgt gccatccaaa ttaatcctgc atttgcagat 4381 gcacatagca atctggcttc cattcataag gattcaggga atattccaga agccatagct 4441 tcttaccgca cggctctgaa acttaagcct gattttcctg atgcttattg taacttggct 4501 cattgcctgc agattgtctg tgattggaca gactatgatg agcgaatgaa gaagttggtc 4561 agtattgtgg ctgaccagtt agagaagaat aggttgcctt ctgtgcatcc tcatcatagt 4621 atgctatatc ctctttctca tggcttcagg aaggctattg ctgagaggca cggcaacctg 4681 tgcttagata agattaatgt tcttcataaa ccaccatatg aacatccaaa agacttgaag 4741 ctcagtgatg gtcggctgcg tgtaggatat gtgagttccg actttgggaa tcatcctact 4801 tctcacctta tgcagtctat tccaggcatg cacaatcctg ataaatttga ggtgttctgt 4861 tatgccctga gcccagacga tggcacaaac ttccgagtga aggtgatggc agaagccaat 4921 catttcattg atctttctca gattccatgc aatggaaaag cagctgatcg catccatcag 4981 gatggaattc atatccttgt aaatatgaat ggctatacta agggcgctcg aaatgagctt 5041 tttgctctca ggccagctcc tattcaggca atgtggctgg gataccctgg gacgagtggt 5101 gcgcttttca tggattatat tatcactgat caggaaactt cgccagctga agttgctgag 5161 cagtattccg agaaattggc ttatatgccc cacacttttt ttattggtga tcatgctaat 5221 atgttccctc acctgaagaa aaaagcagtc atcgatttta agtccaatgg gcacatttat 5281 gacaatcgga tagttctgaa tggcatcgac ctcaaagcat ttcttgatag tctaccagat 5341 gtgaaaattg tcaagatgaa gtgtcctgat ggaggagaca atgcagatag cagtaacaca 5401 gctcttaata tgcctgttat tcctatgaat actattgcag aagcagttat tgaaatgatt 5461 aaccgaggac agattcaaat aacaattaat ggattcagta ttagcaatgg actggcaact 5521 actcagatca acaataaggc tgcaactgga gaggaggttc cccgtaccat tattgtaacc 5581 acccgttctc agtacgggtt accagaagat gccatcgtat actgtaactt taatcagttg 5641 tataaaattg acccttctac tttgcagatg tgggcaaaca ttctgaagcg tgttcccaat 5701 agtgtactct ggctgttgcg ttttccagca gtaggagaac ctaatattca acagtatgca 5761 caaaacatgg gcctgcccca gaaccgtatc attttttcac ctgttgctcc taaagaggaa 5821 cacgtcagga gaggccagct ggctgatgtc tgcttggaca ctccactctg taatgggcac 5881 accacaggga tggatgtcct ctgggcaggg acccccatgg tgactatgcc aggagagact 5941 cttgcttctc gagttgcagc atcccagctc acttgcttag gttgtcttga gcttattgct 6001 aaaaacagac aagaatatga agacatagct gtgaagctgg gaactgatct agaatacctg 6061 aagaaagttc gtggcaaagt ctggaagcaa agaatatcta gccctctgtt caacaccaaa 6121 caatacacaa tggaactaga gcggctctat ctacagatgt gggagcatta tgcagctggc 6181 aacaaacctg accacatgat taagcctgtt gaagtcactg agtcagcaTA GGACCCAGCT 6241 TTCTTGTACA AAGTGGTTGA TAGCTTGGAT CCAATCAACC TCTGGATTAC AAAATTTGTG 6301 AAAGATTGAC TGGTATTCTT AACTATGTTG CTCCTTTTAC GCTATGTGGA TACGCTGCTT 6361 TAATGCCTTT GTATCATGCT ATTGCTTCCC GTATGGCTTT CATTTTCTCC TCCTTGTATA 6421 AATCCTGGTT GCTGTCTCTT TATGAGGAGT TGTGGCCCGT TGTCAGGCAA CGTGGCGTGG 6481 TGTGCACTGT GTTTGCTGAC GCAACCCCCA CTGGTTGGGG CATTGCCACC ACCTGTCAGC 6541 TCCTTTCCGG GACTTTCGCT TTCCCCCTCC CTATTGCCAC GGCGGAACTC ATCGCCGCCT 6601 GCCTTGCCCG CTGCTGGACA GGGGCTCGGC TGTTGGGCAC TGACAATTCC GTGGTGTTGT 6661 CGGGGAAGCT GACGTCCTTT CCATGGCTGC TCGCCTGTGT TGCCACCTGG ATTCTGCGCG 6721 GGACGTCCTT CTGCTACGTC CCTTCGGCCC TCAATCCAGC GGACCTTCCT TCCCGCGGCC 6781 TGCTGCCGGC TCTGCGGCCT CTTCCGCGTC TTCGAGATCT GCCTCGACTG TGCCTTCTAG 6841 TTGCCAGCCA TCTGTTGTTT GCCCCTCCCC CGTGCCTTCC TTGACCCTGG AAGGTGCCAC 6901 TCCCACTGTC CTTTCCTAAT AAAATGAGGA AATTGCATCG CATTGTCTGA GTAGGTGTCA 6961 TTCTATTCTG GGGGGTGGGG TGGGGCAGGA CAGCAAGGGG GAGGATTGGG AAGACAATAG 7021 CAGGCATGCT GGGGACTCGA GTTAAGGGCG AATTGCCGAT TAGGATCTTC CTAGAGCATG 7081 GCTACGTAGA TAAGTAGCAT GGCGGGTTAA TCATTAACTA CAAGGAACCC CTAGTGATGG 7141 AGTTGGCCAC TCCCTCTCTG CGCGCTCGCT CGCTCACTGA GGCCGGGCGA CCAAAGGTCG 7201 CCCGACGCCC GGGCTTTGCC CGGGCGGCCT CAGTGAGCGA GCGAGCGCGC AGCCTTAATT 7261 AAGAGCTCAT GGCGCGCCTA GGCCTTGACG GCCTTCCGCC AATTCGCCCT ATAGTGAGTC 7321 GTATTACGTC GCGCTCACTG GCCGTCGTTT TACAACGTCG TGACTGGGAA AACCCTGGCG 7381 TTACCCAACT TAATCGCCTT GCAGCACATC CCCCTTTCGC CAGCTGGCGT AATAGCGAAG 7441 AGGCCCGCAC CGAAACGCCC TTCCCAACAG TTGCGCAGCC TGAATGGCGA ATGGGAGCGC 7501 CCTGTAGCGG CCACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC 7561 GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTTAA 7621 CAAAATATTA ACGCTTACAA TTTAG //